P - Baseplate hub protein gp44 - Escherichia phage Mu

Functionⁱ

Forms the central cylindrical hub of the baseplate and plays an important role in baseplate and tail assembly (Probable). Core component of the initiator complex that triggers the tail tube polymerization during tail assembly (Probable). Forms a conduit that probably functions as an extension of the tail tube allowing viral DNA release during ejection. Might facilitate the interaction of the virus with the host cell surface through electrostatic interactions during virus entry into host cell.Curated

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	209 – 228	H-T-H motifSequence analysisAdd BLAST		20

GO - Biological processⁱ

viral genome ejection through host cell envelope, contractile tail mechanism Source: UniProtKB-KW
viral tail assembly Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Viral contractile tail ejection system
Biological process	Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral release from host cell, Viral tail assembly, Virus entry into host cell

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Baseplate hub protein gp44 Alternative name(s): 43 kDa tail protein Gene product 44 Short name: gp44 Gene product P Short name: gpP
Gene namesⁱ	Name:P Ordered Locus Names:Mup44
Organismⁱ	Escherichia phage Mu (Bacteriophage Mu)
Taxonomic identifierⁱ	10677 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Muvirus
Virus hostⁱ	Enterobacteriaceae [TaxID: 543]
Proteomesⁱ	UP000002611 Componentⁱ: Genome

Subcellular locationⁱ

Note:

Baseplate protein.1 Publication

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
virus tail, baseplate Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechⁱ

Disruption phenotypeⁱ

No tail is synthesized.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000077692	1 – 379	Baseplate hub protein gp44Add BLAST		379

Post-translational modificationⁱ

Cleavage of the C-terminus gives rise to a shorter 40 kDa form.

Expressionⁱ

Inductionⁱ

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stageⁱ

Late protein

Interactionⁱ

Subunit structureⁱ

Heterotrimer of one uncleaved (42 kDa) and two cleaved (40 kDa) forms. Forms a hub-like structure with an inner diameter of 25 Angstroms through which DNA can presumably pass during infection. Part of a complex composed of three DNA circularization protein N, three baseplate hub protein gp44 and three sub-complex wedge (made of two copies of each baseplate protein gp46, gp47 and gp48) that forms the baseplate (PubMed:27555589).3 Publications

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	5 – 9	Combined sources	5
Beta strandⁱ	12 – 15	Combined sources	4
Beta strandⁱ	18 – 25	Combined sources	8
Beta strandⁱ	32 – 39	Combined sources	8
Helixⁱ	46 – 48	Combined sources	3
Beta strandⁱ	53 – 58	Combined sources	6
Beta strandⁱ	61 – 75	Combined sources	15
Beta strandⁱ	80 – 88	Combined sources	9
Helixⁱ	91 – 95	Combined sources	5
Beta strandⁱ	101 – 108	Combined sources	8
Helixⁱ	110 – 118	Combined sources	9
Helixⁱ	119 – 121	Combined sources	3
Beta strandⁱ	125 – 127	Combined sources	3
Helixⁱ	132 – 135	Combined sources	4
Beta strandⁱ	138 – 142	Combined sources	5
Helixⁱ	149 – 158	Combined sources	10
Turnⁱ	159 – 161	Combined sources	3
Beta strandⁱ	163 – 166	Combined sources	4
Helixⁱ	168 – 170	Combined sources	3
Beta strandⁱ	172 – 175	Combined sources	4
Beta strandⁱ	180 – 187	Combined sources	8
Turnⁱ	188 – 190	Combined sources	3
Beta strandⁱ	191 – 198	Combined sources	8
Beta strandⁱ	205 – 209	Combined sources	5
Beta strandⁱ	233 – 236	Combined sources	4
Beta strandⁱ	245 – 248	Combined sources	4
Helixⁱ	256 – 274	Combined sources	19
Beta strandⁱ	275 – 285	Combined sources	11
Beta strandⁱ	297 – 302	Combined sources	6
Helixⁱ	303 – 305	Combined sources	3
Beta strandⁱ	312 – 322	Combined sources	11
Turnⁱ	323 – 325	Combined sources	3
Beta strandⁱ	326 – 335	Combined sources	10
Helixⁱ	336 – 339	Combined sources	4

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1WRU	X-ray	2.10	A	1-379	[»]
ProteinModelPortalⁱ	P08558
SMRⁱ	P08558
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P08558

EvolutionaryTraceⁱ

P08558

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	216 – 224	DisorderedSequence analysis		9
Regionⁱ	345 – 379	DisorderedSequence analysisAdd BLAST		35

Domainⁱ

C-terminal region forms a flexible domain.Curated

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090000CA

OrthoDBⁱ

VOG090000CA

Family and domain databases

Gene3Dⁱ	3.30.1920.10, 1 hit
InterProⁱ	View protein in InterPro IPR023399 Baseplate-like_2-layer_sand IPR026276 Baseplate_GpP
PIRSFⁱ	PIRSF004440 GpP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P08558-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSNTVTLRAD GRLFTGWTSV SVTRSIESVA GYFELGVNVP PGTDLSGLAP 
        60         70         80         90        100
GKKFTLEIGG QIVCTGYIDS RRRQMTADSM KITVAGRDKT ADLIDCAAVY 
       110        120        130        140        150
SGGQWKNRTL EQIARDLCAP YGVTVRWELS DKESSAAFPG FTLDHSETVY 
       160        170        180        190        200
EALVRASRAR GVLMTSNAAG ELVFSRAAST ATDELVLGEN LLTLDFEEDF 
       210        220        230        240        250
RDRFSEYTVK GYARANGAEG DDIDAKSIVS RKGTATDSDV TRYRPMIIIA 
       260        270        280        290        300
DSKITAKDAQ ARALREQRRR LAKSITFEAE IDGWTRKDGQ LWMPNLLVTI 
       310        320        330        340        350
DASKYAIKTT ELLVSKVTLI LNDQDGLKTR VSLAPREGFL VPVESDRKNR 
       360        370 
KGGDSNGGID ALVEDYYRRH PEKTPPWKE

Length:379

Mass (Da):41,785

Last modified:August 1, 1988 - v1

Checksum:ⁱE09AED2158D13CA1

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X06796 Genomic DNA Translation: CAA29956.1 AF083977 Genomic DNA Translation: AAF01122.1
PIRⁱ	S01891 ZPBPMU
NCBI Reference Sequences More...RefSeqⁱ	NP_050648.1, NC_000929.1

Genome annotation databases

GeneIDⁱ	2636285
KEGGⁱ	vg:2636285

Protein	Similar proteins		Species	Score	Length
P08558	Putative tail protein		ECOLW		379
UPI000BE59DD0		ECOLX		290
Bacteriophage Mu P protein		Escherichia coli TA447		379
UPI000D02DE94		Escherichia sp. R18		379
Bacteriophage Mu P protein		Escherichia albertii B156		379
+77

Protein	Similar proteins		Species	Score	Length
P08558	Putative tail protein		ECOLW		379
UPI000BE59DD0		ECOLX		290
UPI00056F1574		Escherichia marmotae		379
Bacteriophage Mu P protein		Escherichia albertii B156		379
Bacteriophage Mu P protein		Escherichia coli TA447		379
+91

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X06796 Genomic DNA Translation: CAA29956.1 AF083977 Genomic DNA Translation: AAF01122.1
PIRⁱ	S01891 ZPBPMU
NCBI Reference Sequences More...RefSeqⁱ	NP_050648.1, NC_000929.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1WRU	X-ray	2.10	A	1-379	[»]
ProteinModelPortalⁱ	P08558
SMRⁱ	P08558
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	2636285
KEGGⁱ	vg:2636285

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090000CA

OrthoDBⁱ

VOG090000CA

Miscellaneous databases

EvolutionaryTraceⁱ	P08558

EvolutionaryTraceⁱ

P08558

Family and domain databases

Gene3Dⁱ	3.30.1920.10, 1 hit
InterProⁱ	View protein in InterPro IPR023399 Baseplate-like_2-layer_sand IPR026276 Baseplate_GpP
PIRSFⁱ	PIRSF004440 GpP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BP44_BPMU
Accessionⁱ	P08558Primary (citable) accession number: P08558
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	August 1, 1988
	Last modified:	November 22, 2017
	This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08558 (BP44_BPMU)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Baseplate hub protein gp44

P

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ