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Protein

Neurofilament medium polypeptide

Gene

Nefm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament medium polypeptide
Short name:
NF-M
Alternative name(s):
160 kDa neurofilament protein
Neurofilament 3
Neurofilament triplet M protein
Gene namesi
Name:Nefm
Synonyms:Nef3, Nfm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97314 Nefm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000637952 – 848Neurofilament medium polypeptideAdd BLAST847

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei30PhosphoserineBy similarity1
Modified residuei42Omega-N-methylarginineCombined sources1
Glycosylationi47O-linked (GlcNAc) threonine1 Publication1
Modified residuei97PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei318PhosphotyrosineCombined sources1
Modified residuei344PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei428PhosphoserineBy similarity1
Glycosylationi430O-linked (GlcNAc) threonine1 Publication1
Modified residuei466PhosphoserineBy similarity1
Modified residuei482PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1
Modified residuei506PhosphoserineBy similarity1
Modified residuei537PhosphoserineBy similarity1
Modified residuei545PhosphoserineBy similarity1
Modified residuei550PhosphoserineCombined sources1
Modified residuei551PhosphoserineCombined sources1
Modified residuei565PhosphothreonineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei610PhosphoserineCombined sources1
Modified residuei642PhosphothreonineCombined sources1
Modified residuei645PhosphoserineBy similarity1
Modified residuei669PhosphoserineCombined sources1
Modified residuei689PhosphoserineBy similarity1
Modified residuei715PhosphoserineCombined sources1
Modified residuei723PhosphoserineCombined sources1
Modified residuei753PhosphoserineBy similarity1
Modified residuei769PhosphoserineCombined sources1

Post-translational modificationi

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

EPDiP08553
MaxQBiP08553
PaxDbiP08553
PeptideAtlasiP08553
PRIDEiP08553

2D gel databases

UCD-2DPAGEiP08553

PTM databases

iPTMnetiP08553
PhosphoSitePlusiP08553
SwissPalmiP08553

Expressioni

Gene expression databases

CleanExiMM_NEFM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201758, 10 interactors
IntActiP08553, 14 interactors
MINTiP08553
STRINGi10090.ENSMUSP00000022638

Structurei

3D structure databases

ProteinModelPortaliP08553
SMRiP08553
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 410IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 102HeadAdd BLAST101
Regioni103 – 134Coil 1AAdd BLAST32
Regioni135 – 147Linker 1Add BLAST13
Regioni148 – 246Coil 1BAdd BLAST99
Regioni247 – 263Linker 12Add BLAST17
Regioni264 – 285Coil 2AAdd BLAST22
Regioni286 – 289Linker 24
Regioni290 – 410Coil 2BAdd BLAST121
Regioni411 – 848TailAdd BLAST438

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME Eukaryota
ENOG410XPTM LUCA
HOVERGENiHBG013015
InParanoidiP08553
KOiK04573
PhylomeDBiP08553
TreeFamiTF330122

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
IPR002957 Keratin_I
IPR027697 NF-M
PANTHERiPTHR23239 PTHR23239, 2 hits
PTHR23239:SF19 PTHR23239:SF19, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
PRINTSiPR01248 TYPE1KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P08553-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSYTLDSLGN PSAYRRVTET RSSFSRVSGS PSSGFRSQSW SRGSPSTVSS
60 70 80 90 100
SYKRSALAPR LAYSSAMLSS AESSLDFSQS SSLLNGGSGG DYKLSRSNEK
110 120 130 140 150
EQLQGLNDRF AGYIEKVHYL EQQNKEIEAE IQALRQKQAS HAQLGDAYDQ
160 170 180 190 200
EIRELRATLE MVNHEKAQVQ LDSDHLEEDI HRLKERFEEE ARLRDDTEAA
210 220 230 240 250
IRALRKDIEE SSMVKVELDK KVQSLQDEVA FLRSNHEEEV ADLLAQIQAS
260 270 280 290 300
HITVERKDYL KTDISTALKE IRSQLECHSD QNMHQAEEWF KCRYAKLTEA
310 320 330 340 350
AEQNKEAIRS AKEEIAEYRR QLQSKSIELE SVRGTKESLE RQLSDIEERH
360 370 380 390 400
NHDLSSYQDT IQQLENELRG TKWEMARHLR EYQDLLNVKM ALDIEIAAYR
410 420 430 440 450
KLLEGEETRF STFSGSITGP LYTHRQPSVT ISSKIQKTKV EAPKLKVQHK
460 470 480 490 500
FVEEIIEETK VEDEKSEMEE TLTAIAEELA ASAKEEKEEA EEKEEEPEAE
510 520 530 540 550
KSPVKSPEAK EEEEEGEKEE EEEGQEEEEE EDEGVKSDQA EEGGSEKEGS
560 570 580 590 600
SEKDEGEQEE EEGETEAEGE GEEAEAKEEK KIEGKVEEVA VKEEIKVEKP
610 620 630 640 650
EKAKSPMPKS PVEEVKPKPE AKAGKGEQKE EEKVEEEKKE VTKESPKEEK
660 670 680 690 700
VEKKEEKPKD VADKKKAESP VKEKAVEEVI TISKSVKVSL EKDTKEEKPQ
710 720 730 740 750
PQEKVKEKAE EEGGSEEEGS DRSPQESKKE DIAINGEVEG KEEEEQETQE
760 770 780 790 800
KGSGREEEKG VVTNGLDVSP AEEKKGEDSS DDKVVVTKKV EKITSEGGDG
810 820 830 840
ATKYITKSVT VTQKVEEHEE TFEEKLVSTK KVEKVTSHAI VKEVTQGD
Length:848
Mass (Da):95,916
Last modified:May 1, 2007 - v4
Checksum:i0783F50558A7D4C3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J036A0A0R4J036_MOUSE
Neurofilament 3, medium
Nefm Nef3, mCG_2494
848Annotation score:
D3YZ35D3YZ35_MOUSE
Neurofilament medium polypeptide
Nefm
470Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17V → VP in CAA29127 (PubMed:3036526).Curated1
Sequence conflicti53K → T in CAA29127 (PubMed:3036526).Curated1
Sequence conflicti57L → V in CAA29127 (PubMed:3036526).Curated1
Sequence conflicti234S → R in CAA29127 (PubMed:3036526).Curated1
Sequence conflicti432S → F in AAA39815 (PubMed:3103856).Curated1
Sequence conflicti539 – 540QA → RR in AAA39815 (PubMed:3103856).Curated2
Sequence conflicti628Q → H in ABA46749 (Ref. 2) Curated1
Sequence conflicti628Q → H in BAC34724 (PubMed:16141072).Curated1
Sequence conflicti628Q → H in BAE37734 (PubMed:16141072).Curated1
Sequence conflicti696E → K in BAC34724 (PubMed:16141072).Curated1
Sequence conflicti699P → L in ABA46749 (Ref. 2) Curated1
Sequence conflicti699P → L in BAC34724 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05640 Genomic DNA Translation: CAA29127.1
DQ201636 mRNA Translation: ABA46749.1
AK051696 mRNA Translation: BAC34724.1
AK164318 mRNA Translation: BAE37734.1
AK165041 mRNA Translation: BAE38014.1
BC119602 mRNA Translation: AAI19603.1
BC128564 mRNA Translation: AAI28565.1
M20481 mRNA Translation: AAA39815.1
CCDSiCCDS27233.1
PIRiB43772
S00030
RefSeqiNP_032717.2, NM_008691.2
UniGeneiMm.242832
Mm.390700

Genome annotation databases

GeneIDi18040
KEGGimmu:18040
UCSCiuc007ulo.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05640 Genomic DNA Translation: CAA29127.1
DQ201636 mRNA Translation: ABA46749.1
AK051696 mRNA Translation: BAC34724.1
AK164318 mRNA Translation: BAE37734.1
AK165041 mRNA Translation: BAE38014.1
BC119602 mRNA Translation: AAI19603.1
BC128564 mRNA Translation: AAI28565.1
M20481 mRNA Translation: AAA39815.1
CCDSiCCDS27233.1
PIRiB43772
S00030
RefSeqiNP_032717.2, NM_008691.2
UniGeneiMm.242832
Mm.390700

3D structure databases

ProteinModelPortaliP08553
SMRiP08553
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201758, 10 interactors
IntActiP08553, 14 interactors
MINTiP08553
STRINGi10090.ENSMUSP00000022638

PTM databases

iPTMnetiP08553
PhosphoSitePlusiP08553
SwissPalmiP08553

2D gel databases

UCD-2DPAGEiP08553

Proteomic databases

EPDiP08553
MaxQBiP08553
PaxDbiP08553
PeptideAtlasiP08553
PRIDEiP08553

Protocols and materials databases

DNASUi18040
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18040
KEGGimmu:18040
UCSCiuc007ulo.1 mouse

Organism-specific databases

CTDi4741
MGIiMGI:97314 Nefm

Phylogenomic databases

eggNOGiENOG410IGME Eukaryota
ENOG410XPTM LUCA
HOVERGENiHBG013015
InParanoidiP08553
KOiK04573
PhylomeDBiP08553
TreeFamiTF330122

Miscellaneous databases

PROiPR:P08553
SOURCEiSearch...

Gene expression databases

CleanExiMM_NEFM

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
IPR002957 Keratin_I
IPR027697 NF-M
PANTHERiPTHR23239 PTHR23239, 2 hits
PTHR23239:SF19 PTHR23239:SF19, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
PRINTSiPR01248 TYPE1KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFM_MOUSE
AccessioniPrimary (citable) accession number: P08553
Secondary accession number(s): A2VCT5
, Q0VDM8, Q3HRJ6, Q3TNS4, Q3TPK2, Q61961, Q8BQ20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 181 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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