UniProtKB - P08525 (QCR8_YEAST)
Cytochrome b-c1 complex subunit 8, mitochondrial
QCR8
Functioni
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.
1 PublicationMiscellaneous
GO - Biological processi
- aerobic respiration Source: SGD
- cellular respiration Source: ComplexPortal
- mitochondrial electron transport, ubiquinol to cytochrome c Source: ComplexPortal
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-c1 complex subunit 8, mitochondrialAlternative name(s): Complex III subunit 8 Complex III subunit VII Ubiquinol-cytochrome c oxidoreductase subunit 8 Ubiquinol-cytochrome c reductase complex 11 kDa protein Ubiquinone-binding protein QP-C |
Gene namesi | Name:QCR8 Ordered Locus Names:YJL166W ORF Names:J0526 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003702, QCR8 |
VEuPathDBi | FungiDB:YJL166W |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Mitochondrion
- mitochondrial inner membrane Source: ComplexPortal
- mitochondrial respiratory chain complex III Source: SGD
- mitochondrion Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 49 | Mitochondrial matrix2 PublicationsAdd BLAST | 48 | |
Transmembranei | 50 – 80 | Helical2 PublicationsAdd BLAST | 31 | |
Topological domaini | 81 – 94 | Mitochondrial intermembrane2 PublicationsAdd BLAST | 14 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000193551 | 2 – 94 | Cytochrome b-c1 complex subunit 8, mitochondrialAdd BLAST | 93 |
Proteomic databases
MaxQBi | P08525 |
PaxDbi | P08525 |
PRIDEi | P08525 |
TopDownProteomicsi | P08525 |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 10 subunits. The complex is composed of 3 respiratory subunits cytochrome b (COB), cytochrome c1 (CYT1) and Rieske protein (RIP1), 2 core protein subunits COR1 and QCR2, and 5 low-molecular weight protein subunits QCR6, QCR7, QCR8, QCR9 and QCR10 (PubMed:10873857, PubMed:11880631, PubMed:18390544, PubMed:30598554). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a monomer or a dimer of cytochrome c oxidase (complex IV, CIV), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554).
7 PublicationsProtein-protein interaction databases
BioGRIDi | 33593, 319 interactors |
ComplexPortali | CPX-567, Mitochondrial respiratory chain complex III |
DIPi | DIP-4706N |
IntActi | P08525, 5 interactors |
MINTi | P08525 |
STRINGi | 4932.YJL166W |
Miscellaneous databases
RNActi | P08525, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P08525 |
SMRi | P08525 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08525 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4116, Eukaryota |
GeneTreei | ENSGT00390000004029 |
HOGENOMi | CLU_156007_0_1_1 |
InParanoidi | P08525 |
OMAi | AAIFNTW |
Family and domain databases
Gene3Di | 1.20.5.210, 1 hit |
InterProi | View protein in InterPro IPR004205, Cyt_bc1_su8 IPR036642, Cyt_bc1_su8_sf |
PANTHERi | PTHR12119, PTHR12119, 1 hit |
Pfami | View protein in Pfam PF02939, UcrQ, 1 hit |
SUPFAMi | SSF81508, SSF81508, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGPPSGKTYM GWWGHMGGPK QKGITSYAVS PYAQKPLQGI FHNAVFNSFR
60 70 80 90
RFKSQFLYVL IPAGIYWYWW KNGNEYNEFL YSKAGREELE RVNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05550 Genomic DNA Translation: CAA29065.1 Z49441 Genomic DNA Translation: CAA89461.1 AY558554 Genomic DNA Translation: AAS56880.1 BK006943 Genomic DNA Translation: DAA08637.1 |
PIRi | S48138 |
RefSeqi | NP_012369.1, NM_001181599.1 |
Genome annotation databases
EnsemblFungii | YJL166W_mRNA; YJL166W; YJL166W |
GeneIDi | 853273 |
KEGGi | sce:YJL166W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05550 Genomic DNA Translation: CAA29065.1 Z49441 Genomic DNA Translation: CAA89461.1 AY558554 Genomic DNA Translation: AAS56880.1 BK006943 Genomic DNA Translation: DAA08637.1 |
PIRi | S48138 |
RefSeqi | NP_012369.1, NM_001181599.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EZV | X-ray | 2.30 | G | 2-94 | [»] | |
1KB9 | X-ray | 2.30 | H | 2-94 | [»] | |
1KYO | X-ray | 2.97 | H/S | 2-94 | [»] | |
1P84 | X-ray | 2.50 | H | 2-94 | [»] | |
2IBZ | X-ray | 2.30 | G | 1-94 | [»] | |
3CX5 | X-ray | 1.90 | H/S | 2-94 | [»] | |
3CXH | X-ray | 2.50 | H/S | 2-94 | [»] | |
4PD4 | X-ray | 3.04 | H | 2-94 | [»] | |
6GIQ | electron microscopy | 3.23 | H/S | 1-94 | [»] | |
6HU9 | electron microscopy | 3.35 | H/S | 2-94 | [»] | |
6T0B | electron microscopy | 2.80 | H/S | 1-94 | [»] | |
6T15 | electron microscopy | 3.29 | H/S | 1-94 | [»] | |
6YMX | electron microscopy | 3.17 | H/S | 2-94 | [»] | |
AlphaFoldDBi | P08525 | |||||
SMRi | P08525 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33593, 319 interactors |
ComplexPortali | CPX-567, Mitochondrial respiratory chain complex III |
DIPi | DIP-4706N |
IntActi | P08525, 5 interactors |
MINTi | P08525 |
STRINGi | 4932.YJL166W |
Proteomic databases
MaxQBi | P08525 |
PaxDbi | P08525 |
PRIDEi | P08525 |
TopDownProteomicsi | P08525 |
Genome annotation databases
EnsemblFungii | YJL166W_mRNA; YJL166W; YJL166W |
GeneIDi | 853273 |
KEGGi | sce:YJL166W |
Organism-specific databases
SGDi | S000003702, QCR8 |
VEuPathDBi | FungiDB:YJL166W |
Phylogenomic databases
eggNOGi | KOG4116, Eukaryota |
GeneTreei | ENSGT00390000004029 |
HOGENOMi | CLU_156007_0_1_1 |
InParanoidi | P08525 |
OMAi | AAIFNTW |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P08525 |
PROi | PR:P08525 |
RNActi | P08525, protein |
Family and domain databases
Gene3Di | 1.20.5.210, 1 hit |
InterProi | View protein in InterPro IPR004205, Cyt_bc1_su8 IPR036642, Cyt_bc1_su8_sf |
PANTHERi | PTHR12119, PTHR12119, 1 hit |
Pfami | View protein in Pfam PF02939, UcrQ, 1 hit |
SUPFAMi | SSF81508, SSF81508, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | QCR8_YEAST | |
Accessioni | P08525Primary (citable) accession number: P08525 Secondary accession number(s): D6VW21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families