UniProtKB - P08518 (RPB2_YEAST)
Protein
DNA-directed RNA polymerase II subunit RPB2
Gene
RPB2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. During a transcription cycle, Pol II, general transcription factors and the Mediator complex assemble as the preinitiation complex (PIC) at the promoter. 11-15 base pairs of DNA surrounding the transcription start site are melted and the single-stranded DNA template strand of the promoter is positioned deeply within the central active site cleft of Pol II to form the open complex. After synthesis of about 30 bases of RNA, Pol II releases its contacts with the core promoter and the rest of the transcription machinery (promoter clearance) and enters the stage of transcription elongation in which it moves on the template as the transcript elongates. Pol II appears to oscillate between inactive and active conformations at each step of nucleotide addition. Pol II is composed of mobile elements that move relative to each other. The core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center. The clamp element (portions of RPB1, RPB2 and RPB3) is connected to the core through a set of flexible switches and moves to open and close the cleft. The cleft is surrounded by jaws: an upper jaw formed by portions of RBP1, RPB2 and RPB9, and a lower jaw. The jaws are thought to grab the incoming DNA template. The fork loop 1 (RPB2) interacts with the RNA-DNA hybrid, possibly stabilizing it.
Miscellaneous
The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits.
Present with 18700 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.7.7.6
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 837 | Magnesium; shared with RPB1 | 1 | |
Metal bindingi | 1163 | Zinc | 1 | |
Metal bindingi | 1166 | Zinc | 1 | |
Metal bindingi | 1182 | Zinc | 1 | |
Metal bindingi | 1185 | Zinc | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1163 – 1185 | C4-typeAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: SGD
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: SGD
- ribonucleoside binding Source: InterPro
GO - Biological processi
- transcription, RNA-templated Source: GOC
- transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Transcription |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6781823, Formation of TC-NER Pre-Incision Complex R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-72086, mRNA Capping R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-75955, RNA Polymerase II Transcription Elongation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-77075, RNA Pol II CTD phosphorylation and interaction with CE R-SCE-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase II subunit RPB2 (EC:2.7.7.6)Short name: RNA polymerase II subunit 2 Alternative name(s): B150 DNA-directed RNA polymerase II 140 kDa polypeptide |
Gene namesi | Name:RPB2 Synonyms:RPB150, RPO22 Ordered Locus Names:YOR151C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR151C |
SGDi | S000005677, RPB2 |
Subcellular locationi
Nucleus
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
- RNA polymerase II, core complex Source: SGD
Other locations
- cytoplasmic stress granule Source: SGD
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048091 | 1 – 1224 | DNA-directed RNA polymerase II subunit RPB2Add BLAST | 1224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 919 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P08518 |
PaxDbi | P08518 |
PRIDEi | P08518 |
PTM databases
iPTMneti | P08518 |
Interactioni
Subunit structurei
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.
3 PublicationsBinary interactionsi
Hide detailsP08518
Protein-protein interaction databases
BioGRIDi | 34547, 470 interactors |
ComplexPortali | CPX-2662, DNA-directed RNA polymerase II complex |
DIPi | DIP-14N |
IntActi | P08518, 95 interactors |
MINTi | P08518 |
STRINGi | 4932.YOR151C |
Miscellaneous databases
RNActi | P08518, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08518 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08518 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 467 – 478 | Fork loop 1Add BLAST | 12 |
Sequence similaritiesi
Belongs to the RNA polymerase beta chain family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1163 – 1185 | C4-typeAdd BLAST | 23 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0214, Eukaryota |
GeneTreei | ENSGT00950000183132 |
HOGENOMi | CLU_000524_5_1_1 |
InParanoidi | P08518 |
OMAi | EIMYNGH |
Family and domain databases
CDDi | cd00653, RNA_pol_B_RPB2, 1 hit |
Gene3Di | 2.40.270.10, 1 hit 2.40.50.150, 1 hit 3.90.1110.10, 1 hit |
InterProi | View protein in InterPro IPR015712, DNA-dir_RNA_pol_su2 IPR007120, DNA-dir_RNAP_su2_dom IPR037033, DNA-dir_RNAP_su2_hyb_sf IPR007121, RNA_pol_bsu_CS IPR007644, RNA_pol_bsu_protrusion IPR007642, RNA_pol_Rpb2_2 IPR037034, RNA_pol_Rpb2_2_sf IPR007645, RNA_pol_Rpb2_3 IPR007646, RNA_pol_Rpb2_4 IPR007647, RNA_pol_Rpb2_5 IPR007641, RNA_pol_Rpb2_7 IPR014724, RNA_pol_RPB2_OB-fold |
PANTHERi | PTHR20856, PTHR20856, 1 hit |
Pfami | View protein in Pfam PF04563, RNA_pol_Rpb2_1, 1 hit PF04561, RNA_pol_Rpb2_2, 1 hit PF04565, RNA_pol_Rpb2_3, 1 hit PF04566, RNA_pol_Rpb2_4, 1 hit PF04567, RNA_pol_Rpb2_5, 1 hit PF00562, RNA_pol_Rpb2_6, 1 hit PF04560, RNA_pol_Rpb2_7, 1 hit |
PROSITEi | View protein in PROSITE PS01166, RNA_POL_BETA, 1 hit |
i Sequence
Sequence statusi: Complete.
P08518-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS
60 70 80 90 100
FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP
110 120 130 140 150
MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE
160 170 180 190 200
LIAEESEDDS ESGKVFIGRL PIMLRSKNCY LSEATESDLY KLKECPFDMG
210 220 230 240 250
GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI RSALEKGSRF
260 270 280 290 300
ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH
310 320 330 340 350
ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ
360 370 380 390 400
YAKDILQKEF LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH
410 420 430 440 450
FGKKRLDLAG PLLAQLFKTL FKKLTKDIFR YMQRTVEEAH DFNMKLAINA
460 470 480 490 500
KTITSGLKYA LATGNWGEQK KAMSSRAGVS QVLNRYTYSS TLSHLRRTNT
510 520 530 540 550
PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS LMSCISVGTD
560 570 580 590 600
PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL
610 620 630 640 650
RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE
660 670 680 690 700
LKVRKGHIAK LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES
710 720 730 740 750
ILIAMQPEDL EPAEANEEND LDVDPAKRIR VSHHATTFTH CEIHPSMILG
760 770 780 790 800
VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV FLTNYNVRMD TMANILYYPQ
810 820 830 840 850
KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL
860 870 880 890 900
FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA
910 920 930 940 950
PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD
960 970 980 990 1000
QVLVTTNQDG LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP
1010 1020 1030 1040 1050
FTAEGIVPDL IINPHAIPSR MTVAHLIECL LSKVAALSGN EGDASPFTDI
1060 1070 1080 1090 1100
TVEGISKLLR EHGYQSRGFE VMYNGHTGKK LMAQIFFGPT YYQRLRHMVD
1110 1120 1130 1140 1150
DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA HGAASFLKER
1160 1170 1180 1190 1200
LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA
1210 1220
KLLFQELMAM NITPRLYTDR SRDF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1003 – 1006 | AEGI → RRRY in AAA68096 (PubMed:3547406).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15693 Genomic DNA Translation: AAA68096.1 U55020 Genomic DNA Translation: AAC49637.1 Z75059 Genomic DNA Translation: CAA99357.1 AF527884 Genomic DNA Translation: AAP57849.1 BK006948 Genomic DNA Translation: DAA10924.1 |
PIRi | A25884 |
RefSeqi | NP_014794.3, NM_001183570.3 |
Genome annotation databases
EnsemblFungii | YOR151C_mRNA; YOR151C; YOR151C |
GeneIDi | 854322 |
KEGGi | sce:YOR151C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15693 Genomic DNA Translation: AAA68096.1 U55020 Genomic DNA Translation: AAC49637.1 Z75059 Genomic DNA Translation: CAA99357.1 AF527884 Genomic DNA Translation: AAP57849.1 BK006948 Genomic DNA Translation: DAA10924.1 |
PIRi | A25884 |
RefSeqi | NP_014794.3, NM_001183570.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I3Q | X-ray | 3.10 | B | 1-1224 | [»] | |
1I50 | X-ray | 2.80 | B | 1-1224 | [»] | |
1I6H | X-ray | 3.30 | B | 1-1224 | [»] | |
1K83 | X-ray | 2.80 | B | 1-1224 | [»] | |
1NIK | X-ray | 4.10 | B | 1-1224 | [»] | |
1NT9 | X-ray | 4.20 | B | 1-1224 | [»] | |
1PQV | X-ray | 3.80 | B | 1-1224 | [»] | |
1R5U | X-ray | 4.50 | B | 1-1224 | [»] | |
1R9S | X-ray | 4.25 | B | 1-1224 | [»] | |
1R9T | X-ray | 3.50 | B | 1-1224 | [»] | |
1SFO | X-ray | 3.61 | B | 1-1224 | [»] | |
1TWA | X-ray | 3.20 | B | 1-1224 | [»] | |
1TWC | X-ray | 3.00 | B | 1-1224 | [»] | |
1TWF | X-ray | 2.30 | B | 1-1224 | [»] | |
1TWG | X-ray | 3.30 | B | 1-1224 | [»] | |
1TWH | X-ray | 3.40 | B | 1-1224 | [»] | |
1WCM | X-ray | 3.80 | B | 1-1224 | [»] | |
1Y1V | X-ray | 3.80 | B | 1-1224 | [»] | |
1Y1W | X-ray | 4.00 | B | 1-1224 | [»] | |
1Y1Y | X-ray | 4.00 | B | 1-1224 | [»] | |
1Y77 | X-ray | 4.50 | B | 1-1224 | [»] | |
2B63 | X-ray | 3.80 | B | 1-1224 | [»] | |
2B8K | X-ray | 4.15 | B | 1-1224 | [»] | |
2E2H | X-ray | 3.95 | B | 1-1224 | [»] | |
2E2I | X-ray | 3.41 | B | 1-1224 | [»] | |
2E2J | X-ray | 3.50 | B | 1-1224 | [»] | |
2JA5 | X-ray | 3.80 | B | 1-1224 | [»] | |
2JA6 | X-ray | 4.00 | B | 1-1224 | [»] | |
2JA7 | X-ray | 3.80 | B/N | 1-1224 | [»] | |
2JA8 | X-ray | 3.80 | B | 1-1224 | [»] | |
2NVQ | X-ray | 2.90 | B | 1-1224 | [»] | |
2NVT | X-ray | 3.36 | B | 1-1224 | [»] | |
2NVX | X-ray | 3.60 | B | 1-1224 | [»] | |
2NVY | X-ray | 3.40 | B | 1-1224 | [»] | |
2NVZ | X-ray | 4.30 | B | 1-1224 | [»] | |
2R7Z | X-ray | 3.80 | B | 1-1224 | [»] | |
2R92 | X-ray | 3.80 | B | 1-1224 | [»] | |
2R93 | X-ray | 4.00 | B | 1-1224 | [»] | |
2VUM | X-ray | 3.40 | B | 1-1224 | [»] | |
2YU9 | X-ray | 3.40 | B | 1-1224 | [»] | |
3CQZ | X-ray | 2.80 | B | 1-1223 | [»] | |
3FKI | X-ray | 3.88 | B | 1-1224 | [»] | |
3GTG | X-ray | 3.78 | B | 1-1224 | [»] | |
3GTJ | X-ray | 3.42 | B | 1-1224 | [»] | |
3GTK | X-ray | 3.80 | B | 1-1224 | [»] | |
3GTL | X-ray | 3.38 | B | 1-1224 | [»] | |
3GTM | X-ray | 3.80 | B | 1-1224 | [»] | |
3GTO | X-ray | 4.00 | B | 1-1224 | [»] | |
3GTP | X-ray | 3.90 | B | 1-1224 | [»] | |
3GTQ | X-ray | 3.80 | B | 1-1224 | [»] | |
3H3V | X-ray | 4.00 | C | 1-1224 | [»] | |
3HOU | X-ray | 3.20 | B/N | 1-1224 | [»] | |
3HOV | X-ray | 3.50 | B | 1-1224 | [»] | |
3HOW | X-ray | 3.60 | B | 1-1224 | [»] | |
3HOX | X-ray | 3.65 | B | 1-1224 | [»] | |
3HOY | X-ray | 3.40 | B | 1-1224 | [»] | |
3HOZ | X-ray | 3.65 | B | 1-1224 | [»] | |
3I4M | X-ray | 3.70 | B | 1-1224 | [»] | |
3I4N | X-ray | 3.90 | B | 1-1224 | [»] | |
3J0K | electron microscopy | 36.00 | B | 1-1224 | [»] | |
3J1N | electron microscopy | 16.00 | B | 1-1224 | [»] | |
3K1F | X-ray | 4.30 | B | 1-1224 | [»] | |
3K7A | X-ray | 3.80 | B | 1-1224 | [»] | |
3M3Y | X-ray | 3.18 | B | 1-1224 | [»] | |
3M4O | X-ray | 3.57 | B | 1-1224 | [»] | |
3PO2 | X-ray | 3.30 | B | 1-1224 | [»] | |
3PO3 | X-ray | 3.30 | B | 1-1224 | [»] | |
3QT1 | X-ray | 4.30 | B | 1-1224 | [»] | |
3RZD | X-ray | 3.30 | B | 1-1224 | [»] | |
3RZO | X-ray | 3.00 | B | 1-1224 | [»] | |
3S14 | X-ray | 2.85 | B | 1-1224 | [»] | |
3S15 | X-ray | 3.30 | B | 1-1224 | [»] | |
3S16 | X-ray | 3.24 | B | 1-1224 | [»] | |
3S17 | X-ray | 3.20 | B | 1-1224 | [»] | |
3S1M | X-ray | 3.13 | B | 1-1224 | [»] | |
3S1N | X-ray | 3.10 | B | 1-1224 | [»] | |
3S1Q | X-ray | 3.30 | B | 1-1224 | [»] | |
3S1R | X-ray | 3.20 | B | 1-1224 | [»] | |
3S2D | X-ray | 3.20 | B | 1-1224 | [»] | |
3S2H | X-ray | 3.30 | B | 1-1224 | [»] | |
4A3B | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3C | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3D | X-ray | 3.40 | B | 1-1224 | [»] | |
4A3E | X-ray | 3.40 | B | 1-1224 | [»] | |
4A3F | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3G | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3I | X-ray | 3.80 | B | 1-1224 | [»] | |
4A3J | X-ray | 3.70 | B | 1-1224 | [»] | |
4A3K | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3L | X-ray | 3.50 | B | 1-1224 | [»] | |
4A3M | X-ray | 3.90 | B | 1-1224 | [»] | |
4A93 | X-ray | 3.40 | B | 1-1224 | [»] | |
4BBR | X-ray | 3.40 | B | 1-1224 | [»] | |
4BBS | X-ray | 3.60 | B | 1-1224 | [»] | |
4BXX | X-ray | 3.28 | B | 1-1224 | [»] | |
4BXZ | X-ray | 4.80 | B | 1-1224 | [»] | |
4BY1 | X-ray | 3.60 | B | 1-1224 | [»] | |
4BY7 | X-ray | 3.15 | B | 1-1224 | [»] | |
4V1M | electron microscopy | 6.60 | B | 1-1224 | [»] | |
4V1N | electron microscopy | 7.80 | B | 1-1224 | [»] | |
4V1O | electron microscopy | 9.70 | B | 1-1224 | [»] | |
4X67 | X-ray | 4.10 | B | 1-1224 | [»] | |
4X6A | X-ray | 3.96 | B | 1-1224 | [»] | |
4Y52 | X-ray | 3.50 | B | 1-1224 | [»] | |
4Y7N | X-ray | 3.30 | B | 1-1224 | [»] | |
5C3E | X-ray | 3.70 | B | 1-1224 | [»] | |
5C44 | X-ray | 3.95 | B | 1-1224 | [»] | |
5C4A | X-ray | 4.20 | B | 1-1224 | [»] | |
5C4J | X-ray | 4.00 | B | 1-1224 | [»] | |
5C4X | X-ray | 4.00 | B | 1-1224 | [»] | |
5FMF | electron microscopy | 6.00 | B | 1-1224 | [»] | |
5FYW | electron microscopy | 4.35 | B | 1-1224 | [»] | |
5FZ5 | electron microscopy | 8.80 | B | 1-1224 | [»] | |
5IP7 | X-ray | 3.52 | B | 2-1224 | [»] | |
5IP9 | X-ray | 3.90 | B | 2-1224 | [»] | |
5OQJ | electron microscopy | 4.70 | B | 1-1224 | [»] | |
5OQM | electron microscopy | 5.80 | B | 1-1224 | [»] | |
5OT2 | X-ray | 3.20 | B | 1-1224 | [»] | |
5SVA | electron microscopy | 15.30 | B | 1-1224 | [»] | |
5U5Q | X-ray | 3.80 | B | 1-1224 | [»] | |
5VVR | electron microscopy | 5.80 | B | 1-1224 | [»] | |
5VVS | electron microscopy | 6.40 | B | 1-1224 | [»] | |
5W4U | X-ray | 3.60 | B | 1-1224 | [»] | |
5W51 | X-ray | 3.40 | B | 1-1224 | [»] | |
6BLO | X-ray | 3.40 | B | 1-1224 | [»] | |
6BLP | X-ray | 3.20 | B | 1-1224 | [»] | |
6BM2 | X-ray | 3.40 | B | 1-1224 | [»] | |
6BM4 | X-ray | 2.95 | B | 1-1224 | [»] | |
6BQF | X-ray | 3.35 | B | 1-1224 | [»] | |
6GYK | electron microscopy | 5.10 | B | 1-1224 | [»] | |
6GYL | electron microscopy | 4.80 | B | 1-1224 | [»] | |
6GYM | electron microscopy | 6.70 | B | 1-1224 | [»] | |
6I84 | electron microscopy | 4.40 | B | 1-1224 | [»] | |
6O6C | electron microscopy | 3.10 | B | 1-1224 | [»] | |
6UPX | X-ray | 3.40 | B | 1-1224 | [»] | |
6UPY | X-ray | 3.40 | B | 1-1224 | [»] | |
6UPZ | X-ray | 3.10 | B | 1-1224 | [»] | |
6UQ0 | X-ray | 3.56 | B | 1-1224 | [»] | |
6UQ1 | X-ray | 3.60 | B | 1-1224 | [»] | |
6UQ2 | X-ray | 3.20 | B | 1-1224 | [»] | |
6UQ3 | X-ray | 3.47 | B | 1-1224 | [»] | |
SMRi | P08518 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34547, 470 interactors |
ComplexPortali | CPX-2662, DNA-directed RNA polymerase II complex |
DIPi | DIP-14N |
IntActi | P08518, 95 interactors |
MINTi | P08518 |
STRINGi | 4932.YOR151C |
PTM databases
iPTMneti | P08518 |
Proteomic databases
MaxQBi | P08518 |
PaxDbi | P08518 |
PRIDEi | P08518 |
Genome annotation databases
EnsemblFungii | YOR151C_mRNA; YOR151C; YOR151C |
GeneIDi | 854322 |
KEGGi | sce:YOR151C |
Organism-specific databases
EuPathDBi | FungiDB:YOR151C |
SGDi | S000005677, RPB2 |
Phylogenomic databases
eggNOGi | KOG0214, Eukaryota |
GeneTreei | ENSGT00950000183132 |
HOGENOMi | CLU_000524_5_1_1 |
InParanoidi | P08518 |
OMAi | EIMYNGH |
Enzyme and pathway databases
Reactomei | R-SCE-113418, Formation of the Early Elongation Complex R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6781823, Formation of TC-NER Pre-Incision Complex R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-6796648, TP53 Regulates Transcription of DNA Repair Genes R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-72086, mRNA Capping R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-75955, RNA Polymerase II Transcription Elongation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-77075, RNA Pol II CTD phosphorylation and interaction with CE R-SCE-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
EvolutionaryTracei | P08518 |
PROi | PR:P08518 |
RNActi | P08518, protein |
Family and domain databases
CDDi | cd00653, RNA_pol_B_RPB2, 1 hit |
Gene3Di | 2.40.270.10, 1 hit 2.40.50.150, 1 hit 3.90.1110.10, 1 hit |
InterProi | View protein in InterPro IPR015712, DNA-dir_RNA_pol_su2 IPR007120, DNA-dir_RNAP_su2_dom IPR037033, DNA-dir_RNAP_su2_hyb_sf IPR007121, RNA_pol_bsu_CS IPR007644, RNA_pol_bsu_protrusion IPR007642, RNA_pol_Rpb2_2 IPR037034, RNA_pol_Rpb2_2_sf IPR007645, RNA_pol_Rpb2_3 IPR007646, RNA_pol_Rpb2_4 IPR007647, RNA_pol_Rpb2_5 IPR007641, RNA_pol_Rpb2_7 IPR014724, RNA_pol_RPB2_OB-fold |
PANTHERi | PTHR20856, PTHR20856, 1 hit |
Pfami | View protein in Pfam PF04563, RNA_pol_Rpb2_1, 1 hit PF04561, RNA_pol_Rpb2_2, 1 hit PF04565, RNA_pol_Rpb2_3, 1 hit PF04566, RNA_pol_Rpb2_4, 1 hit PF04567, RNA_pol_Rpb2_5, 1 hit PF00562, RNA_pol_Rpb2_6, 1 hit PF04560, RNA_pol_Rpb2_7, 1 hit |
PROSITEi | View protein in PROSITE PS01166, RNA_POL_BETA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPB2_YEAST | |
Accessioni | P08518Primary (citable) accession number: P08518 Secondary accession number(s): D6W2K8, Q12738, Q7Z9Y0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 218 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD