UniProtKB - P08506 (DACC_ECOLI)
Protein
D-alanyl-D-alanine carboxypeptidase DacC
Gene
dacC
Organism
Escherichia coli (strain K12)
Status
Functioni
Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 66 | Acyl-ester intermediateBy similarity | 1 | |
Active sitei | 69 | Proton acceptorBy similarity | 1 | |
Active sitei | 132 | By similarity | 1 | |
Binding sitei | 235 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: EcoCyc
- endopeptidase activity Source: GO_Central
- penicillin binding Source: EcoCyc
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: EcoCyc
- response to drug Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10203-MONOMER MetaCyc:EG10203-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | S11.003 |
Names & Taxonomyi
Protein namesi | Recommended name: D-alanyl-D-alanine carboxypeptidase DacC (EC:3.4.16.4)Short name: DD-carboxypeptidase Short name: DD-peptidase Alternative name(s): Penicillin-binding protein 6 Short name: PBP-6 |
Gene namesi | Name:dacC Ordered Locus Names:b0839, JW0823 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Periplasmic side 1 Publication
Note: N-terminus lies in the periplasmic space, targeted there by the C-terminal amphiphilic helix (PMID:3330754).
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB00274, Cefmetazole DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB01332, Ceftizoxime DB09050, Ceftolozane DB01000, Cyclacillin DB00303, Ertapenem |
DrugCentrali | P08506 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000027233 | 28 – 400 | D-alanyl-D-alanine carboxypeptidase DacCAdd BLAST | 373 |
Proteomic databases
jPOSTi | P08506 |
PaxDbi | P08506 |
PRIDEi | P08506 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262825, 228 interactors |
IntActi | P08506, 2 interactors |
MINTi | P08506 |
STRINGi | 511145.b0839 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08506 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08506 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 383 – 400 | Required for inner membrane bindingAdd BLAST | 18 |
Domaini
The C-terminus forms an amphiphilic helix that targets the protein to the periplasmic side of the inner cell membrane.1 Publication
Sequence similaritiesi
Belongs to the peptidase S11 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1686, Bacteria |
HOGENOMi | CLU_027070_8_1_6 |
InParanoidi | P08506 |
PhylomeDBi | P08506 |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P08506-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQYSSLLRG LAAGSAFLFL FAPTAFAAEQ TVEAPSVDAR AWILMDYASG
60 70 80 90 100
KVLAEGNADE KLDPASLTKI MTSYVVGQAL KADKIKLTDM VTVGKDAWAT
110 120 130 140 150
GNPALRGSSV MFLKPGDQVS VADLNKGVII QSGNDACIAL ADYVAGSQES
160 170 180 190 200
FIGLMNGYAK KLGLTNTTFQ TVHGLDAPGQ FSTARDMALL GKALIHDVPE
210 220 230 240 250
EYAIHKEKEF TFNKIRQPNR NRLLWSSNLN VDGMKTGTTA GAGYNLVASA
260 270 280 290 300
TQGDMRLISV VLGAKTDRIR FNESEKLLTW GFRFFETVTP IKPDATFVTQ
310 320 330 340 350
RVWFGDKSEV NLGAGEAGSV TIPRGQLKNL KASYTLTEPQ LTAPLKKGQV
360 370 380 390 400
VGTIDFQLNG KSIEQRPLIV MENVEEGGFF GRVWDFVMMK FHQWFGSWFS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 231 | V → E in CAA29775 (PubMed:3279397).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06480 Genomic DNA Translation: CAA29775.1 U00096 Genomic DNA Translation: AAC73926.1 AP009048 Genomic DNA Translation: BAA35542.1 |
PIRi | G64821 |
RefSeqi | NP_415360.1, NC_000913.3 WP_001300708.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73926; AAC73926; b0839 BAA35542; BAA35542; BAA35542 |
GeneIDi | 57729563 945455 |
KEGGi | ecj:JW0823 eco:b0839 |
PATRICi | fig|1411691.4.peg.1439 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06480 Genomic DNA Translation: CAA29775.1 U00096 Genomic DNA Translation: AAC73926.1 AP009048 Genomic DNA Translation: BAA35542.1 |
PIRi | G64821 |
RefSeqi | NP_415360.1, NC_000913.3 WP_001300708.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3IT9 | X-ray | 2.10 | A/B/C/D | 28-378 | [»] | |
3ITA | X-ray | 1.80 | A/B/C/D | 28-378 | [»] | |
3ITB | X-ray | 1.80 | A/B/C/D | 28-378 | [»] | |
SMRi | P08506 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262825, 228 interactors |
IntActi | P08506, 2 interactors |
MINTi | P08506 |
STRINGi | 511145.b0839 |
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB00274, Cefmetazole DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB01332, Ceftizoxime DB09050, Ceftolozane DB01000, Cyclacillin DB00303, Ertapenem |
DrugCentrali | P08506 |
Protein family/group databases
MEROPSi | S11.003 |
Proteomic databases
jPOSTi | P08506 |
PaxDbi | P08506 |
PRIDEi | P08506 |
Genome annotation databases
EnsemblBacteriai | AAC73926; AAC73926; b0839 BAA35542; BAA35542; BAA35542 |
GeneIDi | 57729563 945455 |
KEGGi | ecj:JW0823 eco:b0839 |
PATRICi | fig|1411691.4.peg.1439 |
Organism-specific databases
EchoBASEi | EB0199 |
Phylogenomic databases
eggNOGi | COG1686, Bacteria |
HOGENOMi | CLU_027070_8_1_6 |
InParanoidi | P08506 |
PhylomeDBi | P08506 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10203-MONOMER MetaCyc:EG10203-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P08506 |
PROi | PR:P08506 |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DACC_ECOLI | |
Accessioni | P08506Primary (citable) accession number: P08506 Secondary accession number(s): P77287 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families