Chrm4

Functionⁱ

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functionⁱ

G protein-coupled acetylcholine receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled serotonin receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
guanyl-nucleotide exchange factor activity
Source: RGD
Inferred from direct assayⁱ
- 12048193
neurotransmitter receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
chemical synaptic transmission
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of locomotion Source: InterPro
response to organic substance
Source: RGD
Inferred from expression patternⁱ
- 16843632

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer

Molecular function

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Muscarinic acetylcholine receptor M4
Gene namesⁱ	Name:Chrm4 Synonyms:Chrm-4
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	2344 Chrm4

RGDⁱ

2344 Chrm4

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 30	ExtracellularBy similarityAdd BLAST	30
Transmembraneⁱ	31 – 53	Helical; Name=1By similarityAdd BLAST	23
Topological domainⁱ	54 – 67	CytoplasmicBy similarityAdd BLAST	14
Transmembraneⁱ	68 – 88	Helical; Name=2By similarityAdd BLAST	21
Topological domainⁱ	89 – 105	ExtracellularBy similarityAdd BLAST	17
Transmembraneⁱ	106 – 127	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	128 – 147	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	148 – 170	Helical; Name=4By similarityAdd BLAST	23
Topological domainⁱ	171 – 192	ExtracellularBy similarityAdd BLAST	22
Transmembraneⁱ	193 – 215	Helical; Name=5By similarityAdd BLAST	23
Topological domainⁱ	216 – 400	CytoplasmicBy similarityAdd BLAST	185
Transmembraneⁱ	401 – 421	Helical; Name=6By similarityAdd BLAST	21
Topological domainⁱ	422 – 435	ExtracellularBy similarityAdd BLAST	14
Transmembraneⁱ	436 – 455	Helical; Name=7By similarityAdd BLAST	20
Topological domainⁱ	456 – 478	CytoplasmicBy similarityAdd BLAST	23

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL317
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	16

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000069039	1 – 478	Muscarinic acetylcholine receptor M4Add BLAST		478

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	8	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	13	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	104 ↔ 184	PROSITE-ProRule annotation
Modified residueⁱ	458	PhosphothreonineSequence analysis	1
Modified residueⁱ	462	PhosphothreonineSequence analysis	1
Modified residueⁱ	476	PhosphothreonineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbⁱ	P08485
PRIDEⁱ	P08485

PTM databases

iPTMnetⁱ	P08485
PhosphoSitePlusⁱ	P08485

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Camk2a	P11275	10	EBI-7946147,EBI-2640645

With

Entry

#Exp.

IntAct

Notes

Camk2a

P11275

10

EBI-7946147,EBI-2640645

GO - Molecular functionⁱ

guanyl-nucleotide exchange factor activity
Source: RGD
Inferred from direct assayⁱ
- 12048193

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	P08485, 1 interactor
Molecular INTeraction database More...MINTⁱ	P08485
STRINGⁱ	10116.ENSRNOP00000023586

Chemistry databases

BindingDBⁱ

P08485

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P08485
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4220 Eukaryota ENOG410YCQR LUCA
HOGENOMⁱ	HOG000231484
HOVERGENⁱ	HBG105720
InParanoidⁱ	P08485
KEGG Orthology (KO) More...KOⁱ	K04132
PhylomeDBⁱ	P08485

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd15298 7tmA_mAChR_M4, 1 hit
InterProⁱ	View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR001432 Musac_Ach_M4_rcpt IPR000995 Musac_Ach_rcpt
PANTHERⁱ	PTHR43914:SF6 PTHR43914:SF6, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00237 GPCRRHODOPSN PR00243 MUSCARINICR PR00541 MUSCRINICM4R
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P08485-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI 
        60         70         80         90        100
LVMLSIKVNR QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG 
       110        120        130        140        150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL 
       160        170        180        190        200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI 
       210        220        230        240        250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK 
       260        270        280        290        300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA 
       310        320        330        340        350
TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN 
       360        370        380        390        400
ECVTAIEIVP ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT 
       410        420        430        440        450
IFAILLAFIL TWTPYNVMVL VNTFCQSCIP ERVWSIGYWL CYVNSTINPA 
       460        470 
CYALCNATFK KTFRHLLLCQ YRNIGTAR

Length:478

Mass (Da):52,921

Last modified:August 1, 1988 - v1

Checksum:ⁱ2551FF5F77B1A56C

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	G3V894	G3V894_RAT		Muscarinic acetylcholine receptor Muscarinic acetylcholine receptor	Chrm4 rCG_26726	478	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M16409 mRNA Translation: AAA40663.1 Sequence problems.
PIRⁱ	C94518 C29514
NCBI Reference Sequences More...RefSeqⁱ	NP_113735.1, NM_031547.1
UniGeneⁱ	Rn.10676

Genome annotation databases

GeneIDⁱ	25111
KEGGⁱ	rno:25111
UCSC genome browser More...UCSCⁱ	RGD:2344 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P08485	Muscarinic acetylcholine receptor M4	)	HUMAN		479	UniRef90_P08485
Muscarinic acetylcholine receptor		MUSPF		490
Muscarinic acetylcholine receptor M4 (Fragment)		NEOVI		156
Muscarinic acetylcholine receptor		SHEEP		480
Muscarinic acetylcholine receptor		wild yak		480
+120

Protein	Similar proteins		Species	Score	Length	Source
P08485	Muscarinic acetylcholine receptor M2	)	HUMAN		466	UniRef50_P08172
Muscarinic acetylcholine receptor M2 (Fragment)		PANTR		440
Muscarinic acetylcholine receptor M2		MOUSE		466
Muscarinic acetylcholine receptor M2		PIG		466
Muscarinic acetylcholine receptor M2		RAT		466
+429

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M16409 mRNA Translation: AAA40663.1 Sequence problems.
PIRⁱ	C94518 C29514
RefSeqⁱ	NP_113735.1, NM_031547.1
UniGeneⁱ	Rn.10676

3D structure databases

ProteinModelPortalⁱ	P08485
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P08485, 1 interactor
MINTⁱ	P08485
STRINGⁱ	10116.ENSRNOP00000023586

Chemistry databases

BindingDBⁱ	P08485
ChEMBLⁱ	CHEMBL317
GuidetoPHARMACOLOGYⁱ	16

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

PTM databases

iPTMnetⁱ	P08485
PhosphoSitePlusⁱ	P08485

Proteomic databases

PaxDbⁱ	P08485
PRIDEⁱ	P08485

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	25111
KEGGⁱ	rno:25111
UCSCⁱ	RGD:2344 rat

Organism-specific databases

CTDⁱ	1132
RGDⁱ	2344 Chrm4

Phylogenomic databases

eggNOGⁱ	KOG4220 Eukaryota ENOG410YCQR LUCA
HOGENOMⁱ	HOG000231484
HOVERGENⁱ	HBG105720
InParanoidⁱ	P08485
KOⁱ	K04132
PhylomeDBⁱ	P08485

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P08485

PROⁱ

PR:P08485

Family and domain databases

CDDⁱ	cd15298 7tmA_mAChR_M4, 1 hit
InterProⁱ	View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR001432 Musac_Ach_M4_rcpt IPR000995 Musac_Ach_rcpt
PANTHERⁱ	PTHR43914:SF6 PTHR43914:SF6, 1 hit
Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00237 GPCRRHODOPSN PR00243 MUSCARINICR PR00541 MUSCRINICM4R
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACM4_RAT
Accessionⁱ	P08485Primary (citable) accession number: P08485
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	August 1, 1988
	Last modified:	November 7, 2018
	This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08485 (ACM4_RAT)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Muscarinic acetylcholine receptor M4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ