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Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:Chrm4
Synonyms:Chrm-4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2344 Chrm4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30ExtracellularBy similarityAdd BLAST30
Transmembranei31 – 53Helical; Name=1By similarityAdd BLAST23
Topological domaini54 – 67CytoplasmicBy similarityAdd BLAST14
Transmembranei68 – 88Helical; Name=2By similarityAdd BLAST21
Topological domaini89 – 105ExtracellularBy similarityAdd BLAST17
Transmembranei106 – 127Helical; Name=3By similarityAdd BLAST22
Topological domaini128 – 147CytoplasmicBy similarityAdd BLAST20
Transmembranei148 – 170Helical; Name=4By similarityAdd BLAST23
Topological domaini171 – 192ExtracellularBy similarityAdd BLAST22
Transmembranei193 – 215Helical; Name=5By similarityAdd BLAST23
Topological domaini216 – 400CytoplasmicBy similarityAdd BLAST185
Transmembranei401 – 421Helical; Name=6By similarityAdd BLAST21
Topological domaini422 – 435ExtracellularBy similarityAdd BLAST14
Transmembranei436 – 455Helical; Name=7By similarityAdd BLAST20
Topological domaini456 – 478CytoplasmicBy similarityAdd BLAST23

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL317
GuidetoPHARMACOLOGYi16

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690391 – 478Muscarinic acetylcholine receptor M4Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi13N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
Modified residuei458PhosphothreonineSequence analysis1
Modified residuei462PhosphothreonineSequence analysis1
Modified residuei476PhosphothreonineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08485
PRIDEiP08485

PTM databases

iPTMnetiP08485
PhosphoSitePlusiP08485

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2aP1127510EBI-7946147,EBI-2640645

GO - Molecular functioni

Protein-protein interaction databases

IntActiP08485, 1 interactor
MINTiP08485
STRINGi10116.ENSRNOP00000023586

Chemistry databases

BindingDBiP08485

Structurei

3D structure databases

ProteinModelPortaliP08485
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP08485
KOiK04132
PhylomeDBiP08485

Family and domain databases

CDDicd15298 7tmA_mAChR_M4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001432 Musac_Ach_M4_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF6 PTHR43914:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00541 MUSCRINICM4R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P08485-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN
360 370 380 390 400
ECVTAIEIVP ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT
410 420 430 440 450
IFAILLAFIL TWTPYNVMVL VNTFCQSCIP ERVWSIGYWL CYVNSTINPA
460 470
CYALCNATFK KTFRHLLLCQ YRNIGTAR
Length:478
Mass (Da):52,921
Last modified:August 1, 1988 - v1
Checksum:i2551FF5F77B1A56C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V894G3V894_RAT
Muscarinic acetylcholine receptor
Chrm4 rCG_26726
478Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16409 mRNA Translation: AAA40663.1 Sequence problems.
PIRiC94518 C29514
RefSeqiNP_113735.1, NM_031547.1
UniGeneiRn.10676

Genome annotation databases

GeneIDi25111
KEGGirno:25111
UCSCiRGD:2344 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16409 mRNA Translation: AAA40663.1 Sequence problems.
PIRiC94518 C29514
RefSeqiNP_113735.1, NM_031547.1
UniGeneiRn.10676

3D structure databases

ProteinModelPortaliP08485
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08485, 1 interactor
MINTiP08485
STRINGi10116.ENSRNOP00000023586

Chemistry databases

BindingDBiP08485
ChEMBLiCHEMBL317
GuidetoPHARMACOLOGYi16

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP08485
PhosphoSitePlusiP08485

Proteomic databases

PaxDbiP08485
PRIDEiP08485

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25111
KEGGirno:25111
UCSCiRGD:2344 rat

Organism-specific databases

CTDi1132
RGDi2344 Chrm4

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP08485
KOiK04132
PhylomeDBiP08485

Miscellaneous databases

PROiPR:P08485

Family and domain databases

CDDicd15298 7tmA_mAChR_M4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001432 Musac_Ach_M4_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF6 PTHR43914:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00541 MUSCRINICM4R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACM4_RAT
AccessioniPrimary (citable) accession number: P08485
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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