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UniProtKB - P08485 (ACM4_RAT)
Protein
Muscarinic acetylcholine receptor M4
Gene
Chrm4
Organism
Rattus norvegicus (Rat)
Status
Functioni
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
GO - Molecular functioni
- G protein-coupled acetylcholine receptor activity Source: RGD
- G protein-coupled serotonin receptor activity Source: GO_Central
- guanyl-nucleotide exchange factor activity Source: RGD
- neurotransmitter receptor activity Source: GO_Central
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: RGD
- chemical synaptic transmission Source: GO_Central
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- regulation of locomotion Source: InterPro
- response to organic substance Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Muscarinic acetylcholine receptor M4 |
Gene namesi | Name:Chrm4 Synonyms:Chrm-4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2344, Chrm4 |
Subcellular locationi
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic density membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
Other locations
- asymmetric synapse Source: RGD
- axon terminus Source: RGD
- cytoplasm Source: RGD
- dendrite Source: GO_Central
- glutamatergic synapse Source: SynGO
- membrane Source: RGD
- neuronal cell body Source: RGD
- postsynaptic density Source: RGD
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | ExtracellularBy similarityAdd BLAST | 30 | |
Transmembranei | 31 – 53 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 54 – 67 | CytoplasmicBy similarityAdd BLAST | 14 | |
Transmembranei | 68 – 88 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 89 – 105 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 106 – 127 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 128 – 147 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 148 – 170 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 171 – 192 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 193 – 215 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 216 – 400 | CytoplasmicBy similarityAdd BLAST | 185 | |
Transmembranei | 401 – 421 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 422 – 435 | ExtracellularBy similarityAdd BLAST | 14 | |
Transmembranei | 436 – 455 | Helical; Name=7By similarityAdd BLAST | 20 | |
Topological domaini | 456 – 478 | CytoplasmicBy similarityAdd BLAST | 23 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069039 | 1 – 478 | Muscarinic acetylcholine receptor M4Add BLAST | 478 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 8 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 13 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 104 ↔ 184 | PROSITE-ProRule annotation | ||
Modified residuei | 458 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 462 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 476 | PhosphothreonineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P08485 |
PTM databases
GlyGeni | P08485, 2 sites |
iPTMneti | P08485 |
PhosphoSitePlusi | P08485 |
Interactioni
Binary interactionsi
P08485
With | #Exp. | IntAct |
---|---|---|
Camk2a [P11275] | 10 | EBI-7946147,EBI-2640645 |
Protein-protein interaction databases
IntActi | P08485, 1 interactor |
MINTi | P08485 |
STRINGi | 10116.ENSRNOP00000023586 |
Chemistry databases
BindingDBi | P08485 |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 249 – 333 | DisorderedSequence analysisAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 274 – 288 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 289 – 333 | Polar residuesSequence analysisAdd BLAST | 45 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4220, Eukaryota |
InParanoidi | P08485 |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P08485 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001432, Musac_Ach_M4_rcpt IPR000995, Musac_Ach_rcpt |
PANTHERi | PTHR24248:SF142, PTHR24248:SF142, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00541, MUSCRINICM4R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P08485-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN
360 370 380 390 400
ECVTAIEIVP ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT
410 420 430 440 450
IFAILLAFIL TWTPYNVMVL VNTFCQSCIP ERVWSIGYWL CYVNSTINPA
460 470
CYALCNATFK KTFRHLLLCQ YRNIGTAR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V894 | G3V894_RAT | Muscarinic acetylcholine receptor | Chrm4 rCG_26726 | 478 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16409 mRNA Translation: AAA40663.1 Sequence problems. |
PIRi | C94518, C29514 |
RefSeqi | NP_113735.1, NM_031547.1 |
Genome annotation databases
GeneIDi | 25111 |
KEGGi | rno:25111 |
UCSCi | RGD:2344, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16409 mRNA Translation: AAA40663.1 Sequence problems. |
PIRi | C94518, C29514 |
RefSeqi | NP_113735.1, NM_031547.1 |
3D structure databases
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P08485, 1 interactor |
MINTi | P08485 |
STRINGi | 10116.ENSRNOP00000023586 |
Chemistry databases
BindingDBi | P08485 |
ChEMBLi | CHEMBL317 |
DrugCentrali | P08485 |
GuidetoPHARMACOLOGYi | 16 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P08485, 2 sites |
iPTMneti | P08485 |
PhosphoSitePlusi | P08485 |
Proteomic databases
PaxDbi | P08485 |
Genome annotation databases
GeneIDi | 25111 |
KEGGi | rno:25111 |
UCSCi | RGD:2344, rat |
Organism-specific databases
CTDi | 1132 |
RGDi | 2344, Chrm4 |
Phylogenomic databases
eggNOGi | KOG4220, Eukaryota |
InParanoidi | P08485 |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P08485 |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P08485 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001432, Musac_Ach_M4_rcpt IPR000995, Musac_Ach_rcpt |
PANTHERi | PTHR24248:SF142, PTHR24248:SF142, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00541, MUSCRINICM4R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACM4_RAT | |
Accessioni | P08485Primary (citable) accession number: P08485 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families