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UniProtKB - P08483 (ACM3_RAT)
Protein
Muscarinic acetylcholine receptor M3
Gene
Chrm3
Organism
Rattus norvegicus (Rat)
Status
Functioni
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
3 PublicationsGO - Molecular functioni
- acetylcholine binding Source: UniProtKB
- G protein-coupled acetylcholine receptor activity Source: UniProtKB
- G protein-coupled acetylcholine receptor binding Source: RGD
- G protein-coupled serotonin receptor activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
- quaternary ammonium group binding Source: RGD
GO - Biological processi
- acetylcholine receptor signaling pathway Source: RGD
- adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
- calcium-mediated signaling Source: RGD
- chemical synaptic transmission Source: GO_Central
- G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- negative regulation of heart rate by acetylcholine Source: RGD
- positive regulation of smooth muscle contraction Source: RGD
- positive regulation of vascular associated smooth muscle contraction Source: RGD
- positive regulation of vasoconstriction Source: RGD
- regulation of ion transmembrane transporter activity Source: RGD
- regulation of vascular associated smooth muscle contraction Source: GO_Central
- response to acetylcholine Source: RGD
- saliva secretion Source: RGD
- smooth muscle contraction Source: RGD
- synaptic transmission, cholinergic Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Muscarinic acetylcholine receptor M3 |
Gene namesi | Name:Chrm3 Synonyms:Chrm-3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2343, Chrm3 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- postsynaptic cell membrane ; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- basal plasma membrane Source: RGD
- basolateral plasma membrane Source: UniProtKB-SubCell
- integral component of plasma membrane Source: UniProtKB
- integral component of postsynaptic density membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
Other locations
- asymmetric synapse Source: RGD
- axon terminus Source: RGD
- dendrite Source: RGD
- glutamatergic synapse Source: SynGO
- membrane Source: RGD
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 66 | Extracellular1 PublicationAdd BLAST | 66 | |
Transmembranei | 67 – 90 | Helical; Name=1Add BLAST | 24 | |
Topological domaini | 91 – 103 | Cytoplasmic1 PublicationAdd BLAST | 13 | |
Transmembranei | 104 – 129 | Helical; Name=2Add BLAST | 26 | |
Topological domaini | 130 – 141 | Extracellular1 PublicationAdd BLAST | 12 | |
Transmembranei | 142 – 163 | Helical; Name=3Add BLAST | 22 | |
Topological domaini | 164 – 183 | Cytoplasmic1 PublicationAdd BLAST | 20 | |
Transmembranei | 184 – 205 | Helical; Name=4Add BLAST | 22 | |
Topological domaini | 206 – 228 | Extracellular1 PublicationAdd BLAST | 23 | |
Transmembranei | 229 – 251 | Helical; Name=5Add BLAST | 23 | |
Topological domaini | 252 – 490 | Cytoplasmic1 PublicationAdd BLAST | 239 | |
Transmembranei | 491 – 513 | Helical; Name=6Add BLAST | 23 | |
Topological domaini | 514 – 525 | Extracellular1 PublicationAdd BLAST | 12 | |
Transmembranei | 526 – 545 | Helical; Name=7Add BLAST | 20 | |
Topological domaini | 546 – 589 | Cytoplasmic1 PublicationAdd BLAST | 44 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 148 | Y → A: Decreased affinity for acetylcholine. 1 Publication | 1 | |
Mutagenesisi | 231 | T → A: Decreased affinity for acetylcholine. 1 Publication | 1 | |
Mutagenesisi | 234 | T → A: Strongly decreased affinity for acetylcholine. 2 Publications | 1 | |
Mutagenesisi | 506 | Y → F: Decreased affinity for acetylcholine. 2 Publications | 1 | |
Mutagenesisi | 529 | Y → F: Decreased affinity for acetylcholine. 1 Publication | 1 | |
Mutagenesisi | 533 | Y → F: Decreased affinity for acetylcholine. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL320 |
DrugCentrali | P08483 |
GuidetoPHARMACOLOGYi | 15 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069034 | 1 – 589 | Muscarinic acetylcholine receptor M3Add BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 52 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 140 ↔ 220 | PROSITE-ProRule annotation1 Publication | ||
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 516 ↔ 519 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P08483 |
PTM databases
GlyGeni | P08483, 5 sites |
PhosphoSitePlusi | P08483 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000049410, Expressed in frontal cortex and 18 other tissues |
Genevisiblei | P08483, RN |
Interactioni
Subunit structurei
Homodimer; the dimers can form tetramers (By similarity).
Interacts with NALCN (By similarity).
Interacts with TMEM147 (By similarity).
By similarityBinary interactionsi
P08483
With | #Exp. | IntAct |
---|---|---|
Camk2a [P11275] | 2 | EBI-7946407,EBI-2640645 |
GO - Molecular functioni
- G protein-coupled acetylcholine receptor binding Source: RGD
Protein-protein interaction databases
IntActi | P08483, 1 interactor |
MINTi | P08483 |
STRINGi | 10116.ENSRNOP00000067435 |
Chemistry databases
BindingDBi | P08483 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08483 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 147 – 151 | Agonist bindingCurated | 5 | |
Regioni | 323 – 356 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 506 – 529 | Agonist bindingCuratedAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 274 – 280 | Basolateral sorting signalBy similarity | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 323 – 351 | Polar residuesSequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4220, Eukaryota |
GeneTreei | ENSGT00940000160084 |
HOGENOMi | CLU_009579_11_2_1 |
InParanoidi | P08483 |
OMAi | PPDECFI |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P08483 |
TreeFami | TF320495 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001183, Musac_Ach_M3_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00540, MUSCRINICM3R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P08483-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVTQLGSY NISQETGNFS
60 70 80 90 100
SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT
110 120 130 140 150
VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA
160 170 180 190 200
SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA
210 220 230 240 250
PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY
260 270 280 290 300
WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGVK
310 320 330 340 350
RSSRRKYGRC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS
360 370 380 390 400
SDEEDIGSET RAIYSIVLKL PGHSSILNST KLPSSDNLQV SNEDLGTVDV
410 420 430 440 450
ERNAHKLQAQ KSMGDGDNCQ KDFTKLPIQL ESAVDTGKTS DTNSSADKTT
460 470 480 490 500
ATLPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI
510 520 530 540 550
ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF
560 570 580
RTTFKTLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | A → R in AAA40661 (PubMed:3037705).Curated | 1 | |
Sequence conflicti | 184 | A → R in AAA40662 (PubMed:3037705).Curated | 1 | |
Sequence conflicti | 184 | A → R in BAA36839 (PubMed:9972520).Curated | 1 | |
Sequence conflicti | 516 | C → R in AAA40659 (PubMed:3120722).Curated | 1 | |
Sequence conflicti | 556 | T → M in AAA40659 (PubMed:3120722).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16407 mRNA Translation: AAA40661.1 M16408 Genomic DNA Translation: AAA40662.1 M18088 mRNA Translation: AAA40659.1 M62826 Genomic DNA Translation: AAA41553.1 AB017656 mRNA Translation: BAA36839.1 |
PIRi | A29476 B94518, B29514 |
RefSeqi | NP_036659.2, NM_012527.2 XP_017455938.1, XM_017600449.1 XP_017455939.1, XM_017600450.1 XP_017455940.1, XM_017600451.1 XP_017455941.1, XM_017600452.1 |
Genome annotation databases
Ensembli | ENSRNOT00000075651; ENSRNOP00000067435; ENSRNOG00000049410 |
GeneIDi | 24260 |
KEGGi | rno:24260 |
UCSCi | RGD:2343, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16407 mRNA Translation: AAA40661.1 M16408 Genomic DNA Translation: AAA40662.1 M18088 mRNA Translation: AAA40659.1 M62826 Genomic DNA Translation: AAA41553.1 AB017656 mRNA Translation: BAA36839.1 |
PIRi | A29476 B94518, B29514 |
RefSeqi | NP_036659.2, NM_012527.2 XP_017455938.1, XM_017600449.1 XP_017455939.1, XM_017600450.1 XP_017455940.1, XM_017600451.1 XP_017455941.1, XM_017600452.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DAJ | X-ray | 3.40 | A/B/C/D | 57-259 | [»] | |
A/B/C/D | 482-589 | [»] | ||||
4U14 | X-ray | 3.57 | A | 57-259 | [»] | |
A | 482-563 | [»] | ||||
4U15 | X-ray | 2.80 | A/B | 57-259 | [»] | |
A/B | 482-563 | [»] | ||||
4U16 | X-ray | 3.70 | A/B | 57-259 | [»] | |
A/B | 482-563 | [»] | ||||
5ZHP | X-ray | 3.10 | A/B | 57-308 | [»] | |
A/B | 482-569 | [»] | ||||
SMRi | P08483 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P08483, 1 interactor |
MINTi | P08483 |
STRINGi | 10116.ENSRNOP00000067435 |
Chemistry databases
BindingDBi | P08483 |
ChEMBLi | CHEMBL320 |
DrugCentrali | P08483 |
GuidetoPHARMACOLOGYi | 15 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P08483, 5 sites |
PhosphoSitePlusi | P08483 |
Proteomic databases
PaxDbi | P08483 |
Genome annotation databases
Ensembli | ENSRNOT00000075651; ENSRNOP00000067435; ENSRNOG00000049410 |
GeneIDi | 24260 |
KEGGi | rno:24260 |
UCSCi | RGD:2343, rat |
Organism-specific databases
CTDi | 1131 |
RGDi | 2343, Chrm3 |
Phylogenomic databases
eggNOGi | KOG4220, Eukaryota |
GeneTreei | ENSGT00940000160084 |
HOGENOMi | CLU_009579_11_2_1 |
InParanoidi | P08483 |
OMAi | PPDECFI |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P08483 |
TreeFami | TF320495 |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-416476, G alpha (q) signalling events |
Miscellaneous databases
PROi | PR:P08483 |
Gene expression databases
Bgeei | ENSRNOG00000049410, Expressed in frontal cortex and 18 other tissues |
Genevisiblei | P08483, RN |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001183, Musac_Ach_M3_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00540, MUSCRINICM3R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACM3_RAT | |
Accessioni | P08483Primary (citable) accession number: P08483 Secondary accession number(s): Q9QWK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families