UniProtKB - P08473 (NEP_HUMAN)
Protein
Neprilysin
Gene
MME
Organism
Homo sapiens (Human)
Status
Functioni
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:2531377, PubMed:2972276, PubMed:16254193). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).6 Publications
Miscellaneous
Important cell surface marker in the diagnostic of human acute lymphocytic leukemia.
Catalytic activityi
- Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.3 Publications EC:3.4.24.11
Cofactori
Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications
Activity regulationi
Inhibited in a dose dependent manner by opiorphin (PubMed:17101991). Activated by K49-P1-20, a twenty-residue synthetic peptide shortened from the snake B.asper myotoxin II (PubMed:26931059).2 Publications
Kineticsi
- KM=55.1 µM for angiotensin-11 Publication
- KM=179 µM for angiotensin-21 Publication
- KM=111.4 µM for angiotensin 1-91 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 103 | Substrate carboxylBy similarity | 1 | |
Metal bindingi | 584 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 585 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 588 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 647 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 651 | Proton donorPROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- cardiolipin binding Source: ARUK-UCL
- endopeptidase activity Source: UniProtKB
- exopeptidase activity Source: BHF-UCL
- metalloendopeptidase activity Source: UniProtKB
- metallopeptidase activity Source: Reactome
- oligopeptidase activity Source: Ensembl
- peptide binding Source: UniProtKB
- phosphatidylserine binding Source: ARUK-UCL
- protein homodimerization activity Source: ARUK-UCL
- zinc ion binding Source: UniProtKB
GO - Biological processi
- aging Source: ARUK-UCL
- amyloid-beta clearance Source: ARUK-UCL
- amyloid-beta clearance by cellular catabolic process Source: ARUK-UCL
- amyloid-beta metabolic process Source: UniProtKB
- angiotensin maturation Source: Reactome
- cellular response to cytokine stimulus Source: UniProtKB
- cellular response to UV-A Source: UniProtKB
- cellular response to UV-B Source: UniProtKB
- creatinine metabolic process Source: UniProtKB
- kidney development Source: UniProtKB
- learning or memory Source: ARUK-UCL
- lung development Source: Ensembl
- neuropeptide processing Source: ARUK-UCL
- neutrophil degranulation Source: Reactome
- peptide metabolic process Source: UniProtKB
- placenta development Source: Ensembl
- positive regulation of long-term synaptic potentiation Source: Ensembl
- positive regulation of neurogenesis Source: ARUK-UCL
- protein processing Source: GO_Central
- proteolysis Source: UniProtKB
- replicative senescence Source: UniProtKB
- sensory perception of pain Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.11, 2681 |
PathwayCommonsi | P08473 |
Reactomei | R-HSA-2022377, Metabolism of Angiotensinogen to Angiotensins R-HSA-6798695, Neutrophil degranulation |
SIGNORi | P08473 |
Protein family/group databases
MEROPSi | M13.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Neprilysin (EC:3.4.24.113 Publications)Alternative name(s): Atriopeptidase Common acute lymphocytic leukemia antigen Short name: CALLA Enkephalinase Neutral endopeptidase 24.11 Short name: NEP Short name: Neutral endopeptidase Skin fibroblast elastase Short name: SFE CD_antigen: CD10 |
Gene namesi | Name:MME Synonyms:EPN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7154, MME |
MIMi | 120520, gene |
neXtProti | NX_P08473 |
VEuPathDBi | HostDB:ENSG00000196549.10 |
Subcellular locationi
Plasma membrane
Endosome
- early endosome Source: ARUK-UCL
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- trans-Golgi network Source: ARUK-UCL
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- axon Source: ARUK-UCL
- brush border Source: UniProtKB
- cell surface Source: ARUK-UCL
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: ARUK-UCL
- dendrite Source: UniProtKB
- focal adhesion Source: UniProtKB
- integral component of membrane Source: UniProtKB
- membrane raft Source: ARUK-UCL
- neuron projection terminus Source: UniProtKB
- neuronal cell body Source: ARUK-UCL
- presynapse Source: ARUK-UCL
- secretory granule membrane Source: Reactome
- synapse Source: UniProtKB
- synaptic vesicle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 28 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 29 – 51 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 52 – 750 | ExtracellularSequence analysisAdd BLAST | 699 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Charcot-Marie-Tooth disease 2T (CMT2T)2 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_077684 | 12 | D → A in CMT2T; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077686 | 347 | Y → C in CMT2T; results in reduction of neprilysin activity. 1 PublicationCorresponds to variant dbSNP:rs138218277EnsemblClinVar. | 1 | |
Natural variantiVAR_077687 | 348 | A → P in CMT2T; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs199567914Ensembl. | 1 | |
Natural variantiVAR_077688 | 411 | Missing in CMT2T; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077689 | 422 | A → D in CMT2T; late-onset form; results in reduction of neprilysin activity. 1 PublicationCorresponds to variant dbSNP:rs777476150EnsemblClinVar. | 1 | |
Natural variantiVAR_077691 | 621 | C → R in CMT2T; decrease of protein expression. 1 PublicationCorresponds to variant dbSNP:rs879253752EnsemblClinVar. | 1 |
Spinocerebellar ataxia 43 (SCA43)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA43 is a slowly progressive, autosomal dominant form.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_077685 | 143 | C → Y in SCA43. 1 PublicationCorresponds to variant dbSNP:rs879255651EnsemblClinVar. | 1 |
Keywords - Diseasei
Charcot-Marie-Tooth disease, Disease variant, Neurodegeneration, Neuropathy, Spinocerebellar ataxiaOrganism-specific databases
DisGeNETi | 4311 |
GeneReviewsi | MME |
MalaCardsi | MME |
MIMi | 617017, phenotype 617018, phenotype |
OpenTargetsi | ENSG00000196549 |
Orphaneti | 495274, Charcot-Marie-Tooth disease type 2T 69063, Congenital membranous nephropathy due to fetomaternal anti-neutral endopeptidase alloimmunization 497757, MME-related autosomal dominant Charcot Marie Tooth disease type 2 497764, Spinocerebellar ataxia type 43 |
PharmGKBi | PA30864 |
Miscellaneous databases
Pharosi | P08473, Tclin |
Chemistry databases
ChEMBLi | CHEMBL1944 |
DrugBanki | DB08575, 2-[(1S)-1-BENZYL-2-SULFANYLETHYL]-1H-IMIDAZO[4,5-C]PYRIDIN-5-IUM DB02597, [2(R,S)-2-Sulfanylheptanoyl]-Phe-Ala DB00616, Candoxatril DB11623, Candoxatrilat DB05796, Daglutril DB06655, Liraglutide DB02558, N-(3-Phenyl-2-Sulfanylpropanoyl)Phenylalanylalanine DB02062, N-[3-[(1-Aminoethyl)(Hydroxy)Phosphoryl]-2-(1,1'-Biphenyl-4-Ylmethyl)Propanoyl]Alanine DB00886, Omapatrilat DB02557, Phosphoramidon DB09292, Sacubitril DB13928, Semaglutide DB08626, Thiorphan |
DrugCentrali | P08473 |
GuidetoPHARMACOLOGYi | 1611 |
Genetic variation databases
BioMutai | MME |
DMDMi | 128062 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000078213 | 2 – 750 | NeprilysinAdd BLAST | 749 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 4 | PhosphoserineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 57 ↔ 62 | PROSITE-ProRule annotation | ||
Disulfide bondi | 80 ↔ 735 | PROSITE-ProRule annotation | ||
Disulfide bondi | 88 ↔ 695 | PROSITE-ProRule annotation | ||
Disulfide bondi | 143 ↔ 411 | PROSITE-ProRule annotation | ||
Glycosylationi | 145 | N-linked (GlcNAc...) asparagine4 Publications | 1 | |
Disulfide bondi | 234 ↔ 242 | PROSITE-ProRule annotation | ||
Glycosylationi | 285 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 325 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Disulfide bondi | 621 ↔ 747 | PROSITE-ProRule annotation | ||
Glycosylationi | 628 | N-linked (GlcNAc...) asparagine4 Publications | 1 |
Post-translational modificationi
Myristoylation is a determinant of membrane targeting.1 Publication
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.5 Publications
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | P08473 |
jPOSTi | P08473 |
MassIVEi | P08473 |
MaxQBi | P08473 |
PaxDbi | P08473 |
PeptideAtlasi | P08473 |
PRIDEi | P08473 |
ProteomicsDBi | 52110 |
PTM databases
CarbonylDBi | P08473 |
GlyConnecti | 1541, 1 N-Linked glycan (1 site) |
GlyGeni | P08473, 6 sites |
iPTMneti | P08473 |
PhosphoSitePlusi | P08473 |
Expressioni
Gene expression databases
Bgeei | ENSG00000196549, Expressed in metanephric glomerulus and 174 other tissues |
ExpressionAtlasi | P08473, baseline and differential |
Genevisiblei | P08473, HS |
Organism-specific databases
HPAi | ENSG00000196549, Tissue enhanced (blood, intestine, kidney) |
Interactioni
Binary interactionsi
Hide detailsP08473
GO - Molecular functioni
- protein homodimerization activity Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 110455, 118 interactors |
IntActi | P08473, 104 interactors |
MINTi | P08473 |
STRINGi | 9606.ENSP00000418525 |
Chemistry databases
BindingDBi | P08473 |
Miscellaneous databases
RNActi | P08473, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P08473 |
SMRi | P08473 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08473 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 750 | Peptidase M13PROSITE-ProRule annotationAdd BLAST | 695 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 16 – 23 | Stop-transfer sequenceSequence analysis | 8 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3624, Eukaryota |
GeneTreei | ENSGT00940000156745 |
HOGENOMi | CLU_006187_8_0_1 |
InParanoidi | P08473 |
OMAi | APRNHDA |
OrthoDBi | 282463at2759 |
PhylomeDBi | P08473 |
TreeFami | TF315192 |
Family and domain databases
CDDi | cd08662, M13, 1 hit |
Gene3Di | 1.10.1380.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR024079, MetalloPept_cat_dom_sf IPR029727, MME/CD10/NEP IPR000718, Peptidase_M13 IPR018497, Peptidase_M13_C IPR042089, Peptidase_M13_dom_2 IPR008753, Peptidase_M13_N |
PANTHERi | PTHR11733, PTHR11733, 1 hit PTHR11733:SF114, PTHR11733:SF114, 1 hit |
Pfami | View protein in Pfam PF01431, Peptidase_M13, 1 hit PF05649, Peptidase_M13_N, 1 hit |
PRINTSi | PR00786, NEPRILYSIN |
PROSITEi | View protein in PROSITE PS51885, NEPRILYSIN, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All
P08473-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKSESQMDI TDINTPKPKK KQRWTPLEIS LSVLVLLLTI IAVTMIALYA
60 70 80 90 100
TYDDGICKSS DCIKSAARLI QNMDATTEPC TDFFKYACGG WLKRNVIPET
110 120 130 140 150
SSRYGNFDIL RDELEVVLKD VLQEPKTEDI VAVQKAKALY RSCINESAID
160 170 180 190 200
SRGGEPLLKL LPDIYGWPVA TENWEQKYGA SWTAEKAIAQ LNSKYGKKVL
210 220 230 240 250
INLFVGTDDK NSVNHVIHID QPRLGLPSRD YYECTGIYKE ACTAYVDFMI
260 270 280 290 300
SVARLIRQEE RLPIDENQLA LEMNKVMELE KEIANATAKP EDRNDPMLLY
310 320 330 340 350
NKMTLAQIQN NFSLEINGKP FSWLNFTNEI MSTVNISITN EEDVVVYAPE
360 370 380 390 400
YLTKLKPILT KYSARDLQNL MSWRFIMDLV SSLSRTYKES RNAFRKALYG
410 420 430 440 450
TTSETATWRR CANYVNGNME NAVGRLYVEA AFAGESKHVV EDLIAQIREV
460 470 480 490 500
FIQTLDDLTW MDAETKKRAE EKALAIKERI GYPDDIVSND NKLNNEYLEL
510 520 530 540 550
NYKEDEYFEN IIQNLKFSQS KQLKKLREKV DKDEWISGAA VVNAFYSSGR
560 570 580 590 600
NQIVFPAGIL QPPFFSAQQS NSLNYGGIGM VIGHEITHGF DDNGRNFNKD
610 620 630 640 650
GDLVDWWTQQ SASNFKEQSQ CMVYQYGNFS WDLAGGQHLN GINTLGENIA
660 670 680 690 700
DNGGLGQAYR AYQNYIKKNG EEKLLPGLDL NHKQLFFLNF AQVWCGTYRP
710 720 730 740 750
EYAVNSIKTD VHSPGNFRII GTLQNSAEFS EAFHCRKNSY MNPEKKCRVW
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A6Q8PFY6 | A0A6Q8PFY6_HUMAN | Atriopeptidase | MME | 703 | Annotation score: | ||
C9J9X7 | C9J9X7_HUMAN | Atriopeptidase | MME | 161 | Annotation score: | ||
C9IYX7 | C9IYX7_HUMAN | Atriopeptidase | MME | 119 | Annotation score: | ||
C9JDZ3 | C9JDZ3_HUMAN | Neprilysin | MME | 93 | Annotation score: | ||
Q3KQS6 | Q3KQS6_HUMAN | MME protein | MME | 80 | Annotation score: | ||
C9J7X4 | C9J7X4_HUMAN | Neprilysin | MME | 15 | Annotation score: | ||
A0A7I2U302 | A0A7I2U302_HUMAN | Neprilysin | MME | 780 | Annotation score: |
Sequence cautioni
The sequence CAA30157 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | P → R in AAA51915 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 44 | T → R in AAA51915 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 81 | T → R in AAA51915 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 304 | T → R in AAA51915 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_077684 | 12 | D → A in CMT2T; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077685 | 143 | C → Y in SCA43. 1 PublicationCorresponds to variant dbSNP:rs879255651EnsemblClinVar. | 1 | |
Natural variantiVAR_077686 | 347 | Y → C in CMT2T; results in reduction of neprilysin activity. 1 PublicationCorresponds to variant dbSNP:rs138218277EnsemblClinVar. | 1 | |
Natural variantiVAR_077687 | 348 | A → P in CMT2T; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs199567914Ensembl. | 1 | |
Natural variantiVAR_077688 | 411 | Missing in CMT2T; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077689 | 422 | A → D in CMT2T; late-onset form; results in reduction of neprilysin activity. 1 PublicationCorresponds to variant dbSNP:rs777476150EnsemblClinVar. | 1 | |
Natural variantiVAR_077690 | 497 | Y → H1 PublicationCorresponds to variant dbSNP:rs200308207Ensembl. | 1 | |
Natural variantiVAR_077691 | 621 | C → R in CMT2T; decrease of protein expression. 1 PublicationCorresponds to variant dbSNP:rs879253752EnsemblClinVar. | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000360490; ENSP00000353679; ENSG00000196549 ENST00000460393; ENSP00000418525; ENSG00000196549 ENST00000462745; ENSP00000419653; ENSG00000196549 ENST00000492661; ENSP00000420389; ENSG00000196549 ENST00000493237; ENSP00000417079; ENSG00000196549 |
GeneIDi | 4311 |
KEGGi | hsa:4311 |
UCSCi | uc003fab.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DL9 | model | - | A | 508-750 | [»] | |
1DMT | X-ray | 2.10 | A | 55-750 | [»] | |
1QVD | model | - | A | 55-750 | [»] | |
1R1H | X-ray | 1.95 | A | 55-750 | [»] | |
1R1I | X-ray | 2.60 | A | 55-750 | [»] | |
1R1J | X-ray | 2.35 | A | 55-750 | [»] | |
1Y8J | X-ray | 2.25 | A | 55-750 | [»] | |
2QPJ | X-ray | 2.05 | A | 55-750 | [»] | |
2YB9 | X-ray | 2.40 | A | 55-750 | [»] | |
4CTH | X-ray | 2.15 | A | 52-750 | [»] | |
5JMY | X-ray | 2.00 | A/B | 53-750 | [»] | |
6GID | X-ray | 1.90 | A | 55-750 | [»] | |
6SH1 | X-ray | 2.10 | AAA/CCC | 55-750 | [»] | |
6SH2 | X-ray | 2.60 | AAA | 55-750 | [»] | |
6SUK | X-ray | 1.75 | A | 52-750 | [»] | |
6SVY | X-ray | 2.60 | A | 52-750 | [»] | |
6THP | X-ray | 2.54 | A/B | 55-750 | [»] | |
6XVP | X-ray | 2.65 | A | 52-750 | [»] | |
SASBDBi | P08473 | |||||
SMRi | P08473 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110455, 118 interactors |
IntActi | P08473, 104 interactors |
MINTi | P08473 |
STRINGi | 9606.ENSP00000418525 |
Chemistry databases
BindingDBi | P08473 |
ChEMBLi | CHEMBL1944 |
DrugBanki | DB08575, 2-[(1S)-1-BENZYL-2-SULFANYLETHYL]-1H-IMIDAZO[4,5-C]PYRIDIN-5-IUM DB02597, [2(R,S)-2-Sulfanylheptanoyl]-Phe-Ala DB00616, Candoxatril DB11623, Candoxatrilat DB05796, Daglutril DB06655, Liraglutide DB02558, N-(3-Phenyl-2-Sulfanylpropanoyl)Phenylalanylalanine DB02062, N-[3-[(1-Aminoethyl)(Hydroxy)Phosphoryl]-2-(1,1'-Biphenyl-4-Ylmethyl)Propanoyl]Alanine DB00886, Omapatrilat DB02557, Phosphoramidon DB09292, Sacubitril DB13928, Semaglutide DB08626, Thiorphan |
DrugCentrali | P08473 |
GuidetoPHARMACOLOGYi | 1611 |
Protein family/group databases
MEROPSi | M13.001 |
PTM databases
CarbonylDBi | P08473 |
GlyConnecti | 1541, 1 N-Linked glycan (1 site) |
GlyGeni | P08473, 6 sites |
iPTMneti | P08473 |
PhosphoSitePlusi | P08473 |
Genetic variation databases
BioMutai | MME |
DMDMi | 128062 |
Proteomic databases
EPDi | P08473 |
jPOSTi | P08473 |
MassIVEi | P08473 |
MaxQBi | P08473 |
PaxDbi | P08473 |
PeptideAtlasi | P08473 |
PRIDEi | P08473 |
ProteomicsDBi | 52110 |
Protocols and materials databases
Antibodypediai | 3658, 2327 antibodies |
Genome annotation databases
Ensembli | ENST00000360490; ENSP00000353679; ENSG00000196549 ENST00000460393; ENSP00000418525; ENSG00000196549 ENST00000462745; ENSP00000419653; ENSG00000196549 ENST00000492661; ENSP00000420389; ENSG00000196549 ENST00000493237; ENSP00000417079; ENSG00000196549 |
GeneIDi | 4311 |
KEGGi | hsa:4311 |
UCSCi | uc003fab.2, human |
Organism-specific databases
CTDi | 4311 |
DisGeNETi | 4311 |
GeneCardsi | MME |
GeneReviewsi | MME |
HGNCi | HGNC:7154, MME |
HPAi | ENSG00000196549, Tissue enhanced (blood, intestine, kidney) |
MalaCardsi | MME |
MIMi | 120520, gene 617017, phenotype 617018, phenotype |
neXtProti | NX_P08473 |
OpenTargetsi | ENSG00000196549 |
Orphaneti | 495274, Charcot-Marie-Tooth disease type 2T 69063, Congenital membranous nephropathy due to fetomaternal anti-neutral endopeptidase alloimmunization 497757, MME-related autosomal dominant Charcot Marie Tooth disease type 2 497764, Spinocerebellar ataxia type 43 |
PharmGKBi | PA30864 |
VEuPathDBi | HostDB:ENSG00000196549.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3624, Eukaryota |
GeneTreei | ENSGT00940000156745 |
HOGENOMi | CLU_006187_8_0_1 |
InParanoidi | P08473 |
OMAi | APRNHDA |
OrthoDBi | 282463at2759 |
PhylomeDBi | P08473 |
TreeFami | TF315192 |
Enzyme and pathway databases
BRENDAi | 3.4.24.11, 2681 |
PathwayCommonsi | P08473 |
Reactomei | R-HSA-2022377, Metabolism of Angiotensinogen to Angiotensins R-HSA-6798695, Neutrophil degranulation |
SIGNORi | P08473 |
Miscellaneous databases
BioGRID-ORCSi | 4311, 1 hit in 994 CRISPR screens |
ChiTaRSi | MME, human |
EvolutionaryTracei | P08473 |
GeneWikii | Neprilysin |
GenomeRNAii | 4311 |
Pharosi | P08473, Tclin |
PROi | PR:P08473 |
RNActi | P08473, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196549, Expressed in metanephric glomerulus and 174 other tissues |
ExpressionAtlasi | P08473, baseline and differential |
Genevisiblei | P08473, HS |
Family and domain databases
CDDi | cd08662, M13, 1 hit |
Gene3Di | 1.10.1380.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR024079, MetalloPept_cat_dom_sf IPR029727, MME/CD10/NEP IPR000718, Peptidase_M13 IPR018497, Peptidase_M13_C IPR042089, Peptidase_M13_dom_2 IPR008753, Peptidase_M13_N |
PANTHERi | PTHR11733, PTHR11733, 1 hit PTHR11733:SF114, PTHR11733:SF114, 1 hit |
Pfami | View protein in Pfam PF01431, Peptidase_M13, 1 hit PF05649, Peptidase_M13_N, 1 hit |
PRINTSi | PR00786, NEPRILYSIN |
PROSITEi | View protein in PROSITE PS51885, NEPRILYSIN, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEP_HUMAN | |
Accessioni | P08473Primary (citable) accession number: P08473 Secondary accession number(s): A8K6U6, D3DNJ9, Q3MIX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 236 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Peptidase families
Classification of peptidase families and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families