UniProtKB - P08461 (ODP2_RAT)
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Dlat
Functioni
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Catalytic activityi
- (R)-N6-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-N6-(S8-acetyldihydrolipoyl)-L-lysyl-[protein] + CoAEC:2.3.1.12
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 605 | Sequence analysis | 1 | |
Active sitei | 609 | Sequence analysis | 1 |
GO - Molecular functioni
- dihydrolipoyllysine-residue acetyltransferase activity Source: RGD
- identical protein binding Source: RGD
- nucleotide binding Source: RGD
GO - Biological processi
- acetyl-CoA biosynthetic process from pyruvate Source: RGD
- glucose metabolic process Source: UniProtKB-KW
- sleep Source: RGD
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle |
Enzyme and pathway databases
Reactomei | R-RNO-204174, Regulation of pyruvate dehydrogenase (PDH) complex R-RNO-389661, Glyoxylate metabolism and glycine degradation R-RNO-5362517, Signaling by Retinoic Acid R-RNO-70268, Pyruvate metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)Alternative name(s): 70 kDa mitochondrial autoantigen of primary biliary cirrhosis Short name: PBC Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex Pyruvate dehydrogenase complex component E2 Short name: PDC-E2 Short name: PDCE2 |
Gene namesi | Name:Dlat |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619859, Dlat |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial pyruvate dehydrogenase complex Source: RGD
- mitochondrion Source: RGD
Other locations
- pyruvate dehydrogenase complex Source: RGD
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Involvement in diseasei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 77 | Mitochondrion1 PublicationAdd BLAST | 77 | |
ChainiPRO_0000162298 | 78 – 632 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrialAdd BLAST | 555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Modified residuei | 123 | N6-lipoyllysinePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 249 | N6-lipoyllysinePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 451 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 458 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 532 | N6-succinyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P08461 |
PaxDbi | P08461 |
PRIDEi | P08461 |
2D gel databases
World-2DPAGEi | 0004:P08461 |
PTM databases
iPTMneti | P08461 |
PhosphoSitePlusi | P08461 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000009994, Expressed in heart and 20 other tissues |
Interactioni
Subunit structurei
Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (subunits PDH1A and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.
Interacts with PDK2 and PDK3.
Interacts with SIRT4.
Interacts with PDHB.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249554, 4 interactors |
IntActi | P08461, 4 interactors |
MINTi | P08461 |
STRINGi | 10116.ENSRNOP00000032890 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 158 | Lipoyl-binding 1PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 208 – 284 | Lipoyl-binding 2PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 342 – 379 | Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST | 38 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 456 – 632 | CatalyticBy similarityAdd BLAST | 177 | |
Regioni | 600 – 611 | CoA-bindingBy similarityAdd BLAST | 12 |
Sequence similaritiesi
Keywords - Domaini
Lipoyl, Repeat, Transit peptidePhylogenomic databases
eggNOGi | KOG0557, Eukaryota |
GeneTreei | ENSGT00940000154943 |
HOGENOMi | CLU_016733_10_2_1 |
InParanoidi | P08461 |
OMAi | TMEFESF |
OrthoDBi | 747232at2759 |
PhylomeDBi | P08461 |
TreeFami | TF106145 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR045257, E2/Pdx1 IPR036625, E3-bd_dom_sf IPR006257, LAT1 IPR004167, PSBD IPR011053, Single_hybrid_motif |
PANTHERi | PTHR23151, PTHR23151, 2 hits |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 2 hits PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 2 hits |
TIGRFAMsi | TIGR01349, PDHac_trf_mito, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 2 hits PS00189, LIPOYL, 2 hits PS51826, PSBD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MWRVCARRVQ SAVPRAGFRA RWATLKGPRT GPAAVRCGSG IPSYGVRSLC
60 70 80 90 100
GWSYGSATVP RNRILQQLLG SPSRRSYSLP PHQKVPLPSL SPTMQAGTIA
110 120 130 140 150
RWEKKEGEKI SEGDLIAEVE TDKATVGFES LEECYMAKIL VPEGTRDVPV
160 170 180 190 200
GSIICITVEK PQDIEAFKNY TLDSATAATQ AAPAPAAAPA AAPAAPSASA
210 220 230 240 250
PGSSYPVHMQ IVLPALSPTM TMGTVQRWEK KVGEKLSEGD LLAEIETDKA
260 270 280 290 300
TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKQEDI AAFADYRPTE
310 320 330 340 350
VTSLKPQAPP PVPPPVAAVP PIPQPLAPTP SAAPAGPKGR VFVSPLAKKL
360 370 380 390 400
AAEKGIDLTQ VKGTGPEGRI IKKDIDSFVP TKAAPAAAAA APPGPRVAPT
410 420 430 440 450
PAGVFIDIPI SNIRRVIAQR LMQSKQTIPH YYLSVDVNMG EVLLVRKELN
460 470 480 490 500
KMLEGKGKIS VNDFIIKASA LACLKVPEAN SSWMDTVIRQ NHVVDVSVAV
510 520 530 540 550
STPAGLITPI VFNAHIKGLE TIASDVVSLA SKAREGKLQP HEFQGGTFTI
560 570 580 590 600
SNLGMFGIKN FSAIINPPQA CILAIGASED KLIPADNEKG FDVASVMSVT
610 620 630
LSCDHRVVDG AVGAQWLAEF KKYLEKPVTM LL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 150 – 152 | VGS → IGC in BAA01504 (PubMed:1581353).Curated | 3 | |
Sequence conflicti | 163 – 164 | DI → GP in BAA20956 (PubMed:3571977).Curated | 2 | |
Sequence conflicti | 163 – 164 | DI → GP in AAA41813 (PubMed:3571977).Curated | 2 | |
Sequence conflicti | 601 – 632 | LSCDH…VTMLL → HSAVIIELWMEQLEPSGLL in BAA20956 (PubMed:3571977).CuratedAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC107440 mRNA Translation: AAI07441.1 D10655 mRNA Translation: BAA01504.1 D00092 mRNA Translation: BAA20956.1 M16075 mRNA Translation: AAA41813.1 |
PIRi | S21766 |
RefSeqi | NP_112287.1, NM_031025.1 |
Genome annotation databases
Ensembli | ENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994 |
GeneIDi | 81654 |
KEGGi | rno:81654 |
UCSCi | RGD:619859, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC107440 mRNA Translation: AAI07441.1 D10655 mRNA Translation: BAA01504.1 D00092 mRNA Translation: BAA20956.1 M16075 mRNA Translation: AAA41813.1 |
PIRi | S21766 |
RefSeqi | NP_112287.1, NM_031025.1 |
3D structure databases
AlphaFoldDBi | P08461 |
SMRi | P08461 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249554, 4 interactors |
IntActi | P08461, 4 interactors |
MINTi | P08461 |
STRINGi | 10116.ENSRNOP00000032890 |
PTM databases
iPTMneti | P08461 |
PhosphoSitePlusi | P08461 |
2D gel databases
World-2DPAGEi | 0004:P08461 |
Proteomic databases
jPOSTi | P08461 |
PaxDbi | P08461 |
PRIDEi | P08461 |
Genome annotation databases
Ensembli | ENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994 |
GeneIDi | 81654 |
KEGGi | rno:81654 |
UCSCi | RGD:619859, rat |
Organism-specific databases
CTDi | 1737 |
RGDi | 619859, Dlat |
Phylogenomic databases
eggNOGi | KOG0557, Eukaryota |
GeneTreei | ENSGT00940000154943 |
HOGENOMi | CLU_016733_10_2_1 |
InParanoidi | P08461 |
OMAi | TMEFESF |
OrthoDBi | 747232at2759 |
PhylomeDBi | P08461 |
TreeFami | TF106145 |
Enzyme and pathway databases
Reactomei | R-RNO-204174, Regulation of pyruvate dehydrogenase (PDH) complex R-RNO-389661, Glyoxylate metabolism and glycine degradation R-RNO-5362517, Signaling by Retinoic Acid R-RNO-70268, Pyruvate metabolism |
Miscellaneous databases
PROi | PR:P08461 |
Gene expression databases
Bgeei | ENSRNOG00000009994, Expressed in heart and 20 other tissues |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR045257, E2/Pdx1 IPR036625, E3-bd_dom_sf IPR006257, LAT1 IPR004167, PSBD IPR011053, Single_hybrid_motif |
PANTHERi | PTHR23151, PTHR23151, 2 hits |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 2 hits PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 2 hits |
TIGRFAMsi | TIGR01349, PDHac_trf_mito, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 2 hits PS00189, LIPOYL, 2 hits PS51826, PSBD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ODP2_RAT | |
Accessioni | P08461Primary (citable) accession number: P08461 Secondary accession number(s): Q3B7V7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | December 12, 2006 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families