UniProtKB - P08395 (SPPA_ECOLI)
Protein
Protease 4
Gene
sppA
Organism
Escherichia coli (strain K12)
Status
Functioni
Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane.4 Publications
Activity regulationi
Inhibited by serine hydrolase inhibitor FP-biotin and by antipain.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 209 | Proton donor/acceptorCurated | 1 | |
Active sitei | 409 | Nucleophile | 1 |
GO - Molecular functioni
- endopeptidase activity Source: EcoCyc
- serine-type peptidase activity Source: UniProtKB-KW
GO - Biological processi
- signal peptide processing Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10968-MONOMER MetaCyc:EG10968-MONOMER |
Protein family/group databases
MEROPSi | S49.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Protease 4 (EC:3.4.21.-)Alternative name(s): Endopeptidase IV Protease IV Signal peptide peptidase |
Gene namesi | Name:sppA Ordered Locus Names:b1766, JW1755 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalAdd BLAST | 21 | |
Topological domaini | 46 – 618 | PeriplasmicAdd BLAST | 573 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- membrane Source: UniProtKB
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
No effect on processing of liberated signal peptides in vivo.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 209 | K → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 366 | K → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 409 | S → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 410 | G → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 412 | Y → F: No effect on activity. 1 Publication | 1 | |
Mutagenesisi | 510 | H → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 524 | D → N: Increased activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171438 | 1 – 618 | Protease 4Add BLAST | 618 |
Proteomic databases
jPOSTi | P08395 |
PaxDbi | P08395 |
PRIDEi | P08395 |
Interactioni
Subunit structurei
Homotetramer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259140, 22 interactors |
IntActi | P08395, 1 interactor |
STRINGi | 511145.b1766 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08395 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08395 |
Family & Domainsi
Domaini
A tandem duplication in the protein fold creates an intact active site between the repeated domains of each monomer.
Sequence similaritiesi
Belongs to the peptidase S49 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_008856_1_1_6 |
InParanoidi | P08395 |
PhylomeDBi | P08395 |
Family and domain databases
CDDi | cd07019, S49_SppA_1, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004634, Pept_S49_pIV IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 IPR033854, S49_SppA_1 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 2 hits |
PIRSFi | PIRSF001217, Protease_4_SppA, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
TIGRFAMsi | TIGR00705, SppA_67K, 1 hit TIGR00706, SppA_dom, 1 hit |
i Sequence
Sequence statusi: Complete.
P08395-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWRFIAG FFKWTWRLLN FVREMVLNLF FIFLVLVGVG IWMQVSGGDS
60 70 80 90 100
KETASRGALL LDISGVIVDK PDSSQRFSKL SRQLLGASSD RLQENSLFDI
110 120 130 140 150
VNTIRQAKDD RNITGIVMDL KNFAGGDQPS MQYIGKALKE FRDSGKPVYA
160 170 180 190 200
VGENYSQGQY YLASFANKIW LSPQGVVDLH GFATNGLYYK SLLDKLKVST
210 220 230 240 250
HVFRVGTYKS AVEPFIRDDM SPAAREADSR WIGELWQNYL NTVAANRQIP
260 270 280 290 300
AEQVFPGAQG LLEGLTKTGG DTAKYALENK LVDALASSAE IEKALTKEFG
310 320 330 340 350
WSKTDKNYRA ISYYDYALKT PADTGDSIGV VFANGAIMDG EETQGNVGGD
360 370 380 390 400
TTAAQIRDAR LDPKVKAIVL RVNSPGGSVT ASEVIRAELA AARAAGKPVV
410 420 430 440 450
VSMGGMAASG GYWISTPANY IVANPSTLTG SIGIFGVITT VENSLDSIGV
460 470 480 490 500
HTDGVSTSPL ADVSITRALP PEAQLMMQLS IENGYKRFIT LVADARHSTP
510 520 530 540 550
EQIDKIAQGH VWTGQDAKAN GLVDSLGDFD DAVAKAAELA KVKQWHLEYY
560 570 580 590 600
VDEPTFFDKV MDNMSGSVRA MLPDAFQAML PAPLASVAST VKSESDKLAA
610
FNDPQNRYAF CLTCANMR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 378 | S → T in AAA24648 (PubMed:3522590).Curated | 1 | |
Sequence conflicti | 378 | S → T in AAA57009 (PubMed:7973728).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 151 | V → I in strain: ECOR 49 and ECOR 50. | 1 | |
Natural varianti | 186 | G → S in strain: ECOR 16. | 1 | |
Natural varianti | 252 | E → H in strain: ECOR 16. | 1 | |
Natural varianti | 252 | E → K in strain: ECOR 49. | 1 | |
Natural varianti | 252 | E → Q in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 50, ECOR 65 and ECOR 68. | 1 | |
Natural varianti | 294 | A → T in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 49, ECOR 50 and ECOR 65. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13359 Genomic DNA Translation: AAA24648.1 U00096 Genomic DNA Translation: AAC74836.1 AP009048 Genomic DNA Translation: BAA15557.1 U13772 Genomic DNA Translation: AAA57008.1 U13773 Genomic DNA Translation: AAA57009.1 U13774 Genomic DNA Translation: AAA57010.1 U13775 Genomic DNA Translation: AAA57011.1 U13776 Genomic DNA Translation: AAA57012.1 U13777 Genomic DNA Translation: AAA57013.1 U13778 Genomic DNA Translation: AAA57014.1 U13779 Genomic DNA Translation: AAA57015.1 U13780 Genomic DNA Translation: AAA57016.1 U13782 Genomic DNA Translation: AAA57017.1 U13833 Genomic DNA Translation: AAA57030.1 U13834 Genomic DNA Translation: AAA57031.1 |
PIRi | F64936, PRECT4 I81191 |
RefSeqi | NP_416280.1, NC_000913.3 WP_001259810.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74836; AAC74836; b1766 BAA15557; BAA15557; BAA15557 |
GeneIDi | 946281 |
KEGGi | ecj:JW1755 eco:b1766 |
PATRICi | fig|1411691.4.peg.488 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13359 Genomic DNA Translation: AAA24648.1 U00096 Genomic DNA Translation: AAC74836.1 AP009048 Genomic DNA Translation: BAA15557.1 U13772 Genomic DNA Translation: AAA57008.1 U13773 Genomic DNA Translation: AAA57009.1 U13774 Genomic DNA Translation: AAA57010.1 U13775 Genomic DNA Translation: AAA57011.1 U13776 Genomic DNA Translation: AAA57012.1 U13777 Genomic DNA Translation: AAA57013.1 U13778 Genomic DNA Translation: AAA57014.1 U13779 Genomic DNA Translation: AAA57015.1 U13780 Genomic DNA Translation: AAA57016.1 U13782 Genomic DNA Translation: AAA57017.1 U13833 Genomic DNA Translation: AAA57030.1 U13834 Genomic DNA Translation: AAA57031.1 |
PIRi | F64936, PRECT4 I81191 |
RefSeqi | NP_416280.1, NC_000913.3 WP_001259810.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BEZ | X-ray | 2.76 | A/B/C/D | 47-618 | [»] | |
3BF0 | X-ray | 2.55 | A/B/C/D | 47-618 | [»] | |
SMRi | P08395 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259140, 22 interactors |
IntActi | P08395, 1 interactor |
STRINGi | 511145.b1766 |
Protein family/group databases
MEROPSi | S49.001 |
Proteomic databases
jPOSTi | P08395 |
PaxDbi | P08395 |
PRIDEi | P08395 |
Genome annotation databases
EnsemblBacteriai | AAC74836; AAC74836; b1766 BAA15557; BAA15557; BAA15557 |
GeneIDi | 946281 |
KEGGi | ecj:JW1755 eco:b1766 |
PATRICi | fig|1411691.4.peg.488 |
Organism-specific databases
EchoBASEi | EB0961 |
Phylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_008856_1_1_6 |
InParanoidi | P08395 |
PhylomeDBi | P08395 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10968-MONOMER MetaCyc:EG10968-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P08395 |
PROi | PR:P08395 |
Family and domain databases
CDDi | cd07019, S49_SppA_1, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004634, Pept_S49_pIV IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 IPR033854, S49_SppA_1 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 2 hits |
PIRSFi | PIRSF001217, Protease_4_SppA, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
TIGRFAMsi | TIGR00705, SppA_67K, 1 hit TIGR00706, SppA_dom, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPPA_ECOLI | |
Accessioni | P08395Primary (citable) accession number: P08395 Secondary accession number(s): P77752 Q57183 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 29, 2003 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families