UniProtKB - P08392 (ICP4_HHV11)
Protein
Major viral transcription factor ICP4
Gene
ICP4
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Functioni
Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.7 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 262 – 490 | Add BLAST | 229 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA-templated viral transcription Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: InterPro
- response to type I interferon Source: BHF-UCL
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Major viral transcription factor ICP4Alternative name(s): Alpha-4 protein Infected cell protein 4 Transcriptional activator IE175 |
Gene namesi | Name:ICP4 Synonyms:IE175 ORF Names:RS1 |
Organismi | Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10299 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
Other locations
- Host nucleus 1 Publication
- Host cytoplasm 1 Publication
- Virion tegument 1 Publication
Note: Localizes to the cytoplasm when phosphorylated.By similarity
Other locations
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
- viral tegument Source: CACAO
Keywords - Cellular componenti
Host cytoplasm, Host nucleus, Virion, Virion tegumentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115815 | 1 – 1298 | Major viral transcription factor ICP4Add BLAST | 1298 |
Post-translational modificationi
ADP-ribosylated.
The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes.1 Publication
Keywords - PTMi
ADP-ribosylation, PhosphoproteinProteomic databases
PRIDEi | P08392 |
Interactioni
Subunit structurei
Forms homodimers (PubMed:2991559, PubMed:28505309).
Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions (PubMed:8995681).
Interacts with host TBP and host TAF1; these interactions help the stabilization of the pre-nitiation complex on specific promoters (PubMed:8649420).
Interacts with host GTF2B (PubMed:8392607).
5 PublicationsBinary interactionsi
P08392
With | #Exp. | IntAct |
---|---|---|
UL54 [P10238] | 5 | EBI-7185388,EBI-6883946 |
Protein-protein interaction databases
BioGRIDi | 971428, 1 interactor 971429, 10 interactors |
IntActi | P08392, 4 interactors |
MINTi | P08392 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P08392 |
SASBDBi | P08392 |
SMRi | P08392 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 116 – 121 | Poly-Asp | 6 | |
Compositional biasi | 166 – 169 | Poly-Arg | 4 | |
Compositional biasi | 176 – 199 | Poly-SerAdd BLAST | 24 | |
Compositional biasi | 236 – 243 | Poly-Pro | 8 | |
Compositional biasi | 321 – 324 | Poly-Pro | 4 | |
Compositional biasi | 446 – 453 | Poly-Ala | 8 | |
Compositional biasi | 502 – 508 | Poly-Ala | 7 | |
Compositional biasi | 613 – 616 | Poly-Pro | 4 | |
Compositional biasi | 683 – 688 | Poly-Ala | 6 | |
Compositional biasi | 702 – 707 | Poly-Ala | 6 | |
Compositional biasi | 710 – 722 | Poly-AlaAdd BLAST | 13 | |
Compositional biasi | 739 – 742 | Poly-Gly | 4 | |
Compositional biasi | 788 – 793 | Poly-Ala | 6 | |
Compositional biasi | 825 – 831 | Poly-Ala | 7 | |
Compositional biasi | 887 – 890 | Poly-Asp | 4 |
Domaini
The N-terminal and C-terminal domains are required for the transcriptional activation function of ICP4.1 Publication
Sequence similaritiesi
Belongs to the herpesviridae ICP4 family.Curated
Family and domain databases
DisProti | DP01305 |
InterProi | View protein in InterPro IPR005205, Herpes_ICP4_C IPR005206, Herpes_ICP4_N |
Pfami | View protein in Pfam PF03585, Herpes_ICP4_C, 1 hit PF03584, Herpes_ICP4_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P08392-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD
60 70 80 90 100
HPHDLDDARR DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT
110 120 130 140 150
PDPAASPPRT PAFRADDDDG DEYDDAADAA GDRAPARGRE REAPLRGAYP
160 170 180 190 200
DPTDRLSPRP PAQPPRRRRH GRWRPSASST SSDSGSSSSS SASSSSSSSD
210 220 230 240 250
EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG PPPLSEAAPK
260 270 280 290 300
PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG
310 320 330 340 350
AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR
360 370 380 390 400
RFEASGAPAA VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD
410 420 430 440 450
VALDQACFRI SGAARNSSSF ITGSVARAVP HLGYAMAAGR FGWGLAHAAA
460 470 480 490 500
AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR ENAALTGAAG SPGAGADDEG
510 520 530 540 550
VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG DDPDAARHAD
560 570 580 590 600
ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS
610 620 630 640 650
PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG
660 670 680 690 700
GSEAAVAAVR AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL
710 720 730 740 750
LAAAASAPDA ADALAAAAAS AAPREGRKRK SPGPARPPGG GGPRPPKTKK
760 770 780 790 800
SGADAPGSDA RAPLPAPAPP STPPGPEPAP AQPAAPRAAA AQARPRPVAV
810 820 830 840 850
SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA VAELTDHPLF
860 870 880 890 900
PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG
910 920 930 940 950
RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG
960 970 980 990 1000
PPEWSAERGG LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL
1010 1020 1030 1040 1050
LSTRDLAFAG AVEFLGLLAS AGDRRLIVVN TVRACDWPAD GPAVSRQHAY
1060 1070 1080 1090 1100
LACELLPAVQ CAVRWPAARD LRRTVLASGR VFGPGVFARV EAAHARLYPD
1110 1120 1130 1140 1150
APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA LDGRAAASGT
1160 1170 1180 1190 1200
TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW
1210 1220 1230 1240 1250
RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS
1260 1270 1280 1290
GTVLAAAGAV EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32286.1 X14112 Genomic DNA Translation: CAA32278.1 X06461 Genomic DNA Translation: CAA29763.1 L00036 Genomic DNA Translation: AAA96675.1 L00036 Genomic DNA Translation: AAA96688.1 |
PIRi | A23510, EDBE75 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32286.1 X14112 Genomic DNA Translation: CAA32278.1 X06461 Genomic DNA Translation: CAA29763.1 L00036 Genomic DNA Translation: AAA96675.1 L00036 Genomic DNA Translation: AAA96688.1 |
PIRi | A23510, EDBE75 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MHJ | X-ray | 2.12 | A/B | 288-487 | [»] | |
5MHK | X-ray | 2.28 | A/B/C/D | 258-487 | [»] | |
J | 283-286 | [»] | ||||
BMRBi | P08392 | |||||
SASBDBi | P08392 | |||||
SMRi | P08392 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 971428, 1 interactor 971429, 10 interactors |
IntActi | P08392, 4 interactors |
MINTi | P08392 |
Proteomic databases
PRIDEi | P08392 |
Family and domain databases
DisProti | DP01305 |
InterProi | View protein in InterPro IPR005205, Herpes_ICP4_C IPR005206, Herpes_ICP4_N |
Pfami | View protein in Pfam PF03585, Herpes_ICP4_C, 1 hit PF03584, Herpes_ICP4_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ICP4_HHV11 | |
Accessioni | P08392Primary (citable) accession number: P08392 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families