Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell wall formation.

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.

Cofactori

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1591
Binding sitei190Substrate1
Active sitei229Proton donor1
Active sitei3251

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: EcoliWiki
  • peptidoglycan biosynthetic process Source: EcoliWiki
  • regulation of cell shape Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciEcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
SABIO-RKiP08373
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase (EC:1.3.1.98)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenase
Gene namesi
Name:murB
Synonyms:yijB
Ordered Locus Names:b3972, JW3940
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11205 murB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5526
DrugBankiDB07296 (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE
DB03147 Flavin adenine dinucleotide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001792071 – 342UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST342

Proteomic databases

PaxDbiP08373
PRIDEiP08373

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262982, 592 interactors
DIPiDIP-10277N
IntActiP08373, 12 interactors
STRINGi316385.ECDH10B_4161

Chemistry databases

BindingDBiP08373

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08373
SMRiP08373
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08373

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 183FAD-binding PCMH-typeAdd BLAST171

Sequence similaritiesi

Belongs to the MurB family.Curated

Phylogenomic databases

eggNOGiENOG4105D4A Bacteria
COG0812 LUCA
HOGENOMiHOG000284356
InParanoidiP08373
KOiK00075
OMAiIGNAGSF
PhylomeDBiP08373

Family and domain databases

Gene3Di3.30.43.10, 1 hit
3.90.78.10, 1 hit
HAMAPiMF_00037 MurB, 1 hit
InterProiView protein in InterPro
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR003170 MurB
IPR011601 MurB_C
IPR036635 MurB_C_sf
IPR006094 Oxid_FAD_bind_N
PANTHERiPTHR21071 PTHR21071, 1 hit
PfamiView protein in Pfam
PF01565 FAD_binding_4, 1 hit
PF02873 MurB_C, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
SSF56194 SSF56194, 1 hit
TIGRFAMsiTIGR00179 murB, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

P08373-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNHSLKPWNT FGIDHNAQHI VCAEDEQQLL NAWQYATAEG QPVLILGEGS
60 70 80 90 100
NVLFLEDYRG TVIINRIKGI EIHDEPDAWY LHVGAGENWH RLVKYTLQEG
110 120 130 140 150
MPGLENLALI PGCVGSSPIQ NIGAYGVELQ RVCAYVDSVE LATGKQVRLT
160 170 180 190 200
AKECRFGYRD SIFKHEYQDR FAIVAVGLRL PKEWQPVLTY GDLTRLDPTT
210 220 230 240 250
VTPQQVFNAV CHMRTTKLPD PKVNGNAGSF FKNPVVSAET AKALLSQFPT
260 270 280 290 300
APNYPQADGS VKLAAGWLID QCQLKGMQIG GAAVHRQQAL VLINEDNAKS
310 320 330 340
EDVVQLAHHV RQKVGEKFNV WLEPEVRFIG ASGEVSAVET IS
Length:342
Mass (Da):37,851
Last modified:August 1, 1988 - v1
Checksum:iF43CFE29251E45C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35Y → H in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti138S → C in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti242K → E in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti279I → M in AAA24185 (PubMed:1311302).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10123 Genomic DNA Translation: AAA23519.1
L14557 Genomic DNA Translation: AAA24185.1
U00006 Genomic DNA Translation: AAC43074.1
U00096 Genomic DNA Translation: AAC76950.1
AP009048 Genomic DNA Translation: BAE77343.1
V00348 Genomic DNA No translation available.
PIRiA24029 QQECB8
RefSeqiNP_418403.1, NC_000913.3
WP_001016699.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76950; AAC76950; b3972
BAE77343; BAE77343; BAE77343
GeneIDi948470
KEGGiecj:JW3940
eco:b3972
PATRICifig|1411691.4.peg.2736

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10123 Genomic DNA Translation: AAA23519.1
L14557 Genomic DNA Translation: AAA24185.1
U00006 Genomic DNA Translation: AAC43074.1
U00096 Genomic DNA Translation: AAC76950.1
AP009048 Genomic DNA Translation: BAE77343.1
V00348 Genomic DNA No translation available.
PIRiA24029 QQECB8
RefSeqiNP_418403.1, NC_000913.3
WP_001016699.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MBBX-ray2.30A1-342[»]
1MBTX-ray3.00A1-342[»]
1UXYX-ray1.80A3-342[»]
2MBRX-ray1.80A3-342[»]
2Q85X-ray2.51A1-342[»]
ProteinModelPortaliP08373
SMRiP08373
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262982, 592 interactors
DIPiDIP-10277N
IntActiP08373, 12 interactors
STRINGi316385.ECDH10B_4161

Chemistry databases

BindingDBiP08373
ChEMBLiCHEMBL5526
DrugBankiDB07296 (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE
DB03147 Flavin adenine dinucleotide

Proteomic databases

PaxDbiP08373
PRIDEiP08373

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76950; AAC76950; b3972
BAE77343; BAE77343; BAE77343
GeneIDi948470
KEGGiecj:JW3940
eco:b3972
PATRICifig|1411691.4.peg.2736

Organism-specific databases

EchoBASEiEB1190
EcoGeneiEG11205 murB

Phylogenomic databases

eggNOGiENOG4105D4A Bacteria
COG0812 LUCA
HOGENOMiHOG000284356
InParanoidiP08373
KOiK00075
OMAiIGNAGSF
PhylomeDBiP08373

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciEcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
SABIO-RKiP08373

Miscellaneous databases

EvolutionaryTraceiP08373
PROiPR:P08373

Family and domain databases

Gene3Di3.30.43.10, 1 hit
3.90.78.10, 1 hit
HAMAPiMF_00037 MurB, 1 hit
InterProiView protein in InterPro
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR003170 MurB
IPR011601 MurB_C
IPR036635 MurB_C_sf
IPR006094 Oxid_FAD_bind_N
PANTHERiPTHR21071 PTHR21071, 1 hit
PfamiView protein in Pfam
PF01565 FAD_binding_4, 1 hit
PF02873 MurB_C, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
SSF56194 SSF56194, 1 hit
TIGRFAMsiTIGR00179 murB, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMURB_ECOLI
AccessioniPrimary (citable) accession number: P08373
Secondary accession number(s): Q2M8R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 10, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again