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UniProtKB - P08373 (MURB_ECOLI)
Protein
UDP-N-acetylenolpyruvoylglucosamine reductase
Gene
murB
Organism
Escherichia coli (strain K12)
Status
Functioni
Cell wall formation.
Catalytic activityi
- EC:1.3.1.98
Cofactori
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 159 | 1 | ||
Binding sitei | 190 | Substrate | 1 | |
Active sitei | 229 | Proton donor | 1 | |
Active sitei | 325 | 1 |
GO - Molecular functioni
- FAD binding Source: InterPro
- flavin adenine dinucleotide binding Source: EcoliWiki
- UDP-N-acetylmuramate dehydrogenase activity Source: EcoCyc
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: EcoliWiki
- peptidoglycan biosynthetic process Source: EcoliWiki
- regulation of cell shape Source: EcoliWiki
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER |
SABIO-RKi | P08373 |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylenolpyruvoylglucosamine reductase (EC:1.3.1.98)Alternative name(s): UDP-N-acetylmuramate dehydrogenase |
Gene namesi | Name:murB Synonyms:yijB Ordered Locus Names:b3972, JW3940 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL5526 |
DrugBanki | DB07296, (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE DB03147, Flavin adenine dinucleotide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000179207 | 1 – 342 | UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST | 342 |
Proteomic databases
jPOSTi | P08373 |
PaxDbi | P08373 |
PRIDEi | P08373 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262982, 592 interactors 852766, 2 interactors |
DIPi | DIP-10277N |
IntActi | P08373, 12 interactors |
STRINGi | 511145.b3972 |
Chemistry databases
BindingDBi | P08373 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08373 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08373 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 183 | FAD-binding PCMH-typeAdd BLAST | 171 |
Sequence similaritiesi
Belongs to the MurB family.Curated
Phylogenomic databases
eggNOGi | COG0812, Bacteria |
HOGENOMi | CLU_035304_0_0_6 |
InParanoidi | P08373 |
PhylomeDBi | P08373 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.90.78.10, 1 hit |
HAMAPi | MF_00037, MurB, 1 hit |
InterProi | View protein in InterPro IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR003170, MurB IPR011601, MurB_C IPR036635, MurB_C_sf IPR006094, Oxid_FAD_bind_N |
PANTHERi | PTHR21071, PTHR21071, 1 hit |
Pfami | View protein in Pfam PF01565, FAD_binding_4, 1 hit PF02873, MurB_C, 1 hit |
SUPFAMi | SSF56176, SSF56176, 1 hit SSF56194, SSF56194, 1 hit |
TIGRFAMsi | TIGR00179, murB, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
i Sequence
Sequence statusi: Complete.
P08373-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNHSLKPWNT FGIDHNAQHI VCAEDEQQLL NAWQYATAEG QPVLILGEGS
60 70 80 90 100
NVLFLEDYRG TVIINRIKGI EIHDEPDAWY LHVGAGENWH RLVKYTLQEG
110 120 130 140 150
MPGLENLALI PGCVGSSPIQ NIGAYGVELQ RVCAYVDSVE LATGKQVRLT
160 170 180 190 200
AKECRFGYRD SIFKHEYQDR FAIVAVGLRL PKEWQPVLTY GDLTRLDPTT
210 220 230 240 250
VTPQQVFNAV CHMRTTKLPD PKVNGNAGSF FKNPVVSAET AKALLSQFPT
260 270 280 290 300
APNYPQADGS VKLAAGWLID QCQLKGMQIG GAAVHRQQAL VLINEDNAKS
310 320 330 340
EDVVQLAHHV RQKVGEKFNV WLEPEVRFIG ASGEVSAVET IS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | Y → H in AAA24185 (PubMed:1311302).Curated | 1 | |
Sequence conflicti | 138 | S → C in AAA24185 (PubMed:1311302).Curated | 1 | |
Sequence conflicti | 242 | K → E in AAA24185 (PubMed:1311302).Curated | 1 | |
Sequence conflicti | 279 | I → M in AAA24185 (PubMed:1311302).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10123 Genomic DNA Translation: AAA23519.1 L14557 Genomic DNA Translation: AAA24185.1 U00006 Genomic DNA Translation: AAC43074.1 U00096 Genomic DNA Translation: AAC76950.1 AP009048 Genomic DNA Translation: BAE77343.1 V00348 Genomic DNA No translation available. |
PIRi | A24029, QQECB8 |
RefSeqi | NP_418403.1, NC_000913.3 WP_001016699.1, NZ_SSZK01000084.1 |
Genome annotation databases
EnsemblBacteriai | AAC76950; AAC76950; b3972 BAE77343; BAE77343; BAE77343 |
GeneIDi | 948470 |
KEGGi | ecj:JW3940 eco:b3972 |
PATRICi | fig|1411691.4.peg.2736 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10123 Genomic DNA Translation: AAA23519.1 L14557 Genomic DNA Translation: AAA24185.1 U00006 Genomic DNA Translation: AAC43074.1 U00096 Genomic DNA Translation: AAC76950.1 AP009048 Genomic DNA Translation: BAE77343.1 V00348 Genomic DNA No translation available. |
PIRi | A24029, QQECB8 |
RefSeqi | NP_418403.1, NC_000913.3 WP_001016699.1, NZ_SSZK01000084.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MBB | X-ray | 2.30 | A | 1-342 | [»] | |
1MBT | X-ray | 3.00 | A | 1-342 | [»] | |
1UXY | X-ray | 1.80 | A | 3-342 | [»] | |
2MBR | X-ray | 1.80 | A | 3-342 | [»] | |
2Q85 | X-ray | 2.51 | A | 1-342 | [»] | |
SMRi | P08373 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262982, 592 interactors 852766, 2 interactors |
DIPi | DIP-10277N |
IntActi | P08373, 12 interactors |
STRINGi | 511145.b3972 |
Chemistry databases
BindingDBi | P08373 |
ChEMBLi | CHEMBL5526 |
DrugBanki | DB07296, (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE DB03147, Flavin adenine dinucleotide |
Proteomic databases
jPOSTi | P08373 |
PaxDbi | P08373 |
PRIDEi | P08373 |
Genome annotation databases
EnsemblBacteriai | AAC76950; AAC76950; b3972 BAE77343; BAE77343; BAE77343 |
GeneIDi | 948470 |
KEGGi | ecj:JW3940 eco:b3972 |
PATRICi | fig|1411691.4.peg.2736 |
Organism-specific databases
EchoBASEi | EB1190 |
Phylogenomic databases
eggNOGi | COG0812, Bacteria |
HOGENOMi | CLU_035304_0_0_6 |
InParanoidi | P08373 |
PhylomeDBi | P08373 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER |
SABIO-RKi | P08373 |
Miscellaneous databases
EvolutionaryTracei | P08373 |
PROi | PR:P08373 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.90.78.10, 1 hit |
HAMAPi | MF_00037, MurB, 1 hit |
InterProi | View protein in InterPro IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR003170, MurB IPR011601, MurB_C IPR036635, MurB_C_sf IPR006094, Oxid_FAD_bind_N |
PANTHERi | PTHR21071, PTHR21071, 1 hit |
Pfami | View protein in Pfam PF01565, FAD_binding_4, 1 hit PF02873, MurB_C, 1 hit |
SUPFAMi | SSF56176, SSF56176, 1 hit SSF56194, SSF56194, 1 hit |
TIGRFAMsi | TIGR00179, murB, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MURB_ECOLI | |
Accessioni | P08373Primary (citable) accession number: P08373 Secondary accession number(s): Q2M8R3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families