UniProtKB - P08373 (MURB_ECOLI)

BLAST

>sp|P08373|MURB_ECOLI UDP-N-acetylenolpyruvoylglucosamine reductase OS=Escherichia coli (strain K12) OX=83333 GN=murB PE=1 SV=1
MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVLILGEGSNVLFLEDYRG
TVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMPGLENLALIPGCVGSSPIQ
NIGAYGVELQRVCAYVDSVELATGKQVRLTAKECRFGYRDSIFKHEYQDRFAIVAVGLRL
PKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKLPDPKVNGNAGSFFKNPVVSAET
AKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKGMQIGGAAVHRQQALVLINEDNAKS
EDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGEVSAVETIS

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History
Entry version 176 (10 Oct 2018)
Sequence version 1 (01 Aug 1988)
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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Cell wall formation.

Catalytic activityⁱ

UDP-N-acetyl-alpha-D-muramate + NADP⁺ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.

Cofactorⁱ

FAD

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	159		1
Binding siteⁱ	190	Substrate	1
Active siteⁱ	229	Proton donor	1
Active siteⁱ	325		1

GO - Molecular functionⁱ

FAD binding Source: InterPro
flavin adenine dinucleotide binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 8448160
UDP-N-acetylmuramate dehydrogenase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 7727397

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
cell wall organization
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 16436710
peptidoglycan biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 16436710
regulation of cell shape
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 16436710

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
Ligand	FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCycⁱ	EcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
SABIO-RKⁱ	P08373
UniPathwayⁱ	UPA00219

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: UDP-N-acetylenolpyruvoylglucosamine reductase (EC:1.3.1.98) Alternative name(s): UDP-N-acetylmuramate dehydrogenase
Gene namesⁱ	Name:murB Synonyms:yijB Ordered Locus Names:b3972, JW3940
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11205 murB

EcoGeneⁱ

EG11205 murB

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytoplasm
Source: EcoliWiki
Inferred from direct assayⁱ
- 16436710
cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL5526
Drug and drug target database More...DrugBankⁱ	DB07296 (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE DB03147 Flavin adenine dinucleotide

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000179207	1 – 342	UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST		342

Proteomic databases

PaxDbⁱ	P08373
PRIDEⁱ	P08373

Interactionⁱ

Subunit structureⁱ

Monomer.

Protein-protein interaction databases

BioGridⁱ	4262982, 592 interactors
Database of interacting proteins More...DIPⁱ	DIP-10277N
IntActⁱ	P08373, 12 interactors
STRINGⁱ	316385.ECDH10B_4161

Chemistry databases

BindingDBⁱ

P08373

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	6 – 8	Combined sources	3
Beta strandⁱ	16 – 25	Combined sources	10
Helixⁱ	26 – 38	Combined sources	13
Beta strandⁱ	43 – 48	Combined sources	6
Beta strandⁱ	52 – 54	Combined sources	3
Beta strandⁱ	58 – 65	Combined sources	8
Beta strandⁱ	70 – 74	Combined sources	5
Beta strandⁱ	76 – 84	Combined sources	9
Helixⁱ	89 – 98	Combined sources	10
Helixⁱ	105 – 107	Combined sources	3
Helixⁱ	114 – 116	Combined sources	3
Turnⁱ	117 – 121	Combined sources	5
Helixⁱ	129 – 131	Combined sources	3
Beta strandⁱ	133 – 140	Combined sources	8
Turnⁱ	141 – 143	Combined sources	3
Beta strandⁱ	146 – 150	Combined sources	5
Helixⁱ	152 – 154	Combined sources	3
Helixⁱ	162 – 164	Combined sources	3
Turnⁱ	165 – 170	Combined sources	6
Beta strandⁱ	171 – 183	Combined sources	13
Helixⁱ	191 – 195	Combined sources	5
Turnⁱ	198 – 200	Combined sources	3
Helixⁱ	203 – 217	Combined sources	15
Turnⁱ	221 – 223	Combined sources	3
Beta strandⁱ	226 – 231	Combined sources	6
Helixⁱ	238 – 247	Combined sources	10
Beta strandⁱ	253 – 255	Combined sources	3
Beta strandⁱ	261 – 263	Combined sources	3
Helixⁱ	265 – 271	Combined sources	7
Beta strandⁱ	282 – 284	Combined sources	3
Beta strandⁱ	286 – 288	Combined sources	3
Beta strandⁱ	291 – 294	Combined sources	4
Helixⁱ	300 – 318	Combined sources	19
Beta strandⁱ	326 – 330	Combined sources	5
Beta strandⁱ	333 – 335	Combined sources	3
Helixⁱ	337 – 341	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	P08373
SMRⁱ	P08373
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P08373

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	13 – 183	FAD-binding PCMH-typeAdd BLAST		171

Sequence similaritiesⁱ

Belongs to the MurB family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105D4A Bacteria COG0812 LUCA
HOGENOMⁱ	HOG000284356
InParanoidⁱ	P08373
KEGG Orthology (KO) More...KOⁱ	K00075
OMAⁱ	IGNAGSF
PhylomeDBⁱ	P08373

Family and domain databases

Gene3Dⁱ	3.30.43.10, 1 hit 3.90.78.10, 1 hit
HAMAPⁱ	MF_00037 MurB, 1 hit
InterProⁱ	View protein in InterPro IPR016166 FAD-bd_PCMH IPR036318 FAD-bd_PCMH-like_sf IPR016167 FAD-bd_PCMH_sub1 IPR003170 MurB IPR011601 MurB_C IPR036635 MurB_C_sf IPR006094 Oxid_FAD_bind_N
PANTHERⁱ	PTHR21071 PTHR21071, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01565 FAD_binding_4, 1 hit PF02873 MurB_C, 1 hit
SUPFAMⁱ	SSF56176 SSF56176, 1 hit SSF56194 SSF56194, 1 hit
TIGRFAMsⁱ	TIGR00179 murB, 1 hit
PROSITEⁱ	View protein in PROSITE PS51387 FAD_PCMH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P08373-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNHSLKPWNT FGIDHNAQHI VCAEDEQQLL NAWQYATAEG QPVLILGEGS 
        60         70         80         90        100
NVLFLEDYRG TVIINRIKGI EIHDEPDAWY LHVGAGENWH RLVKYTLQEG 
       110        120        130        140        150
MPGLENLALI PGCVGSSPIQ NIGAYGVELQ RVCAYVDSVE LATGKQVRLT 
       160        170        180        190        200
AKECRFGYRD SIFKHEYQDR FAIVAVGLRL PKEWQPVLTY GDLTRLDPTT 
       210        220        230        240        250
VTPQQVFNAV CHMRTTKLPD PKVNGNAGSF FKNPVVSAET AKALLSQFPT 
       260        270        280        290        300
APNYPQADGS VKLAAGWLID QCQLKGMQIG GAAVHRQQAL VLINEDNAKS 
       310        320        330        340 
EDVVQLAHHV RQKVGEKFNV WLEPEVRFIG ASGEVSAVET IS

Length:342

Mass (Da):37,851

Last modified:August 1, 1988 - v1

Checksum:ⁱF43CFE29251E45C6

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	35	Y → H in AAA24185 (PubMed:1311302).Curated	1
Sequence conflictⁱ	138	S → C in AAA24185 (PubMed:1311302).Curated	1
Sequence conflictⁱ	242	K → E in AAA24185 (PubMed:1311302).Curated	1
Sequence conflictⁱ	279	I → M in AAA24185 (PubMed:1311302).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M10123 Genomic DNA Translation: AAA23519.1 L14557 Genomic DNA Translation: AAA24185.1 U00006 Genomic DNA Translation: AAC43074.1 U00096 Genomic DNA Translation: AAC76950.1 AP009048 Genomic DNA Translation: BAE77343.1 V00348 Genomic DNA No translation available.
PIRⁱ	A24029 QQECB8
NCBI Reference Sequences More...RefSeqⁱ	NP_418403.1, NC_000913.3 WP_001016699.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76950; AAC76950; b3972 BAE77343; BAE77343; BAE77343
GeneIDⁱ	948470
KEGGⁱ	ecj:JW3940 eco:b3972
PATRICⁱ	fig\|1411691.4.peg.2736

Protein	Similar proteins		Species	Score	Length	Source
P08373	UDP-N-acetylenolpyruvoylglucosamine reductase		ECOLX		342	UniRef100_P08373
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIDY		342
UDP-N-acetylenolpyruvoylglucosamine reductase		Escherichia coli 1-110-08_S3_C1		342
UDP-N-acetylenolpyruvoylglucosamine reductase		Escherichia coli UMNK88		342
UDP-N-acetylenolpyruvoylglucosamine reductase		Escherichia coli DEC6A		342
+19

Protein	Similar proteins		Species	Score	Length	Source
P08373	UDP-N-acetylenolpyruvoylglucosamine reductase		ECO57		342	UniRef90_P08373
UDP-N-acetylenolpyruvoylglucosamine reductase		ECOL6		342
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIBS		342
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIDS		342
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIFL		342
+569

Protein	Similar proteins		Species	Score	Length	Source
P08373	UDP-N-acetylenolpyruvoylglucosamine reductase		SHIFL		342	UniRef50_P08373
UDP-N-acetylenolpyruvoylglucosamine reductase		SHISS		342
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIBS		342
UDP-N-acetylenolpyruvoylglucosamine reductase		ECOL6		342
UDP-N-acetylenolpyruvoylglucosamine reductase		SHIDS		342
+644

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M10123 Genomic DNA Translation: AAA23519.1 L14557 Genomic DNA Translation: AAA24185.1 U00006 Genomic DNA Translation: AAC43074.1 U00096 Genomic DNA Translation: AAC76950.1 AP009048 Genomic DNA Translation: BAE77343.1 V00348 Genomic DNA No translation available.
PIRⁱ	A24029 QQECB8
RefSeqⁱ	NP_418403.1, NC_000913.3 WP_001016699.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1MBB	X-ray	2.30	A	1-342	[»]
	1MBT	X-ray	3.00	A	1-342	[»]
	1UXY	X-ray	1.80	A	3-342	[»]
	2MBR	X-ray	1.80	A	3-342	[»]
	2Q85	X-ray	2.51	A	1-342	[»]
ProteinModelPortalⁱ	P08373
SMRⁱ	P08373
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262982, 592 interactors
DIPⁱ	DIP-10277N
IntActⁱ	P08373, 12 interactors
STRINGⁱ	316385.ECDH10B_4161

Chemistry databases

BindingDBⁱ	P08373
ChEMBLⁱ	CHEMBL5526
DrugBankⁱ	DB07296 (5Z)-3-(4-CHLOROPHENYL)-4-HYDROXY-5-(1-NAPHTHYLMETHYLENE)FURAN-2(5H)-ONE DB03147 Flavin adenine dinucleotide

Proteomic databases

PaxDbⁱ	P08373
PRIDEⁱ	P08373

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76950; AAC76950; b3972 BAE77343; BAE77343; BAE77343
GeneIDⁱ	948470
KEGGⁱ	ecj:JW3940 eco:b3972
PATRICⁱ	fig\|1411691.4.peg.2736

Organism-specific databases

EchoBASEⁱ	EB1190
EcoGeneⁱ	EG11205 murB

Phylogenomic databases

eggNOGⁱ	ENOG4105D4A Bacteria COG0812 LUCA
HOGENOMⁱ	HOG000284356
InParanoidⁱ	P08373
KOⁱ	K00075
OMAⁱ	IGNAGSF
PhylomeDBⁱ	P08373

Enzyme and pathway databases

UniPathwayⁱ	UPA00219
BioCycⁱ	EcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER
SABIO-RKⁱ	P08373

Miscellaneous databases

EvolutionaryTraceⁱ	P08373
Protein Ontology More...PROⁱ	PR:P08373

Family and domain databases

Gene3Dⁱ	3.30.43.10, 1 hit 3.90.78.10, 1 hit
HAMAPⁱ	MF_00037 MurB, 1 hit
InterProⁱ	View protein in InterPro IPR016166 FAD-bd_PCMH IPR036318 FAD-bd_PCMH-like_sf IPR016167 FAD-bd_PCMH_sub1 IPR003170 MurB IPR011601 MurB_C IPR036635 MurB_C_sf IPR006094 Oxid_FAD_bind_N
PANTHERⁱ	PTHR21071 PTHR21071, 1 hit
Pfamⁱ	View protein in Pfam PF01565 FAD_binding_4, 1 hit PF02873 MurB_C, 1 hit
SUPFAMⁱ	SSF56176 SSF56176, 1 hit SSF56194 SSF56194, 1 hit
TIGRFAMsⁱ	TIGR00179 murB, 1 hit
PROSITEⁱ	View protein in PROSITE PS51387 FAD_PCMH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MURB_ECOLI
Accessionⁱ	P08373Primary (citable) accession number: P08373 Secondary accession number(s): Q2M8R3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	August 1, 1988
	Last modified:	October 10, 2018
	This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08373 (MURB_ECOLI)

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UDP-N-acetylenolpyruvoylglucosamine reductase

murB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ