UniProtKB - P08337 (MUTT_ECOLI)
Protein
8-oxo-dGTP diphosphatase
Gene
mutT
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate.2 Publications
Catalytic activityi
- EC:3.6.1.551 Publication
Cofactori
Mg2+Note: Binds 1 Mg2+ ion per subunit.
Kineticsi
- KM=0.058 µM for 8-oxo-dGDP1 Publication
- KM=170 µM for dGDP1 Publication
- KM=0.081 µM for 8-oxo-dGTP1 Publication
- KM=1100 µM for GTP1 Publication
- KM=0.045 µM for 8-oxo-GDP1 Publication
- KM=0.26 µM for 8-oxo-GTP1 Publication
- Vmax=3.7 pmol/min/ng enzyme toward 8-oxo-dGDP1 Publication
- Vmax=5.9 pmol/min/ng enzyme toward dGDP1 Publication
- Vmax=20 pmol/min/ng enzyme toward 8-oxo-dGTP1 Publication
- Vmax=44 pmol/min/ng enzyme toward GTP1 Publication
- Vmax=4.8 pmol/min/ng enzyme toward 8-oxo-GDP1 Publication
- Vmax=22 pmol/min/ng enzyme toward 8-oxo-GTP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 23 | Substrate | 1 | |
Binding sitei | 28 | Substrate | 1 | |
Metal bindingi | 38 | Magnesium; via carbonyl oxygen | 1 | |
Metal bindingi | 53 | Magnesium | 1 | |
Metal bindingi | 56 | Magnesium | 1 | |
Metal bindingi | 57 | Magnesium | 1 | |
Binding sitei | 119 | Substrate | 1 |
GO - Molecular functioni
- 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity Source: EcoCyc
- 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity Source: UniProtKB
- 8-oxo-dGDP phosphatase activity Source: EcoCyc
- 8-oxo-GDP phosphatase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
- manganese ion binding Source: EcoCyc
GO - Biological processi
- DNA repair Source: GO_Central
- DNA replication Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Mutator protein |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10626-MONOMER MetaCyc:EG10626-MONOMER |
BRENDAi | 3.6.1.55, 2026 |
SABIO-RKi | P08337 |
Names & Taxonomyi
Protein namesi | Recommended name: 8-oxo-dGTP diphosphatase (EC:3.6.1.55)Short name: 8-oxo-dGTPase Alternative name(s): 7,8-dihydro-8-oxoguanine-triphosphatase Mutator protein MutT dGTP pyrophosphohydrolase |
Gene namesi | Name:mutT Ordered Locus Names:b0099, JW0097 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056943 | 1 – 129 | 8-oxo-dGTP diphosphataseAdd BLAST | 129 |
Proteomic databases
jPOSTi | P08337 |
PaxDbi | P08337 |
PRIDEi | P08337 |
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
P08337
With | #Exp. | IntAct |
---|---|---|
frdB [P0AC47] | 4 | EBI-1121389,EBI-906724 |
Protein-protein interaction databases
BioGRIDi | 4261888, 366 interactors 849225, 4 interactors |
DIPi | DIP-10288N |
IntActi | P08337, 5 interactors |
STRINGi | 511145.b0099 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P08337 |
SMRi | P08337 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08337 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 129 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 6 – 8 | Substrate binding | 3 | |
Regioni | 34 – 38 | Substrate binding | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 38 – 59 | Nudix boxAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the Nudix hydrolase family.Curated
Phylogenomic databases
eggNOGi | COG0494, Bacteria |
HOGENOMi | CLU_037162_19_2_6 |
InParanoidi | P08337 |
PhylomeDBi | P08337 |
Family and domain databases
InterProi | View protein in InterPro IPR003561, Mutator_MutT IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01401, MUTATORMUTT PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
TIGRFAMsi | TIGR00586, mutt, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
i Sequence
Sequence statusi: Complete.
P08337-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLQIAVGI IRNENNEIFI TRRAADAHMA NKLEFPGGKI EMGETPEQAV
60 70 80 90 100
VRELQEEVGI TPQHFSLFEK LEYEFPDRHI TLWFWLVERW EGEPWGKEGQ
110 120
PGEWMSLVGL NADDFPPANE PVIAKLKRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04831 Genomic DNA Translation: CAA28523.1 M20791 Genomic DNA Translation: AAA24620.1 X55034 Genomic DNA Translation: CAA38876.1 U00096 Genomic DNA Translation: AAC73210.1 AP009048 Genomic DNA Translation: BAB96667.1 |
PIRi | A27890, MVECMT |
RefSeqi | NP_414641.1, NC_000913.3 WP_000736007.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73210; AAC73210; b0099 BAB96667; BAB96667; BAB96667 |
GeneIDi | 944824 |
KEGGi | ecj:JW0097 eco:b0099 |
PATRICi | fig|1411691.4.peg.2181 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04831 Genomic DNA Translation: CAA28523.1 M20791 Genomic DNA Translation: AAA24620.1 X55034 Genomic DNA Translation: CAA38876.1 U00096 Genomic DNA Translation: AAC73210.1 AP009048 Genomic DNA Translation: BAB96667.1 |
PIRi | A27890, MVECMT |
RefSeqi | NP_414641.1, NC_000913.3 WP_000736007.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MUT | NMR | - | A | 1-129 | [»] | |
1PPX | NMR | - | A | 1-129 | [»] | |
1PUN | NMR | - | A | 1-129 | [»] | |
1PUQ | NMR | - | A | 1-129 | [»] | |
1PUS | NMR | - | A | 1-129 | [»] | |
1TUM | NMR | - | A | 1-129 | [»] | |
3A6S | X-ray | 1.80 | A/B | 1-129 | [»] | |
3A6T | X-ray | 1.96 | A | 1-129 | [»] | |
3A6U | X-ray | 2.56 | A | 1-129 | [»] | |
3A6V | X-ray | 2.00 | A/B | 1-129 | [»] | |
BMRBi | P08337 | |||||
SMRi | P08337 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261888, 366 interactors 849225, 4 interactors |
DIPi | DIP-10288N |
IntActi | P08337, 5 interactors |
STRINGi | 511145.b0099 |
Chemistry databases
DrugBanki | DB02023, 8-oxo-dGMP |
Proteomic databases
jPOSTi | P08337 |
PaxDbi | P08337 |
PRIDEi | P08337 |
Genome annotation databases
EnsemblBacteriai | AAC73210; AAC73210; b0099 BAB96667; BAB96667; BAB96667 |
GeneIDi | 944824 |
KEGGi | ecj:JW0097 eco:b0099 |
PATRICi | fig|1411691.4.peg.2181 |
Organism-specific databases
EchoBASEi | EB0621 |
Phylogenomic databases
eggNOGi | COG0494, Bacteria |
HOGENOMi | CLU_037162_19_2_6 |
InParanoidi | P08337 |
PhylomeDBi | P08337 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10626-MONOMER MetaCyc:EG10626-MONOMER |
BRENDAi | 3.6.1.55, 2026 |
SABIO-RKi | P08337 |
Miscellaneous databases
EvolutionaryTracei | P08337 |
PROi | PR:P08337 |
Family and domain databases
InterProi | View protein in InterPro IPR003561, Mutator_MutT IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01401, MUTATORMUTT PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
TIGRFAMsi | TIGR00586, mutt, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUTT_ECOLI | |
Accessioni | P08337Primary (citable) accession number: P08337 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families