Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase

Gene

cpdB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'-cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate.1 Publication

Miscellaneous

Two kinetically distinguishable active sites for the two substrates (2',3'-cyclic nucleotides and 3'-nucleotides) have been identified.

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate.1 Publication
A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Divalent metal cation 1By similarity1
Metal bindingi33Divalent metal cation 1By similarity1
Metal bindingi76Divalent metal cation 1By similarity1
Metal bindingi76Divalent metal cation 2By similarity1
Metal bindingi116Divalent metal cation 2By similarity1
Metal bindingi225Divalent metal cation 2By similarity1
Metal bindingi257Divalent metal cation 2By similarity1
Metal bindingi259Divalent metal cation 1By similarity1
Binding sitei440SubstrateBy similarity1

GO - Molecular functioni

  • 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: EcoCyc
  • 3'-nucleotidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • nucleotide catabolic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme
LigandMetal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CPDB-MONOMER
MetaCyc:CPDB-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC:3.1.3.6, EC:3.1.4.16)
Gene namesi
Name:cpdB
Ordered Locus Names:b4213, JW4171
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10160 cpdB

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000000003520 – 6472',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidaseAdd BLAST628

Proteomic databases

EPDiP08331
PaxDbiP08331
PRIDEiP08331

Interactioni

Protein-protein interaction databases

BioGridi4262719, 40 interactors
DIPiDIP-9311N
IntActiP08331, 2 interactors
STRINGi316385.ECDH10B_4408

Structurei

3D structure databases

ProteinModelPortaliP08331
SMRiP08331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni544 – 550Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107RNS Bacteria
COG0737 LUCA
HOGENOMiHOG000248800
InParanoidiP08331
KOiK01119
OMAiMVWDKAN

Family and domain databases

Gene3Di3.60.21.10, 1 hit
3.90.780.10, 1 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR006294 Cyc_nuc_PDE_nucleotidase
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
TIGRFAMsiTIGR01390 CycNucDiestase, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKFSATLLA TLIAASVNAA TVDLRIMETT DLHSNMMDFD YYKDTATEKF
60 70 80 90 100
GLVRTASLIN DARNEVKNSV LVDNGDLIQG SPLADYMSAK GLKAGDIHPV
110 120 130 140 150
YKALNTLDYT VGTLGNHEFN YGLDYLKNAL AGAKFPYVNA NVIDARTKQP
160 170 180 190 200
MFTPYLIKDT EVVDKDGKKQ TLKIGYIGVV PPQIMGWDKA NLSGKVTVND
210 220 230 240 250
ITETVRKYVP EMREKGADVV VVLAHSGLSA DPYKVMAENS VYYLSEIPGV
260 270 280 290 300
NAIMFGHAHA VFPGKDFADI EGADIAKGTL NGVPAVMPGM WGDHLGVVDL
310 320 330 340 350
QLSNDSGKWQ VTQAKAEARP IYDIANKKSL AAEDSKLVET LKADHDATRQ
360 370 380 390 400
FVSKPIGKSA DNMYSYLALV QDDPTVQVVN NAQKAYVEHY IQGDPDLAKL
410 420 430 440 450
PVLSAAAPFK VGGRKNDPAS YVEVEKGQLT FRNAADLYLY PNTLIVVKAS
460 470 480 490 500
GKEVKEWLEC SAGQFNQIDP NSTKPQSLIN WDGFRTYNFD VIDGVNYQID
510 520 530 540 550
VTQPARYDGE CQMINANAER IKNLTFNGKP IDPNAMFLVA TNNYRAYGGK
560 570 580 590 600
FAGTGDSHIA FASPDENRSV LAAWIADESK RAGEIHPAAD NNWRLAPIAG
610 620 630 640
DKKLDIRFET SPSDKAAAFI KEKGQYPMNK VATDDIGFAI YQVDLSK
Length:647
Mass (Da):70,832
Last modified:February 1, 1995 - v2
Checksum:i4B26DC3563473827
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316A → G in AAA23597 (PubMed:3005231).Curated1
Sequence conflicti528 – 552GKPID…GGKFA → ASRLIRTPCSWLPPITIALT GQIC in AAA23597 (PubMed:3005231).CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13464 Genomic DNA Translation: AAA23597.1
U14003 Genomic DNA Translation: AAA97109.1
U00096 Genomic DNA Translation: AAC77170.1
AP009048 Genomic DNA Translation: BAE78214.1
X54008 Genomic DNA Translation: CAA37954.1
PIRiH65232 ESECPC
RefSeqiNP_418634.1, NC_000913.3
WP_000589431.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77170; AAC77170; b4213
BAE78214; BAE78214; BAE78214
GeneIDi948729
KEGGiecj:JW4171
eco:b4213
PATRICifig|1411691.4.peg.2488

Similar proteinsi

Entry informationi

Entry nameiCPDB_ECOLI
AccessioniPrimary (citable) accession number: P08331
Secondary accession number(s): Q2M692
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health