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Protein

Alcohol dehydrogenase 4

Gene

ADH4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Zinc 1; catalytic1
Metal bindingi69Zinc 1; catalytic1
Metal bindingi99Zinc 21
Metal bindingi102Zinc 21
Metal bindingi105Zinc 21
Metal bindingi113Zinc 21
Metal bindingi180Zinc 1; catalytic1
Binding sitei229NAD1 Publication1
Binding sitei234NAD1 Publication1
Binding sitei375NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 49NAD1 Publication2
Nucleotide bindingi205 – 210NAD1 Publication6
Nucleotide bindingi298 – 300NAD1 Publication3
Nucleotide bindingi323 – 325NAD1 Publication3

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: UniProtKB
  • alcohol dehydrogenase activity, zinc-dependent Source: UniProtKB
  • alditol:NADP+ 1-oxidoreductase activity Source: Ensembl
  • all-trans retinal binding Source: UniProtKB
  • benzaldehyde dehydrogenase activity Source: UniProtKB
  • ethanol binding Source: InterPro
  • NAD binding Source: UniProtKB
  • NADPH:quinone reductase activity Source: UniProtKB
  • oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Source: UniProtKB
  • retinol binding Source: UniProtKB
  • retinol dehydrogenase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • alcohol catabolic process Source: UniProtKB
  • alcohol metabolic process Source: UniProtKB
  • cellular aldehyde metabolic process Source: UniProtKB
  • ethanol oxidation Source: UniProtKB
  • quinone metabolic process Source: UniProtKB
  • retinoid metabolic process Source: UniProtKB
  • retinol metabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS06569-MONOMER
BRENDAi1.1.1.1 2681
ReactomeiR-HSA-5365859 RA biosynthesis pathway
R-HSA-71384 Ethanol oxidation
SABIO-RKiP08319

Chemistry databases

SwissLipidsiSLP:000000499

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 4 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase class II pi chain
Gene namesi
Name:ADH4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000198099.8
HGNCiHGNC:252 ADH4
MIMi103740 gene
neXtProtiNX_P08319

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi127
OpenTargetsiENSG00000198099
PharmGKBiPA24573

Chemistry databases

ChEMBLiCHEMBL2990
DrugBankiDB03559 Cyclohexylformamide
DB00898 Ethanol
DB00157 NADH

Polymorphism and mutation databases

BioMutaiADH4
DMDMi308153684

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001606811 – 380Alcohol dehydrogenase 4Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei121PhosphoserineCombined sources1
Modified residuei278PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08319
PaxDbiP08319
PeptideAtlasiP08319
PRIDEiP08319
ProteomicsDBi52106
52107 [P08319-2]

PTM databases

iPTMnetiP08319
PhosphoSitePlusiP08319

Expressioni

Gene expression databases

BgeeiENSG00000198099
CleanExiHS_ADH4
ExpressionAtlasiP08319 baseline and differential
GenevisibleiP08319 HS

Organism-specific databases

HPAiHPA020525

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi106639, 3 interactors
IntActiP08319, 1 interactor
STRINGi9606.ENSP00000265512

Chemistry databases

BindingDBiP08319

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 15Combined sources8
Beta strandi23 – 29Combined sources7
Beta strandi36 – 45Combined sources10
Helixi48 – 52Combined sources5
Beta strandi63 – 65Combined sources3
Beta strandi70 – 78Combined sources9
Beta strandi90 – 93Combined sources4
Beta strandi100 – 102Combined sources3
Turni103 – 106Combined sources4
Helixi122 – 124Combined sources3
Beta strandi135 – 138Combined sources4
Beta strandi141 – 144Combined sources4
Turni147 – 149Combined sources3
Beta strandi152 – 159Combined sources8
Beta strandi162 – 165Combined sources4
Helixi172 – 175Combined sources4
Helixi176 – 179Combined sources4
Helixi181 – 190Combined sources10
Turni191 – 193Combined sources3
Beta strandi200 – 204Combined sources5
Helixi208 – 219Combined sources12
Beta strandi223 – 228Combined sources6
Helixi232 – 234Combined sources3
Helixi235 – 240Combined sources6
Beta strandi244 – 247Combined sources4
Helixi249 – 251Combined sources3
Helixi256 – 263Combined sources8
Beta strandi268 – 273Combined sources6
Helixi278 – 286Combined sources9
Turni290 – 292Combined sources3
Beta strandi293 – 297Combined sources5
Beta strandi307 – 309Combined sources3
Helixi311 – 315Combined sources5
Beta strandi318 – 322Combined sources5
Helixi325 – 327Combined sources3
Helixi330 – 342Combined sources13
Helixi349 – 351Combined sources3
Beta strandi352 – 357Combined sources6
Helixi358 – 360Combined sources3
Helixi361 – 369Combined sources9
Beta strandi374 – 379Combined sources6

3D structure databases

ProteinModelPortaliP08319
SMRiP08319
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08319

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiP08319
KOiK13980
OMAiMKAALDC
OrthoDBiEOG091G08N3
PhylomeDBiP08319
TreeFamiTF300429

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
IPR028632 Zinc_ADH_II
PANTHERiPTHR43880:SF14 PTHR43880:SF14, 1 hit
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P08319-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTKGKVIKC KAAIAWEAGK PLCIEEVEVA PPKAHEVRIQ IIATSLCHTD
60 70 80 90 100
ATVIDSKFEG LAFPVIVGHE AAGIVESIGP GVTNVKPGDK VIPLYAPLCR
110 120 130 140 150
KCKFCLSPLT NLCGKISNLK SPASDQQLME DKTSRFTCKG KPVYHFFGTS
160 170 180 190 200
TFSQYTVVSD INLAKIDDDA NLERVCLLGC GFSTGYGAAI NNAKVTPGST
210 220 230 240 250
CAVFGLGGVG LSAVMGCKAA GASRIIGIDI NSEKFVKAKA LGATDCLNPR
260 270 280 290 300
DLHKPIQEVI IELTKGGVDF ALDCAGGSET MKAALDCTTA GWGSCTFIGV
310 320 330 340 350
AAGSKGLTIF PEELIIGRTI NGTFFGGWKS VDSIPKLVTD YKNKKFNLDA
360 370 380
LVTHTLPFDK ISEAFDLMNQ GKSVRTILIF
Length:380
Mass (Da):40,222
Last modified:October 5, 2010 - v5
Checksum:i45721A08197629F1
GO
Isoform 2 (identifier: P08319-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MGTKGK → MLVRGPHFELQRCKTHLFSSNYLTQ

Show »
Length:399
Mass (Da):42,607
Checksum:iC4ABEC85C04B6C2D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52T → S in AAA51595 (PubMed:3036213).Curated1
Sequence conflicti52T → S in CAA39813 (PubMed:1889753).Curated1
Sequence conflicti380F → FGRCQEQFRILSD in AAA51595 (PubMed:3036213).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023461309I → V2 PublicationsCorresponds to variant dbSNP:rs1126671Ensembl.1
Natural variantiVAR_023462318R → H1 PublicationCorresponds to variant dbSNP:rs29001219Ensembl.1
Natural variantiVAR_023463374V → I2 PublicationsCorresponds to variant dbSNP:rs1126673Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0367881 – 6MGTKGK → MLVRGPHFELQRCKTHLFSS NYLTQ in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15943 mRNA Translation: AAA51595.1
X56411
, X56412, X56413, X56414, X56415, X56416, X56417, X56418, X56419 Genomic DNA Translation: CAA39813.1
AK290835 mRNA Translation: BAF83524.1
AK295556 mRNA Translation: BAG58459.1
AY974245 Genomic DNA Translation: AAX59034.1
AC019131 Genomic DNA No translation available.
AP002026 Genomic DNA No translation available.
BC022319 mRNA Translation: AAH22319.1
CCDSiCCDS34032.1 [P08319-1]
CCDS77942.1 [P08319-2]
PIRiA27109 DEHUAP
RefSeqiNP_000661.2, NM_000670.4 [P08319-1]
NP_001293100.1, NM_001306171.1 [P08319-2]
NP_001293101.1, NM_001306172.1 [P08319-2]
UniGeneiHs.1219

Genome annotation databases

EnsembliENST00000265512; ENSP00000265512; ENSG00000198099 [P08319-1]
ENST00000505590; ENSP00000425416; ENSG00000198099 [P08319-2]
ENST00000508393; ENSP00000424630; ENSG00000198099 [P08319-2]
GeneIDi127
KEGGihsa:127
UCSCiuc003hun.4 human [P08319-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADH4_HUMAN
AccessioniPrimary (citable) accession number: P08319
Secondary accession number(s): A8K470
, B4DIE7, C9J4A9, Q8TCD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 5, 2010
Last modified: June 20, 2018
This is version 188 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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