UniProtKB - P08312 (SYFA_ECOLI)
Protein
Phenylalanine--tRNA ligase alpha subunit
Gene
pheS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:6.1.1.20
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 252 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- phenylalanine-tRNA ligase activity Source: GO_Central
- tRNA binding Source: InterPro
GO - Biological processi
- phenylalanyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PHES-MONOMER MetaCyc:PHES-MONOMER |
SABIO-RKi | P08312 |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)Alternative name(s): Phenylalanyl-tRNA synthetase alpha subunit Short name: PheRS |
Gene namesi | Name:pheS Ordered Locus Names:b1714, JW5277 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: UniProtKB
- phenylalanine-tRNA ligase complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126701 | 1 – 327 | Phenylalanine--tRNA ligase alpha subunitAdd BLAST | 327 |
Proteomic databases
jPOSTi | P08312 |
PaxDbi | P08312 |
PRIDEi | P08312 |
2D gel databases
SWISS-2DPAGEi | P08312 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta subunits.
Binary interactionsi
Hide detailsP08312
With | #Exp. | IntAct |
---|---|---|
brnQ [P0AD99] | 5 | EBI-555676,EBI-555687 |
erfK [P39176] | 4 | EBI-555676,EBI-555707 |
pheT [P07395] | 9 | EBI-555676,EBI-555713 |
Protein-protein interaction databases
BioGRIDi | 4260287, 264 interactors 850583, 8 interactors |
ComplexPortali | CPX-5222, Phenylalanyl-tRNA synthetase complex |
DIPi | DIP-6878N |
IntActi | P08312, 7 interactors |
STRINGi | 511145.b1714 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08312 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.Curated
Phylogenomic databases
eggNOGi | COG0016, Bacteria |
HOGENOMi | CLU_025086_0_1_6 |
InParanoidi | P08312 |
PhylomeDBi | P08312 |
Family and domain databases
HAMAPi | MF_00281, Phe_tRNA_synth_alpha1, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004529, Phe-tRNA-synth_IIc_asu IPR004530, Phe-tRNA-synth_IIc_mito IPR004188, Phe-tRNA_ligase_II_N IPR022911, Phe_tRNA_ligase_alpha1_bac IPR002319, Phenylalanyl-tRNA_Synthase IPR010978, tRNA-bd_arm |
PANTHERi | PTHR11538:SF41, PTHR11538:SF41, 1 hit |
Pfami | View protein in Pfam PF02912, Phe_tRNA-synt_N, 1 hit PF01409, tRNA-synt_2d, 1 hit |
SUPFAMi | SSF46589, SSF46589, 1 hit |
TIGRFAMsi | TIGR00468, pheS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P08312-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHLAELVAS AKAAISQASD VAALDNVRVE YLGKKGHLTL QMTTLRELPP
60 70 80 90 100
EERPAAGAVI NEAKEQVQQA LNARKAELES AALNARLAAE TIDVSLPGRR
110 120 130 140 150
IENGGLHPVT RTIDRIESFF GELGFTVATG PEIEDDYHNF DALNIPGHHP
160 170 180 190 200
ARADHDTFWF DTTRLLRTQT SGVQIRTMKA QQPPIRIIAP GRVYRNDYDQ
210 220 230 240 250
THTPMFHQME GLIVDTNISF TNLKGTLHDF LRNFFEEDLQ IRFRPSYFPF
260 270 280 290 300
TEPSAEVDVM GKNGKWLEVL GCGMVHPNVL RNVGIDPEVY SGFAFGMGME
310 320
RLTMLRYGVT DLRSFFENDL RFLKQFK
Sequence cautioni
The sequence CAA23564 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | R → A in CAA23564 (Ref. 2) Curated | 1 | |
Sequence conflicti | 74 | R → A in AAA51469 (PubMed:9278503).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 98 | G → D in thermosensitive mutant pheS5; might cause subunit disaggregation due to electrostatic repulsion. 1 Publication | 1 | |
Natural varianti | 191 | G → D Decreased affinity for Phe. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00291 Genomic DNA Translation: CAA23564.1 Different initiation. K02844 Genomic DNA Translation: AAA51469.1 U00096 Genomic DNA Translation: AAC74784.1 AP009048 Genomic DNA Translation: BAA15482.2 |
PIRi | B64930, SYECFA |
RefSeqi | NP_416229.1, NC_000913.3 WP_000018596.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74784; AAC74784; b1714 BAA15482; BAA15482; BAA15482 |
GeneIDi | 57731111 946223 |
KEGGi | ecj:JW5277 eco:b1714 |
PATRICi | fig|1411691.4.peg.543 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00291 Genomic DNA Translation: CAA23564.1 Different initiation. K02844 Genomic DNA Translation: AAA51469.1 U00096 Genomic DNA Translation: AAC74784.1 AP009048 Genomic DNA Translation: BAA15482.2 |
PIRi | B64930, SYECFA |
RefSeqi | NP_416229.1, NC_000913.3 WP_000018596.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3PCO | X-ray | 3.02 | A/C | 1-327 | [»] | |
6P24 | X-ray | 2.12 | A/C | 2-327 | [»] | |
SMRi | P08312 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260287, 264 interactors 850583, 8 interactors |
ComplexPortali | CPX-5222, Phenylalanyl-tRNA synthetase complex |
DIPi | DIP-6878N |
IntActi | P08312, 7 interactors |
STRINGi | 511145.b1714 |
Chemistry databases
DrugCentrali | P08312 |
2D gel databases
SWISS-2DPAGEi | P08312 |
Proteomic databases
jPOSTi | P08312 |
PaxDbi | P08312 |
PRIDEi | P08312 |
Genome annotation databases
EnsemblBacteriai | AAC74784; AAC74784; b1714 BAA15482; BAA15482; BAA15482 |
GeneIDi | 57731111 946223 |
KEGGi | ecj:JW5277 eco:b1714 |
PATRICi | fig|1411691.4.peg.543 |
Organism-specific databases
EchoBASEi | EB0703 |
Phylogenomic databases
eggNOGi | COG0016, Bacteria |
HOGENOMi | CLU_025086_0_1_6 |
InParanoidi | P08312 |
PhylomeDBi | P08312 |
Enzyme and pathway databases
BioCyci | EcoCyc:PHES-MONOMER MetaCyc:PHES-MONOMER |
SABIO-RKi | P08312 |
Miscellaneous databases
PROi | PR:P08312 |
Family and domain databases
HAMAPi | MF_00281, Phe_tRNA_synth_alpha1, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004529, Phe-tRNA-synth_IIc_asu IPR004530, Phe-tRNA-synth_IIc_mito IPR004188, Phe-tRNA_ligase_II_N IPR022911, Phe_tRNA_ligase_alpha1_bac IPR002319, Phenylalanyl-tRNA_Synthase IPR010978, tRNA-bd_arm |
PANTHERi | PTHR11538:SF41, PTHR11538:SF41, 1 hit |
Pfami | View protein in Pfam PF02912, Phe_tRNA-synt_N, 1 hit PF01409, tRNA-synt_2d, 1 hit |
SUPFAMi | SSF46589, SSF46589, 1 hit |
TIGRFAMsi | TIGR00468, pheS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYFA_ECOLI | |
Accessioni | P08312Primary (citable) accession number: P08312 Secondary accession number(s): P78233, Q59406 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families