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Protein

Cathepsin G

Gene

CTSG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.3 Publications

Catalytic activityi

Specificity similar to chymotrypsin C.

Activity regulationi

Inhibited by soybean trypsin inhibitor, benzamidine, the synthetic peptide R13K, Z-Gly-Leu-Phe-CH2Cl, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, DFP, SBTI and alpha-1-antitrypsin. Inhibited by LPS from P.aeruginosa but not by LPS from S.minnesota. Not inhibited by elastinal, CMK, TLCK and ETDA.3 Publications

Kineticsi

  1. KM=1.15 mM for Z-Lys-SBzl1 Publication
  2. KM=0.26 mM for Suc-Ala-Ala-Pro-Phe-SBzl1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei64Charge relay system1
    Active sitei108Charge relay system1
    Active sitei201Charge relay system1

    GO - Molecular functioni

    • heparin binding Source: MGI
    • peptidase activity Source: MGI
    • serine-type endopeptidase activity Source: UniProtKB
    • serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionAntibiotic, Antimicrobial, Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.20 2681
    ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
    R-HSA-1592389 Activation of Matrix Metalloproteinases
    R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
    R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
    R-HSA-6798695 Neutrophil degranulation
    R-HSA-6803157 Antimicrobial peptides
    SABIO-RKiP08311

    Protein family/group databases

    MEROPSiS01.133

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cathepsin G (EC:3.4.21.20)
    Short name:
    CG
    Gene namesi
    Name:CTSG
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 14

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000100448.3
    HGNCiHGNC:2532 CTSG
    MIMi116830 gene
    neXtProtiNX_P08311

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi1511
    OpenTargetsiENSG00000100448
    PharmGKBiPA27032

    Chemistry databases

    ChEMBLiCHEMBL4071
    DrugBankiDB04016 2-[3-({Methyl[1-(2-Naphthoyl)Piperidin-4-Yl]Amino}Carbonyl)-2-Naphthyl]-1-(1-Naphthyl)-2-Oxoethylphosphonic Acid
    DB02360 Bis-Napthyl Beta-Ketophosphonic Acid
    GuidetoPHARMACOLOGYi2348

    Polymorphism and mutation databases

    BioMutaiCTSG
    DMDMi115725

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 18Add BLAST18
    PropeptideiPRO_000002751219 – 20Activation peptide5 Publications2
    ChainiPRO_000002751321 – 255Cathepsin GAdd BLAST235

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi49 ↔ 65
    Glycosylationi71N-linked (GlcNAc...) asparagine1
    Disulfide bondi142 ↔ 207
    Disulfide bondi172 ↔ 186

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    EPDiP08311
    MaxQBiP08311
    PaxDbiP08311
    PeptideAtlasiP08311
    PRIDEiP08311
    ProteomicsDBi12623
    52105
    TopDownProteomicsiP08311

    PTM databases

    iPTMnetiP08311
    PhosphoSitePlusiP08311

    Miscellaneous databases

    PMAP-CutDBiP08311

    Expressioni

    Gene expression databases

    BgeeiENSG00000100448 Expressed in 151 organ(s), highest expression level in bone marrow
    CleanExiHS_CTSG
    GenevisibleiP08311 HS

    Organism-specific databases

    HPAiCAB000110
    HPA047737

    Interactioni

    Subunit structurei

    (Microbial infection) Interacts with M.tuberculosis protein Rv3364c.1 Publication

    Binary interactionsi

    Protein-protein interaction databases

    BioGridi107891, 17 interactors
    CORUMiP08311
    IntActiP08311, 6 interactors
    MINTiP08311
    STRINGi9606.ENSP00000216336

    Chemistry databases

    BindingDBiP08311

    Structurei

    Secondary structure

    1255
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP08311
    SMRiP08311
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08311

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini21 – 243Peptidase S1PROSITE-ProRule annotationAdd BLAST223

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG3627 Eukaryota
    COG5640 LUCA
    GeneTreeiENSGT00760000118895
    HOGENOMiHOG000251820
    HOVERGENiHBG013304
    InParanoidiP08311
    KOiK01319
    OMAiICVGDRR
    OrthoDBiEOG091G0G5F
    PhylomeDBiP08311
    TreeFamiTF333630

    Family and domain databases

    CDDicd00190 Tryp_SPc, 1 hit
    InterProiView protein in InterPro
    IPR009003 Peptidase_S1_PA
    IPR001314 Peptidase_S1A
    IPR001254 Trypsin_dom
    IPR018114 TRYPSIN_HIS
    IPR033116 TRYPSIN_SER
    PfamiView protein in Pfam
    PF00089 Trypsin, 1 hit
    PRINTSiPR00722 CHYMOTRYPSIN
    SMARTiView protein in SMART
    SM00020 Tryp_SPc, 1 hit
    SUPFAMiSSF50494 SSF50494, 1 hit
    PROSITEiView protein in PROSITE
    PS50240 TRYPSIN_DOM, 1 hit
    PS00134 TRYPSIN_HIS, 1 hit
    PS00135 TRYPSIN_SER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08311-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQPLLLLLAF LLPTGAEAGE IIGGRESRPH SRPYMAYLQI QSPAGQSRCG
    60 70 80 90 100
    GFLVREDFVL TAAHCWGSNI NVTLGAHNIQ RRENTQQHIT ARRAIRHPQY
    110 120 130 140 150
    NQRTIQNDIM LLQLSRRVRR NRNVNPVALP RAQEGLRPGT LCTVAGWGRV
    160 170 180 190 200
    SMRRGTDTLR EVQLRVQRDR QCLRIFGSYD PRRQICVGDR RERKAAFKGD
    210 220 230 240 250
    SGGPLLCNNV AHGIVSYGKS SGVPPEVFTR VSSFLPWIRT TMRSFKLLDQ

    METPL
    Length:255
    Mass (Da):28,837
    Last modified:January 1, 1990 - v2
    Checksum:i6228E741E6A43889
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_006491125N → S1 PublicationCorresponds to variant dbSNP:rs45567233EnsemblClinVar.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16117 mRNA Translation: AAA52126.1
    J04990 Genomic DNA Translation: AAA51919.1
    CR456807 mRNA Translation: CAG33088.1
    CR541704 mRNA Translation: CAG46505.1
    CH471078 Genomic DNA Translation: EAW66006.1
    BC014460 mRNA Translation: AAH14460.1
    CCDSiCCDS9631.1
    PIRiA32627 A27122
    RefSeqiNP_001902.1, NM_001911.2
    UniGeneiHs.421724

    Genome annotation databases

    EnsembliENST00000216336; ENSP00000216336; ENSG00000100448
    GeneIDi1511
    KEGGihsa:1511
    UCSCiuc001wpq.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16117 mRNA Translation: AAA52126.1
    J04990 Genomic DNA Translation: AAA51919.1
    CR456807 mRNA Translation: CAG33088.1
    CR541704 mRNA Translation: CAG46505.1
    CH471078 Genomic DNA Translation: EAW66006.1
    BC014460 mRNA Translation: AAH14460.1
    CCDSiCCDS9631.1
    PIRiA32627 A27122
    RefSeqiNP_001902.1, NM_001911.2
    UniGeneiHs.421724

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1AU8X-ray1.90A21-244[»]
    1CGHX-ray1.80A21-244[»]
    1KYNX-ray3.50A/B21-255[»]
    1T32X-ray1.85A21-244[»]
    ProteinModelPortaliP08311
    SMRiP08311
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi107891, 17 interactors
    CORUMiP08311
    IntActiP08311, 6 interactors
    MINTiP08311
    STRINGi9606.ENSP00000216336

    Chemistry databases

    BindingDBiP08311
    ChEMBLiCHEMBL4071
    DrugBankiDB04016 2-[3-({Methyl[1-(2-Naphthoyl)Piperidin-4-Yl]Amino}Carbonyl)-2-Naphthyl]-1-(1-Naphthyl)-2-Oxoethylphosphonic Acid
    DB02360 Bis-Napthyl Beta-Ketophosphonic Acid
    GuidetoPHARMACOLOGYi2348

    Protein family/group databases

    MEROPSiS01.133

    PTM databases

    iPTMnetiP08311
    PhosphoSitePlusiP08311

    Polymorphism and mutation databases

    BioMutaiCTSG
    DMDMi115725

    Proteomic databases

    EPDiP08311
    MaxQBiP08311
    PaxDbiP08311
    PeptideAtlasiP08311
    PRIDEiP08311
    ProteomicsDBi12623
    52105
    TopDownProteomicsiP08311

    Protocols and materials databases

    DNASUi1511
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000216336; ENSP00000216336; ENSG00000100448
    GeneIDi1511
    KEGGihsa:1511
    UCSCiuc001wpq.4 human

    Organism-specific databases

    CTDi1511
    DisGeNETi1511
    EuPathDBiHostDB:ENSG00000100448.3
    GeneCardsiCTSG
    HGNCiHGNC:2532 CTSG
    HPAiCAB000110
    HPA047737
    MIMi116830 gene
    neXtProtiNX_P08311
    OpenTargetsiENSG00000100448
    PharmGKBiPA27032
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3627 Eukaryota
    COG5640 LUCA
    GeneTreeiENSGT00760000118895
    HOGENOMiHOG000251820
    HOVERGENiHBG013304
    InParanoidiP08311
    KOiK01319
    OMAiICVGDRR
    OrthoDBiEOG091G0G5F
    PhylomeDBiP08311
    TreeFamiTF333630

    Enzyme and pathway databases

    BRENDAi3.4.21.20 2681
    ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
    R-HSA-1592389 Activation of Matrix Metalloproteinases
    R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
    R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
    R-HSA-6798695 Neutrophil degranulation
    R-HSA-6803157 Antimicrobial peptides
    SABIO-RKiP08311

    Miscellaneous databases

    EvolutionaryTraceiP08311
    GeneWikiiCathepsin_G
    GenomeRNAii1511
    PMAP-CutDBiP08311
    PROiPR:P08311
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000100448 Expressed in 151 organ(s), highest expression level in bone marrow
    CleanExiHS_CTSG
    GenevisibleiP08311 HS

    Family and domain databases

    CDDicd00190 Tryp_SPc, 1 hit
    InterProiView protein in InterPro
    IPR009003 Peptidase_S1_PA
    IPR001314 Peptidase_S1A
    IPR001254 Trypsin_dom
    IPR018114 TRYPSIN_HIS
    IPR033116 TRYPSIN_SER
    PfamiView protein in Pfam
    PF00089 Trypsin, 1 hit
    PRINTSiPR00722 CHYMOTRYPSIN
    SMARTiView protein in SMART
    SM00020 Tryp_SPc, 1 hit
    SUPFAMiSSF50494 SSF50494, 1 hit
    PROSITEiView protein in PROSITE
    PS50240 TRYPSIN_DOM, 1 hit
    PS00134 TRYPSIN_HIS, 1 hit
    PS00135 TRYPSIN_SER, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCATG_HUMAN
    AccessioniPrimary (citable) accession number: P08311
    Secondary accession number(s): Q6IBJ6, Q9UCA5, Q9UCU6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 1, 1990
    Last modified: September 12, 2018
    This is version 183 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    6. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    7. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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