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UniProtKB - P08310 (CATB_PSEPU)
Protein
Muconate cycloisomerase 1
Gene
catB
Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Functioni
Catalyzes a syn cycloisomerization.
Catalytic activityi
- EC:5.5.1.11 Publication
Cofactori
: beta-ketoadipate pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol. This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 171 | Proton acceptor | 1 | |
Metal bindingi | 200 | Manganese | 1 | |
Metal bindingi | 226 | Manganese | 1 | |
Metal bindingi | 251 | Manganese | 1 | |
Active sitei | 329 | Proton donor | 1 |
GO - Molecular functioni
- chloromuconate cycloisomerase activity Source: InterPro
- manganese ion binding Source: InterPro
- muconate cycloisomerase activity Source: UniProtKB-EC
GO - Biological processi
- beta-ketoadipate pathway Source: UniProtKB-UniPathway
- cellular amino acid catabolic process Source: InterPro
Keywordsi
Molecular function | Isomerase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 5.5.1.1, 5092 |
SABIO-RKi | P08310 |
UniPathwayi | UPA00157;UER00259 |
Names & Taxonomyi
Protein namesi | Recommended name: Muconate cycloisomerase 1 (EC:5.5.1.11 Publication)Alternative name(s): Cis,cis-muconate lactonizing enzyme I Short name: MLE Muconate cycloisomerase I |
Gene namesi | Name:catB |
Organismi | Pseudomonas putida (Arthrobacter siderocapsulatus) |
Taxonomic identifieri | 303 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000171251 | 2 – 375 | Muconate cycloisomerase 1Add BLAST | 374 |
Proteomic databases
PRIDEi | P08310 |
Interactioni
Subunit structurei
Homooctamer.
Protein-protein interaction databases
STRINGi | 1240350.AMZE01000026_gene4644 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08310 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08310 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mandelate racemase/muconate lactonizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
Family and domain databases
CDDi | cd03318, MLE, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR013370, Chloromuconate_cycloisomerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDG01258, (chloro)muconate_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR02534, mucon_cyclo, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P08310-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSVLIERIE AIIVHDLPTI RPPHKLAMHT MQTQTLVLIR VRCSDGVEGI
60 70 80 90 100
GEATTIGGLA YGYESPEGIK ANIDAHLAPA LVGLPADNIN AAMLKLDKLA
110 120 130 140 150
KGNTFAKSGI ESALLDAQGK RLGLPVSELL GGRVRDSLEV AWTLASGDTA
160 170 180 190 200
RDIAEAQHML EIRRHRVFKL KIGANPLAQD LKHVVAIKRE LGDSASVRVD
210 220 230 240 250
VNQYWDESQA IRACQVLGDN GIDLIEQPIS RINRSGQVRL NQRSPAPIMA
260 270 280 290 300
DESIESVEDA FSLAADGAAS IFALKIAKNG GPRAVLRTAQ IAEAAGIALY
310 320 330 340 350
GGTMLEGSIG TLASAHAFLT LRQLTWGTEL FGPLLLTEEI VNEPPQYRDF
360 370
QLHIPRTPGL GLTLDEQRLA RFARR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | T → S in AAA25766 (PubMed:2449420).Curated | 1 | |
Sequence conflicti | 65 | S → T in AAA25766 (PubMed:2449420).Curated | 1 | |
Sequence conflicti | 244 | Missing in AAA25765 (PubMed:3609743).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19460 Genomic DNA Translation: AAA25766.1 M16236 Genomic DNA Translation: AAA25765.1 |
PIRi | A28630 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19460 Genomic DNA Translation: AAA25766.1 M16236 Genomic DNA Translation: AAA25765.1 |
PIRi | A28630 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MUC | X-ray | 1.85 | A/B | 1-375 | [»] | |
2MUC | X-ray | 2.30 | A/B | 1-375 | [»] | |
3MUC | X-ray | 2.30 | A/B | 5-374 | [»] | |
SMRi | P08310 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 1240350.AMZE01000026_gene4644 |
Proteomic databases
PRIDEi | P08310 |
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
Enzyme and pathway databases
UniPathwayi | UPA00157;UER00259 |
BRENDAi | 5.5.1.1, 5092 |
SABIO-RKi | P08310 |
Miscellaneous databases
EvolutionaryTracei | P08310 |
Family and domain databases
CDDi | cd03318, MLE, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR013370, Chloromuconate_cycloisomerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDG01258, (chloro)muconate_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR02534, mucon_cyclo, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CATB_PSEPU | |
Accessioni | P08310Primary (citable) accession number: P08310 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families