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Protein

Glutathione S-transferase A1

Gene

GSTA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9Glutathione2 Publications1
Binding sitei45Glutathione2 Publications1

GO - Molecular functioni

  • glutathione peroxidase activity Source: BHF-UCL
  • glutathione transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:G66-32542-MONOMER
BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKiP08263
SIGNORiP08263

Chemistry databases

SwissLipidsiSLP:000001476

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A1 (EC:2.5.1.18)
Alternative name(s):
GST HA subunit 1
GST class-alpha member 1
GST-epsilon
GSTA1-1
GTH1
Cleaved into the following chain:
Gene namesi
Name:GSTA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000243955.5
HGNCiHGNC:4626 GSTA1
MIMi138359 gene
neXtProtiNX_P08263

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71I → A or V: No significant effect on enzyme activity. Reduces protein stability. 1 Publication1
Mutagenesisi216A → H: Confers ability to hydrolyze S-glutathionyl benzoate to glutathione and benzoic acid. 1 Publication1

Organism-specific databases

DisGeNETi2938
OpenTargetsiENSG00000243955
PharmGKBiPA29016

Chemistry databases

ChEMBLiCHEMBL3409
DrugBankiDB02486 2-Hydroxyethyl Disulfide
DB00993 Azathioprine
DB01008 Busulfan
DB00143 Glutathione
DB03003 Glutathione Sulfonic Acid
DB02943 N-(4-Aminobutanoyl)-S-(4-Methoxybenzyl)-L-Cysteinylglycine
DB03602 S-Benzyl-Glutathione
DB04132 S-Hexylglutathione
DB01915 S-Hydroxycysteine

Polymorphism and mutation databases

BioMutaiGSTA1
DMDMi121730

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232031 – 222Glutathione S-transferase A1Add BLAST222
Initiator methionineiRemoved; alternateBy similarity1 Publication
ChainiPRO_00001857832 – 222Glutathione S-transferase A1, N-terminally processedAdd BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in Glutathione S-transferase A1, N-terminally processedBy similarity1
Modified residuei4N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP08263
PaxDbiP08263
PeptideAtlasiP08263
PRIDEiP08263
ProteomicsDBi12637
52103

2D gel databases

REPRODUCTION-2DPAGEiIPI00657682

PTM databases

iPTMnetiP08263
PhosphoSitePlusiP08263

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiENSG00000243955 Expressed in 143 organ(s), highest expression level in nephron tubule
CleanExiHS_GSTA1
ExpressionAtlasiP08263 baseline and differential
GenevisibleiP08263 HS

Organism-specific databases

HPAiHPA004342
HPA048934
HPA053817

Interactioni

Subunit structurei

Homodimer or heterodimer of GSTA1 and GSTA2.8 Publications

Protein-protein interaction databases

BioGridi109193, 15 interactors
IntActiP08263, 6 interactors
STRINGi9606.ENSP00000335620

Chemistry databases

BindingDBiP08263

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08263
SMRiP08263
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08263

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione binding2 Publications2
Regioni67 – 68Glutathione binding2 Publications2

Domaini

The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated

Phylogenomic databases

eggNOGiKOG1695 Eukaryota
ENOG4111VAU LUCA
GeneTreeiENSGT00670000097856
HOGENOMiHOG000115734
HOVERGENiHBG053749
InParanoidiP08263
KOiK00799
OMAiNEMIIVL
OrthoDBiEOG091G0O3D
PhylomeDBiP08263
TreeFamiTF105321

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR003080 GST_alpha
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01266 GSTRNSFRASEA
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08263-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA
110 120 130 140 150
DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP
210 220
GSPRKPPMDE KSLEEARKIF RF
Length:222
Mass (Da):25,631
Last modified:January 23, 2007 - v3
Checksum:iC8B6786DCD761350
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18S → C in AAA36174 (PubMed:3678589).Curated1
Sequence conflicti51M → T in AAT06769 (Ref. 7) Curated1
Sequence conflicti56 – 58PMV → AML in AAA36174 (PubMed:3678589).Curated3
Sequence conflicti212S → L AA sequence (PubMed:2604726).Curated1
Sequence conflicti216A → S AA sequence (PubMed:8431482).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03397819T → I. Corresponds to variant dbSNP:rs1051578Ensembl.1
Natural variantiVAR_049482113P → Q. Corresponds to variant dbSNP:rs1051745Ensembl.1
Natural variantiVAR_049483117K → Q. Corresponds to variant dbSNP:rs1051757Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15872 mRNA Translation: AAA70226.1
M21758 mRNA Translation: AAA52615.1
M25627 mRNA Translation: AAA36174.1
M14777 mRNA Translation: AAA52618.1
S76235
, S76221, S76223, S76225, S76228, S76232 Genomic DNA Translation: AAB20973.1
S49975 mRNA Translation: AAB24012.1
AY532928 mRNA Translation: AAT06769.1
CR407656 mRNA Translation: CAG28584.1
AL590363 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04385.1
BC053578 mRNA Translation: AAH53578.1
BC110891 mRNA Translation: AAI10892.1
CCDSiCCDS4945.1
PIRiA25909 A56666
S29657
RefSeqiNP_665683.1, NM_145740.4
UniGeneiHs.446309

Genome annotation databases

EnsembliENST00000334575; ENSP00000335620; ENSG00000243955
GeneIDi2938
KEGGihsa:2938
UCSCiuc003paz.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15872 mRNA Translation: AAA70226.1
M21758 mRNA Translation: AAA52615.1
M25627 mRNA Translation: AAA36174.1
M14777 mRNA Translation: AAA52618.1
S76235
, S76221, S76223, S76225, S76228, S76232 Genomic DNA Translation: AAB20973.1
S49975 mRNA Translation: AAB24012.1
AY532928 mRNA Translation: AAT06769.1
CR407656 mRNA Translation: CAG28584.1
AL590363 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04385.1
BC053578 mRNA Translation: AAH53578.1
BC110891 mRNA Translation: AAI10892.1
CCDSiCCDS4945.1
PIRiA25909 A56666
S29657
RefSeqiNP_665683.1, NM_145740.4
UniGeneiHs.446309

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GSDX-ray2.50A/B/C/D2-222[»]
1GSEX-ray2.00A/B2-222[»]
1GSFX-ray2.70A/B/C/D2-222[»]
1GUHX-ray2.60A/B/C/D2-222[»]
1K3LX-ray1.50A/B2-222[»]
1K3OX-ray1.80A/B2-222[»]
1K3YX-ray1.30A/B2-222[»]
1LBKX-ray1.86A/B208-213[»]
1PKWX-ray2.00A/B1-222[»]
1PKZX-ray2.10A/B1-222[»]
1PL1X-ray1.75A/B1-222[»]
1PL2X-ray1.80A/B1-222[»]
1USBX-ray2.07A/B2-222[»]
1XWGX-ray1.85A/B2-222[»]
1YDKX-ray1.95A/B1-222[»]
2R3XX-ray1.80A/B1-222[»]
2R6KX-ray2.51A/B1-222[»]
3I69X-ray2.38A/B/C/D/E/F/G/H1-222[»]
3I6AX-ray1.98A/B/C/D/E/F/G/H1-222[»]
3IK9X-ray2.20A/B/C/D/E/F/G/H1-222[»]
3KTLX-ray1.75A/B2-222[»]
3L0HX-ray2.13A/B1-222[»]
3Q74X-ray1.79A/B2-222[»]
3U6VX-ray2.20A/B1-222[»]
3ZFBX-ray1.86A/B1-222[»]
3ZFLX-ray1.88A/B1-222[»]
4HJ2X-ray2.10A/B4-220[»]
5JCUX-ray1.93A/B/C/D2-222[»]
5LCZX-ray2.33A/B1-100[»]
A/B201-222[»]
5LD0X-ray1.60A1-85[»]
A214-222[»]
6ATOX-ray1.55A/B2-222[»]
6ATPX-ray1.70A/B2-222[»]
6ATQX-ray2.00A/B2-222[»]
6ATRX-ray1.29A/B2-222[»]
ProteinModelPortaliP08263
SMRiP08263
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109193, 15 interactors
IntActiP08263, 6 interactors
STRINGi9606.ENSP00000335620

Chemistry databases

BindingDBiP08263
ChEMBLiCHEMBL3409
DrugBankiDB02486 2-Hydroxyethyl Disulfide
DB00993 Azathioprine
DB01008 Busulfan
DB00143 Glutathione
DB03003 Glutathione Sulfonic Acid
DB02943 N-(4-Aminobutanoyl)-S-(4-Methoxybenzyl)-L-Cysteinylglycine
DB03602 S-Benzyl-Glutathione
DB04132 S-Hexylglutathione
DB01915 S-Hydroxycysteine
SwissLipidsiSLP:000001476

PTM databases

iPTMnetiP08263
PhosphoSitePlusiP08263

Polymorphism and mutation databases

BioMutaiGSTA1
DMDMi121730

2D gel databases

REPRODUCTION-2DPAGEiIPI00657682

Proteomic databases

MaxQBiP08263
PaxDbiP08263
PeptideAtlasiP08263
PRIDEiP08263
ProteomicsDBi12637
52103

Protocols and materials databases

DNASUi2938
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334575; ENSP00000335620; ENSG00000243955
GeneIDi2938
KEGGihsa:2938
UCSCiuc003paz.4 human

Organism-specific databases

CTDi2938
DisGeNETi2938
EuPathDBiHostDB:ENSG00000243955.5
GeneCardsiGSTA1
HGNCiHGNC:4626 GSTA1
HPAiHPA004342
HPA048934
HPA053817
MIMi138359 gene
neXtProtiNX_P08263
OpenTargetsiENSG00000243955
PharmGKBiPA29016
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1695 Eukaryota
ENOG4111VAU LUCA
GeneTreeiENSGT00670000097856
HOGENOMiHOG000115734
HOVERGENiHBG053749
InParanoidiP08263
KOiK00799
OMAiNEMIIVL
OrthoDBiEOG091G0O3D
PhylomeDBiP08263
TreeFamiTF105321

Enzyme and pathway databases

BioCyciMetaCyc:G66-32542-MONOMER
BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKiP08263
SIGNORiP08263

Miscellaneous databases

ChiTaRSiGSTA1 human
EvolutionaryTraceiP08263
GeneWikiiGlutathione_S-transferase_A1
GenomeRNAii2938
PROiPR:P08263
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000243955 Expressed in 143 organ(s), highest expression level in nephron tubule
CleanExiHS_GSTA1
ExpressionAtlasiP08263 baseline and differential
GenevisibleiP08263 HS

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR003080 GST_alpha
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01266 GSTRNSFRASEA
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA1_HUMAN
AccessioniPrimary (citable) accession number: P08263
Secondary accession number(s): Q14750, Q5GHF8, Q5SZC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 201 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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