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Protein

5-aminolevulinate synthase

Gene

hemA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase (hemA)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21SubstrateBy similarity1
Binding sitei136SubstrateBy similarity1
Binding sitei188Pyridoxal phosphateBy similarity1
Binding sitei216Pyridoxal phosphateBy similarity1
Binding sitei244Pyridoxal phosphateBy similarity1
Active sitei247By similarity1
Binding sitei276Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei277Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei362SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processHeme biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-1210-MONOMER
UniPathwayiUPA00251; UER00375

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:hemA
Ordered Locus Names:bll1200
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638251 – 4095-aminolevulinate synthaseAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei247N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224911.bll1200

Structurei

3D structure databases

ProteinModelPortaliP08262
SMRiP08262
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK Bacteria
COG0156 LUCA
HOGENOMiHOG000221020
InParanoidiP08262
KOiK00643
OMAiYPVIPKG
OrthoDBiPOG091H024U
PhylomeDBiP08262

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR010961 4pyrrol_synth_NH2levulA_synth
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01821 5aminolev_synth, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

P08262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSQFFNSA LDRLHTERRY RVFADLERMA GRFPHAIWHS PKGKRDVVIW
60 70 80 90 100
CSNDYLGMGQ HPKVVGAMVE TATRVGTGAG GTRNIAGTHH PLVQLEAELA
110 120 130 140 150
DLHGKEAALL FTSGYVSNQT GIATIAKLIP NCLILSDELN HNSMIEGIRQ
160 170 180 190 200
SGCERQVFRH NDLADLEALL KAAGANRPKL IACESLYSMD GDVAPLAKIC
210 220 230 240 250
DLAEKYNAMT YVDEVHAVGM YGPRGGGIAE RDGVMHRIDI LEGTLAKAFG
260 270 280 290 300
CLGGYIAANG QIIDAVRSYA PGFIFTTALP PAICSAATAA IKHLKTSSWE
310 320 330 340 350
RERHQDRAAR VKAILNAAGL PVMSSDTHIV PLFIGDAEKC KQASDLLLEE
360 370 380 390 400
HGIYIQPINY PTVAKGSERL RITPSPYHDD GLIDQLAEAL LQVWDRLGLP

LKQKSLAAE
Length:409
Mass (Da):44,574
Last modified:February 28, 2003 - v2
Checksum:iB21B011EB9542D66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261Q → R in AAA26216 (PubMed:3609750).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16751 Genomic DNA Translation: AAA26216.1
BA000040 Genomic DNA Translation: BAC46465.1
PIRiA27478 SYZJAL
RefSeqiNP_767840.1, NC_004463.1
WP_011084018.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC46465; BAC46465; BAC46465
GeneIDi1049580
KEGGibja:bll1200
PATRICifig|224911.44.peg.602

Similar proteinsi

Entry informationi

Entry nameiHEM1_BRADU
AccessioniPrimary (citable) accession number: P08262
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 28, 2003
Last modified: February 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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