Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endochitinase A

Gene

CHN48

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Defense against chitin-containing fungal pathogens.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase A (EC:3.2.1.14)
Short name:
CHN-A
Gene namesi
Name:CHN48
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000533024 – 322Endochitinase AAdd BLAST299
PropeptideiPRO_0000005331323 – 329Removed in mature form7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 41PROSITE-ProRule annotation
Disulfide bondi35 ↔ 47PROSITE-ProRule annotation
Disulfide bondi40 ↔ 54PROSITE-ProRule annotation
Disulfide bondi59 ↔ 63PROSITE-ProRule annotation
Modified residuei674-hydroxyproline; partial1 Publication1
Modified residuei694-hydroxyproline1 Publication1
Modified residuei714-hydroxyproline1 Publication1
Modified residuei724-hydroxyproline1 Publication1
Modified residuei744-hydroxyproline1 Publication1
Modified residuei754-hydroxyproline; partial1 Publication1
Disulfide bondi101 ↔ 163PROSITE-ProRule annotation
Disulfide bondi175 ↔ 183PROSITE-ProRule annotation
Disulfide bondi282 ↔ 314PROSITE-ProRule annotation

Post-translational modificationi

The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PRIDEiP08252
ProMEXiP08252

Expressioni

Developmental stagei

Expressed during flower formation.1 Publication

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliP08252
SMRiP08252
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 65Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20547

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08252-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLCKFTALS SLLFSLLLLS ASAEQCGSQA GGARCPSGLC CSKFGWCGNT
60 70 80 90 100
NDYCGPGNCQ SQCPGGPTPT PPTPPGGGDL GSIISSSMFD QMLKHRNDNA
110 120 130 140 150
CQGKGFYSYN AFINAARSFP GFGTSGDTTA RKREIAAFFA QTSHETTGGW
160 170 180 190 200
ATAPDGPYAW GYCWLREQGS PGDYCTPSGQ WPCAPGRKYF GRGPIQISHN
210 220 230 240 250
YNYGPCGRAI GVDLLNNPDL VATDPVISFK SALWFWMTPQ SPKPSCHDVI
260 270 280 290 300
IGRWQPSAGD RAANRLPGFG VITNIINGGL ECGRGTDSRV QDRIGFYRRY
310 320
CSILGVSPGD NLDCGNQRSF GNGLLVDTM
Length:329
Mass (Da):35,156
Last modified:August 1, 1990 - v2
Checksum:i3EC99D96E6C0114C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73 – 78TPPGGG → HPTRC in AAB23374 (PubMed:2152343).Curated6
Sequence conflicti263A → S in AAB23374 (PubMed:2152343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16938 Genomic DNA Translation: CAA34812.1
X16939 mRNA Translation: CAA34813.1
S44869 mRNA Translation: AAB23374.1
PIRiS08627
RefSeqiNP_001311556.1, NM_001324627.1
UniGeneiNta.9572

Genome annotation databases

GeneIDi107759005
KEGGinta:107759005

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16938 Genomic DNA Translation: CAA34812.1
X16939 mRNA Translation: CAA34813.1
S44869 mRNA Translation: AAB23374.1
PIRiS08627
RefSeqiNP_001311556.1, NM_001324627.1
UniGeneiNta.9572

3D structure databases

ProteinModelPortaliP08252
SMRiP08252
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Proteomic databases

PRIDEiP08252
ProMEXiP08252

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107759005
KEGGinta:107759005

Phylogenomic databases

KOiK20547

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHI1_TOBAC
AccessioniPrimary (citable) accession number: P08252
Secondary accession number(s): Q41180
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1990
Last modified: October 10, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again