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Entry version 180 (07 Apr 2021)
Sequence version 4 (01 Nov 1995)
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Protein

Nitrite reductase (NADH) large subunit

Gene

nirB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi641Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi647Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi681Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi685Iron (siroheme axial ligand)By similarity1
Metal bindingi685Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi44 – 79FADSequence analysisAdd BLAST36
Nucleotide bindingi193 – 225NAD or NADPSequence analysisAdd BLAST33

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
Ligand4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:NIRB-MONOMER
MetaCyc:NIRB-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00653

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nitrite reductase (NADH) large subunit (EC:1.7.1.15)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nirB
Ordered Locus Names:b3365, JW3328
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001999621 – 847Nitrite reductase (NADH) large subunitAdd BLAST847

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P08201

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P08201

PRoteomics IDEntifications database

More...
PRIDEi
P08201

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer which associates with NirD.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261087, 21 interactors
852179, 1 interactor

Protein interaction database and analysis system

More...
IntActi
P08201, 1 interactor

STRING: functional protein association networks

More...
STRINGi
511145.b3365

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1251, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_003291_0_0_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P08201

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P08201

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.1100, 1 hit
3.50.50.60, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR007419, BFD-like_2Fe2S-bd_dom
IPR041854, BFD-like_2Fe2S-bd_dom_sf
IPR036188, FAD/NAD-bd_sf
IPR023753, FAD/NAD-binding_dom
IPR005117, NiRdtase/SiRdtase_haem-b_fer
IPR036136, Nit/Sulf_reduc_fer-like_dom_sf
IPR012744, Nitri_red_NirB
IPR017121, Nitrite_Rdtase_lsu
IPR006067, NO2/SO3_Rdtase_4Fe4S_dom
IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS
IPR041575, Rubredoxin_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04324, Fer2_BFD, 1 hit
PF01077, NIR_SIR, 1 hit
PF03460, NIR_SIR_ferr, 1 hit
PF07992, Pyr_redox_2, 1 hit
PF18267, Rubredoxin_C, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF037149, NirB, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00397, SIROHAEM

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51905, SSF51905, 2 hits
SSF55124, SSF55124, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02374, nitri_red_nirB, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00365, NIR_SIR, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P08201-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS
60 70 80 90 100
SYFSHHTAEE LSLVREGFYE KHGIKVLVGE RAITINRQEK VIHSSAGRTV
110 120 130 140 150
FYDKLIMATG SYPWIPPIKG SDTQDCFVYR TIEDLNAIES CARRSKRGAV
160 170 180 190 200
VGGGLLGLEA AGALKNLGIE THVIEFAPML MAEQLDQMGG EQLRRKIESM
210 220 230 240 250
GVRVHTSKNT LEIVQEGVEA RKTMRFADGS ELEVDFIVFS TGIRPRDKLA
260 270 280 290 300
TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG ECASWNNRVF GLVAPGYKMA
310 320 330 340 350
QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD
360 370 380 390 400
ESKEIYKRLI VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL
410 420 430 440 450
ILPAHSGSGK PSIGVDKLPD SAQICSCFDV TKGDLIAAIN KGCHTVAALK
460 470 480 490 500
AETKAGTGCG GCIPLVTQVL NAELAKQGIE VNNNLCEHFA YSRQELFHLI
510 520 530 540 550
RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI LKPEHTPLQD
560 570 580 590 600
SNDNFLANIQ KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS
610 620 630 640 650
QRLAMFGAQK DDLPEIWRQL IEAGFETGHA YAKALRMAKT CVGSTWCRYG
660 670 680 690 700
VGDSVGLGVE LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK
710 720 730 740 750
GWNLYVCGNG GMKPRHADLL AADIDRETLI KYLDRFMMFY IRTADKLTRT
760 770 780 790 800
APWLENLEGG IDYLKAVIID DKLGLNAHLE EEMARLREAV LCEWTETVNT
810 820 830 840
PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV TLVEDNA
Length:847
Mass (Da):93,121
Last modified:November 1, 1995 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5265AD93FD390EB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti442G → A (PubMed:2543955).Curated1
Sequence conflicti442G → A (PubMed:2200672).Curated1
Sequence conflicti835 – 847YERIP…VEDNA → MNVSQ (PubMed:2543955).CuratedAdd BLAST13
Sequence conflicti835 – 847YERIP…VEDNA → MNVSQ (PubMed:2200672).CuratedAdd BLAST13

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X14202 Genomic DNA Translation: CAA32416.1
U18997 Genomic DNA Translation: AAA58162.1
U00096 Genomic DNA Translation: AAC76390.1
AP009048 Genomic DNA Translation: BAE77925.1

Protein sequence database of the Protein Information Resource

More...
PIRi
H65130

NCBI Reference Sequences

More...
RefSeqi
NP_417824.1, NC_000913.3
WP_000049208.1, NZ_SSZK01000008.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76390; AAC76390; b3365
BAE77925; BAE77925; BAE77925

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
57729370
947868

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3328
eco:b3365

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3364

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA Translation: CAA32416.1
U18997 Genomic DNA Translation: AAA58162.1
U00096 Genomic DNA Translation: AAC76390.1
AP009048 Genomic DNA Translation: BAE77925.1
PIRiH65130
RefSeqiNP_417824.1, NC_000913.3
WP_000049208.1, NZ_SSZK01000008.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4261087, 21 interactors
852179, 1 interactor
IntActiP08201, 1 interactor
STRINGi511145.b3365

Proteomic databases

jPOSTiP08201
PaxDbiP08201
PRIDEiP08201

Genome annotation databases

EnsemblBacteriaiAAC76390; AAC76390; b3365
BAE77925; BAE77925; BAE77925
GeneIDi57729370
947868
KEGGiecj:JW3328
eco:b3365
PATRICifig|1411691.4.peg.3364

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0647

Phylogenomic databases

eggNOGiCOG1251, Bacteria
HOGENOMiCLU_003291_0_0_6
InParanoidiP08201
PhylomeDBiP08201

Enzyme and pathway databases

UniPathwayiUPA00653
BioCyciEcoCyc:NIRB-MONOMER
MetaCyc:NIRB-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P08201

Family and domain databases

Gene3Di1.10.10.1100, 1 hit
3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR007419, BFD-like_2Fe2S-bd_dom
IPR041854, BFD-like_2Fe2S-bd_dom_sf
IPR036188, FAD/NAD-bd_sf
IPR023753, FAD/NAD-binding_dom
IPR005117, NiRdtase/SiRdtase_haem-b_fer
IPR036136, Nit/Sulf_reduc_fer-like_dom_sf
IPR012744, Nitri_red_NirB
IPR017121, Nitrite_Rdtase_lsu
IPR006067, NO2/SO3_Rdtase_4Fe4S_dom
IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS
IPR041575, Rubredoxin_C
PfamiView protein in Pfam
PF04324, Fer2_BFD, 1 hit
PF01077, NIR_SIR, 1 hit
PF03460, NIR_SIR_ferr, 1 hit
PF07992, Pyr_redox_2, 1 hit
PF18267, Rubredoxin_C, 1 hit
PIRSFiPIRSF037149, NirB, 1 hit
PRINTSiPR00397, SIROHAEM
SUPFAMiSSF51905, SSF51905, 2 hits
SSF55124, SSF55124, 1 hit
TIGRFAMsiTIGR02374, nitri_red_nirB, 1 hit
PROSITEiView protein in PROSITE
PS00365, NIR_SIR, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIRB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P08201
Secondary accession number(s): Q2M731
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1995
Last modified: April 7, 2021
This is version 180 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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