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Protein

Peptidase 1

Gene

DERP1

Organism
Dermatophagoides pteronyssinus (European house dust mite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei132By similarity1
Active sitei268By similarity1
Active sitei288By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.65 1873

Protein family/group databases

MEROPSiC01.073

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase 1 (EC:3.4.22.65)
Alternative name(s):
Allergen Der p I
Major mite fecal allergen Der p 1
Allergen: Der p 1
Gene namesi
Name:DERP1
OrganismiDermatophagoides pteronyssinus (European house dust mite)
Taxonomic identifieri6956 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis. Binds to IgE in 80% of patients with house dust allergy.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi132C → A: Loss of activity. 1
Mutagenesisi150N → E: Loss of N-glycosylation. 1

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1232 Der p 1.0111
310 Der p 1

Chemistry databases

ChEMBLiCHEMBL3351204

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
PropeptideiPRO_000002637619 – 98Activation peptide3 PublicationsAdd BLAST80
ChainiPRO_000002637799 – 320Peptidase 1Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi102 ↔ 215
Disulfide bondi129 ↔ 169
Glycosylationi150N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi163 ↔ 201

Post-translational modificationi

N-glycosylated. N-glycanase treatment does not completely remove carbohydrates, suggesting that the protein contains additional glycosylation sites.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

PTM databases

iPTMnetiP08176

Interactioni

Chemistry databases

BindingDBiP08176

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08176
SMRiP08176
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08176

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08176-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIVLAIASL LALSAVYARP SSIKTFEEYK KAFNKSYATF EDEEAARKNF
60 70 80 90 100
LESVKYVQSN GGAINHLSDL SLDEFKNRFL MSAEAFEHLK TQFDLNAETN
110 120 130 140 150
ACSINGNAPA EIDLRQMRTV TPIRMQGGCG SCWAFSGVAA TESAYLAYRN
160 170 180 190 200
QSLDLAEQEL VDCASQHGCH GDTIPRGIEY IQHNGVVQES YYRYVAREQS
210 220 230 240 250
CRRPNAQRFG ISNYCQIYPP NVNKIREALA QTHSAIAVII GIKDLDAFRH
260 270 280 290 300
YDGRTIIQRD NGYQPNYHAV NIVGYSNAQG VDYWIVRNSW DTNWGDNGYG
310 320
YFAANIDLMM IEEYPYVVIL
Length:320
Mass (Da):36,104
Last modified:February 1, 1995 - v2
Checksum:iA0B1F4DD09791DFE
GO

Sequence cautioni

The sequence AAA28296 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti148Y → H. 1
Natural varianti179E → K. 1
Natural varianti222V → A1 Publication1
Natural varianti234S → T. 1
Natural varianti313E → Q. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11695 mRNA Translation: AAB60215.1
M24794 mRNA Translation: AAA28296.1 Different initiation.
X65197 Genomic DNA Translation: CAA46317.1
PIRiJQ0337

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11695 mRNA Translation: AAB60215.1
M24794 mRNA Translation: AAA28296.1 Different initiation.
X65197 Genomic DNA Translation: CAA46317.1
PIRiJQ0337

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XKGX-ray1.61A19-320[»]
2AS8X-ray1.95A/B99-320[»]
3RVWX-ray1.95A99-320[»]
3RVXX-ray2.50A99-320[»]
4PP1X-ray3.00A/B99-320[»]
4PP2X-ray2.74E/F99-320[»]
5VCNX-ray3.00A/B99-320[»]
5VCOX-ray2.74E/F99-320[»]
5VPHX-ray2.50A99-320[»]
ProteinModelPortaliP08176
SMRiP08176
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP08176
ChEMBLiCHEMBL3351204

Protein family/group databases

Allergomei1232 Der p 1.0111
310 Der p 1
MEROPSiC01.073

PTM databases

iPTMnetiP08176

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.22.65 1873

Miscellaneous databases

EvolutionaryTraceiP08176

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEPT1_DERPT
AccessioniPrimary (citable) accession number: P08176
Secondary accession number(s): Q24616
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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