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UniProtKB - P08176 (PEPT1_DERPT)
Protein
Peptidase 1
Gene
DERP1
Organism
Dermatophagoides pteronyssinus (European house dust mite)
Status
Functioni
Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.
Catalytic activityi
- Broad endopeptidase specificity. EC:3.4.22.65
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 132 | By similarity | 1 | |
Active sitei | 268 | By similarity | 1 | |
Active sitei | 288 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.65, 1873 |
Protein family/group databases
MEROPSi | C01.073 |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidase 1 (EC:3.4.22.65)Alternative name(s): Allergen Der p I Major mite fecal allergen Der p 1 Allergen: Der p 1 |
Gene namesi | Name:DERP1 |
Organismi | Dermatophagoides pteronyssinus (European house dust mite) |
Taxonomic identifieri | 6956 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Acari › Acariformes › Sarcoptiformes › Astigmata › Psoroptidia › Analgoidea › Pyroglyphidae › Dermatophagoidinae › Dermatophagoides |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis. Binds to IgE in 80% of patients with house dust allergy.
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 132 | C → A: Loss of activity. | 1 | |
Mutagenesisi | 150 | N → E: Loss of N-glycosylation. | 1 |
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 1232, Der p 1.0111 310, Der p 1 |
Chemistry databases
ChEMBLi | CHEMBL3351204 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 1 PublicationAdd BLAST | 18 | |
PropeptideiPRO_0000026376 | 19 – 98 | Activation peptide3 PublicationsAdd BLAST | 80 | |
ChainiPRO_0000026377 | 99 – 320 | Peptidase 1Add BLAST | 222 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 102 ↔ 215 | |||
Disulfide bondi | 129 ↔ 169 | |||
Glycosylationi | 150 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 163 ↔ 201 |
Post-translational modificationi
N-glycosylated. N-glycanase treatment does not completely remove carbohydrates, suggesting that the protein contains additional glycosylation sites.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenPTM databases
iPTMneti | P08176 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08176 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08176 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P08176-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIVLAIASL LALSAVYARP SSIKTFEEYK KAFNKSYATF EDEEAARKNF
60 70 80 90 100
LESVKYVQSN GGAINHLSDL SLDEFKNRFL MSAEAFEHLK TQFDLNAETN
110 120 130 140 150
ACSINGNAPA EIDLRQMRTV TPIRMQGGCG SCWAFSGVAA TESAYLAYRN
160 170 180 190 200
QSLDLAEQEL VDCASQHGCH GDTIPRGIEY IQHNGVVQES YYRYVAREQS
210 220 230 240 250
CRRPNAQRFG ISNYCQIYPP NVNKIREALA QTHSAIAVII GIKDLDAFRH
260 270 280 290 300
YDGRTIIQRD NGYQPNYHAV NIVGYSNAQG VDYWIVRNSW DTNWGDNGYG
310 320
YFAANIDLMM IEEYPYVVIL
Sequence cautioni
The sequence AAA28296 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 148 | Y → H. | 1 | |
Natural varianti | 179 | E → K. | 1 | |
Natural varianti | 222 | V → A1 Publication | 1 | |
Natural varianti | 234 | S → T. | 1 | |
Natural varianti | 313 | E → Q. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11695 mRNA Translation: AAB60215.1 M24794 mRNA Translation: AAA28296.1 Different initiation. X65197 Genomic DNA Translation: CAA46317.1 |
PIRi | JQ0337 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11695 mRNA Translation: AAB60215.1 M24794 mRNA Translation: AAA28296.1 Different initiation. X65197 Genomic DNA Translation: CAA46317.1 |
PIRi | JQ0337 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XKG | X-ray | 1.61 | A | 19-320 | [»] | |
2AS8 | X-ray | 1.95 | A/B | 99-320 | [»] | |
3RVW | X-ray | 1.95 | A | 99-320 | [»] | |
3RVX | X-ray | 2.50 | A | 99-320 | [»] | |
4PP1 | X-ray | 3.00 | A/B | 99-320 | [»] | |
4PP2 | X-ray | 2.74 | E/F | 99-320 | [»] | |
5VCN | X-ray | 3.00 | A/B | 99-320 | [»] | |
5VCO | X-ray | 2.74 | E/F | 99-320 | [»] | |
5VPH | X-ray | 2.50 | A | 99-320 | [»] | |
SMRi | P08176 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | P08176 |
ChEMBLi | CHEMBL3351204 |
Protein family/group databases
Allergomei | 1232, Der p 1.0111 310, Der p 1 |
MEROPSi | C01.073 |
PTM databases
iPTMneti | P08176 |
Protocols and materials databases
ABCDi | P08176, 6 sequenced antibodies |
Enzyme and pathway databases
BRENDAi | 3.4.22.65, 1873 |
Miscellaneous databases
EvolutionaryTracei | P08176 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PEPT1_DERPT | |
Accessioni | P08176Primary (citable) accession number: P08176 Secondary accession number(s): Q24616 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | February 1, 1995 | |
Last modified: | June 2, 2021 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Allergens
Nomenclature of allergens and list of entries - Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families