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Protein

Complement decay-accelerating factor

Gene

CD55

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).1 Publication1 Publication
(Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.1 Publication
(Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).1 Publication
(Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.1 Publication1 Publication

GO - Molecular functioni

  • lipid binding Source: UniProtKB
  • virus receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionBlood group antigen, Host cell receptor for virus entry, Receptor
Biological processComplement pathway, Host-virus interaction, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-373080 Class B/2 (Secretin family receptors)
R-HSA-6798695 Neutrophil degranulation
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-977606 Regulation of Complement cascade
SIGNORiP08174

Names & Taxonomyi

Protein namesi
Recommended name:
Complement decay-accelerating factor
Alternative name(s):
CD_antigen: CD55
Gene namesi
Name:CD55
Synonyms:CR, DAF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000196352.14
HGNCiHGNC:2665 CD55
MIMi125240 gene
neXtProtiNX_P08174

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Complement hyperactivation, angiopathic thrombosis, and protein-losing enteropathy (CHAPLE)2 Publications
The disease is caused by mutations affecting the gene represented in this entry. CHAPLE is caused by biallelic mutations in the CD55 gene.
Disease descriptionAn autosomal recessive disease characterized by abdominal pain and diarrhea, primary intestinal lymphangiectasia, edema due to hypoproteinemia, malabsorption, and less frequently, bowel inflammation, recurrent infections, and angiopathic thromboembolic disease. Patients' T lymphocytes show increased complement activation causing surface deposition of complement and the generation of soluble C5a.
See also OMIM:226300
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_079373267C → S in CHAPLE; increased complement activation. 1 PublicationCorresponds to variant dbSNP:rs1135402917Ensembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi1604
MalaCardsiCD55
MIMi226300 phenotype
613793 phenotype
OpenTargetsiENSG00000196352
PharmGKBiPA27137

Chemistry databases

DrugBankiDB00446 Chloramphenicol

Polymorphism and mutation databases

BioMutaiCD55
DMDMi60416353

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 342 PublicationsAdd BLAST34
ChainiPRO_000000600035 – 353Complement decay-accelerating factorAdd BLAST319
PropeptideiPRO_0000006001354 – 381Removed in mature formAdd BLAST28

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 81PROSITE-ProRule annotation1 Publication
Disulfide bondi65 ↔ 94PROSITE-ProRule annotation1 Publication
Glycosylationi95N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi98 ↔ 145PROSITE-ProRule annotation1 Publication
Disulfide bondi129 ↔ 158PROSITE-ProRule annotation1 Publication
Disulfide bondi163 ↔ 204PROSITE-ProRule annotation1 Publication
Disulfide bondi190 ↔ 220PROSITE-ProRule annotation1 Publication
Disulfide bondi225 ↔ 267PROSITE-ProRule annotation1 Publication
Disulfide bondi253 ↔ 283PROSITE-ProRule annotation1 Publication
Lipidationi353GPI-anchor amidated serine1 Publication1

Post-translational modificationi

The Ser/Thr-rich domain is heavily O-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP08174
PaxDbiP08174
PeptideAtlasiP08174
PRIDEiP08174
ProteomicsDBi52078
52079 [P08174-2]

PTM databases

iPTMnetiP08174
PhosphoSitePlusiP08174
SwissPalmiP08174

Expressioni

Tissue specificityi

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

Gene expression databases

BgeeiENSG00000196352
CleanExiHS_CD55
ExpressionAtlasiP08174 baseline and differential
GenevisibleiP08174 HS

Organism-specific databases

HPAiCAB010454
HPA002190
HPA024386

Interactioni

Subunit structurei

Monomer (major form) and non-disulfide-linked, covalent homodimer (minor form).1 Publication1 Publication
(Microbial infection) Interacts with coxsackievirus A21, coxsackieviruses B1, B3 and B5 capsid proteins.1 Publication
(Microbial infection) Interacts with human enterovirus 70 and D68 capsid proteins (Probable).1 Publication
(Microbial infection) Interacts with human echoviruses 6, 7, 11, 12, 20 and 21 capsid proteins.1 Publication

Protein-protein interaction databases

BioGridi107974, 27 interactors
IntActiP08174, 7 interactors
STRINGi9606.ENSP00000316333

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi45 – 47Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi69 – 71Combined sources3
Beta strandi78 – 82Combined sources5
Turni83 – 85Combined sources3
Beta strandi94 – 98Combined sources5
Beta strandi105 – 109Combined sources5
Helixi113 – 115Combined sources3
Beta strandi124 – 129Combined sources6
Beta strandi133 – 135Combined sources3
Beta strandi136 – 138Combined sources3
Beta strandi142 – 145Combined sources4
Beta strandi149 – 151Combined sources3
Beta strandi157 – 160Combined sources4
Beta strandi172 – 175Combined sources4
Turni177 – 180Combined sources4
Beta strandi185 – 190Combined sources6
Beta strandi194 – 198Combined sources5
Beta strandi200 – 207Combined sources8
Beta strandi210 – 215Combined sources6
Beta strandi219 – 222Combined sources4
Beta strandi234 – 236Combined sources3
Beta strandi241 – 244Combined sources4
Beta strandi248 – 253Combined sources6
Beta strandi258 – 261Combined sources4
Beta strandi263 – 270Combined sources8
Beta strandi273 – 278Combined sources6
Beta strandi282 – 284Combined sources3

3D structure databases

ProteinModelPortaliP08174
SMRiP08174
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08174

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 96Sushi 1PROSITE-ProRule annotationAdd BLAST62
Domaini96 – 160Sushi 2PROSITE-ProRule annotationAdd BLAST65
Domaini161 – 222Sushi 3PROSITE-ProRule annotationAdd BLAST62
Domaini223 – 285Sushi 4PROSITE-ProRule annotationAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi287 – 356Ser/Thr-richAdd BLAST70

Domaini

The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3 (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IEGQ Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00910000143999
HOGENOMiHOG000237360
HOVERGENiHBG001406
InParanoidiP08174
KOiK04006
PhylomeDBiP08174
TreeFamiTF334137

Family and domain databases

CDDicd00033 CCP, 4 hits
InterProiView protein in InterPro
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
PfamiView protein in Pfam
PF00084 Sushi, 4 hits
SMARTiView protein in SMART
SM00032 CCP, 4 hits
SUPFAMiSSF57535 SSF57535, 4 hits
PROSITEiView protein in PROSITE
PS50923 SUSHI, 4 hits

Sequences (7)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P08174-1) [UniParc]FASTAAdd to basket
Also known as: DAF-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVARPSVPA ALPLLGELPR LLLLVLLCLP AVWGDCGLPP DVPNAQPALE
60 70 80 90 100
GRTSFPEDTV ITYKCEESFV KIPGEKDSVI CLKGSQWSDI EEFCNRSCEV
110 120 130 140 150
PTRLNSASLK QPYITQNYFP VGTVVEYECR PGYRREPSLS PKLTCLQNLK
160 170 180 190 200
WSTAVEFCKK KSCPNPGEIR NGQIDVPGGI LFGATISFSC NTGYKLFGST
210 220 230 240 250
SSFCLISGSS VQWSDPLPEC REIYCPAPPQ IDNGIIQGER DHYGYRQSVT
260 270 280 290 300
YACNKGFTMI GEHSIYCTVN NDEGEWSGPP PECRGKSLTS KVPPTVQKPT
310 320 330 340 350
TVNVPTTEVS PTSQKTTTKT TTPNAQATRS TPVSRTTKHF HETTPNKGSG
360 370 380
TTSGTTRLLS GHTCFTLTGL LGTLVTMGLL T
Length:381
Mass (Da):41,400
Last modified:March 1, 2005 - v4
Checksum:iC1CBE5300F60C176
GO
Isoform 1 (identifier: P08174-2) [UniParc]FASTAAdd to basket
Also known as: DAF-1

The sequence of this isoform differs from the canonical sequence as follows:
     362-381: HTCFTLTGLLGTLVTMGLLT → SRPVTQAGMR...TQVYRLFLVS

Show »
Length:440
Mass (Da):48,717
Checksum:iCF6154031FAC5D58
GO
Isoform 3 (identifier: P08174-3) [UniParc]FASTAAdd to basket
Also known as: VDAF3

The sequence of this isoform differs from the canonical sequence as follows:
     361-381: GHTCFTLTGLLGTLVTMGLLT → ALQVRPFEVSGSSHISSKKMMCIL

Show »
Length:384
Mass (Da):41,900
Checksum:i192116A331DBD533
GO
Isoform 4 (identifier: P08174-4) [UniParc]FASTAAdd to basket
Also known as: VDAF2

The sequence of this isoform differs from the canonical sequence as follows:
     361-381: GHTCFTLTGLLGTLVTMGLLT → VLFM

Show »
Length:364
Mass (Da):39,759
Checksum:iBFDE9FD6B7C10A07
GO
Isoform 5 (identifier: P08174-5) [UniParc]FASTAAdd to basket
Also known as: VDAF1

The sequence of this isoform differs from the canonical sequence as follows:
     361-381: GHTCFTLTGLLGTLVTMGLLT → ETVFHRVIQD...TQVYRLFLVS

Show »
Length:439
Mass (Da):48,513
Checksum:i256086EFD8CDBDBA
GO
Isoform 6 (identifier: P08174-6) [UniParc]FASTAAdd to basket
Also known as: VDAF4

The sequence of this isoform differs from the canonical sequence as follows:
     327-327: A → GTETPSVLQK...AFTQSPSAAP

Note: Includes partial sequence of the intron 7.
Show »
Length:525
Mass (Da):56,218
Checksum:i1AAFC1E5FD7DCE4C
GO
Isoform 7 (identifier: P08174-7) [UniParc]FASTAAdd to basket
Also known as: VDAF5

The sequence of this isoform differs from the canonical sequence as follows:
     326-326: Q → QGTETPSVLQ...TQRFPSAHIT

Note: Includes full sequence of the intron 7.
Show »
Length:551
Mass (Da):59,038
Checksum:i505AEC29D919AF48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → T in AAA52170 (PubMed:1711208).Curated1
Sequence conflicti80I → T in AAA52167 (PubMed:2436222).Curated1
Sequence conflicti85S → M in AAA52167 (PubMed:2436222).Curated1
Sequence conflicti187S → T in AAB48622 (Ref. 10) Curated1
Sequence conflicti297Q → H in AAB48622 (Ref. 10) Curated1

Polymorphismi

Responsible for the Cromer blood group system (CROM) [MIMi:613793]. It consists of at least 8 high-incidence (Cr(a), Tc(a), Dr(a), Es(a), WES(b), UMC, IFC and GUTI) and three low-incidence (Tc(b), Tc(c) and WES(a)) antigens that reside on DAF. In the Cromer phenotypes Dr(a-) and Inab there is reduced or absent expression of DAF, respectively. In the case of the Dr(a-) phenotype, a single nucleotide substitution within exon 5 accounts for two changes: a simple amino acid substitution, Leu-199 that is the basis of the antigenic variation, and an alternative splicing event that underlies the decreased expression of DAF in this phenotype. The Inab phenotype is a very rare one in which the red blood cells lack all Cromer system antigens. The red blood cells of individuals with Inab phenotype have a deficiency of DAF, but these individuals are not known to have any associated hematologic or other abnormalities (PubMed:12675719).1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00199752R → L in Tc(b) antigen. 1 PublicationCorresponds to variant dbSNP:rs28371588Ensembl.1
Natural variantiVAR_00199852R → P in Tc(c) antigen. Corresponds to variant dbSNP:rs28371588Ensembl.1
Natural variantiVAR_00199982L → R in WES(a) antigen. Corresponds to variant dbSNP:rs147474393Ensembl.1
Natural variantiVAR_002000199S → L in Dr(a-) antigen. 1 PublicationCorresponds to variant dbSNP:rs1135402914Ensembl.1
Natural variantiVAR_002001227A → P in Cr(a-) antigen. Corresponds to variant dbSNP:rs60822373Ensembl.1
Natural variantiVAR_015884240R → H in GUTI(-) antigen. 1 PublicationCorresponds to variant dbSNP:rs199705465Ensembl.1
Natural variantiVAR_079373267C → S in CHAPLE; increased complement activation. 1 PublicationCorresponds to variant dbSNP:rs1135402917Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047634326Q → QGTETPSVLQKHTTENVSAT RTPPTPQKPTTVNVPATIVT PTPQKPTTINVPATGVSSTP QRHTIVNVSATGTLPTLQKP TRANDSATKSPAAAQTSFIS KTLSTKTPSAAQNPMMTNAS ATQATLTAQKFTTAKVAFTQ SPSAARKSTNVHSPVTNGLK STQRFPSAHIT in isoform 7. 1 Publication1
Alternative sequenceiVSP_047635327A → GTETPSVLQKHTTENVSATR TPPTPQKPTTVNVPATIVTP TPQKPTTINVPATGVSSTPQ RHTIVNVSATGTLPTLQKPT RANDSATKSPAAAQTSFISK TLSTKTPSAAQNPMMTNASA TQATLTAQKFTTAKVAFTQS PSAAP in isoform 6. 1 Publication1
Alternative sequenceiVSP_047636361 – 381GHTCF…MGLLT → ALQVRPFEVSGSSHISSKKM MCIL in isoform 3. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_047637361 – 381GHTCF…MGLLT → VLFM in isoform 4. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_047638361 – 381GHTCF…MGLLT → ETVFHRVIQDGLDLLASRSA CLGLPKCWDYRREPPHLARA HVFHVDRFAWDASNHGLADL AKEELRRKYTQVYRLFLVS in isoform 5. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_001200362 – 381HTCFT…MGLLT → SRPVTQAGMRWCDRSSLQSR TPGFKRSFHFSLPSSWYYRA HVFHVDRFAWDASNHGLADL AKEELRRKYTQVYRLFLVS in isoform 1. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31516 mRNA Translation: AAA52169.1
M30142 mRNA Translation: AAA52168.1
AB240566 mRNA Translation: BAE97422.1
AB240567 mRNA Translation: BAE97423.1
AB240568 mRNA Translation: BAE97424.1
AB240569 mRNA Translation: BAE97425.1
AB240570 mRNA Translation: BAE97426.1
BT007159 mRNA Translation: AAP35823.1
AY851161 Genomic DNA Translation: AAW29942.1
AL391597, AL596218 Genomic DNA Translation: CAH72946.1
AL596218, AL391597 Genomic DNA Translation: CAI16463.1
CH471100 Genomic DNA Translation: EAW93485.1
CH471100 Genomic DNA Translation: EAW93487.1
CH471100 Genomic DNA Translation: EAW93488.1
CH471100 Genomic DNA Translation: EAW93491.1
BC001288 mRNA Translation: AAH01288.1
M64653, M64356 Genomic DNA Translation: AAA52170.1
M15799 mRNA Translation: AAA52167.1
U88576 mRNA Translation: AAB48622.1
S72858 Genomic DNA Translation: AAC60633.1
CCDSiCCDS31006.1 [P08174-1]
CCDS44307.1 [P08174-2]
PIRiA26359
B26359
RefSeqiNP_000565.1, NM_000574.4 [P08174-1]
NP_001108224.1, NM_001114752.2 [P08174-2]
NP_001287832.1, NM_001300903.1 [P08174-5]
NP_001287833.1, NM_001300904.1 [P08174-3]
UniGeneiHs.126517
Hs.609950

Genome annotation databases

EnsembliENST00000314754; ENSP00000316333; ENSG00000196352 [P08174-2]
ENST00000367064; ENSP00000356031; ENSG00000196352 [P08174-1]
ENST00000644836; ENSP00000495518; ENSG00000196352 [P08174-3]
ENST00000645323; ENSP00000496251; ENSG00000196352 [P08174-5]
GeneIDi1604
KEGGihsa:1604
UCSCiuc001hfq.5 human [P08174-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDAF_HUMAN
AccessioniPrimary (citable) accession number: P08174
Secondary accession number(s): B1AP14
, D3DT83, D3DT84, E7ER69, P09679, P78361, Q14UF2, Q14UF3, Q14UF4, Q14UF5, Q14UF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: March 1, 2005
Last modified: July 18, 2018
This is version 209 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  8. SIMILARITY comments
    Index of protein domains and families

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