Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muscarinic acetylcholine receptor M4

Gene

CHRM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-390648 Muscarinic acetylcholine receptors
R-HSA-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:CHRM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000180720.7
HGNCiHGNC:1953 CHRM4
MIMi118495 gene
neXtProtiNX_P08173

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31ExtracellularBy similarityAdd BLAST31
Transmembranei32 – 54Helical; Name=1By similarityAdd BLAST23
Topological domaini55 – 68CytoplasmicBy similarityAdd BLAST14
Transmembranei69 – 89Helical; Name=2By similarityAdd BLAST21
Topological domaini90 – 106ExtracellularBy similarityAdd BLAST17
Transmembranei107 – 128Helical; Name=3By similarityAdd BLAST22
Topological domaini129 – 148CytoplasmicBy similarityAdd BLAST20
Transmembranei149 – 171Helical; Name=4By similarityAdd BLAST23
Topological domaini172 – 193ExtracellularBy similarityAdd BLAST22
Transmembranei194 – 216Helical; Name=5By similarityAdd BLAST23
Topological domaini217 – 401CytoplasmicBy similarityAdd BLAST185
Transmembranei402 – 422Helical; Name=6By similarityAdd BLAST21
Topological domaini423 – 436ExtracellularBy similarityAdd BLAST14
Transmembranei437 – 456Helical; Name=7By similarityAdd BLAST20
Topological domaini457 – 479CytoplasmicBy similarityAdd BLAST23

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1132
OpenTargetsiENSG00000180720
PharmGKBiPA26485

Chemistry databases

ChEMBLiCHEMBL1821
DrugBankiDB08897 Aclidinium
DB05752 ALKS 27
DB00321 Amitriptyline
DB00543 Amoxapine
DB01238 Aripiprazole
DB00572 Atropine
DB00767 Benzquinamide
DB00835 Brompheniramine
DB01239 Chlorprothixene
DB00568 Cinnarizine
DB00363 Clozapine
DB00785 Cryptenamine
DB00496 Darifenacin
DB01151 Desipramine
DB01142 Doxepin
DB06702 Fesoterodine
DB00725 Homatropine Methylbromide
DB00424 Hyoscyamine
DB00458 Imipramine
DB01625 Isopropamide
DB01221 Ketamine
DB00408 Loxapine
DB00934 Maprotiline
DB01403 Methotrimeprazine
DB00340 Metixene
DB00622 Nicardipine
DB00540 Nortriptyline
DB00334 Olanzapine
DB00715 Paroxetine
DB00454 Pethidine
DB00387 Procyclidine
DB00420 Promazine
DB01069 Promethazine
DB00777 Propiomazine
DB01224 Quetiapine
DB00747 Scopolamine
DB01591 Solifenacin
DB00342 Terfenadine
DB01036 Tolterodine
DB00376 Trihexyphenidyl
DB00726 Trimipramine
DB00809 Tropicamide
DB09076 Umeclidinium
DB00246 Ziprasidone
GuidetoPHARMACOLOGYi16

Polymorphism and mutation databases

BioMutaiCHRM4
DMDMi23503039

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690371 – 479Muscarinic acetylcholine receptor M4Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi13N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi105 ↔ 185PROSITE-ProRule annotation
Modified residuei459PhosphothreonineSequence analysis1
Modified residuei463PhosphothreonineSequence analysis1
Modified residuei477PhosphothreonineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08173
PeptideAtlasiP08173
PRIDEiP08173
ProteomicsDBi52077

PTM databases

iPTMnetiP08173
PhosphoSitePlusiP08173

Expressioni

Gene expression databases

BgeeiENSG00000180720
CleanExiHS_CHRM4
GenevisibleiP08173 HS

Organism-specific databases

HPAiCAB022444
HPA072083

Interactioni

Protein-protein interaction databases

BioGridi107554, 74 interactors
DIPiDIP-61455N
IntActiP08173, 3 interactors
STRINGi9606.ENSP00000409378

Chemistry databases

BindingDBiP08173

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 59Combined sources34
Helixi61 – 63Combined sources3
Helixi66 – 83Combined sources18
Helixi85 – 95Combined sources11
Helixi101 – 135Combined sources35
Turni137 – 140Combined sources4
Helixi141 – 143Combined sources3
Helixi146 – 169Combined sources24
Helixi171 – 175Combined sources5
Helixi188 – 190Combined sources3
Helixi193 – 203Combined sources11
Helixi205 – 220Combined sources16
Beta strandi223 – 225Combined sources3
Helixi397 – 422Combined sources26
Turni423 – 425Combined sources3
Helixi432 – 456Combined sources25
Helixi458 – 468Combined sources11

3D structure databases

ProteinModelPortaliP08173
SMRiP08173
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP08173
KOiK04132
OMAiTSTHNRY
OrthoDBiEOG091G06VI
PhylomeDBiP08173
TreeFamiTF320495

Family and domain databases

CDDicd15298 7tmA_mAChR_M4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001432 Musac_Ach_M4_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF6 PTHR43914:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00541 MUSCRINICM4R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P08173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN
60 70 80 90 100
ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL
110 120 130 140 150
GAVVCDLWLA LDYVVSNASV MNLLIISFDR YFCVTKPLTY PARRTTKMAG
160 170 180 190 200
LMIAAAWVLS FVLWAPAILF WQFVVGKRTV PDNQCFIQFL SNPAVTFGTA
210 220 230 240 250
IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK TLAFLKSPLM
260 270 280 290 300
KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS
310 320 330 340 350
ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG
360 370 380 390 400
NECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PDTVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):53,049
Last modified:September 19, 2002 - v2
Checksum:i9C1B05B359A64289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16405 Genomic DNA Translation: AAA51571.2
X15265 Genomic DNA Translation: CAA33336.1
AF498918 mRNA Translation: AAM18941.1
CH471064 Genomic DNA Translation: EAW68004.1
BC095546 mRNA Translation: AAH95546.1
BC119775 mRNA Translation: AAI19776.1
BC119817 mRNA Translation: AAI19818.1
BC137535 mRNA Translation: AAI37536.1
CCDSiCCDS44581.1
PIRiS10127
RefSeqiNP_000732.2, NM_000741.3
UniGeneiHs.248100

Genome annotation databases

EnsembliENST00000433765; ENSP00000409378; ENSG00000180720
GeneIDi1132
KEGGihsa:1132
UCSCiuc001nct.2 human

Similar proteinsi

Entry informationi

Entry nameiACM4_HUMAN
AccessioniPrimary (citable) accession number: P08173
Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 19, 2002
Last modified: June 20, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health