UniProtKB - P08166 (KAD2_BOVIN)
Adenylate kinase 2, mitochondrial
AK2
Functioni
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
UniRule annotationCatalytic activityi
- EC:2.7.4.3UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | AMPUniRule annotation | 1 | |
Binding sitei | 53 | AMPUniRule annotation | 1 | |
Binding sitei | 109 | AMPUniRule annotation | 1 | |
Binding sitei | 144 | ATPUniRule annotation | 1 | |
Binding sitei | 177 | AMPUniRule annotation | 1 | |
Binding sitei | 188 | AMPUniRule annotation | 1 | |
Binding sitei | 216 | ATP; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 32 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 74 – 76 | AMPUniRule annotation | 3 | |
Nucleotide bindingi | 102 – 105 | AMPUniRule annotation | 4 | |
Nucleotide bindingi | 153 – 154 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- adenylate kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- ADP biosynthetic process Source: UniProtKB-UniRule
- AMP metabolic process Source: UniProtKB-UniRule
- ATP metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-BTA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P08166 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenylate kinase 2, mitochondrialUniRule annotation (EC:2.7.4.3UniRule annotation)Short name: AK 2UniRule annotation Alternative name(s): ATP-AMP transphosphorylase 2UniRule annotation ATP:AMP phosphotransferaseUniRule annotation Adenylate monophosphate kinaseUniRule annotation Cleaved into the following chain: Adenylate kinase 2, mitochondrial, N-terminally processedUniRule annotation |
Gene namesi | Name:AK2UniRule annotation |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000017605 |
VGNCi | VGNC:25771, AK2 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial inner membrane Source: AgBase
- mitochondrial intermembrane space Source: UniProtKB-SubCell
- mitochondrion Source: AgBase
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423211 | 1 – 241 | Adenylate kinase 2, mitochondrialAdd BLAST | 241 | |
Initiator methioninei | Removed; alternateUniRule annotation1 Publication | |||
ChainiPRO_0000158916 | 2 – 241 | Adenylate kinase 2, mitochondrial, N-terminally processedAdd BLAST | 240 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Disulfide bondi | 44 ↔ 94 | UniRule annotation1 Publication | ||
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Modified residuei | 64 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 95 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 135 | PhosphoserineBy similarity | 1 | |
Modified residuei | 183 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 197 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinProteomic databases
PaxDbi | P08166 |
PeptideAtlasi | P08166 |
PRIDEi | P08166 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000017605, Expressed in rumen epithelium and 97 other tissues |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
IntActi | P08166, 1 interactor |
STRINGi | 9913.ENSBTAP00000023406 |
Structurei
Secondary structure
3D structure databases
SMRi | P08166 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08166 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 24 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 47 – 76 | NMPAdd BLAST | 30 | |
Regioni | 143 – 180 | LIDAdd BLAST | 38 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3078, Eukaryota |
GeneTreei | ENSGT00940000154576 |
HOGENOMi | CLU_032354_1_0_1 |
InParanoidi | P08166 |
OMAi | FHNRMRV |
OrthoDBi | 1004067at2759 |
TreeFami | TF300896 |
Family and domain databases
CDDi | cd01428, ADK, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 1 hit MF_03168, Adenylate_kinase_AK2, 1 hit |
InterProi | View protein in InterPro IPR006259, Adenyl_kin_sub IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR007862, Adenylate_kinase_lid-dom IPR028587, AK2 IPR027417, P-loop_NTPase |
PANTHERi | PTHR23359, PTHR23359, 1 hit |
Pfami | View protein in Pfam PF05191, ADK_lid, 1 hit |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01351, adk, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD
60 70 80 90 100
MLRAMVASGS ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL
110 120 130 140 150
DGFPRTVRQA EMLDDLMEKR KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS
160 170 180 190 200
GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD NKKALKIRLE AYHTQTTPLV
210 220 230 240
EYYSKRGIHS AIDASQTPDV VFASILAAFS KATCKDLVMF I
The sequence of this isoform differs from the canonical sequence as follows:
234-241: CKDLVMFI → S
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002789 | 234 – 241 | CKDLVMFI → S in isoform AK2B. 3 Publications | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16224 mRNA Translation: AAA30364.1 M16225 mRNA Translation: AAA30365.1 D90069 Genomic DNA Translation: BAA14110.1 D90069 Genomic DNA Translation: BAA14109.1 BT025476 mRNA Translation: ABF57432.1 BC112613 mRNA Translation: AAI12614.1 |
PIRi | B29792 JS0422 |
RefSeqi | NP_776314.1, NM_173889.1 [P08166-2] XP_005202939.1, XM_005202882.1 [P08166-1] |
Genome annotation databases
Ensembli | ENSBTAT00000023406; ENSBTAP00000023406; ENSBTAG00000017605 [P08166-2] |
GeneIDi | 280716 |
KEGGi | bta:280716 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16224 mRNA Translation: AAA30364.1 M16225 mRNA Translation: AAA30365.1 D90069 Genomic DNA Translation: BAA14110.1 D90069 Genomic DNA Translation: BAA14109.1 BT025476 mRNA Translation: ABF57432.1 BC112613 mRNA Translation: AAI12614.1 |
PIRi | B29792 JS0422 |
RefSeqi | NP_776314.1, NM_173889.1 [P08166-2] XP_005202939.1, XM_005202882.1 [P08166-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AK2 | X-ray | 1.92 | A | 2-233 | [»] | |
2AK2 | X-ray | 2.10 | A | 2-233 | [»] | |
SMRi | P08166 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P08166, 1 interactor |
STRINGi | 9913.ENSBTAP00000023406 |
Proteomic databases
PaxDbi | P08166 |
PeptideAtlasi | P08166 |
PRIDEi | P08166 |
Genome annotation databases
Ensembli | ENSBTAT00000023406; ENSBTAP00000023406; ENSBTAG00000017605 [P08166-2] |
GeneIDi | 280716 |
KEGGi | bta:280716 |
Organism-specific databases
CTDi | 204 |
VEuPathDBi | HostDB:ENSBTAG00000017605 |
VGNCi | VGNC:25771, AK2 |
Phylogenomic databases
eggNOGi | KOG3078, Eukaryota |
GeneTreei | ENSGT00940000154576 |
HOGENOMi | CLU_032354_1_0_1 |
InParanoidi | P08166 |
OMAi | FHNRMRV |
OrthoDBi | 1004067at2759 |
TreeFami | TF300896 |
Enzyme and pathway databases
Reactomei | R-BTA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P08166 |
Miscellaneous databases
EvolutionaryTracei | P08166 |
Gene expression databases
Bgeei | ENSBTAG00000017605, Expressed in rumen epithelium and 97 other tissues |
Family and domain databases
CDDi | cd01428, ADK, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 1 hit MF_03168, Adenylate_kinase_AK2, 1 hit |
InterProi | View protein in InterPro IPR006259, Adenyl_kin_sub IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR007862, Adenylate_kinase_lid-dom IPR028587, AK2 IPR027417, P-loop_NTPase |
PANTHERi | PTHR23359, PTHR23359, 1 hit |
Pfami | View protein in Pfam PF05191, ADK_lid, 1 hit |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01351, adk, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KAD2_BOVIN | |
Accessioni | P08166Primary (citable) accession number: P08166 Secondary accession number(s): P08167, Q2KIJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families