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Protein

Early growth response protein 1

Gene

Egr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator (PubMed:8413279). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (By similarity). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri311 – 335C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri341 – 363C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
Nerve growth factor-induced protein A1 Publication
Short name:
NGFI-A1 Publication
Transcription factor Zif268
Zinc finger protein Krox-24
Gene namesi
Name:Egr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2544 Egr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265I → F: Increases transcriptional activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471111 – 508Early growth response protein 1Add BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP08154

PTM databases

PhosphoSitePlusiP08154

Expressioni

Tissue specificityi

Detected in kidney thick ascending limbs and collecting ducts (at protein level).1 Publication

Inductioni

By growth factors (PubMed:2492104). Rapidly and transiently up-regulated in response to oschemia (PubMed:1859855).2 Publications

Gene expression databases

GenevisibleiP08154 RN

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei309Interaction with DNABy similarity1
Sitei320Interaction with DNABy similarity1
Sitei324Interaction with DNABy similarity1
Sitei330Interaction with DNABy similarity1
Sitei348Interaction with DNABy similarity1
Sitei352Interaction with DNABy similarity1
Sitei376Interaction with DNABy similarity1
Sitei380Interaction with DNABy similarity1
Sitei386Interaction with DNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026303

Structurei

3D structure databases

ProteinModelPortaliP08154
SMRiP08154
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati413 – 42018
Repeati421 – 42828
Repeati429 – 43638
Repeati437 – 44448
Repeati445 – 45258
Repeati453 – 46068
Repeati462 – 46877

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 4687 X 8 AA tandem repeats of [TS](2)-[FY]-[PS]-S-P-[GSAV]-XAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 56Gly/Ser-richAdd BLAST28

Domaini

Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers. The first, most N-terminal zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine.By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri311 – 335C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri341 – 363C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
HOGENOMiHOG000036856
HOVERGENiHBG003909
InParanoidiP08154
KOiK09203
OrthoDBiEOG091G06VX
PhylomeDBiP08154

Family and domain databases

InterProiView protein in InterPro
IPR021849 EGR
IPR021839 EGR1_C
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF11914 DUF3432, 1 hit
PF11928 DUF3446, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P08154-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDNYPKLEEM MLLSNGAPQF LGAAGTPEGS GGNNSSSSSS SSSGGGGGGG
60 70 80 90 100
SNSGSSAFNP QGEPSEQPYE HLTTESFSDI ALNNEKALVE TSYPSQTTRL
110 120 130 140 150
PPITYTGRFS LEPAPNSGNT LWPEPLFSLV SGLVSMTNPP TSSSSAPSPA
160 170 180 190 200
ASSSSSASQS PPLSCAVPSN DSSPIYSAAP TFPTPNTDIF PEPQSQAFPG
210 220 230 240 250
SAGTALQYPP PAYPATKGGF QVPMIPDYLF PQQQGDLSLG TPDQKPFQGL
260 270 280 290 300
ENRTQQPSLT PLSTIKAFAT QSGSQDLKAL NNTYQSQLIK PSRMRKYPNR
310 320 330 340 350
PSKTPPHERP YACPVESCDR RFSRSDELTR HIRIHTGQKP FQCRICMRNF
360 370 380 390 400
SRSDHLTTHI RTHTGEKPFA CDICGRKFAR SDERKRHTKI HLRQKDKKAD
410 420 430 440 450
KSVVASSAAS SLSSYPSPVA TSYPSPATTS FPSPVPTSYS SPGSSTYPSP
460 470 480 490 500
AHSGFPSPSV ATTYASVPPA FPAQVSTFQS AGVSNSFSTS TGLSDMTATF

SPRTIEIC
Length:508
Mass (Da):53,935
Last modified:January 1, 1990 - v2
Checksum:i3947BBA871BB8FA8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UHR3A0A0H2UHR3_RAT
Early growth response protein
Egr1 rCG_49327
536Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18416 mRNA Translation: AAA61927.1
J04154 Genomic DNA Translation: AAA60740.1
AY551092 mRNA Translation: AAS58455.1
PIRiA32225
RefSeqiNP_036683.1, NM_012551.2
UniGeneiRn.9096

Genome annotation databases

GeneIDi24330
KEGGirno:24330
UCSCiRGD:2544 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18416 mRNA Translation: AAA61927.1
J04154 Genomic DNA Translation: AAA60740.1
AY551092 mRNA Translation: AAS58455.1
PIRiA32225
RefSeqiNP_036683.1, NM_012551.2
UniGeneiRn.9096

3D structure databases

ProteinModelPortaliP08154
SMRiP08154
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026303

PTM databases

PhosphoSitePlusiP08154

Proteomic databases

PaxDbiP08154

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24330
KEGGirno:24330
UCSCiRGD:2544 rat

Organism-specific databases

CTDi1958
RGDi2544 Egr1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
HOGENOMiHOG000036856
HOVERGENiHBG003909
InParanoidiP08154
KOiK09203
OrthoDBiEOG091G06VX
PhylomeDBiP08154

Miscellaneous databases

PROiPR:P08154

Gene expression databases

GenevisibleiP08154 RN

Family and domain databases

InterProiView protein in InterPro
IPR021849 EGR
IPR021839 EGR1_C
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF11914 DUF3432, 1 hit
PF11928 DUF3446, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEGR1_RAT
AccessioniPrimary (citable) accession number: P08154
Secondary accession number(s): Q53YM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 1, 1990
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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