UniProtKB - P08153 (SWI5_YEAST)
Protein
Transcriptional factor SWI5
Gene
SWI5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis.
Miscellaneous
Present with 688 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 550 – 574 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 580 – 604 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 609 – 632 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 | |
DNA bindingi | 647 – 659 | A.T hookAdd BLAST | 13 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: SGD
- mediator complex binding Source: SGD
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: SGD
- sequence-specific DNA binding Source: SGD
GO - Biological processi
- positive regulation of mating type switching Source: SGD
- positive regulation of transcription involved in exit from mitosis, from RNA polymerase II promoter Source: SGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional factor SWI5 |
Gene namesi | Name:SWI5 Ordered Locus Names:YDR146C ORF Names:YD8358.03C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002553, SWI5 |
VEuPathDBi | FungiDB:YDR146C |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 522 | S → A: Constitutive nuclear entry; when associated with A-646 and A-664. 1 Publication | 1 | |
Mutagenesisi | 646 | S → A: Constitutive nuclear entry; when associated with A-522 and A-664. 1 Publication | 1 | |
Mutagenesisi | 664 | S → A: Constitutive nuclear entry; when associated with A-522 and A-646. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046855 | 1 – 709 | Transcriptional factor SWI5Add BLAST | 709 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 225 | PhosphoserineCombined sources | 1 | |
Modified residuei | 278 | PhosphoserineCombined sources | 1 | |
Modified residuei | 300 | PhosphoserineCombined sources | 1 | |
Modified residuei | 339 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 376 | PhosphoserineCombined sources | 1 | |
Modified residuei | 488 | PhosphoserineCombined sources | 1 | |
Modified residuei | 492 | PhosphoserineCombined sources | 1 | |
Modified residuei | 505 | PhosphoserineCombined sources | 1 | |
Modified residuei | 522 | Phosphoserine; by CDC28Combined sources1 Publication | 1 | |
Modified residuei | 646 | Phosphoserine; by CDC28Combined sources1 Publication | 1 | |
Modified residuei | 664 | Phosphoserine; by CDC281 Publication | 1 |
Post-translational modificationi
Cell cycle-dependent phosphorylation of three serine residues prevents SWI5 from entering the nucleus, and it accumulates in the cytoplasm. As a consequence of CDC28 kinase inactivation at the end of anaphase, the three serine residues are dephosphorylated and SWI5 enters the nucleus to activate transcription. It is then rapidly degraded. Threonine phosphorylation also seems to occur.
Phosphorylated by PHO85.
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P08153 |
PaxDbi | P08153 |
PRIDEi | P08153 |
PTM databases
iPTMneti | P08153 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32200, 424 interactors |
DIPi | DIP-1461N |
ELMi | P08153 |
IntActi | P08153, 20 interactors |
MINTi | P08153 |
STRINGi | 4932.YDR146C |
Miscellaneous databases
RNActi | P08153, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P08153 |
SMRi | P08153 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08153 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 635 – 659 | Nuclear localization signalSequence analysisAdd BLAST | 25 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 550 – 574 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 580 – 604 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 609 – 632 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000176710 |
HOGENOMi | CLU_021006_0_0_1 |
InParanoidi | P08153 |
OMAi | HNTERNI |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 2 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P08153-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD
60 70 80 90 100
LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ
110 120 130 140 150
SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS
160 170 180 190 200
MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE
210 220 230 240 250
LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS
260 270 280 290 300
PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS
310 320 330 340 350
PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN
360 370 380 390 400
VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN
410 420 430 440 450
TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA
460 470 480 490 500
RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK
510 520 530 540 550
YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF
560 570 580 590 600
ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH
610 620 630 640 650
KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR
660 670 680 690 700
GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM
VSPMKTNQR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 514 | E → K in AAU09695 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06978 Genomic DNA Translation: CAA30040.1 Z50046 Genomic DNA Translation: CAA90369.1 AY723778 Genomic DNA Translation: AAU09695.1 BK006938 Genomic DNA Translation: DAA11989.1 |
PIRi | S00342, TWBYS5 |
RefSeqi | NP_010430.1, NM_001180453.1 |
Genome annotation databases
EnsemblFungii | YDR146C_mRNA; YDR146C; YDR146C |
GeneIDi | 851724 |
KEGGi | sce:YDR146C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06978 Genomic DNA Translation: CAA30040.1 Z50046 Genomic DNA Translation: CAA90369.1 AY723778 Genomic DNA Translation: AAU09695.1 BK006938 Genomic DNA Translation: DAA11989.1 |
PIRi | S00342, TWBYS5 |
RefSeqi | NP_010430.1, NM_001180453.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NCS | NMR | - | A | 532-578 | [»] | |
1ZFD | NMR | - | A | 577-608 | [»] | |
BMRBi | P08153 | |||||
SMRi | P08153 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32200, 424 interactors |
DIPi | DIP-1461N |
ELMi | P08153 |
IntActi | P08153, 20 interactors |
MINTi | P08153 |
STRINGi | 4932.YDR146C |
PTM databases
iPTMneti | P08153 |
Proteomic databases
MaxQBi | P08153 |
PaxDbi | P08153 |
PRIDEi | P08153 |
Genome annotation databases
EnsemblFungii | YDR146C_mRNA; YDR146C; YDR146C |
GeneIDi | 851724 |
KEGGi | sce:YDR146C |
Organism-specific databases
SGDi | S000002553, SWI5 |
VEuPathDBi | FungiDB:YDR146C |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000176710 |
HOGENOMi | CLU_021006_0_0_1 |
InParanoidi | P08153 |
OMAi | HNTERNI |
Miscellaneous databases
EvolutionaryTracei | P08153 |
PROi | PR:P08153 |
RNActi | P08153, protein |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 2 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SWI5_YEAST | |
Accessioni | P08153Primary (citable) accession number: P08153 Secondary accession number(s): D6VSC9, E9P945 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 222 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names