UniProtKB - P08152 (EGR2_MOUSE)
Protein
E3 SUMO-protein ligase EGR2
Gene
Egr2
Organism
Mus musculus (Mouse)
Status
Functioni
Sequence-specific DNA-binding transcription factor (PubMed:1969796, PubMed:1674431, PubMed:11823429, PubMed:31852952). Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (PubMed:1969796, PubMed:1674431, PubMed:11823429, PubMed:31852952, PubMed:8093858). Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (PubMed:11823429). Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (PubMed:1969796, PubMed:8093858, PubMed:17938205). Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (PubMed:11823429, PubMed:1674431). Promotes the expression of HOXB3 in the rhombomere r5 and of HOXB3 in r3 and r5 in the hindbrain (PubMed:11823429, PubMed:8093858). Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (PubMed:7935840, PubMed:10068633). Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (PubMed:11509834). May play a role in adipogenesis, possibly by regulating the expression of CEBPB (PubMed:16054051).10 Publications
E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.By similarity
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 337 – 361 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 367 – 389 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 395 – 417 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- HMG box domain binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II activating transcription factor binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- SUMO ligase activity Source: ARUK-UCL
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- brain segmentation Source: MGI
- cellular response to organic substance Source: MGI
- facial nerve structural organization Source: UniProtKB
- fat cell differentiation Source: UniProtKB
- gene expression Source: MGI
- learning or memory Source: Ensembl
- motor neuron axon guidance Source: MGI
- myelination Source: MGI
- positive regulation of myelination Source: UniProtKB
- positive regulation of Schwann cell differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- protein export from nucleus Source: UniProtKB
- protein sumoylation Source: ARUK-UCL
- regulation of neuronal synaptic plasticity Source: Ensembl
- regulation of ossification Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to insulin Source: MGI
- rhombomere 3 development Source: MGI
- rhombomere 3 formation Source: MGI
- rhombomere 3 structural organization Source: UniProtKB
- rhombomere 5 formation Source: MGI
- rhombomere 5 structural organization Source: UniProtKB
- rhythmic behavior Source: MGI
- Schwann cell differentiation Source: UniProtKB
- skeletal muscle cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Transferase |
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-9031628, NGF-stimulated transcription |
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 SUMO-protein ligase EGR2 (EC:2.3.2.-By similarity)Alternative name(s): E3 SUMO-protein transferase ERG2Curated Early growth response protein 2 Short name: EGR-2 Zinc finger protein Krox-20 |
Gene namesi | Name:Egr2 Synonyms:Egr-2, Krox-20, Zfp-25 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95296, Egr2 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Failure to promote expression of the Hoxb3 reporter in rhombomere r5 in the hindbrain (PubMed:11823429). Changed morphology of the sciatic nerves, with a higher density of Schwann cells, and a reduction in major components of compacted myelin, including lipidic components, as well as myelin proteins Mpz and Mbp (PubMed:7935840). Schwann cells in the sciatic nerves exhibit increased expression of Scip, reduced expression of Mpz, elevated mitotic activity and increased apoptosis at postnatal day P12 (PubMed:10068633). Total absence of myelin along the axons of sciatic nerves at postnatal day P15 (PubMed:7935840). Does not seem to affect the myelination in the central nervous system (PubMed:7935840). Signs of atrophy in the jaw openener anterior digastric (AD) and mylohoid (MY) muscles at 15 dpc with smaller diameter fibers, fibers with triangular shape, increased amount of connective tissue surrounding the fibers, suggesting a lack of neural innervation (PubMed:11509834). Reduced volume of the delineated trigeminal motor nucleus and restructuring of the brainstem at 15 dpc (PubMed:11509834). Reduced volume in both AD and MY musculature and reduced milk indigestion after birth (PubMed:11509834).4 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 174 | Y → F: Abolishes interaction with WWP2; if associated with F-208. 1 Publication | 1 | |
Mutagenesisi | 208 | Y → F: Abolishes interaction with WWP2; if associated with F-174. 1 Publication | 1 | |
Mutagenesisi | 247 | K → R: Abolishes acetylation. 1 Publication | 1 | |
Mutagenesisi | 377 | S → A: Inhibits interaction with SFN. 1 Publication | 1 | |
Mutagenesisi | 408 | D → G: Reduces transcriptional regulatory activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047120 | 1 – 470 | E3 SUMO-protein ligase EGR2Add BLAST | 470 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 247 | N6-acetyllysine; by EP3001 Publication | 1 |
Post-translational modificationi
Ubiquitinated by WWP2 leading to proteasomal degradation.1 Publication
Acetylated at Lys-247. May be deacetylated by HDAC6, HDAC10 or SIRT1.1 Publication
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
PaxDbi | P08152 |
PRIDEi | P08152 |
ProteomicsDBi | 277805 [P08152-1] 277806 [P08152-2] |
PTM databases
iPTMneti | P08152 |
PhosphoSitePlusi | P08152 |
Expressioni
Tissue specificityi
Expressed mainly in the thymus.1 Publication
Developmental stagei
Before 8 dpc, expressed in the future rhombomere r3 at 0-3 somites, followed by expression in rhombomere r5 in 4-7 somites at 8 dpc, and maintained until 12 somites (PubMed:8093858). Expressed in migrating neural crest cells from r5/r6 (PubMed:8093858). Expressed in boundary cap cells that surround nerve exit points from the central nervous system at 10.5 dpc (PubMed:7935840, PubMed:8093858). Up to 14.5 dpc, expressed in motor and sensory roots of cranial and spinal nerves (PubMed:7935840). After 15.5 dpc, expressed in the entire peripheral nervous system (PubMed:7935840). Expressed in the embryonic nervous system (PubMed:17938205). Expressed in myelinating Schwann cells 2 weeks after birth (PubMed:7935840).3 Publications
Inductioni
Activated during G0/G1 transition in cultured cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000037868, Expressed in sciatic nerve and 224 other tissues |
Genevisiblei | P08152, MM |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP08152
With | #Exp. | IntAct |
---|---|---|
Pias2 - isoform 1 [Q8C5D8-1] | 5 | EBI-7070449,EBI-8064899 |
Ube2i [P63280] | 2 | EBI-7070449,EBI-80180 |
GO - Molecular functioni
- HMG box domain binding Source: MGI
- RNA polymerase II activating transcription factor binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199405, 5 interactors |
ELMi | P08152 |
IntActi | P08152, 5 interactors |
MINTi | P08152 |
STRINGi | 10090.ENSMUSP00000041053 |
Miscellaneous databases
RNActi | P08152, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 167 – 173 | Poly-Pro | 7 |
Sequence similaritiesi
Belongs to the EGR C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 337 – 361 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 367 – 389 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 395 – 417 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158394 |
InParanoidi | P08152 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P08152 |
TreeFami | TF318980 |
Family and domain databases
InterProi | View protein in InterPro IPR021849, EGR_N IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF11928, DUF3446, 1 hit PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform Long (identifier: P08152-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMTAKAVDKI PVTLSGFMHQ LPDSLYPVED LAASSVTIFP NGELGGPFDQ
60 70 80 90 100
MNGVAGDGMI NIDMTGEKRP LDLPYPSSFA PISAPRNQTF TYMGKFSIDP
110 120 130 140 150
QYPGASCYPE GIINIVSAGI LQGVTPPAST TASSSVTSAS PNPLATGPLG
160 170 180 190 200
VCTMSQTQPE LDHLYSPPPP PPPYSGCTGD LYQDPSAFLS PPSTTSTSSL
210 220 230 240 250
AYQPPPSYPS PKPAMDPGLI PMIPDYPGFF PSPCQRDPHG AAGPDRKPFP
260 270 280 290 300
CPLDSLRVPP PLTPLSTIRN FTLGGPGAGV TGPGASGGGE GPRLPGSGSA
310 320 330 340 350
AVTATPYNPH HLPLRPILRP RKYPNRPSKT PVHERPYPCP AEGCDRRFSR
360 370 380 390 400
SDELTRHIRI HTGHKPFQCR ICMRNFSRSD HLTTHIRTHT GEKPFACDYC
410 420 430 440 450
GRKFARSDER KRHTKIHLRQ KERKSSAPSA PPSAQSSASG PGGSQAGGSL
460 470
CGNSAIGGPL ASCTSRTRTP
Isoform Short (identifier: P08152-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-50: Missing.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z3K7 | D3Z3K7_MOUSE | E3 SUMO-protein ligase EGR2 | Egr2 | 412 | Annotation score: | ||
D3Z4G7 | D3Z4G7_MOUSE | E3 SUMO-protein ligase EGR2 | Egr2 | 236 | Annotation score: | ||
D3YYY2 | D3YYY2_MOUSE | E3 SUMO-protein ligase EGR2 | Egr2 | 281 | Annotation score: | ||
D3YXS2 | D3YXS2_MOUSE | E3 SUMO-protein ligase EGR2 | Egr2 | 72 | Annotation score: | ||
D3Z4X9 | D3Z4X9_MOUSE | E3 SUMO-protein ligase EGR2 | Egr2 | 36 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006864 | 1 – 50 | Missing in isoform Short. 1 PublicationAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24377, M24376 Genomic DNA Translation: AAA39379.1 M24377, M24376 Genomic DNA Translation: AAA39380.1 X06746 mRNA Translation: CAA29921.1 BC009093 mRNA Translation: AAH09093.1 M20759 Genomic DNA Translation: AAA39381.1 |
CCDSi | CCDS35927.2 [P08152-1] CCDS83706.1 [P08152-2] |
PIRi | A30136 S00256 |
RefSeqi | NP_001334387.1, NM_001347458.1 [P08152-2] NP_034248.2, NM_010118.3 [P08152-1] XP_006513272.1, XM_006513209.2 [P08152-2] XP_006513273.1, XM_006513210.2 XP_006513274.1, XM_006513211.2 XP_006513276.1, XM_006513213.3 XP_011241670.1, XM_011243368.2 [P08152-2] |
Genome annotation databases
Ensembli | ENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868 [P08152-1] ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868 [P08152-2] ENSMUST00000229738; ENSMUSP00000155634; ENSMUSG00000116458 [P08152-1] ENSMUST00000231046; ENSMUSP00000155032; ENSMUSG00000116458 [P08152-2] |
GeneIDi | 13654 |
KEGGi | mmu:13654 |
UCSCi | uc007flx.1, mouse [P08152-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24377, M24376 Genomic DNA Translation: AAA39379.1 M24377, M24376 Genomic DNA Translation: AAA39380.1 X06746 mRNA Translation: CAA29921.1 BC009093 mRNA Translation: AAH09093.1 M20759 Genomic DNA Translation: AAA39381.1 |
CCDSi | CCDS35927.2 [P08152-1] CCDS83706.1 [P08152-2] |
PIRi | A30136 S00256 |
RefSeqi | NP_001334387.1, NM_001347458.1 [P08152-2] NP_034248.2, NM_010118.3 [P08152-1] XP_006513272.1, XM_006513209.2 [P08152-2] XP_006513273.1, XM_006513210.2 XP_006513274.1, XM_006513211.2 XP_006513276.1, XM_006513213.3 XP_011241670.1, XM_011243368.2 [P08152-2] |
3D structure databases
SMRi | P08152 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199405, 5 interactors |
ELMi | P08152 |
IntActi | P08152, 5 interactors |
MINTi | P08152 |
STRINGi | 10090.ENSMUSP00000041053 |
PTM databases
iPTMneti | P08152 |
PhosphoSitePlusi | P08152 |
Proteomic databases
PaxDbi | P08152 |
PRIDEi | P08152 |
ProteomicsDBi | 277805 [P08152-1] 277806 [P08152-2] |
Protocols and materials databases
Antibodypediai | 14464, 499 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000048289; ENSMUSP00000041053; ENSMUSG00000037868 [P08152-1] ENSMUST00000105438; ENSMUSP00000101078; ENSMUSG00000037868 [P08152-2] ENSMUST00000229738; ENSMUSP00000155634; ENSMUSG00000116458 [P08152-1] ENSMUST00000231046; ENSMUSP00000155032; ENSMUSG00000116458 [P08152-2] |
GeneIDi | 13654 |
KEGGi | mmu:13654 |
UCSCi | uc007flx.1, mouse [P08152-1] |
Organism-specific databases
CTDi | 1959 |
MGIi | MGI:95296, Egr2 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158394 |
InParanoidi | P08152 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P08152 |
TreeFami | TF318980 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Reactomei | R-MMU-9031628, NGF-stimulated transcription |
Miscellaneous databases
BioGRID-ORCSi | 13654, 0 hits in 52 CRISPR screens |
ChiTaRSi | Egr2, mouse |
PROi | PR:P08152 |
RNActi | P08152, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037868, Expressed in sciatic nerve and 224 other tissues |
Genevisiblei | P08152, MM |
Family and domain databases
InterProi | View protein in InterPro IPR021849, EGR_N IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF11928, DUF3446, 1 hit PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EGR2_MOUSE | |
Accessioni | P08152Primary (citable) accession number: P08152 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families