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Protein

Alpha-amylase A

Gene

Amy-p

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: UniProtKB
  • alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase A (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-p
Synonyms:AmyA
ORF Names:CG18730
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000079 Amy-p

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000136419 – 494Alpha-amylase AAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PeptideAtlasiP08144
PRIDEiP08144

Expressioni

Gene expression databases

BgeeiFBgn0000079 Expressed in 7 organ(s), highest expression level in adult organism
ExpressionAtlasiP08144 baseline and differential
GenevisibleiP08144 DM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi70922, 2 interactors

Structurei

3D structure databases

ProteinModelPortaliP08144
SMRiP08144
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiP08144
KOiK01176
OrthoDBiEOG091G07V6
PhylomeDBiP08144

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P08144-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEEQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPYSSLDFN
160 170 180 190 200
PTCAISNYND ANEVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKNLNTDHGF ASGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA ITEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
TDTDQGPPTT DGHNIASPIF NSDNSCSGGW VCEHRWRQIY NMVAFRNTVG
410 420 430 440 450
SDEIQNWWDN GSNQISFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVTVGSD GRASINIGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,746
Last modified:August 1, 1988 - v1
Checksum:iB477D3B44754C298
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4LGS0A0A0B4LGS0_DROME
Alpha-amylase
Amy-p CG18730, Dmel_CG18730
494Annotation score:

Polymorphismi

At least 6 electrophoretic isozymes are known: Amy1, Amy2, Amy3, Amy4, Amy5 and Amy6. Strains J87 and KO123 express Amy2; KO140 and 1420#1 express Amy4; L16 expresses Amy5.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti11A → S in strain: KN-28. 1
Natural varianti16A → V in strain: KN-22 and KN-23. 1
Natural varianti71S → R in strain: KN-3, KN-9, KN-10 and L16. 1
Natural varianti121D → G in strain: TN256, 1420#1 and KO140. 1
Natural varianti121D → N in strain: KN-3, KN-9, KN-10 and L16. 1
Natural varianti138S → T in strain: JP-75, KN-7, KN-15, KN-17, KN-21 and KN-23. 1
Natural varianti144Y → H in strain: Canton-S. 1
Natural varianti156S → R in strain: 1420#1, KN-3, KN-9, KN-10, KO140, L16 and TN256. 1
Natural varianti181Y → N in strain: Canton-S. 1
Natural varianti231A → S in strain: JP-70, KN-17 and KN-21. 1
Natural varianti278D → N in strain: 1420#1, KO140, KN-3, KN-9, KN-10 and L16. 1
Natural varianti284T → I in strain: KN-21. 1
Natural varianti398T → A in strain: 1420#1, J87, JP-60, JP-70, JP-75, KO123, KO140, KN-9, KN-15, KN-21, L16 and TN256. 1
Natural varianti401S → L in strain: Berkeley. 1
Natural varianti403E → A in strain: J87, JP-60 and KO123. 1
Natural varianti465V → I in strain: 1420#1. 1
Natural varianti474S → Y in strain: KN-23. 1
Natural varianti476N → Y in strain: Berkeley, JP-1, JP-5, JP-15, JP-35, JP-55, JP-65, JP-84, KN-12, KN-22 and KN-27. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04569 Genomic DNA Translation: CAA28238.1
L22716 Genomic DNA Translation: AAA92226.1
L22719 Genomic DNA Translation: AAA92229.1
L22721 Genomic DNA Translation: AAA92231.1
L22725 Genomic DNA Translation: AAA92235.1
L22726 Genomic DNA Translation: AAA92239.1
L22729 Genomic DNA Translation: AAA92240.1
L22731 Genomic DNA Translation: AAA92234.1
L22733 Genomic DNA Translation: AAA92241.1
L22735 Genomic DNA Translation: AAA92236.1
AB042862 Genomic DNA Translation: BAB32503.1
AB042863 Genomic DNA Translation: BAB32504.1
AB042864 Genomic DNA Translation: BAB32505.1
AB042865 Genomic DNA Translation: BAB32506.1
AB042866 Genomic DNA Translation: BAB32507.1
AB042867 Genomic DNA Translation: BAB32508.1
AB042868 Genomic DNA Translation: BAB32509.1
AB042869 Genomic DNA Translation: BAB32510.1
AB042870 Genomic DNA Translation: BAB32511.1
AB042871 Genomic DNA Translation: BAB32512.1
AB042872 Genomic DNA Translation: BAB32513.1
AB042873 Genomic DNA Translation: BAB32514.1
AB042874 Genomic DNA Translation: BAB32515.1
AB042875 Genomic DNA Translation: BAB32516.1
AB042876 Genomic DNA Translation: BAB32517.1
AB042877 Genomic DNA Translation: BAB32518.1
AB042878 Genomic DNA Translation: BAB32519.1
AB042879 Genomic DNA Translation: BAB32520.1
AB042880 Genomic DNA Translation: BAB32521.1
AB042881 Genomic DNA Translation: BAB32522.1
AB042882 Genomic DNA Translation: BAB32523.1
AB042883 Genomic DNA Translation: BAB32524.1
AB042884 Genomic DNA Translation: BAB72090.1
AB042885 Genomic DNA Translation: BAB72091.1
AB042886 Genomic DNA Translation: BAB72092.1
AE013599 Genomic DNA Translation: AAF57896.1
AY051425 mRNA Translation: AAK92849.1
PIRiA25529
S58953
S58956
S58957
S58958
S58961
S58965
RefSeqiNP_001286518.1, NM_001299589.1
NP_536346.1, NM_080421.4
UniGeneiDm.20319

Genome annotation databases

GeneIDi47764
KEGGidme:Dmel_CG18730

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04569 Genomic DNA Translation: CAA28238.1
L22716 Genomic DNA Translation: AAA92226.1
L22719 Genomic DNA Translation: AAA92229.1
L22721 Genomic DNA Translation: AAA92231.1
L22725 Genomic DNA Translation: AAA92235.1
L22726 Genomic DNA Translation: AAA92239.1
L22729 Genomic DNA Translation: AAA92240.1
L22731 Genomic DNA Translation: AAA92234.1
L22733 Genomic DNA Translation: AAA92241.1
L22735 Genomic DNA Translation: AAA92236.1
AB042862 Genomic DNA Translation: BAB32503.1
AB042863 Genomic DNA Translation: BAB32504.1
AB042864 Genomic DNA Translation: BAB32505.1
AB042865 Genomic DNA Translation: BAB32506.1
AB042866 Genomic DNA Translation: BAB32507.1
AB042867 Genomic DNA Translation: BAB32508.1
AB042868 Genomic DNA Translation: BAB32509.1
AB042869 Genomic DNA Translation: BAB32510.1
AB042870 Genomic DNA Translation: BAB32511.1
AB042871 Genomic DNA Translation: BAB32512.1
AB042872 Genomic DNA Translation: BAB32513.1
AB042873 Genomic DNA Translation: BAB32514.1
AB042874 Genomic DNA Translation: BAB32515.1
AB042875 Genomic DNA Translation: BAB32516.1
AB042876 Genomic DNA Translation: BAB32517.1
AB042877 Genomic DNA Translation: BAB32518.1
AB042878 Genomic DNA Translation: BAB32519.1
AB042879 Genomic DNA Translation: BAB32520.1
AB042880 Genomic DNA Translation: BAB32521.1
AB042881 Genomic DNA Translation: BAB32522.1
AB042882 Genomic DNA Translation: BAB32523.1
AB042883 Genomic DNA Translation: BAB32524.1
AB042884 Genomic DNA Translation: BAB72090.1
AB042885 Genomic DNA Translation: BAB72091.1
AB042886 Genomic DNA Translation: BAB72092.1
AE013599 Genomic DNA Translation: AAF57896.1
AY051425 mRNA Translation: AAK92849.1
PIRiA25529
S58953
S58956
S58957
S58958
S58961
S58965
RefSeqiNP_001286518.1, NM_001299589.1
NP_536346.1, NM_080421.4
UniGeneiDm.20319

3D structure databases

ProteinModelPortaliP08144
SMRiP08144
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70922, 2 interactors

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PeptideAtlasiP08144
PRIDEiP08144

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi47764
KEGGidme:Dmel_CG18730

Organism-specific databases

CTDi47764
FlyBaseiFBgn0000079 Amy-p

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiP08144
KOiK01176
OrthoDBiEOG091G07V6
PhylomeDBiP08144

Enzyme and pathway databases

ReactomeiR-DME-189085 Digestion of dietary carbohydrate

Miscellaneous databases

GenomeRNAii47764
PROiPR:P08144

Gene expression databases

BgeeiFBgn0000079 Expressed in 7 organ(s), highest expression level in adult organism
ExpressionAtlasiP08144 baseline and differential
GenevisibleiP08144 DM

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYA_DROME
AccessioniPrimary (citable) accession number: P08144
Secondary accession number(s): Q27582
, Q27583, Q27584, Q27887, Q961R0, Q969D2, Q9BH42, Q9BH55, Q9BPT4, Q9BPT5, Q9BPT6, Q9BPT7, Q9BPT8, Q9BPT9, Q9BPU0, Q9BPU1, Q9V7Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: September 12, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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