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Protein

Endoplasmin

Gene

Hsp90b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPBy similarity1
Binding sitei149ATPBy similarity1
Binding sitei162ATPBy similarity1
Binding sitei168ATPBy similarity1
Binding sitei199ATP; via amide nitrogenBy similarity1
Binding sitei448ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-3000480 Scavenging by Class A Receptors
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-6785807 Interleukin-4 and Interleukin-13 signaling
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Endoplasmic reticulum resident protein 99
Short name:
ERp99
Heat shock protein 90 kDa beta member 1
Polymorphic tumor rejection antigen 1
Tumor rejection antigen gp96
Gene namesi
Name:Hsp90b1
Synonyms:Grp94, Tra-1, Tra1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:98817 Hsp90b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103E → A: Loss of CNPY3-binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3425396

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001359922 – 802EndoplasminAdd BLAST781

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei64PhosphoserineBy similarity1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi138Interchain
Modified residuei172PhosphoserineBy similarity1
Glycosylationi217N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei306PhosphoserineBy similarity1
Modified residuei403PhosphoserineBy similarity1
Modified residuei404N6-succinyllysineCombined sources1
Glycosylationi445N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei447PhosphoserineBy similarity1
Modified residuei479N6-acetyllysineCombined sources1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi502N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei633N6-succinyllysineCombined sources1
Modified residuei785PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP08113
PaxDbiP08113
PeptideAtlasiP08113
PRIDEiP08113

2D gel databases

REPRODUCTION-2DPAGEiP08113
SWISS-2DPAGEiP08113

PTM databases

iPTMnetiP08113
PhosphoSitePlusiP08113
SwissPalmiP08113

Miscellaneous databases

PMAP-CutDBiP08113

Expressioni

Gene expression databases

BgeeiENSMUSG00000020048 Expressed in 281 organ(s), highest expression level in female gonad
CleanExiMM_HSP90B1
ExpressionAtlasiP08113 baseline and differential
GenevisibleiP08113 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with OS9 (By similarity). Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with TLR4, TLR9 and TLR11, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner. Interacts with METTL23 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204301, 12 interactors
CORUMiP08113
DIPiDIP-32352N
IntActiP08113, 18 interactors
MINTiP08113
STRINGi10090.ENSMUSP00000020238

Structurei

3D structure databases

ProteinModelPortaliP08113
SMRiP08113
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi799 – 802Prevents secretion from ER4

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiP08113
KOiK09487
OMAiMRRMKEM
OrthoDBiEOG091G0270
PhylomeDBiP08113
TreeFamiTF105969

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00298 HSP90, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P08113-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ
60 70 80 90 100
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF
110 120 130 140 150
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT
160 170 180 190 200
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY
210 220 230 240 250
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV
260 270 280 290 300
LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDEAAK
310 320 330 340 350
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV
360 370 380 390 400
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE
410 420 430 440 450
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET
460 470 480 490 500
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH
510 520 530 540 550
SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE
560 570 580 590 600
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE
610 620 630 640 650
SEKTKESREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS
660 670 680 690 700
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR
710 720 730 740 750
IKEDEDDKTV MDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID
760 770 780 790 800
PEAQVEEEPE EEPEDTSEDA EDSEQDEGEE MDAGTEEEEE ETEKESTEKD

EL
Length:802
Mass (Da):92,476
Last modified:April 1, 1990 - v2
Checksum:iBDF33B9BD86BFC5F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7C312F7C312_MOUSE
Endoplasmin
Hsp90b1
373Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03297 mRNA Translation: AAA37573.1
M29652 mRNA Translation: AAA37743.1
M16370 mRNA Translation: AAA40023.1
CCDSiCCDS36019.1
PIRiA29317
RefSeqiNP_035761.1, NM_011631.1
UniGeneiMm.87773

Genome annotation databases

EnsembliENSMUST00000020238; ENSMUSP00000020238; ENSMUSG00000020048
GeneIDi22027
KEGGimmu:22027
UCSCiuc007gqi.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03297 mRNA Translation: AAA37573.1
M29652 mRNA Translation: AAA37743.1
M16370 mRNA Translation: AAA40023.1
CCDSiCCDS36019.1
PIRiA29317
RefSeqiNP_035761.1, NM_011631.1
UniGeneiMm.87773

3D structure databases

ProteinModelPortaliP08113
SMRiP08113
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204301, 12 interactors
CORUMiP08113
DIPiDIP-32352N
IntActiP08113, 18 interactors
MINTiP08113
STRINGi10090.ENSMUSP00000020238

Chemistry databases

ChEMBLiCHEMBL3425396

PTM databases

iPTMnetiP08113
PhosphoSitePlusiP08113
SwissPalmiP08113

2D gel databases

REPRODUCTION-2DPAGEiP08113
SWISS-2DPAGEiP08113

Proteomic databases

EPDiP08113
PaxDbiP08113
PeptideAtlasiP08113
PRIDEiP08113

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020238; ENSMUSP00000020238; ENSMUSG00000020048
GeneIDi22027
KEGGimmu:22027
UCSCiuc007gqi.1 mouse

Organism-specific databases

CTDi7184
MGIiMGI:98817 Hsp90b1

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiP08113
KOiK09487
OMAiMRRMKEM
OrthoDBiEOG091G0270
PhylomeDBiP08113
TreeFamiTF105969

Enzyme and pathway databases

ReactomeiR-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-3000480 Scavenging by Class A Receptors
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-6785807 Interleukin-4 and Interleukin-13 signaling
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiHsp90b1 mouse
PMAP-CutDBiP08113
PROiPR:P08113
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020048 Expressed in 281 organ(s), highest expression level in female gonad
CleanExiMM_HSP90B1
ExpressionAtlasiP08113 baseline and differential
GenevisibleiP08113 MM

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENPL_MOUSE
AccessioniPrimary (citable) accession number: P08113
Secondary accession number(s): P11427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: November 7, 2018
This is version 192 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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