UniProtKB - P08103 (HCK_MOUSE)
Tyrosine-protein kinase HCK
Hck
Functioni
Catalytic activityi
- ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]PROSITE-ProRule annotation1 PublicationEC:2.7.10.2PROSITE-ProRule annotation1 Publication
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 288 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 379 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 266 – 274 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- non-membrane spanning protein tyrosine kinase activity Source: GO_Central
- phosphotyrosine residue binding Source: MGI
- protein tyrosine kinase activity Source: UniProtKB
- signaling receptor binding Source: GO_Central
GO - Biological processi
- cell differentiation Source: GO_Central
- defense response to Gram-positive bacterium Source: MGI
- exocytosis Source: UniProtKB-KW
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- negative regulation of apoptotic process Source: MGI
- peptidyl-tyrosine autophosphorylation Source: GO_Central
- peptidyl-tyrosine phosphorylation Source: UniProtKB
- phagocytosis Source: MGI
- positive regulation of actin cytoskeleton reorganization Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- regulation of cell population proliferation Source: GO_Central
- regulation of cell shape Source: UniProtKB
- regulation of DNA-binding transcription factor activity Source: MGI
- regulation of phagocytosis Source: UniProtKB
- regulation of podosome assembly Source: UniProtKB
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase, Tyrosine-protein kinase |
Biological process | Exocytosis, Immunity, Inflammatory response, Innate immunity, Phagocytosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2 3474 |
Reactomei | R-MMU-2029481 FCGR activation R-MMU-912631 Regulation of signaling by CBL |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein kinase HCK (EC:2.7.10.2)Alternative name(s): B-cell/myeloid kinase Short name: BMK Hematopoietic cell kinase Hemopoietic cell kinase p56-HCK/p59-HCK |
Gene namesi | Name:Hck |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96052 Hck |
Subcellular locationi
Cytosol
- cytosol By similarity
Other locations
- secretory vesicle By similarity
Lysosome
- Lysosome By similarity
Plasma membrane
- podosome membrane By similarity; Lipid-anchor By similarity
Cytosol
- cytosol By similarity
Other locations
- Membrane ; Lipid-anchor
- caveola By similarity
Note: Associated with specialized secretory lysosomes called azurophil granules. A fraction of this isoform is found in the cytoplasm, some of this fraction is myristoylated (By similarity).By similarity
Lysosome
- Lysosome By similarity
Golgi apparatus
- Golgi apparatus By similarity
Plasma membrane
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus By similarity
Other locations
- caveola By similarity; Lipid-anchor By similarity
- focal adhesion By similarity
- Cytoplasmic vesicle By similarity
Note: 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions (By similarity).By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Lysosome
- lysosome Source: UniProtKB
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- caveola Source: MGI
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cell projection Source: UniProtKB-KW
- focal adhesion Source: MGI
- transport vesicle Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Lysosome, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 409 | Y → F: Reduced autophosphorylation. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogenePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | ||||
ChainiPRO_0000024435 | 2 – 524 | Tyrosine-protein kinase HCKAdd BLAST | 523 | ||
Isoform 2 (identifier: P08103-2) | |||||
Initiator methioninei | RemovedBy similarity |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | ||
Modified residuei | 50 | Phosphotyrosine; by autocatalysis1 Publication | 1 | ||
Modified residuei | 200 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 207 | PhosphotyrosineCombined sources | 1 | ||
Modified residuei | 409 | Phosphotyrosine; by autocatalysis1 Publication | 1 | ||
Modified residuei | 460 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 520 | PhosphotyrosineCombined sources | 1 | ||
Isoform 2 (identifier: P08103-2) | |||||
Lipidationi | 2 | N-myristoyl glycine By similarityBy similarity | 1 | ||
Lipidationi | 3 | S-palmitoyl cysteine By similarityBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P08103 |
MaxQBi | P08103 |
PaxDbi | P08103 |
PeptideAtlasi | P08103 |
PRIDEi | P08103 |
PTM databases
iPTMneti | P08103 |
PhosphoSitePlusi | P08103 |
SwissPalmi | P08103 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000003283 Expressed in 144 organ(s), highest expression level in spleen |
ExpressionAtlasi | P08103 baseline and differential |
Genevisiblei | P08103 MM |
Interactioni
Subunit structurei
Interacts with ADAM15.
Interacts with FASLG.
Interacts with ARRB1 and ARRB2.
Interacts with FCGR1A; the interaction may be indirect.
Interacts with IL6ST.
Interacts (via SH3 domain) with ELMO1.
Interacts (via SH3 domain) with TP73.
Interacts with YAP1.
Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1 (By similarity).
Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated).
Interacts with CBL.
Interacts with VAV1, WAS and RAPGEF1.
Interacts (via SH3 domain) with WDCP (By similarity).
By similarity6 PublicationsBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
P42768 | 3 | EBI-6248894,EBI-346375 | From Homo sapiens. |
GO - Molecular functioni
- phosphotyrosine residue binding Source: MGI
- signaling receptor binding Source: GO_Central
Protein-protein interaction databases
BioGridi | 200249, 5 interactors |
IntActi | P08103, 5 interactors |
MINTi | P08103 |
STRINGi | 10090.ENSMUSP00000003370 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 136 | SH3PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 142 – 239 | SH2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 260 – 513 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Sequence similaritiesi
Keywords - Domaini
SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG0197 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000158738 |
HOGENOMi | HOG000233858 |
InParanoidi | P08103 |
KOi | K08893 |
OMAi | GEWWQAR |
OrthoDBi | 539311at2759 |
PhylomeDBi | P08103 |
Family and domain databases
CDDi | cd10363 SH2_Src_HCK, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR035851 HCK_SH2 IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE |
SMARTi | View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGGRSSCEDP GCPRSEGRAP RMGCVKSRFL RDGSKASKTE PSANQKGPVY
60 70 80 90 100
VPDPTSSSKL GPNNSNSMPP GFVEGSEDTI VVALYDYEAI HREDLSFQKG
110 120 130 140 150
DQMVVLEEAG EWWKARSLAT KKEGYIPSNY VARVNSLETE EWFFKGISRK
160 170 180 190 200
DAERHLLAPG NMLGSFMIRD SETTKGSYSL SVRDFDPQHG DTVKHYKIRT
210 220 230 240 250
LDSGGFYISP RSTFSSLQEL VLHYKKGKDG LCQKLSVPCV SPKPQKPWEK
260 270 280 290 300
DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF
310 320 330 340 350
LAEANLMKSL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK
360 370 380 390 400
QPLPKLIDFS AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL
410 420 430 440 450
ARIIEDNEYT AREGAKFPIK WTAPEAINFG SFTIKSDVWS FGILLMEIVT
460 470 480 490 500
YGRIPYPGMS NPEVIRALEH GYRMPRPDNC PEELYNIMIR CWKNRPEERP
510 520
TFEYIQSVLD DFYTATESQY QQQP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6UND7 | F6UND7_MOUSE | Tyrosine-protein kinase | Hck | 524 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018859 | 1 – 21 | Missing in isoform 2. CuratedAdd BLAST | 21 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00487 mRNA Translation: CAA68544.1 Sequence problems. J03023 mRNA Translation: AAA37305.1 Sequence problems. AK149736 mRNA Translation: BAE29054.1 Sequence problems. AK150290 mRNA Translation: BAE29445.1 Sequence problems. AK150709 mRNA Translation: BAE29787.1 Sequence problems. AK155975 mRNA Translation: BAE33532.1 Sequence problems. AK165315 mRNA Translation: BAE38133.1 Sequence problems. BC010478 mRNA Translation: AAH10478.2 |
CCDSi | CCDS38284.1 [P08103-1] CCDS50756.1 [P08103-2] |
PIRi | A27282 TVMSHC |
RefSeqi | NP_001165588.1, NM_001172117.1 [P08103-2] NP_034537.2, NM_010407.4 [P08103-1] |
Genome annotation databases
Ensembli | ENSMUST00000109799; ENSMUSP00000105423; ENSMUSG00000003283 [P08103-2] ENSMUST00000189688; ENSMUSP00000141030; ENSMUSG00000003283 [P08103-2] ENSMUST00000191431; ENSMUSP00000139988; ENSMUSG00000003283 [P08103-1] |
GeneIDi | 15162 |
KEGGi | mmu:15162 |
UCSCi | uc008nhc.3 mouse [P08103-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00487 mRNA Translation: CAA68544.1 Sequence problems. J03023 mRNA Translation: AAA37305.1 Sequence problems. AK149736 mRNA Translation: BAE29054.1 Sequence problems. AK150290 mRNA Translation: BAE29445.1 Sequence problems. AK150709 mRNA Translation: BAE29787.1 Sequence problems. AK155975 mRNA Translation: BAE33532.1 Sequence problems. AK165315 mRNA Translation: BAE38133.1 Sequence problems. BC010478 mRNA Translation: AAH10478.2 |
CCDSi | CCDS38284.1 [P08103-1] CCDS50756.1 [P08103-2] |
PIRi | A27282 TVMSHC |
RefSeqi | NP_001165588.1, NM_001172117.1 [P08103-2] NP_034537.2, NM_010407.4 [P08103-1] |
3D structure databases
SMRi | P08103 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 200249, 5 interactors |
IntActi | P08103, 5 interactors |
MINTi | P08103 |
STRINGi | 10090.ENSMUSP00000003370 |
PTM databases
iPTMneti | P08103 |
PhosphoSitePlusi | P08103 |
SwissPalmi | P08103 |
Proteomic databases
jPOSTi | P08103 |
MaxQBi | P08103 |
PaxDbi | P08103 |
PeptideAtlasi | P08103 |
PRIDEi | P08103 |
Genome annotation databases
Ensembli | ENSMUST00000109799; ENSMUSP00000105423; ENSMUSG00000003283 [P08103-2] ENSMUST00000189688; ENSMUSP00000141030; ENSMUSG00000003283 [P08103-2] ENSMUST00000191431; ENSMUSP00000139988; ENSMUSG00000003283 [P08103-1] |
GeneIDi | 15162 |
KEGGi | mmu:15162 |
UCSCi | uc008nhc.3 mouse [P08103-1] |
Organism-specific databases
CTDi | 3055 |
MGIi | MGI:96052 Hck |
Phylogenomic databases
eggNOGi | KOG0197 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000158738 |
HOGENOMi | HOG000233858 |
InParanoidi | P08103 |
KOi | K08893 |
OMAi | GEWWQAR |
OrthoDBi | 539311at2759 |
PhylomeDBi | P08103 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2 3474 |
Reactomei | R-MMU-2029481 FCGR activation R-MMU-912631 Regulation of signaling by CBL |
Miscellaneous databases
PROi | PR:P08103 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003283 Expressed in 144 organ(s), highest expression level in spleen |
ExpressionAtlasi | P08103 baseline and differential |
Genevisiblei | P08103 MM |
Family and domain databases
CDDi | cd10363 SH2_Src_HCK, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR035851 HCK_SH2 IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE |
SMARTi | View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HCK_MOUSE | |
Accessioni | P08103Primary (citable) accession number: P08103 Secondary accession number(s): Q0VH03, Q3UD17 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | October 16, 2019 | |
This is version 202 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot