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Protein

Lactotransferrin

Gene

Ltf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (By similarity).By similarity
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi78Iron 1PROSITE-ProRule annotation1
Active sitei91PROSITE-ProRule annotation1
Metal bindingi110Iron 1PROSITE-ProRule annotation1
Binding sitei135Carbonate 1PROSITE-ProRule annotation1
Binding sitei139Carbonate 1PROSITE-ProRule annotation1
Binding sitei141Carbonate 1; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei142Carbonate 1; via amide nitrogenPROSITE-ProRule annotation1
Metal bindingi210Iron 1PROSITE-ProRule annotation1
Metal bindingi271Iron 1PROSITE-ProRule annotation1
Active sitei277NucleophilePROSITE-ProRule annotation1
Metal bindingi413Iron 2PROSITE-ProRule annotation1
Metal bindingi451Iron 2PROSITE-ProRule annotation1
Binding sitei477Carbonate 2PROSITE-ProRule annotation1
Binding sitei481Carbonate 2PROSITE-ProRule annotation1
Binding sitei483Carbonate 2; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei484Carbonate 2; via amide nitrogenPROSITE-ProRule annotation1
Metal bindingi544Iron 2PROSITE-ProRule annotation1
Metal bindingi613Iron 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processImmunity, Ion transport, Iron transport, Osteogenesis, Transport
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6799990 Metal sequestration by antimicrobial proteins
R-MMU-6803157 Antimicrobial peptides

Protein family/group databases

MEROPSiS60.001

Names & Taxonomyi

Protein namesi
Recommended name:
Lactotransferrin (EC:3.4.21.-)
Short name:
Lactoferrin
Gene namesi
Name:Ltf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:96837 Ltf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000003573720 – 707LactotransferrinAdd BLAST688

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 63PROSITE-ProRule annotation
Disulfide bondi37 ↔ 54PROSITE-ProRule annotation
Glycosylationi118N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi133 ↔ 216PROSITE-ProRule annotation
Disulfide bondi175 ↔ 191PROSITE-ProRule annotation
Disulfide bondi188 ↔ 199PROSITE-ProRule annotation
Disulfide bondi249 ↔ 263PROSITE-ProRule annotation
Disulfide bondi366 ↔ 398PROSITE-ProRule annotation
Disulfide bondi376 ↔ 389PROSITE-ProRule annotation
Disulfide bondi423 ↔ 702PROSITE-ProRule annotation
Disulfide bondi443 ↔ 665PROSITE-ProRule annotation
Disulfide bondi475 ↔ 550PROSITE-ProRule annotation
Glycosylationi494N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi499 ↔ 693PROSITE-ProRule annotation
Disulfide bondi509 ↔ 523PROSITE-ProRule annotation
Disulfide bondi520 ↔ 533PROSITE-ProRule annotation
Disulfide bondi591 ↔ 605PROSITE-ProRule annotation
Disulfide bondi643 ↔ 648PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP08071
PaxDbiP08071
PeptideAtlasiP08071
PRIDEiP08071

PTM databases

PhosphoSitePlusiP08071
SwissPalmiP08071

Expressioni

Gene expression databases

BgeeiENSMUSG00000032496 Expressed in 111 organ(s), highest expression level in trachea
CleanExiMM_LTF
ExpressionAtlasiP08071 baseline and differential
GenevisibleiP08071 MM

Interactioni

Subunit structurei

Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP08071, 1 interactor
MINTiP08071
STRINGi10090.ENSMUSP00000035077

Structurei

3D structure databases

ProteinModelPortaliP08071
SMRiP08071
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 351Transferrin-like 1PROSITE-ProRule annotationAdd BLAST328
Domaini363 – 692Transferrin-like 2PROSITE-ProRule annotationAdd BLAST330

Sequence similaritiesi

Belongs to the transferrin family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IEAI Eukaryota
ENOG410XQ36 LUCA
GeneTreeiENSGT00390000001619
HOGENOMiHOG000043759
HOVERGENiHBG000055
InParanoidiP08071
KOiK17283
OMAiNKCAFSS
OrthoDBiEOG091G0242
TreeFamiTF324013

Family and domain databases

InterProiView protein in InterPro
IPR030684 Lactotransferrin
IPR016357 Transferrin
IPR001156 Transferrin-like_dom
IPR018195 Transferrin_Fe_BS
PANTHERiPTHR11485:SF33 PTHR11485:SF33, 1 hit
PfamiView protein in Pfam
PF00405 Transferrin, 2 hits
PIRSFiPIRSF500683 Lactotransferrin, 1 hit
PIRSF002549 Transferrin, 1 hit
PRINTSiPR00422 TRANSFERRIN
SMARTiView protein in SMART
SM00094 TR_FER, 2 hits
PROSITEiView protein in PROSITE
PS00205 TRANSFERRIN_LIKE_1, 1 hit
PS00206 TRANSFERRIN_LIKE_2, 2 hits
PS00207 TRANSFERRIN_LIKE_3, 2 hits
PS51408 TRANSFERRIN_LIKE_4, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

P08071-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLLIPSLIF LEALGLCLAK ATTVQWCAVS NSEEEKCLRW QNEMRKVGGP
60 70 80 90 100
PLSCVKKSST RQCIQAIVTN RADAMTLDGG TMFDAGKPPY KLRPVAAEVY
110 120 130 140 150
GTKEQPRTHY YAVAVVKNSS NFHLNQLQGL RSCHTGIGRS AGWKIPIGTL
160 170 180 190 200
RPYLNWNGPP ASLEEAVSKF FSKSCVPGAQ KDRFPNLCSS CAGTGANKCA
210 220 230 240 250
SSPEEPYSGY AGALRCLRDN AGDVAFTRGS TVFEELPNKA ERDQYKLLCP
260 270 280 290 300
DNTWKPVTEY KECHLAQVPS HAVVSRSTND KEEAIWELLR QSQEKFGKKQ
310 320 330 340 350
ASGFQLFASP SGQKDLLFKE SAIGFVRVPQ KVDVGLYLTF SYTTSIQNLN
360 370 380 390 400
KKQQDVIASK ARVTWCAVGS EEKRKCDQWN RASRGRVTCI SFPTTEDCIV
410 420 430 440 450
AIMKGDADAM SLDGGYIYTA GKCGLVPVLA ENQKSSKSNG LDCVNRPVEG
460 470 480 490 500
YLAVAAVRRE DAGFTWSSLR GKKSCHTAVD RTAGWNIPMG LLANQTRSCK
510 520 530 540 550
FNEFFSQSCA PGADPKSNLC ALCIGDEKGE NKCAPNSKER YQGYTGALRC
560 570 580 590 600
LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT
610 620 630 640 650
EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF
660 670 680 690 700
QSKTKNLLFN DNTECLAKIP GKTTSEKYLG KEYVIATERL KQCSSSPLLE

ACAFLTQ
Length:707
Mass (Da):77,838
Last modified:July 27, 2011 - v4
Checksum:iE1B32F5FD8748A0F
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JGX1A0A0G2JGX1_MOUSE
Lactotransferrin
Ltf
78Annotation score:
A0A0G2JGN1A0A0G2JGN1_MOUSE
Lactotransferrin
Ltf
122Annotation score:
A0A0G2JFM6A0A0G2JFM6_MOUSE
Lactotransferrin
Ltf
184Annotation score:
A0A0G2JEA3A0A0G2JEA3_MOUSE
Lactotransferrin
Ltf
127Annotation score:
A0A0G2JDN0A0A0G2JDN0_MOUSE
Lactotransferrin
Ltf
95Annotation score:
A0A0G2JEE4A0A0G2JEE4_MOUSE
Lactotransferrin
Ltf
56Annotation score:
A0A0G2JFX4A0A0G2JFX4_MOUSE
Lactotransferrin
Ltf
19Annotation score:
A0A0G2JDK4A0A0G2JDK4_MOUSE
Lactotransferrin
Ltf
12Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 2MR → IQG in AAA40525 (PubMed:3611056).Curated2
Sequence conflicti25Q → R in AAA40525 (PubMed:3611056).Curated1
Sequence conflicti25Q → R in AAH06904 (PubMed:15489334).Curated1
Sequence conflicti82M → L in BAA13633 (Ref. 2) Curated1
Sequence conflicti359S → T in BAA13633 (Ref. 2) Curated1
Sequence conflicti382A → D in AAA40525 (PubMed:3611056).Curated1
Sequence conflicti449E → G in BAA13633 (Ref. 2) Curated1
Sequence conflicti629L → V in AAA40525 (PubMed:3611056).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03298 mRNA Translation: AAA40525.1
D88510 mRNA Translation: BAA13633.1
AK036491 mRNA Translation: BAC29450.1
AK144556 mRNA Translation: BAE25936.1
AK151822 mRNA Translation: BAE30719.1
BC006904 mRNA Translation: AAH06904.1
M74778 Genomic DNA Translation: AAA39427.1
CCDSiCCDS23581.1
PIRiA28438
RefSeqiNP_032548.2, NM_008522.3
UniGeneiMm.282359

Genome annotation databases

EnsembliENSMUST00000035077; ENSMUSP00000035077; ENSMUSG00000032496
GeneIDi17002
KEGGimmu:17002
UCSCiuc009rvj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03298 mRNA Translation: AAA40525.1
D88510 mRNA Translation: BAA13633.1
AK036491 mRNA Translation: BAC29450.1
AK144556 mRNA Translation: BAE25936.1
AK151822 mRNA Translation: BAE30719.1
BC006904 mRNA Translation: AAH06904.1
M74778 Genomic DNA Translation: AAA39427.1
CCDSiCCDS23581.1
PIRiA28438
RefSeqiNP_032548.2, NM_008522.3
UniGeneiMm.282359

3D structure databases

ProteinModelPortaliP08071
SMRiP08071
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08071, 1 interactor
MINTiP08071
STRINGi10090.ENSMUSP00000035077

Protein family/group databases

MEROPSiS60.001

PTM databases

PhosphoSitePlusiP08071
SwissPalmiP08071

Proteomic databases

MaxQBiP08071
PaxDbiP08071
PeptideAtlasiP08071
PRIDEiP08071

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035077; ENSMUSP00000035077; ENSMUSG00000032496
GeneIDi17002
KEGGimmu:17002
UCSCiuc009rvj.1 mouse

Organism-specific databases

CTDi4057
MGIiMGI:96837 Ltf

Phylogenomic databases

eggNOGiENOG410IEAI Eukaryota
ENOG410XQ36 LUCA
GeneTreeiENSGT00390000001619
HOGENOMiHOG000043759
HOVERGENiHBG000055
InParanoidiP08071
KOiK17283
OMAiNKCAFSS
OrthoDBiEOG091G0242
TreeFamiTF324013

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6799990 Metal sequestration by antimicrobial proteins
R-MMU-6803157 Antimicrobial peptides

Miscellaneous databases

ChiTaRSiLtf mouse
PROiPR:P08071
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032496 Expressed in 111 organ(s), highest expression level in trachea
CleanExiMM_LTF
ExpressionAtlasiP08071 baseline and differential
GenevisibleiP08071 MM

Family and domain databases

InterProiView protein in InterPro
IPR030684 Lactotransferrin
IPR016357 Transferrin
IPR001156 Transferrin-like_dom
IPR018195 Transferrin_Fe_BS
PANTHERiPTHR11485:SF33 PTHR11485:SF33, 1 hit
PfamiView protein in Pfam
PF00405 Transferrin, 2 hits
PIRSFiPIRSF500683 Lactotransferrin, 1 hit
PIRSF002549 Transferrin, 1 hit
PRINTSiPR00422 TRANSFERRIN
SMARTiView protein in SMART
SM00094 TR_FER, 2 hits
PROSITEiView protein in PROSITE
PS00205 TRANSFERRIN_LIKE_1, 1 hit
PS00206 TRANSFERRIN_LIKE_2, 2 hits
PS00207 TRANSFERRIN_LIKE_3, 2 hits
PS51408 TRANSFERRIN_LIKE_4, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRFL_MOUSE
AccessioniPrimary (citable) accession number: P08071
Secondary accession number(s): P70690
, Q61799, Q8CBA0, Q922P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 162 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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