UniProtKB - P08067 (UCRI_YEAST)
Cytochrome b-c1 complex subunit Rieske, mitochondrial
RIP1
Functioni
Miscellaneous
Catalytic activityi
- 2 [Fe(III)cytochrome c](out) + a quinol = 2 [Fe(II)cytochrome c](out) + a quinone + 2 H+(out)2 PublicationsEC:7.1.1.82 Publications
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 159 | Iron-sulfur (2Fe-2S)Combined sources4 Publications | 1 | |
Metal bindingi | 161 | Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources4 Publications | 1 | |
Metal bindingi | 178 | Iron-sulfur (2Fe-2S)Combined sources4 Publications | 1 | |
Metal bindingi | 181 | Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources4 Publications | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity Source: GO_Central
- ubiquinol-cytochrome-c reductase activity Source: SGD
GO - Biological processi
- aerobic respiration Source: SGD
- mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Keywordsi
Molecular function | Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Protein family/group databases
TCDBi | 3.D.3.3.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:7.1.1.82 Publications)Alternative name(s): Complex III subunit 5 Rieske iron-sulfur protein Short name: RISP Ubiquinol-cytochrome c oxidoreductase iron-sulfur subunit |
Gene namesi | Name:RIP1 Ordered Locus Names:YEL024W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YEL024W |
SGDi | S000000750, RIP1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Mitochondrion
- mitochondrial respiratory chain complex III Source: SGD
- mitochondrion Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 31 – 50 | Mitochondrial matrix2 PublicationsAdd BLAST | 20 | |
Transmembranei | 51 – 80 | Helical2 PublicationsAdd BLAST | 30 | |
Topological domaini | 81 – 215 | Mitochondrial intermembrane2 PublicationsAdd BLAST | 135 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 | G → D: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 159 | C → S: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 161 | H → R: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 163 | G → D: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 164 | C → S: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 166 | P → L: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 178 | C → S or Y: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 179 | P → L: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 180 | C → S: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 181 | H → R: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 183 | S → L: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 184 | H → R: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 186 | D → N: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 189 | G → D: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 195 | P → S: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 196 | A → T: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 203 | P → S: Loss of activity. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 22 | Mitochondrion2 PublicationsAdd BLAST | 22 | |
PropeptideiPRO_0000449195 | 23 – 30 | Removed in mature form2 Publications | 8 | |
ChainiPRO_0000030683 | 31 – 215 | Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST | 185 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 164 ↔ 180 | PROSITE-ProRule annotationCombined sources3 Publications |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | P08067 |
PaxDbi | P08067 |
PRIDEi | P08067 |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 10 subunits. The complex is composed of 3 respiratory subunits cytochrome b (COB), cytochrome c1 (CYT1) and Rieske protein (RIP1), 2 core protein subunits COR1 and QCR2, and 5 low-molecular weight protein subunits QCR6, QCR7, QCR8, QCR9 and QCR10 (PubMed:1657998, PubMed:2538628, PubMed:10873857, PubMed:11880631, PubMed:18390544, PubMed:30598554). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a monomer or a dimer of cytochrome c oxidase (complex IV, CIV), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554). RIP1 interacts with QCR10 on the intermembrane space (IMS) side, and with QCR9 (PubMed:30598556, PubMed:30598554).
9 PublicationsProtein-protein interaction databases
BioGRIDi | 36705, 252 interactors |
ComplexPortali | CPX-567, Mitochondrial electron transport complex III |
DIPi | DIP-6616N |
IntActi | P08067, 4 interactors |
STRINGi | 4932.YEL024W |
Miscellaneous databases
RNActi | P08067, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P08067 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08067 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 123 – 214 | RieskePROSITE-ProRule annotationAdd BLAST | 92 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 90 – 93 | Hinge1 Publication | 4 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
HOGENOMi | CLU_055690_0_1_1 |
InParanoidi | P08067 |
OMAi | VFHCPCH |
Family and domain databases
DisProti | DP00687 |
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD
60 70 80 90 100
KGRSYAYFMV GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA
110 120 130 140 150
IPLGKNVVVK WQGKPVFIRH RTPHEIQEAN SVDMSALKDP QTDADRVKDP
160 170 180 190 200
QWLIMLGICT HLGCVPIGEA GDFGGWFCPC HGSHYDISGR IRKGPAPLNL
210
EIPAYEFDGD KVIVG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23316 Genomic DNA Translation: AAA34980.1 M24500 Genomic DNA Translation: AAA34981.1 U18530 Genomic DNA Translation: AAB64501.1 AY558341 Genomic DNA Translation: AAS56667.1 BK006939 Genomic DNA Translation: DAA07628.1 |
PIRi | A29318 |
RefSeqi | NP_010890.3, NM_001178839.3 |
Genome annotation databases
EnsemblFungii | YEL024W_mRNA; YEL024W; YEL024W |
GeneIDi | 856689 |
KEGGi | sce:YEL024W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23316 Genomic DNA Translation: AAA34980.1 M24500 Genomic DNA Translation: AAA34981.1 U18530 Genomic DNA Translation: AAB64501.1 AY558341 Genomic DNA Translation: AAS56667.1 BK006939 Genomic DNA Translation: DAA07628.1 |
PIRi | A29318 |
RefSeqi | NP_010890.3, NM_001178839.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EZV | X-ray | 2.30 | E | 31-215 | [»] | |
1KB9 | X-ray | 2.30 | E | 31-215 | [»] | |
1KYO | X-ray | 2.97 | E/P | 31-215 | [»] | |
1P84 | X-ray | 2.50 | E | 31-215 | [»] | |
2IBZ | X-ray | 2.30 | E | 31-215 | [»] | |
3CX5 | X-ray | 1.90 | E/P | 31-215 | [»] | |
3CXH | X-ray | 2.50 | E/P | 31-215 | [»] | |
4PD4 | X-ray | 3.04 | E | 31-215 | [»] | |
6GIQ | electron microscopy | 3.23 | E/P | 1-215 | [»] | |
6HU9 | electron microscopy | 3.35 | E/P | 31-215 | [»] | |
6T0B | electron microscopy | 2.80 | E/P | 31-215 | [»] | |
6T15 | electron microscopy | 3.29 | E/P | 31-215 | [»] | |
6YMX | electron microscopy | 3.17 | E/P | 31-215 | [»] | |
SMRi | P08067 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36705, 252 interactors |
ComplexPortali | CPX-567, Mitochondrial electron transport complex III |
DIPi | DIP-6616N |
IntActi | P08067, 4 interactors |
STRINGi | 4932.YEL024W |
Protein family/group databases
TCDBi | 3.D.3.3.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Proteomic databases
MaxQBi | P08067 |
PaxDbi | P08067 |
PRIDEi | P08067 |
Protocols and materials databases
ABCDi | P08067, 1 sequenced antibody |
Genome annotation databases
EnsemblFungii | YEL024W_mRNA; YEL024W; YEL024W |
GeneIDi | 856689 |
KEGGi | sce:YEL024W |
Organism-specific databases
EuPathDBi | FungiDB:YEL024W |
SGDi | S000000750, RIP1 |
Phylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
HOGENOMi | CLU_055690_0_1_1 |
InParanoidi | P08067 |
OMAi | VFHCPCH |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P08067 |
PROi | PR:P08067 |
RNActi | P08067, protein |
Family and domain databases
DisProti | DP00687 |
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UCRI_YEAST | |
Accessioni | P08067Primary (citable) accession number: P08067 Secondary accession number(s): D3DLM4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD