UniProtKB - P08050 (CXA1_RAT)
Gap junction alpha-1 protein
Gja1
Functioni
GO - Molecular functioni
- alpha-tubulin binding Source: RGD
- beta-catenin binding Source: RGD
- beta-tubulin binding Source: RGD
- connexin binding Source: RGD
- disordered domain specific binding Source: CAFA
- efflux transmembrane transporter activity Source: RGD
- gap junction channel activity Source: CAFA
- gap junction channel activity involved in cell communication by electrical coupling Source: RGD
- gap junction hemi-channel activity Source: UniProtKB
- glutathione transmembrane transporter activity Source: RGD
- ion transmembrane transporter activity Source: RGD
- PDZ domain binding Source: CAFA
- protein domain specific binding Source: RGD
- protein tyrosine kinase binding Source: RGD
- scaffold protein binding Source: RGD
- SH3 domain binding Source: RGD
- signaling receptor binding Source: RGD
- tubulin binding Source: UniProtKB
GO - Biological processi
- adult heart development Source: RGD
- apoptotic process Source: RGD
- ATP transport Source: RGD
- atrial ventricular junction remodeling Source: RGD
- blood vessel morphogenesis Source: RGD
- bone development Source: UniProtKB
- bone remodeling Source: UniProtKB
- cardiac conduction Source: RGD
- cell-cell junction organization Source: RGD
- cell-cell signaling Source: RGD
- cell communication Source: RGD
- cell communication by chemical coupling Source: RGD
- cell communication by electrical coupling Source: RGD
- cellular protein localization Source: RGD
- cellular response to amyloid-beta Source: RGD
- cellular response to mechanical stimulus Source: RGD
- cellular response to parathyroid hormone stimulus Source: RGD
- cellular response to pH Source: RGD
- chronic inflammatory response Source: RGD
- decidualization Source: RGD
- embryonic digit morphogenesis Source: RGD
- embryonic heart tube development Source: RGD
- endothelium development Source: RGD
- epididymis development Source: RGD
- epithelial cell maturation Source: RGD
- establishment of mitotic spindle orientation Source: RGD
- export across plasma membrane Source: RGD
- gap junction assembly Source: UniProtKB
- glutamate secretion Source: RGD
- heart development Source: RGD
- heart looping Source: RGD
- in utero embryonic development Source: RGD
- ion transmembrane transport Source: RGD
- lens development in camera-type eye Source: RGD
- maintenance of protein localization in endoplasmic reticulum Source: RGD
- male gonad development Source: RGD
- microtubule-based transport Source: UniProtKB
- milk ejection reflex Source: RGD
- negative regulation of cardiac muscle cell proliferation Source: RGD
- negative regulation of cell growth Source: UniProtKB
- negative regulation of cell population proliferation Source: RGD
- negative regulation of DNA biosynthetic process Source: RGD
- negative regulation of endothelial cell proliferation Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of gonadotropin secretion Source: RGD
- negative regulation of trophoblast cell migration Source: RGD
- negative regulation of wound healing Source: RGD
- neuron migration Source: RGD
- neuron projection morphogenesis Source: RGD
- osteoblast differentiation Source: RGD
- positive regulation of behavioral fear response Source: RGD
- positive regulation of blood vessel diameter Source: RGD
- positive regulation of cell communication by chemical coupling Source: RGD
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of glomerular filtration Source: RGD
- positive regulation of insulin secretion Source: RGD
- positive regulation of mesodermal cell differentiation Source: RGD
- positive regulation of morphogenesis of an epithelium Source: RGD
- positive regulation of protein catabolic process Source: RGD
- positive regulation of stem cell proliferation Source: RGD
- positive regulation of striated muscle tissue development Source: RGD
- positive regulation of vascular associated smooth muscle cell proliferation Source: RGD
- positive regulation of vasoconstriction Source: RGD
- regulation of apoptotic process Source: UniProtKB
- regulation of atrial cardiac muscle cell membrane depolarization Source: RGD
- regulation of bicellular tight junction assembly Source: RGD
- regulation of bone mineralization Source: RGD
- regulation of bone remodeling Source: RGD
- regulation of calcium ion transport Source: RGD
- regulation of cell communication by electrical coupling Source: RGD
- regulation of heart contraction Source: RGD
- regulation of transmembrane transporter activity Source: CAFA
- regulation of ventricular cardiac muscle cell membrane depolarization Source: RGD
- regulation of ventricular cardiac muscle cell membrane repolarization Source: RGD
- response to estradiol Source: RGD
- response to fluid shear stress Source: RGD
- response to glucose Source: RGD
- response to ischemia Source: RGD
- response to lipopolysaccharide Source: RGD
- response to peptide hormone Source: RGD
- response to pH Source: RGD
- response to retinoic acid Source: RGD
- signal transduction Source: RGD
- skeletal muscle tissue regeneration Source: RGD
- spermatogenesis Source: UniProtKB
- T cell activation Source: RGD
- T cell proliferation Source: RGD
- transmembrane transport Source: CAFA
- vascular transport Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-190840, Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane R-RNO-190861, Gap junction assembly R-RNO-190873, Gap junction degradation R-RNO-191650, Regulation of gap junction activity R-RNO-196025, Formation of annular gap junctions |
Protein family/group databases
TCDBi | 1.A.24.1.1, the gap junction-forming connexin (connexin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction alpha-1 proteinAlternative name(s): Connexin-43 Short name: Cx43 Gap junction 43 kDa heart protein |
Gene namesi | Name:Gja1 Synonyms:Cxn-43 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2690, Gja1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- gap junction 3 Publications
Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.By similarity
Cytoskeleton
- intermediate filament Source: RGD
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Endosome
- early endosome Source: RGD
- endosome Source: RGD
- late endosome Source: RGD
- multivesicular body Source: RGD
Golgi apparatus
- Golgi apparatus Source: BHF-UCL
- Golgi membrane Source: Reactome
Lysosome
- lysosome Source: RGD
Mitochondrion
- mitochondrial outer membrane Source: RGD
- mitochondrion Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- connexin complex Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- lateral plasma membrane Source: RGD
- plasma membrane Source: BHF-UCL
Other locations
- cell junction Source: RGD
- cell-cell contact zone Source: RGD
- cell-cell junction Source: RGD
- contractile fiber Source: RGD
- cytoplasm Source: RGD
- fascia adherens Source: RGD
- gap junction Source: UniProtKB
- Golgi-associated vesicle membrane Source: Reactome
- intercalated disc Source: RGD
- membrane Source: MGI
- membrane raft Source: BHF-UCL
- protein-containing complex Source: CAFA
- tight junction Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 23 | Cytoplasmic2 PublicationsAdd BLAST | 22 | |
Transmembranei | 24 – 44 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 45 – 76 | Extracellular2 PublicationsAdd BLAST | 32 | |
Transmembranei | 77 – 97 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 98 – 155 | Cytoplasmic2 PublicationsAdd BLAST | 58 | |
Transmembranei | 156 – 176 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 177 – 207 | Extracellular2 PublicationsAdd BLAST | 31 | |
Transmembranei | 208 – 228 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 229 – 382 | Cytoplasmic2 PublicationsAdd BLAST | 154 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Endoplasmic reticulum, Gap junction, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 368 | S → A: Loss of phosphorylation by PKC/PRKCD. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000057804 | 2 – 382 | Gap junction alpha-1 proteinAdd BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 54 ↔ 192 | By similarity | ||
Cross-linki | 144 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Disulfide bondi | 187 ↔ 198 | By similarity | ||
Cross-linki | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 247 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 255 | PhosphoserineBy similarity | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 262 | PhosphoserineBy similarity | 1 | |
Modified residuei | 271 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 275 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 306 | PhosphoserineCombined sources | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 | |
Modified residuei | 325 | PhosphoserineCombined sources | 1 | |
Modified residuei | 326 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 328 | PhosphoserineCombined sources | 1 | |
Modified residuei | 330 | PhosphoserineCombined sources | 1 | |
Modified residuei | 365 | Phosphoserine1 Publication | 1 | |
Modified residuei | 368 | Phosphoserine; by PKC/PRKCG and PKC/PRKCD1 Publication1 Publication | 1 | |
Modified residuei | 369 | Phosphoserine1 Publication | 1 | |
Modified residuei | 373 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugationProteomic databases
PaxDbi | P08050 |
PRIDEi | P08050 |
PTM databases
iPTMneti | P08050 |
PhosphoSitePlusi | P08050 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000000805, Expressed in female gonad and 22 other tissues |
Genevisiblei | P08050, RN |
Interactioni
Subunit structurei
A connexon is composed of a hexamer of connexins (PubMed:1371548).
Interacts with SGSM3 (By similarity).
Interacts with RIC1/CIP150 (By similarity).
Interacts with CNST and CSNK1D (By similarity).
Interacts (via C-terminus) with TJP1 (PubMed:15492000, PubMed:18636092).
Interacts (via C-terminus) with SRC (via SH3 domain) (PubMed:15492000).
Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (PubMed:20127391).
Interacts with NOV (PubMed:15181016, PubMed:15213231).
Interacts with TMEM65 (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsP08050
With | #Exp. | IntAct |
---|---|---|
Kcnj8 [Q63664] | 4 | EBI-476947,EBI-6991142 |
Sgsm3 [Q8VCZ6] from Mus musculus. | 4 | EBI-476947,EBI-525155 |
UBQLN4 [Q9NRR5] from Homo sapiens. | 2 | EBI-476947,EBI-711226 |
GO - Molecular functioni
- alpha-tubulin binding Source: RGD
- beta-catenin binding Source: RGD
- beta-tubulin binding Source: RGD
- connexin binding Source: RGD
- disordered domain specific binding Source: CAFA
- PDZ domain binding Source: CAFA
- protein domain specific binding Source: RGD
- protein tyrosine kinase binding Source: RGD
- scaffold protein binding Source: RGD
- SH3 domain binding Source: RGD
- signaling receptor binding Source: RGD
- tubulin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 246562, 302 interactors |
CORUMi | P08050 |
DIPi | DIP-34793N |
IntActi | P08050, 18 interactors |
MINTi | P08050 |
STRINGi | 10116.ENSRNOP00000001054 |
Structurei
Secondary structure
3D structure databases
BMRBi | P08050 |
SMRi | P08050 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08050 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 244 – 382 | Interaction with NOV2 PublicationsAdd BLAST | 139 | |
Regioni | 264 – 382 | Interaction with UBQLN4By similarityAdd BLAST | 119 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QRAE, Eukaryota |
GeneTreei | ENSGT00990000203503 |
HOGENOMi | CLU_037388_0_0_1 |
InParanoidi | P08050 |
OMAi | LVIQWYM |
OrthoDBi | 823525at2759 |
PhylomeDBi | P08050 |
TreeFami | TF329606 |
Family and domain databases
DisProti | DP00278 |
Gene3Di | 1.20.1440.80, 1 hit 1.20.5.1130, 1 hit |
InterProi | View protein in InterPro IPR035091, Alpha_helix_dom_sf IPR000500, Connexin IPR002261, Connexin43 IPR013124, Connexin43_C IPR034634, Connexin_C IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit PTHR11984:SF33, PTHR11984:SF33, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit PF03508, Connexin43, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01132, CONNEXINA1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
SUPFAMi | SSF118220, SSF118220, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS
60 70 80 90 100
AFRCNTQQPG CENVCYDKSF PISHVRFWVL QIIFVSVPTL LYLAHVFYVM
110 120 130 140 150
RKEEKLNKKE EELKVAQTDG VNVEMHLKQI EIKKFKYGIE EHGKVKMRGG
160 170 180 190 200
LLRTYIISIL FKSVFEVAFL LIQWYIYGFS LSAVYTCKRD PCPHQVDCFL
210 220 230 240 250
SRPTEKTIFI IFMLVVSLVS LALNIIELFY VFFKGVKDRV KGRSDPYHAT
260 270 280 290 300
TGPLSPSKDC GSPKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN
310 320 330 340 350
YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNAKKVAAG
360 370 380
HELQPLAIVD QRPSSRASSR ASSRPRPDDL EI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | G → A AA sequence (PubMed:2987225).Curated | 1 | |
Sequence conflicti | 16 | A → T no nucleotide entry (PubMed:1852114).Curated | 1 | |
Sequence conflicti | 28 | V → I AA sequence (PubMed:2987225).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06656 mRNA Translation: CAA29855.1 AY324140 mRNA Translation: AAP88589.1 BC081842 mRNA Translation: AAH81842.1 |
PIRi | A24047 S00532 |
RefSeqi | NP_036699.1, NM_012567.2 |
Genome annotation databases
Ensembli | ENSRNOT00000001054; ENSRNOP00000001054; ENSRNOG00000000805 |
GeneIDi | 24392 |
KEGGi | rno:24392 |
UCSCi | RGD:2690, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06656 mRNA Translation: CAA29855.1 AY324140 mRNA Translation: AAP88589.1 BC081842 mRNA Translation: AAH81842.1 |
PIRi | A24047 S00532 |
RefSeqi | NP_036699.1, NM_012567.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R5S | NMR | - | A | 255-382 | [»] | |
2N8T | NMR | - | B | 276-289 | [»] | |
3CYY | X-ray | 2.40 | C/D | 374-382 | [»] | |
BMRBi | P08050 | |||||
SMRi | P08050 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246562, 302 interactors |
CORUMi | P08050 |
DIPi | DIP-34793N |
IntActi | P08050, 18 interactors |
MINTi | P08050 |
STRINGi | 10116.ENSRNOP00000001054 |
Protein family/group databases
TCDBi | 1.A.24.1.1, the gap junction-forming connexin (connexin) family |
PTM databases
iPTMneti | P08050 |
PhosphoSitePlusi | P08050 |
Proteomic databases
PaxDbi | P08050 |
PRIDEi | P08050 |
Genome annotation databases
Ensembli | ENSRNOT00000001054; ENSRNOP00000001054; ENSRNOG00000000805 |
GeneIDi | 24392 |
KEGGi | rno:24392 |
UCSCi | RGD:2690, rat |
Organism-specific databases
CTDi | 2697 |
RGDi | 2690, Gja1 |
Phylogenomic databases
eggNOGi | ENOG502QRAE, Eukaryota |
GeneTreei | ENSGT00990000203503 |
HOGENOMi | CLU_037388_0_0_1 |
InParanoidi | P08050 |
OMAi | LVIQWYM |
OrthoDBi | 823525at2759 |
PhylomeDBi | P08050 |
TreeFami | TF329606 |
Enzyme and pathway databases
Reactomei | R-RNO-190840, Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane R-RNO-190861, Gap junction assembly R-RNO-190873, Gap junction degradation R-RNO-191650, Regulation of gap junction activity R-RNO-196025, Formation of annular gap junctions |
Miscellaneous databases
EvolutionaryTracei | P08050 |
PROi | PR:P08050 |
Gene expression databases
Bgeei | ENSRNOG00000000805, Expressed in female gonad and 22 other tissues |
Genevisiblei | P08050, RN |
Family and domain databases
DisProti | DP00278 |
Gene3Di | 1.20.1440.80, 1 hit 1.20.5.1130, 1 hit |
InterProi | View protein in InterPro IPR035091, Alpha_helix_dom_sf IPR000500, Connexin IPR002261, Connexin43 IPR013124, Connexin43_C IPR034634, Connexin_C IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit PTHR11984:SF33, PTHR11984:SF33, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit PF03508, Connexin43, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01132, CONNEXINA1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
SUPFAMi | SSF118220, SSF118220, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXA1_RAT | |
Accessioni | P08050Primary (citable) accession number: P08050 Secondary accession number(s): Q53ZE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 208 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families