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Protein

Early growth response protein 1

Gene

Egr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator (PubMed:8336701, PubMed:8703054, PubMed:15958557). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (PubMed:8703054, PubMed:15958557, PubMed:2028256, PubMed:8939742). Binds double-stranded target DNA, irrespective of the cytosine methylation status (By similarity). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia (PubMed:11100120, PubMed:15958557). Plays a role in the regulation of cell survival, proliferation and cell death (PubMed:15265859, PubMed:15958557). Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation (PubMed:15958557). Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy (PubMed:15265859). Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia (PubMed:11100120). Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (PubMed:8703054).By similarityCurated7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri336 – 360C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri366 – 388C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri394 – 416C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
Nerve growth factor-induced protein A1 Publication
Short name:
NGFI-A1 Publication
Transcription factor Zif2681 Publication
Zinc finger protein Krox-241 Publication
Gene namesi
Name:Egr1
Synonyms:Egr-1, Krox-241 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:95295 Egr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice appear grossly normal, but females are anestrous and infertile with an uterus weight that is roughly 30% of that of wild-type. Ovaries contain normal numbers of follicles, but lack corpora lutea. Serum progesterone levels are strongly reduced; estradiol levels are normal. The level of luteinizing hormone (LHB) in the pituitary is strongly reduced in males and not detectable in females (PubMed:8703054). Responses to ischemia and hypoxia are blunted, leading to reduced tissue damage in response to ischemia and increased survival (PubMed:11100120). Liver regeneration is impaired after partial hepatectomy, due to impaired mitotic progress and reduced proliferation of hepatocytes (PubMed:15265859). Untreated mutant mice do not display an increased tendency to develop tumors, but develop tumors earlier than wild-type when treated first with a tumor initiator, and then with a tumor promoter (PubMed:15958557).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471101 – 533Early growth response protein 1Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP08046
PRIDEiP08046

PTM databases

iPTMnetiP08046
PhosphoSitePlusiP08046

Expressioni

Tissue specificityi

Detected in lung vasculature and in mononuclear phagocytes (PubMed:11100120). Detected in liver (at protein level) (PubMed:15265859). Detected in lung vasculature and in mononuclear phagocytes (PubMed:11100120).2 Publications

Inductioni

By growth factors (PubMed:3133658). Up-regulated in lung vasculature in response to reperfusion after ischemia (PubMed:11100120). Up-regulated in liver in response to partial hepatectomy (PubMed:15265859).3 Publications

Gene expression databases

BgeeiENSMUSG00000038418 Expressed in 340 organ(s), highest expression level in cumulus cell
CleanExiMM_EGR1
ExpressionAtlasiP08046 baseline and differential
GenevisibleiP08046 MM

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei334Interaction with DNACombined sources2 Publications1
Sitei345Interaction with DNACombined sources2 Publications1
Sitei349Interaction with DNACombined sources2 Publications1
Sitei355Interaction with DNACombined sources2 Publications1
Sitei373Interaction with DNACombined sources2 Publications1
Sitei377Interaction with DNACombined sources2 Publications1
Sitei401Interaction with DNACombined sources2 Publications1
Sitei405Interaction with DNACombined sources2 Publications1
Sitei411Interaction with DNACombined sources2 Publications1

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199404, 7 interactors
STRINGi10090.ENSMUSP00000069616

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08046
SMRiP08046
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08046

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi57 – 81Gly/Ser-richAdd BLAST25

Domaini

Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers (PubMed:1740423, PubMed:8336701, PubMed:2028256, PubMed:8939742). The first, most N-terminal zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif (PubMed:2028256, PubMed:8939742). Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine (By similarity).By similarity3 Publications

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri336 – 360C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri366 – 388C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri394 – 416C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00550000074455
HOGENOMiHOG000036856
HOVERGENiHBG003909
InParanoidiP08046
KOiK09203
OMAiFATQTGS
OrthoDBiEOG091G06VX
PhylomeDBiP08046
TreeFamiTF318980

Family and domain databases

InterProiView protein in InterPro
IPR021849 EGR
IPR021839 EGR1_C
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF11914 DUF3432, 1 hit
PF11928 DUF3446, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequencei

Sequence statusi: Complete.

P08046-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
60 70 80 90 100
AAGTPEGSGG NSSSSTSSGG GGGGGSNSGS SAFNPQGEPS EQPYEHLTTE
110 120 130 140 150
SFSDIALNNE KAMVETSYPS QTTRLPPITY TGRFSLEPAP NSGNTLWPEP
160 170 180 190 200
LFSLVSGLVS MTNPPTSSSS APSPAASSSS SASQSPPLSC AVPSNDSSPI
210 220 230 240 250
YSAAPTFPTP NTDIFPEPQS QAFPGSAGTA LQYPPPAYPA TKGGFQVPMI
260 270 280 290 300
PDYLFPQQQG DLSLGTPDQK PFQGLENRTQ QPSLTPLSTI KAFATQSGSQ
310 320 330 340 350
DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS
360 370 380 390 400
DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG
410 420 430 440 450
RKFARSDERK RHTKIHLRQK DKKADKSVVA SPAASSLSSY PSPVATSYPS
460 470 480 490 500
PATTSFPSPV PTSYSSPGSS TYPSPAHSGF PSPSVATTFA SVPPAFPTQV
510 520 530
SSFPSAGVSS SFSTSTGLSD MTATFSPRTI EIC
Length:533
Mass (Da):56,590
Last modified:November 1, 1990 - v2
Checksum:i36E3935767CEAA0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76S → T in AAB00468 (PubMed:2511075).Curated1
Sequence conflicti76S → T in AAA39382 (PubMed:3133658).Curated1
Sequence conflicti80S → T in AAB00468 (PubMed:2511075).Curated1
Sequence conflicti80S → T in AAA39382 (PubMed:3133658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA Translation: AAA37544.1
M22326 mRNA Translation: AAA40416.1
M28845, M28844 Genomic DNA Translation: AAB00468.1
M19643 mRNA Translation: AAA39382.1
CCDSiCCDS29136.1
PIRiJS0304
RefSeqiNP_031939.1, NM_007913.5
UniGeneiMm.181959

Genome annotation databases

EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418
ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418
GeneIDi13653
KEGGimmu:13653
UCSCiuc008elt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20157 mRNA Translation: AAA37544.1
M22326 mRNA Translation: AAA40416.1
M28845, M28844 Genomic DNA Translation: AAB00468.1
M19643 mRNA Translation: AAA39382.1
CCDSiCCDS29136.1
PIRiJS0304
RefSeqiNP_031939.1, NM_007913.5
UniGeneiMm.181959

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A1FX-ray2.10A333-421[»]
1A1GX-ray1.90A333-421[»]
1A1HX-ray1.60A333-421[»]
1A1IX-ray1.60A333-421[»]
1A1JX-ray2.00A333-421[»]
1A1KX-ray1.90A333-421[»]
1A1LX-ray2.30A333-421[»]
1AAYX-ray1.60A333-421[»]
1F2IX-ray2.35G/H/I/J/K/L334-389[»]
1G2DX-ray2.20C/F333-421[»]
1G2FX-ray2.00C/F333-421[»]
1JK1X-ray1.90A333-421[»]
1JK2X-ray1.65A333-421[»]
1LLMX-ray1.50C/D364-412[»]
1P47X-ray2.20A/B333-419[»]
1ZAAX-ray2.10C332-418[»]
ProteinModelPortaliP08046
SMRiP08046
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199404, 7 interactors
STRINGi10090.ENSMUSP00000069616

PTM databases

iPTMnetiP08046
PhosphoSitePlusiP08046

Proteomic databases

PaxDbiP08046
PRIDEiP08046

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064795; ENSMUSP00000069616; ENSMUSG00000038418
ENSMUST00000165033; ENSMUSP00000126931; ENSMUSG00000038418
GeneIDi13653
KEGGimmu:13653
UCSCiuc008elt.1 mouse

Organism-specific databases

CTDi1958
MGIiMGI:95295 Egr1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00550000074455
HOGENOMiHOG000036856
HOVERGENiHBG003909
InParanoidiP08046
KOiK09203
OMAiFATQTGS
OrthoDBiEOG091G06VX
PhylomeDBiP08046
TreeFamiTF318980

Miscellaneous databases

ChiTaRSiEgr1 mouse
EvolutionaryTraceiP08046
PROiPR:P08046
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038418 Expressed in 340 organ(s), highest expression level in cumulus cell
CleanExiMM_EGR1
ExpressionAtlasiP08046 baseline and differential
GenevisibleiP08046 MM

Family and domain databases

InterProiView protein in InterPro
IPR021849 EGR
IPR021839 EGR1_C
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF11914 DUF3432, 1 hit
PF11928 DUF3446, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEGR1_MOUSE
AccessioniPrimary (citable) accession number: P08046
Secondary accession number(s): Q61777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1990
Last modified: November 7, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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