UniProtKB - P08037 (B4GT1_BOVIN)
Protein
Beta-1,4-galactosyltransferase 1
Gene
B4GALT1
Organism
Bos taurus (Bovine)
Status
Functioni
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. The secreted form is responsible for the synthesis of complex-type to N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids (PubMed:9405390).1 Publication
Catalytic activityi
- EC:2.4.1.222 Publications
- an N-acetyl-β-D-glucosaminyl derivative + UDP-α-D-galactose = a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + H+ + UDP1 PublicationEC:2.4.1.381 PublicationThis reaction proceeds in the forward1 Publication direction.
- N-acetyl-D-glucosamine + UDP-α-D-galactose = β-D-galactosyl-(1→4)-N-acetyl-D-glucosamine + H+ + UDP1 PublicationEC:2.4.1.901 PublicationThis reaction proceeds in the forward1 Publication direction.
- a β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-β-D-Glc-(1↔1)-Cer(d18:1(4E)) + UDP-α-D-galactose = a neolactoside nLc4Cer(d18:1(4E)) + H+ + UDP1 PublicationEC:2.4.1.2751 PublicationThis reaction proceeds in the forward1 Publication direction.
- β-D-glucosylceramide + UDP-α-D-galactose = β-D-galactosyl-(1→4)-β-D-glucosyl-(1↔1)-ceramide + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a neolactoside IV3-β-GlcNAc-nLc4Cer + UDP-α-D-galactose = β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-β-D-glucosyl-(1↔1ʼ)-ceramide + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Mn2+By similarity
Kineticsi
- KM=0.034 mM for UDP-galactose1 Publication
- KM=0.53 mM for benzyl-beta-D-GlcNAc1 Publication
- KM=9.6 mM for D-GlcNAc1 Publication
- Vmax=73 pmol/min/mg enzyme towards UDP-galactose1 Publication
- Vmax=44.1 pmol/min/mg enzyme towards for benzyl-beta-D-GlcNAc1 Publication
- Vmax=56.8 pmol/min/mg enzyme towards D-GlcNAc1 Publication
- Vmax=297 nmol/h/mg enzyme towards lactotriaosylceramide (d18:1(4E))1 Publication
- Vmax=333 nmol/h/mg enzyme towards lactopentaosylceramide (d18:1(4E))1 Publication
- Vmax=40.3 nmol/h/mg enzyme towards GlcCer1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 254 | Manganese | 1 | |
Binding sitei | 314 | UDP-alpha-D-galactose | 1 | |
Metal bindingi | 347 | Manganese; via tele nitrogen | 1 | |
Binding sitei | 359 | N-acetyl-D-glucosamine | 1 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- lactose synthase activity Source: UniProtKB
- manganese ion binding Source: UniProtKB
- N-acetyllactosamine synthase activity Source: UniProtKB
- UDP-galactosyltransferase activity Source: UniProtKB
GO - Biological processi
- acute inflammatory response Source: Ensembl
- angiogenesis involved in wound healing Source: Ensembl
- binding of sperm to zona pellucida Source: Ensembl
- cell adhesion Source: Ensembl
- development of secondary sexual characteristics Source: Ensembl
- epithelial cell development Source: Ensembl
- extracellular matrix organization Source: Ensembl
- galactose metabolic process Source: Ensembl
- lactose biosynthetic process Source: CAFA
- leukocyte migration Source: Ensembl
- negative regulation of cell population proliferation Source: Ensembl
- oligosaccharide biosynthetic process Source: UniProtKB
- penetration of zona pellucida Source: Ensembl
- positive regulation of apoptotic process Source: Ensembl
- positive regulation of epithelial cell proliferation involved in wound healing Source: Ensembl
- protein galactosylation Source: UniProtKB
- protein N-linked glycosylation Source: CAFA
- regulation of acrosome reaction Source: Ensembl
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.133, 908 |
Reactomei | R-BTA-2022854, Keratan sulfate biosynthesis R-BTA-5653890, Lactose synthesis R-BTA-6798695, Neutrophil degranulation R-BTA-975577, N-Glycan antennae elongation |
SABIO-RKi | P08037 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
MoonProti | P08037 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1,4-galactosyltransferase 1Curated (EC:2.4.1.-1 Publication)Short name: Beta-1,4-GalTase 1 Short name: Beta4Gal-T1 Short name: b4Gal-T1 Alternative name(s): Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase1 Publication (EC:2.4.1.381 Publication) Lactose synthase A protein (EC:2.4.1.22) N-acetyllactosamine synthase1 Publication (EC:2.4.1.901 Publication) Nal synthase Neolactotriaosylceramide beta-1,4-galactosyltransferase1 Publication (EC:2.4.1.2751 Publication) UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 Cleaved into the following chain: |
Gene namesi | Name:B4GALT1 Synonyms:GALT, GGTB2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:26388, B4GALT1 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane By similarity; Single-pass type II membrane protein
Plasma membrane
Other locations
- Cell surface
- filopodium By similarity
Note: Found in trans cisternae of Golgi. B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity).By similarity
Golgi apparatus
Note: Found in trans cisternae of Golgi.
Extracellular region or secreted
- Secreted 1 Publication
Note: Soluble form found in body fluids.1 Publication
Extracellular region or secreted
- extracellular space Source: CAFA
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi trans cisterna Source: UniProtKB
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- brush border membrane Source: UniProtKB
- external side of plasma membrane Source: UniProtKB
Other locations
- desmosome Source: UniProtKB
- filopodium Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 44 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 45 – 402 | LumenalSequence analysisAdd BLAST | 358 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Golgi apparatus, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 314 | W → A: Reduces galactosyltransferase activity, lactose synthase activity and substrate binding by 99%. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3214 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000012276 | 1 – 402 | Beta-1,4-galactosyltransferase 1Add BLAST | 402 | |
ChainiPRO_0000296228 | ? – 402 | Processed beta-1,4-galactosyltransferase 1 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 90 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 117 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 134 ↔ 176 | |||
Disulfide bondi | 247 ↔ 266 |
Post-translational modificationi
The soluble form derives from the membrane forms by proteolytic processing.
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P08037 |
PeptideAtlasi | P08037 |
PRIDEi | P08037 |
PTM databases
iPTMneti | P08037 |
Expressioni
Tissue specificityi
Detected in milk (at protein level).1 Publication
Gene expression databases
Bgeei | ENSBTAG00000015249, Expressed in placenta and 19 other tissues |
Interactioni
Subunit structurei
Binary interactionsi
P08037
With | #Exp. | IntAct |
---|---|---|
Lalba [P29752] from Mus musculus. | 10 | EBI-1031436,EBI-1031454 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
IntActi | P08037, 1 interactor |
STRINGi | 9913.ENSBTAP00000020286 |
Chemistry databases
BindingDBi | P08037 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P08037 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P08037 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 187 – 191 | UDP-alpha-D-galactose binding | 5 | |
Regioni | 226 – 228 | UDP-alpha-D-galactose binding | 3 | |
Regioni | 253 – 254 | UDP-alpha-D-galactose binding | 2 | |
Regioni | 316 – 319 | N-acetyl-D-glucosamine binding | 4 | |
Regioni | 347 – 349 | UDP-alpha-D-galactose binding | 3 |
Sequence similaritiesi
Belongs to the glycosyltransferase 7 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3916, Eukaryota |
GeneTreei | ENSGT00940000155244 |
HOGENOMi | CLU_044391_0_1_1 |
InParanoidi | P08037 |
OMAi | HTKETMH |
OrthoDBi | 1201618at2759 |
TreeFami | TF312834 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR003859, Galactosyl_T IPR027791, Galactosyl_T_C IPR027995, Galactosyl_T_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR19300, PTHR19300, 1 hit |
Pfami | View protein in Pfam PF02709, Glyco_transf_7C, 1 hit PF13733, Glyco_transf_7N, 1 hit |
PRINTSi | PR02050, B14GALTRFASE |
SUPFAMi | SSF53448, SSF53448, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketIsoform Long (identifier: P08037-1) [UniParc]FASTAAdd to basket
Also known as: Cell surface
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKFREPLLGG SAAMPGASLQ RACRLLVAVC ALHLGVTLVY YLAGRDLRRL
60 70 80 90 100
PQLVGVHPPL QGSSHGAAAI GQPSGELRLR GVAPPPPLQN SSKPRSRAPS
110 120 130 140 150
NLDAYSHPGP GPGPGSNLTS APVPSTTTRS LTACPEESPL LVGPMLIEFN
160 170 180 190 200
IPVDLKLVEQ QNPKVKLGGR YTPMDCISPH KVAIIIPFRN RQEHLKYWLY
210 220 230 240 250
YLHPILQRQQ LDYGIYVINQ AGESMFNRAK LLNVGFKEAL KDYDYNCFVF
260 270 280 290 300
SDVDLIPMND HNTYRCFSQP RHISVAMDKF GFSLPYVQYF GGVSALSKQQ
310 320 330 340 350
FLSINGFPNN YWGWGGEDDD IYNRLAFRGM SVSRPNAVIG KCRMIRHSRD
360 370 380 390 400
KKNEPNPQRF DRIAHTKETM LSDGLNSLTY MVLEVQRYPL YTKITVDIGT
PS
Isoform Short (identifier: P08037-2) [UniParc]FASTAAdd to basket
Also known as: Golgi complex
The sequence of this isoform differs from the canonical sequence as follows:
1-13: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 65 – 66 | HG → QA (PubMed:2105947).Curated | 2 | |
Sequence conflicti | 158 | V → I in CAA32695 (PubMed:2502398).Curated | 1 | |
Sequence conflicti | 187 | P → L in CAA32695 (PubMed:2502398).Curated | 1 | |
Sequence conflicti | 205 – 206 | IL → MV in CAA32695 (PubMed:2502398).Curated | 2 | |
Sequence conflicti | 282 | F → L in AAA30534 (PubMed:2419911).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018801 | 1 – 13 | Missing in isoform Short. CuratedAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14558 mRNA Translation: CAA32695.1 BC120415 mRNA Translation: AAI20416.1 M13214 mRNA Translation: AAA30534.1 AF515786 mRNA Translation: AAM54035.2 M25398 Genomic DNA Translation: AAA30533.1 J05217 mRNA Translation: AAA30559.1 |
PIRi | I45897 S05018 |
RefSeqi | NP_803478.1, NM_177512.2 |
Genome annotation databases
Ensembli | ENSBTAT00000020286; ENSBTAP00000020286; ENSBTAG00000015249 [P08037-1] |
GeneIDi | 281781 |
KEGGi | bta:281781 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14558 mRNA Translation: CAA32695.1 BC120415 mRNA Translation: AAI20416.1 M13214 mRNA Translation: AAA30534.1 AF515786 mRNA Translation: AAM54035.2 M25398 Genomic DNA Translation: AAA30533.1 J05217 mRNA Translation: AAA30559.1 |
PIRi | I45897 S05018 |
RefSeqi | NP_803478.1, NM_177512.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FGX | X-ray | 2.40 | A/B | 115-402 | [»] | |
1FR8 | X-ray | 2.40 | A/B | 115-402 | [»] | |
1NF5 | X-ray | 2.00 | B/D | 130-402 | [»] | |
1NHE | X-ray | 2.50 | B/D | 130-402 | [»] | |
1NKH | X-ray | 2.00 | B/D | 130-402 | [»] | |
1NMM | X-ray | 2.00 | B/D | 130-402 | [»] | |
1NQI | X-ray | 2.00 | B/D | 130-402 | [»] | |
1NWG | X-ray | 2.32 | B/D | 130-402 | [»] | |
1O0R | X-ray | 2.30 | A/B | 130-402 | [»] | |
1O23 | X-ray | 2.32 | B/D | 130-402 | [»] | |
1OQM | X-ray | 2.10 | B/D | 130-402 | [»] | |
1PZT | X-ray | 1.92 | A | 130-402 | [»] | |
1PZY | X-ray | 2.30 | B/D | 130-402 | [»] | |
1TVY | X-ray | 2.30 | A/B | 130-402 | [»] | |
1TW1 | X-ray | 2.30 | A/B | 130-402 | [»] | |
1TW5 | X-ray | 2.30 | A/B | 130-402 | [»] | |
1YRO | X-ray | 1.90 | B/D | 130-402 | [»] | |
2FYC | X-ray | 2.00 | B/D | 130-402 | [»] | |
2FYD | X-ray | 2.00 | B/D | 130-400 | [»] | |
4KRV | X-ray | 2.40 | A/B | 130-402 | [»] | |
SMRi | P08037 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P08037, 1 interactor |
STRINGi | 9913.ENSBTAP00000020286 |
Chemistry databases
BindingDBi | P08037 |
ChEMBLi | CHEMBL3214 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
MoonProti | P08037 |
PTM databases
iPTMneti | P08037 |
Proteomic databases
PaxDbi | P08037 |
PeptideAtlasi | P08037 |
PRIDEi | P08037 |
Genome annotation databases
Ensembli | ENSBTAT00000020286; ENSBTAP00000020286; ENSBTAG00000015249 [P08037-1] |
GeneIDi | 281781 |
KEGGi | bta:281781 |
Organism-specific databases
CTDi | 2683 |
VGNCi | VGNC:26388, B4GALT1 |
Phylogenomic databases
eggNOGi | KOG3916, Eukaryota |
GeneTreei | ENSGT00940000155244 |
HOGENOMi | CLU_044391_0_1_1 |
InParanoidi | P08037 |
OMAi | HTKETMH |
OrthoDBi | 1201618at2759 |
TreeFami | TF312834 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.133, 908 |
Reactomei | R-BTA-2022854, Keratan sulfate biosynthesis R-BTA-5653890, Lactose synthesis R-BTA-6798695, Neutrophil degranulation R-BTA-975577, N-Glycan antennae elongation |
SABIO-RKi | P08037 |
Miscellaneous databases
EvolutionaryTracei | P08037 |
Gene expression databases
Bgeei | ENSBTAG00000015249, Expressed in placenta and 19 other tissues |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR003859, Galactosyl_T IPR027791, Galactosyl_T_C IPR027995, Galactosyl_T_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR19300, PTHR19300, 1 hit |
Pfami | View protein in Pfam PF02709, Glyco_transf_7C, 1 hit PF13733, Glyco_transf_7N, 1 hit |
PRINTSi | PR02050, B14GALTRFASE |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B4GT1_BOVIN | |
Accessioni | P08037Primary (citable) accession number: P08037 Secondary accession number(s): Q0VC05, Q8MIG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | October 17, 2006 | |
Last modified: | April 7, 2021 | |
This is version 203 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families