UniProtKB - P08033 (CXB1_RAT)
Protein
Gap junction beta-1 protein
Gene
Gjb1
Organism
Rattus norvegicus (Rat)
Status
Functioni
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
GO - Molecular functioni
- gap junction channel activity Source: RGD
- identical protein binding Source: RGD
GO - Biological processi
- cell-cell signaling Source: GO_Central
- cell communication Source: RGD
- epididymis development Source: RGD
- purine ribonucleotide transport Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-190704, Oligomerization of connexins into connexons |
Protein family/group databases
TCDBi | 1.A.24.1.2, the gap junction-forming connexin (connexin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction beta-1 proteinAlternative name(s): Connexin-32 Short name: Cx32 GAP junction 28 kDa liver protein |
Gene namesi | Name:Gjb1 Synonyms:Cxn-32 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61926, Gjb1 |
Subcellular locationi
Plasma membrane
Other locations
Plasma Membrane
- connexin complex Source: GO_Central
- lateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: RGD
- gap junction Source: RGD
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 23 – 45 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 46 – 75 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 76 – 95 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 96 – 130 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 131 – 153 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 154 – 191 | ExtracellularSequence analysisAdd BLAST | 38 | |
Transmembranei | 192 – 214 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 215 – 283 | CytoplasmicSequence analysisAdd BLAST | 69 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Gap junction, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057852 | 1 – 283 | Gap junction beta-1 proteinAdd BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 233 | PhosphoserineCombined sources | 1 | |
Modified residuei | 258 | PhosphoserineCombined sources | 1 | |
Modified residuei | 266 | PhosphoserineCombined sources | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P08033 |
PRIDEi | P08033 |
PTM databases
iPTMneti | P08033 |
PhosphoSitePlusi | P08033 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000003746, Expressed in liver and 21 other tissues |
ExpressionAtlasi | P08033, baseline and differential |
Genevisiblei | P08033, RN |
Interactioni
Subunit structurei
A connexon is composed of a hexamer of connexins.
Interacts with CNST (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248217, 1 interactor |
IntActi | P08033, 2 interactors |
STRINGi | 10116.ENSRNOP00000068248 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R1QN, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P08033 |
OMAi | YLVVRAC |
OrthoDBi | 1131301at2759 |
PhylomeDBi | P08033 |
TreeFami | TF329606 |
Family and domain databases
DisProti | DP01176 |
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002267, Connexin32 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01138, CONNEXINB1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P08033-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
60 70 80 90 100
FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
110 120 130 140 150
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
160 170 180 190 200
YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI
210 220 230 240 250
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
260 270 280
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A096MKE6 | A0A096MKE6_RAT | Gap junction beta-1 protein | Gjb1 | 95 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04070 mRNA Translation: CAA27705.1 X04303 mRNA Translation: CAA27846.1 M23565 Genomic DNA Translation: AAA81569.1 BC078868 mRNA Translation: AAH78868.1 AH003192 Genomic DNA Translation: AAA75195.1 |
PIRi | A92745, GJRT I52552 |
RefSeqi | NP_058947.1, NM_017251.2 XP_008771477.1, XM_008773255.2 XP_017457434.1, XM_017601945.1 |
Genome annotation databases
Ensembli | ENSRNOT00000076816; ENSRNOP00000068248; ENSRNOG00000003746 |
GeneIDi | 29584 |
KEGGi | rno:29584 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04070 mRNA Translation: CAA27705.1 X04303 mRNA Translation: CAA27846.1 M23565 Genomic DNA Translation: AAA81569.1 BC078868 mRNA Translation: AAH78868.1 AH003192 Genomic DNA Translation: AAA75195.1 |
PIRi | A92745, GJRT I52552 |
RefSeqi | NP_058947.1, NM_017251.2 XP_008771477.1, XM_008773255.2 XP_017457434.1, XM_017601945.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 248217, 1 interactor |
IntActi | P08033, 2 interactors |
STRINGi | 10116.ENSRNOP00000068248 |
Protein family/group databases
TCDBi | 1.A.24.1.2, the gap junction-forming connexin (connexin) family |
PTM databases
iPTMneti | P08033 |
PhosphoSitePlusi | P08033 |
Proteomic databases
PaxDbi | P08033 |
PRIDEi | P08033 |
Genome annotation databases
Ensembli | ENSRNOT00000076816; ENSRNOP00000068248; ENSRNOG00000003746 |
GeneIDi | 29584 |
KEGGi | rno:29584 |
Organism-specific databases
CTDi | 2705 |
RGDi | 61926, Gjb1 |
Phylogenomic databases
eggNOGi | ENOG502R1QN, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P08033 |
OMAi | YLVVRAC |
OrthoDBi | 1131301at2759 |
PhylomeDBi | P08033 |
TreeFami | TF329606 |
Enzyme and pathway databases
Reactomei | R-RNO-190704, Oligomerization of connexins into connexons |
Miscellaneous databases
PROi | PR:P08033 |
Gene expression databases
Bgeei | ENSRNOG00000003746, Expressed in liver and 21 other tissues |
ExpressionAtlasi | P08033, baseline and differential |
Genevisiblei | P08033, RN |
Family and domain databases
DisProti | DP01176 |
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002267, Connexin32 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01138, CONNEXINB1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXB1_RAT | |
Accessioni | P08033Primary (citable) accession number: P08033 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families