UniProtKB - P08028 (NCAP_INCCA)
Nucleoprotein
NP
Functioni
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
UniRule annotationGO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
- structural molecule activity Source: UniProtKB-UniRule
GO - Biological processi
- viral entry into host cell Source: UniProtKB-KW
- viral penetration into host nucleus Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NPUniRule annotation |
Organismi | Influenza C virus (strain C/California/1978) |
Taxonomic identifieri | 203224 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Gammainfluenzavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Subcellular locationi
- Virion UniRule annotation
- Host nucleus UniRule annotation
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, Host nucleus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079145 | 1 – 565 | NucleoproteinAdd BLAST | 565 |
Post-translational modificationi
Interactioni
Subunit structurei
Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 518 – 565 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 525 – 545 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 551 – 565 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSDRRQNRKT PDEQRKANAL IINENIEAYI AICKEVGLNG DEMLILENGI
60 70 80 90 100
AIEKAIRICC DGKYQEKREK KAREAQRADS NFNADSIGIR LVKRAGSGTN
110 120 130 140 150
ITYHAVVELT SRSRIVQILK SHWGNELNRA KIAGKRLGFS ALFASNLEAI
160 170 180 190 200
IYQRGRNAAR RNGSAELFTL TQGAGIETRY KWIMEKHIGI GVLIADAKGL
210 220 230 240 250
INGKREGKRG VDANVKLRAG TTGSPLERAM QGIEKKAFPG PLRALARRVV
260 270 280 290 300
KANYNDAREA LNVIAEASLL LKPQITNKMT MPWCMWLAAR LTLKDEFTNF
310 320 330 340 350
CAYAGRRAFE VFNIAMEKIG ICSFQGTIMN DDEIESIEDK AQVLMMACFG
360 370 380 390 400
LAYEDFSLVS AMVSHPLKLR NRMKIGNFRV GEKVSTVLSP LLRFTRWAAF
410 420 430 440 450
AQRFALQANT SREGTQISNS AVFAVERKIT TDVQRVEELL NKVQAHEDEP
460 470 480 490 500
LQTLYKKVRE QISIIGRNKS EIKEFLGSSM YDLNDQEKQN PINFRSGAHP
510 520 530 540 550
FFFEFDPDYN PIRVKRPKKP IAKRNSNISR LEEEGMDENS EIGQAKKMKP
560
LDQLASTSSN IPGEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17700 Genomic RNA Translation: AAA43798.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17700 Genomic RNA Translation: AAA43798.1 |
3D structure databases
SMRi | P08028 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_INCCA | |
Accessioni | P08028Primary (citable) accession number: P08028 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | June 2, 2021 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |