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Protein

Insulin-like growth factor I

Gene

Igf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity).By similarity

GO - Molecular functioni

  • growth factor activity Source: RGD
  • hormone activity Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • protein serine/threonine kinase activator activity Source: BHF-UCL
  • steroid binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionGrowth factor

Enzyme and pathway databases

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-RNO-2428928 IRS-related events triggered by IGF1R
R-RNO-2428933 SHC-related events triggered by IGF1R
R-RNO-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-RNO-422085 Synthesis, secretion, and deacylation of Ghrelin

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor I
Short name:
IGF-I
Alternative name(s):
Somatomedin
Gene namesi
Name:Igf1
Synonyms:Igf-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2868 Igf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000015681? – 481 Publication
Signal peptidei1 – ?Sequence analysis
ChainiPRO_000001568249 – 118Insulin-like growth factor IAdd BLAST70
PropeptideiPRO_0000015683119 – 153E peptideAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 96By similarity
Disulfide bondi66 ↔ 109By similarity
Disulfide bondi95 ↔ 100By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP08025
PRIDEiP08025

PTM databases

iPTMnetiP08025

Expressioni

Gene expression databases

BgeeiENSRNOG00000004517 Expressed in 10 organ(s), highest expression level in liver
ExpressionAtlasiP08025 baseline and differential
GenevisibleiP08025 RN

Interactioni

Subunit structurei

Forms a ternary complex with IGFR1 and ITGAV:ITGB3. Forms a ternary complex with IGFR1 and ITGA6:ITGB4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005995

Structurei

3D structure databases

ProteinModelPortaliP08025
SMRiP08025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 77BAdd BLAST29
Regioni78 – 89CAdd BLAST12
Regioni90 – 110AAdd BLAST21
Regioni111 – 118D8

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJ2T Eukaryota
ENOG4111MIV LUCA
GeneTreeiENSGT00530000063856
HOGENOMiHOG000233362
HOVERGENiHBG006137
InParanoidiP08025
KOiK05459
OMAiCFQSCDL
PhylomeDBiP08025
TreeFamiTF332820

Family and domain databases

InterProiView protein in InterPro
IPR022341 IGF-I
IPR016179 Insulin-like
IPR022350 Insulin-like_growth_factor
IPR036438 Insulin-like_sf
IPR022353 Insulin_CS
IPR022352 Insulin_family
PfamiView protein in Pfam
PF00049 Insulin, 2 hits
PRINTSiPR02002 INSLNLIKEGF
PR02005 INSLNLIKEGF1
PR00276 INSULINFAMLY
SMARTiView protein in SMART
SM00078 IlGF, 1 hit
SUPFAMiSSF56994 SSF56994, 1 hit
PROSITEiView protein in PROSITE
PS00262 INSULIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform IGF-IA (identifier: P08025-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSATAGP
60 70 80 90 100
ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC
110 120 130 140 150
DLRRLEMYCA PLKPTKSARS IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT

YRM
Length:153
Mass (Da):17,079
Last modified:February 1, 1991 - v3
Checksum:i966F3C0FA4EB3DE7
GO
Isoform IGF-IB (identifier: P08025-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-153: EVHLKNTSRGSAGNKTYRM → SQPLSTHKKRKLQRRRKGSTLEEH

Show »
Length:158
Mass (Da):17,831
Checksum:i27ECE1968BA152D3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WFZ5F8WFZ5_RAT
Insulin-like growth factor 1, isofo...
Igf1 rCG_48929
159Annotation score:
A0A0G2JX40A0A0G2JX40_RAT
Insulin-like growth factor I
Igf1
143Annotation score:

Sequence cautioni

The sequence AAA41385 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA41386 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA41387 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 112APL → VRC in AAA41385 (PubMed:3453891).Curated3
Sequence conflicti110 – 112APL → VRC in AAA41387 (PubMed:3453891).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012166135 – 153EVHLK…KTYRM → SQPLSTHKKRKLQRRRKGST LEEH in isoform IGF-IB. CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15651
, M15647, M15648, M15649 Genomic DNA Translation: AAA41215.1
M15650
, M15647, M15648, M15649 Genomic DNA Translation: AAA41214.1
M17335 mRNA Translation: AAA41386.1 Different initiation.
M15480 mRNA Translation: AAA41385.1 Different initiation.
M15481 mRNA Translation: AAA41387.1 Different initiation.
X06107 mRNA Translation: CAA29480.1
X06043 mRNA Translation: CAA29436.1
PIRiA27804
A40912
B27804
RefSeqiNP_001075946.2, NM_001082477.2
NP_001075947.1, NM_001082478.1
NP_001075948.1, NM_001082479.1 [P08025-1]
XP_006241253.1, XM_006241191.2
XP_006241254.1, XM_006241192.1
XP_006241255.1, XM_006241193.3 [P08025-1]
UniGeneiRn.201887
Rn.228346
Rn.6282

Genome annotation databases

EnsembliENSRNOT00000038780; ENSRNOP00000035899; ENSRNOG00000004517 [P08025-1]
GeneIDi24482
KEGGirno:24482

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15651
, M15647, M15648, M15649 Genomic DNA Translation: AAA41215.1
M15650
, M15647, M15648, M15649 Genomic DNA Translation: AAA41214.1
M17335 mRNA Translation: AAA41386.1 Different initiation.
M15480 mRNA Translation: AAA41385.1 Different initiation.
M15481 mRNA Translation: AAA41387.1 Different initiation.
X06107 mRNA Translation: CAA29480.1
X06043 mRNA Translation: CAA29436.1
PIRiA27804
A40912
B27804
RefSeqiNP_001075946.2, NM_001082477.2
NP_001075947.1, NM_001082478.1
NP_001075948.1, NM_001082479.1 [P08025-1]
XP_006241253.1, XM_006241191.2
XP_006241254.1, XM_006241192.1
XP_006241255.1, XM_006241193.3 [P08025-1]
UniGeneiRn.201887
Rn.228346
Rn.6282

3D structure databases

ProteinModelPortaliP08025
SMRiP08025
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005995

PTM databases

iPTMnetiP08025

Proteomic databases

PaxDbiP08025
PRIDEiP08025

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000038780; ENSRNOP00000035899; ENSRNOG00000004517 [P08025-1]
GeneIDi24482
KEGGirno:24482

Organism-specific databases

CTDi3479
RGDi2868 Igf1

Phylogenomic databases

eggNOGiENOG410IJ2T Eukaryota
ENOG4111MIV LUCA
GeneTreeiENSGT00530000063856
HOGENOMiHOG000233362
HOVERGENiHBG006137
InParanoidiP08025
KOiK05459
OMAiCFQSCDL
PhylomeDBiP08025
TreeFamiTF332820

Enzyme and pathway databases

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-RNO-2428928 IRS-related events triggered by IGF1R
R-RNO-2428933 SHC-related events triggered by IGF1R
R-RNO-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-RNO-422085 Synthesis, secretion, and deacylation of Ghrelin

Miscellaneous databases

PROiPR:P08025

Gene expression databases

BgeeiENSRNOG00000004517 Expressed in 10 organ(s), highest expression level in liver
ExpressionAtlasiP08025 baseline and differential
GenevisibleiP08025 RN

Family and domain databases

InterProiView protein in InterPro
IPR022341 IGF-I
IPR016179 Insulin-like
IPR022350 Insulin-like_growth_factor
IPR036438 Insulin-like_sf
IPR022353 Insulin_CS
IPR022352 Insulin_family
PfamiView protein in Pfam
PF00049 Insulin, 2 hits
PRINTSiPR02002 INSLNLIKEGF
PR02005 INSLNLIKEGF1
PR00276 INSULINFAMLY
SMARTiView protein in SMART
SM00078 IlGF, 1 hit
SUPFAMiSSF56994 SSF56994, 1 hit
PROSITEiView protein in PROSITE
PS00262 INSULIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIGF1_RAT
AccessioniPrimary (citable) accession number: P08025
Secondary accession number(s): P08024
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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