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Protein

Glucoamylase, intracellular sporulation-specific

Gene

SGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei198SubstrateBy similarity1
Active sitei261Proton acceptorPROSITE-ProRule annotation1
Active sitei264Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

  • glucan 1,4-alpha-glucosidase activity Source: SGD

GO - Biological processi

  • glycogen catabolic process Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:YIL099W-MONOMER
YEAST:YIL099W-MONOMER

Protein family/group databases

CAZyiGH15 Glycoside Hydrolase Family 15

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase, intracellular sporulation-specific (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:SGA1
Synonyms:SGA
Ordered Locus Names:YIL099W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL099W
SGDiS000001361 SGA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075248

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861201 – 549Glucoamylase, intracellular sporulation-specificAdd BLAST549

Proteomic databases

PaxDbiP08019
PRIDEiP08019

Interactioni

Protein-protein interaction databases

BioGridi34892, 27 interactors
IntActiP08019, 2 interactors
MINTiP08019
STRINGi4932.YIL099W

Chemistry databases

BindingDBiP08019

Structurei

3D structure databases

ProteinModelPortaliP08019
SMRiP08019
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Phylogenomic databases

HOGENOMiHOG000182646
InParanoidiP08019
KOiK01178
OMAiEGNPWFI
OrthoDBiEOG092C1NOF

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR011613 GH15-like
IPR000165 Glucoamylase
PfamiView protein in Pfam
PF00723 Glyco_hydro_15, 1 hit
PRINTSiPR00736 GLHYDRLASE15
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS00820 GLUCOAMYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P08019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARQKMFYNK LLGMLSVGFG FAWALENITI YEFDFGKGIL DQSYGGVFSN
60 70 80 90 100
NGPSQVQLRD AVLMNGTVVY DSNGAWDSSA LEEWLQGQKK VSIEKIFENI
110 120 130 140 150
GPSAVYPSIS PGVVIASPSQ THPDYFYQWI RDSALTINSI VSHSAGPAIE
160 170 180 190 200
TLLQYLNVSF HLQRSNNTLG AGIGYTNDTV ALGDPKWNVD NTAFTEDWGR
210 220 230 240 250
PQNDGPALRS IAILKIIDYI KQSGTDLGAK YPFQSTADIF DDIVRWDLRF
260 270 280 290 300
IIDHWNSSGF DLWEEVNGMH FFTLLVQLSA VDKSLSYFNA SERSSPFVEE
310 320 330 340 350
LRQTRRDISK FLVDPANGFI NGKYNYIVGT PMIADTLRSG LDISTLLAAN
360 370 380 390 400
TVHDAPSASH LPFDINDPAV LNTLHHLMLH MRSIYPINDS SKNATGIALG
410 420 430 440 450
RYPEDVYDGY GFGEGNPWVL ATCTASTTLY QLIYRHISEQ HDLVVPMNND
460 470 480 490 500
CSNAFWSELV FSNLTTLGND EGYLILEFNT PAFNQTIQKI FQLADSFLVK
510 520 530 540
LKAHVGTDGE LSEQFNKYTG FMQGAQHLTW SYTSFWDAYQ IRQEVLQSL
Length:549
Mass (Da):61,463
Last modified:February 1, 1995 - v2
Checksum:i6351E84F2CF4AB77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184D → H in CAA32071 (PubMed:3141213).Curated1
Sequence conflicti504 – 549HVGTD…VLQSL → TWEQTGN in AAA35042 (PubMed:3106330).CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA Translation: CAA86282.1
M16166 Genomic DNA Translation: AAA35042.1
X13858 Genomic DNA Translation: CAA32071.1
BK006942 Genomic DNA Translation: DAA08454.1
PIRiS48474
RefSeqiNP_012167.3, NM_001179447.3

Genome annotation databases

EnsemblFungiiYIL099W; YIL099W; YIL099W
GeneIDi854708
KEGGisce:YIL099W

Similar proteinsi

Entry informationi

Entry nameiAMYG_YEAST
AccessioniPrimary (citable) accession number: P08019
Secondary accession number(s): D6VVI8, Q07070
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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