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Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Activity regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide, except in the testis. Activation also occurs via nitration of Tyr-93 by peroxynitrite.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Glutathione1 Publication1
Sitei50Activates the enzyme when modified1
Binding sitei73Glutathione1 Publication1
Binding sitei74Glutathione1 Publication1
Binding sitei76Glutathione1 Publication1
Binding sitei81Glutathione1 Publication1
Binding sitei121Glutathione1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 5301
ReactomeiR-RNO-156590 Glutathione conjugation
R-RNO-5423646 Aflatoxin activation and detoxification
R-RNO-6798695 Neutrophil degranulation
SABIO-RKiP08011

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:Mgst1
Synonyms:Gst12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi70927 Mgst1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9Lumenal7
Transmembranei10 – 33HelicalAdd BLAST24
Topological domaini34 – 62CytoplasmicAdd BLAST29
Transmembranei63 – 96HelicalAdd BLAST34
Topological domaini97 – 99Lumenal3
Transmembranei100 – 123HelicalAdd BLAST24
Topological domaini124 – 128Cytoplasmic5
Transmembranei129 – 148HelicalAdd BLAST20
Topological domaini149 – 155Lumenal7

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76H → Q: Decreased enzyme activity. 1 Publication1
Mutagenesisi81E → Q: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002177392 – 155Microsomal glutathione S-transferase 1Add BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei55N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei93Nitrated tyrosine1 Publication1

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.1 Publication

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

PaxDbiP08011
PRIDEiP08011

PTM databases

iPTMnetiP08011
PhosphoSitePlusiP08011

Expressioni

Tissue specificityi

Highest in the liver, followed by kidney and testis and much lower in seminal vesicles, spleen, lung and brain.

Gene expression databases

BgeeiENSRNOG00000007743 Expressed in 9 organ(s), highest expression level in liver
ExpressionAtlasiP08011 baseline and differential
GenevisibleiP08011 RN

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010579

Structurei

Secondary structure

1155
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08011
SMRiP08011
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08011

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1 Eukaryota
ENOG4111VJG LUCA
GeneTreeiENSGT00390000011980
HOGENOMiHOG000231759
HOVERGENiHBG052470
InParanoidiP08011
KOiK00799
OMAiFYRMTRK
OrthoDBiEOG091G14I4
PhylomeDBiP08011
TreeFamiTF105327

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
SUPFAMiSSF161084 SSF161084, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08011-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADLKQLMDN EVLMAFTSYA TIILAKMMFL SSATAFQRLT NKVFANPEDC
60 70 80 90 100
AGFGKGENAK KFLRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STALIHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR

SRLYL
Length:155
Mass (Da):17,472
Last modified:January 23, 2007 - v3
Checksum:i8FB62EEDF5A74489
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27M → V AA sequence (PubMed:3932348).Curated1
Sequence conflicti55K → G AA sequence (PubMed:3932348).Curated1
Sequence conflicti68K → T AA sequence (PubMed:3932348).Curated1
Sequence conflicti97G → A AA sequence (PubMed:3932348).Curated1
Sequence conflicti101S → P AA sequence (PubMed:3932348).Curated1
Sequence conflicti134F → K AA sequence (PubMed:3932348).Curated1
Sequence conflicti141T → D AA sequence (PubMed:3932348).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03752 mRNA Translation: AAA41281.1
BC063150 mRNA Translation: AAH63150.1
PIRiA28083
RefSeqiNP_599176.1, NM_134349.3
UniGeneiRn.2580

Genome annotation databases

EnsembliENSRNOT00000010579; ENSRNOP00000010579; ENSRNOG00000007743
GeneIDi171341
KEGGirno:171341
UCSCiRGD:70927 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03752 mRNA Translation: AAA41281.1
BC063150 mRNA Translation: AAH63150.1
PIRiA28083
RefSeqiNP_599176.1, NM_134349.3
UniGeneiRn.2580

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H8Aelectron microscopy3.20A2-155[»]
5I9Kelectron microscopy3.50A1-155[»]
5IA9electron microscopy3.50A1-155[»]
ProteinModelPortaliP08011
SMRiP08011
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010579

PTM databases

iPTMnetiP08011
PhosphoSitePlusiP08011

Proteomic databases

PaxDbiP08011
PRIDEiP08011

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010579; ENSRNOP00000010579; ENSRNOG00000007743
GeneIDi171341
KEGGirno:171341
UCSCiRGD:70927 rat

Organism-specific databases

CTDi4257
RGDi70927 Mgst1

Phylogenomic databases

eggNOGiENOG410IXE1 Eukaryota
ENOG4111VJG LUCA
GeneTreeiENSGT00390000011980
HOGENOMiHOG000231759
HOVERGENiHBG052470
InParanoidiP08011
KOiK00799
OMAiFYRMTRK
OrthoDBiEOG091G14I4
PhylomeDBiP08011
TreeFamiTF105327

Enzyme and pathway databases

BRENDAi2.5.1.18 5301
ReactomeiR-RNO-156590 Glutathione conjugation
R-RNO-5423646 Aflatoxin activation and detoxification
R-RNO-6798695 Neutrophil degranulation
SABIO-RKiP08011

Miscellaneous databases

EvolutionaryTraceiP08011
PROiPR:P08011

Gene expression databases

BgeeiENSRNOG00000007743 Expressed in 9 organ(s), highest expression level in liver
ExpressionAtlasiP08011 baseline and differential
GenevisibleiP08011 RN

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
SUPFAMiSSF161084 SSF161084, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMGST1_RAT
AccessioniPrimary (citable) accession number: P08011
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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