UniProtKB - P08004 (CHS1_YEAST)
Protein
Chitin synthase 1
Gene
CHS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer (PubMed:2941152). Required for mitotic division septum formation during adverse conditions (PubMed:2523889).2 Publications
Catalytic activityi
- [(1→4)-N-acetyl-β-D-glucosaminyl](n) + UDP-N-acetyl-α-D-glucosamine = [(1→4)-N-acetyl-β-D-glucosaminyl](n+1) + H+ + UDPEC:2.4.1.16
Activity regulationi
Requires proteolytic activation.
GO - Molecular functioni
- chitin synthase activity Source: SGD
GO - Biological processi
- chitin biosynthetic process Source: InterPro
- fungal-type cell wall organization Source: GO_Central
- septum digestion after cytokinesis Source: SGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell wall biogenesis/degradation |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
TCDBi | 4.D.1.1.17, the putative vectorial glycosyl polymerization (vgp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Chitin synthase 1 (EC:2.4.1.16)Alternative name(s): Chitin-UDP acetyl-glucosaminyl transferase 1 |
Gene namesi | Name:CHS1 Ordered Locus Names:YNL192W ORF Names:N1404 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005136, CHS1 |
VEuPathDBi | FungiDB:YNL192W |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
- cell septum Source: GO_Central
- chitosome Source: SGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 795 – 815 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 833 – 853 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 866 – 886 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 914 – 934 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 942 – 962 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1042 – 1062 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1101 – 1121 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cell lysis during cytokinesis.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3827 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193727 | 1 – 1131 | Chitin synthase 1Add BLAST | 1131 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Modified residuei | 35 | PhosphoserineCombined sources | 1 | |
Modified residuei | 270 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphoserineCombined sources | 1 | |
Modified residuei | 318 | PhosphoserineCombined sources | 1 | |
Modified residuei | 328 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 358 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P08004 |
PaxDbi | P08004 |
PRIDEi | P08004 |
PTM databases
iPTMneti | P08004 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35641, 130 interactors |
DIPi | DIP-4680N |
IntActi | P08004, 8 interactors |
MINTi | P08004 |
STRINGi | 4932.YNL192W |
Chemistry databases
BindingDBi | P08004 |
Miscellaneous databases
RNActi | P08004, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the chitin synthase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota |
HOGENOMi | CLU_004760_3_1_1 |
InParanoidi | P08004 |
OMAi | AKDEING |
Family and domain databases
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR004834, Chitin_synth_fun IPR013616, Chitin_synth_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF01644, Chitin_synth_1, 1 hit PF08407, Chitin_synth_1N, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
P08004-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDQNNRSRN EYHSNRKNEP SYELQNAHSG LFHSSNEELT NRNQRYTNQN
60 70 80 90 100
ASMGSFTPVQ SLQFPEQSQQ TNMLYNGDDG NNNTINDNER DIYGGFVNHH
110 120 130 140 150
RQRPPPATAE YNDVFNTNSQ QLPSEHQYNN VPSYPLPSIN VIQTTPELIH
160 170 180 190 200
NGSQTMATPI ERPFFNENDY YYNNRNSRTS PSIASSSDGY ADQEARPILE
210 220 230 240 250
QPNNNMNSGN IPQYHDQPFG YNNGYHGLQA KDYYDDPEGG YIDQRGDDYQ
260 270 280 290 300
INSYLGRNGE MVDPYDYENS LRHMTPMERR EYLHDDSRPV NDGKEELDSV
310 320 330 340 350
KSGYSHRDLG EYDKDDFSRD DEYDDLNTID KLQFQANGVP ASSSVSSIGS
360 370 380 390 400
KESDIIVSND NLTANRALKR SGTEIRKFKL WNGNFVFDSP ISKTLLDQYA
410 420 430 440 450
TTTENANTLP NEFKFMRYQA VTCEPNQLAE KNFTVRQLKY LTPRETELML
460 470 480 490 500
VVTMYNEDHI LLGRTLKGIM DNVKYMVKKK NSSTWGPDAW KKIVVCIISD
510 520 530 540 550
GRSKINERSL ALLSSLGCYQ DGFAKDEINE KKVAMHVYEH TTMINITNIS
560 570 580 590 600
ESEVSLECNQ GTVPIQLLFC LKEQNQKKIN SHRWAFEGFA ELLRPNIVTL
610 620 630 640 650
LDAGTMPGKD SIYQLWREFR NPNVGGACGE IRTDLGKRFV KLLNPLVASQ
660 670 680 690 700
NFEYKMSNIL DKTTESNFGF ITVLPGAFSA YRFEAVRGQP LQKYFYGEIM
710 720 730 740 750
ENEGFHFFSS NMYLAEDRIL CFEVVTKKNC NWILKYCRSS YASTDVPERV
760 770 780 790 800
PEFILQRRRW LNGSFFASVY SFCHFYRVWS SGHNIGRKLL LTVEFFYLFF
810 820 830 840 850
NTLISWFSLS SFFLVFRILT VSIALAYHSA FNVLSVIFLW LYGICTLSTF
860 870 880 890 900
ILSLGNKPKS TEKFYVLTCV IFAVMMIYMI FCSIFMSVKS FQNILKNDTI
910 920 930 940 950
SFEGLITTEA FRDIVISLGS TYCLYLISSI IYLQPWHMLT SFIQYILLSP
960 970 980 990 1000
SYINVLNIYA FCNVHDLSWG TKGAMANPLG KINTTEDGTF KMEVLVSSSE
1010 1020 1030 1040 1050
IQANYDKYLK VLNDFDPKSE SRPTEPSYDE KKTGYYANVR SLVIIFWVIT
1060 1070 1080 1090 1100
NFIIVAVVLE TGGIADYIAM KSISTDDTLE TAKKAEIPLM TSKASIYFNV
1110 1120 1130
ILWLVALSAL IRFIGCSIYM IVRFFKKVTF R
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 815 | V → F in AAA34491 (PubMed:2941152).Curated | 1 | |
Sequence conflicti | 815 | V → F in CAA96086 (PubMed:9169873).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14045 Genomic DNA Translation: AAA34491.1 Z71468 Genomic DNA Translation: CAA96086.1 BK006947 Genomic DNA Translation: DAA10361.2 |
PIRi | A23944 |
RefSeqi | NP_014207.2, NM_001183030.2 |
Genome annotation databases
EnsemblFungii | YNL192W_mRNA; YNL192W; YNL192W |
GeneIDi | 855529 |
KEGGi | sce:YNL192W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14045 Genomic DNA Translation: AAA34491.1 Z71468 Genomic DNA Translation: CAA96086.1 BK006947 Genomic DNA Translation: DAA10361.2 |
PIRi | A23944 |
RefSeqi | NP_014207.2, NM_001183030.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35641, 130 interactors |
DIPi | DIP-4680N |
IntActi | P08004, 8 interactors |
MINTi | P08004 |
STRINGi | 4932.YNL192W |
Chemistry databases
BindingDBi | P08004 |
ChEMBLi | CHEMBL3827 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
TCDBi | 4.D.1.1.17, the putative vectorial glycosyl polymerization (vgp) family |
PTM databases
iPTMneti | P08004 |
Proteomic databases
MaxQBi | P08004 |
PaxDbi | P08004 |
PRIDEi | P08004 |
Genome annotation databases
EnsemblFungii | YNL192W_mRNA; YNL192W; YNL192W |
GeneIDi | 855529 |
KEGGi | sce:YNL192W |
Organism-specific databases
SGDi | S000005136, CHS1 |
VEuPathDBi | FungiDB:YNL192W |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota |
HOGENOMi | CLU_004760_3_1_1 |
InParanoidi | P08004 |
OMAi | AKDEING |
Miscellaneous databases
PROi | PR:P08004 |
RNActi | P08004, protein |
Family and domain databases
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR004834, Chitin_synth_fun IPR013616, Chitin_synth_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF01644, Chitin_synth_1, 1 hit PF08407, Chitin_synth_1N, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS1_YEAST | |
Accessioni | P08004Primary (citable) accession number: P08004 Secondary accession number(s): D6W0Z5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | September 21, 2011 | |
Last modified: | February 10, 2021 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - SIMILARITY comments
Index of protein domains and families