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Protein

Protein disulfide-isomerase A4

Gene

Pdia4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A4 (EC:5.3.4.1)
Alternative name(s):
Endoplasmic reticulum resident protein 72
Short name:
ER protein 72
Short name:
ERp-72
Short name:
ERp72
Gene namesi
Name:Pdia4
Synonyms:Cai, Erp72
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104864 Pdia4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003423021 – 638Protein disulfide-isomerase A4Add BLAST618

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi84 ↔ 87Redox-activePROSITE-ProRule annotation
Disulfide bondi199 ↔ 202Redox-activePROSITE-ProRule annotation
Modified residuei359N6-acetyllysineBy similarity1
Disulfide bondi548 ↔ 551Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

EPDiP08003
MaxQBiP08003
PaxDbiP08003
PeptideAtlasiP08003
PRIDEiP08003

PTM databases

iPTMnetiP08003
PhosphoSitePlusiP08003
SwissPalmiP08003

Expressioni

Gene expression databases

CleanExiMM_PDIA4

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Protein-protein interaction databases

BioGridi198446, 1 interactor
CORUMiP08003
IntActiP08003, 5 interactors
MINTiP08003
STRINGi10090.ENSMUSP00000076521

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP08003
SMRiP08003
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08003

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 162Thioredoxin 1PROSITE-ProRule annotationAdd BLAST142
Domaini162 – 294Thioredoxin 2PROSITE-ProRule annotationAdd BLAST133
Domaini498 – 629Thioredoxin 3PROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi635 – 638Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 50Asp/Glu-rich (acidic)Add BLAST12

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP08003
KOiK09582
PhylomeDBiP08003
TreeFamiTF106382

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR017068 Protein_diS-isomerase_A4
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 3 hits
PIRSFiPIRSF036862 Disulphide_isom_A4, 1 hit
SUPFAMiSSF52833 SSF52833, 5 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 3 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 3 hits
PS51352 THIOREDOXIN_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P08003-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLRKAWLLV LLLALTQLLA AASAGDAHED TSDTENATEE EEEEDDDDLE
60 70 80 90 100
VKEENGVWVL NDGNFDNFVA DKDTVLLEFY APWCGHCKQF APEYEKIAST
110 120 130 140 150
LKDNDPPIAV AKIDATSASM LASKFDVSGY PTIKILKKGQ AVDYDGSRTQ
160 170 180 190 200
EEIVAKVREV SQPDWTPPPE VTLSLTKDNF DDVVNNADII LVEFYAPWCG
210 220 230 240 250
HCKKLAPEYE KAAKELSKRS PPIPLAKVDA TEQTDLAKRF DVSGYPTLKI
260 270 280 290 300
FRKGRPFDYN GPREKYGIVD YMIEQSGPPS KEILTLKQVQ EFLKDGDDVV
310 320 330 340 350
IIGLFQGDGD PAYLQYQDAA NNLREDYKFH HTFSPEIAKF LKVSLGKLVL
360 370 380 390 400
THPEKFQSKY EPRFHVMDVQ GSTEASAIKD YVVKHALPLV GHRKTSNDAK
410 420 430 440 450
RYSKRPLVVV YYSVDFSFDY RAATQFWRNK VLEVAKDFPE YTFAIADEED
460 470 480 490 500
YATEVKDLGL SESGEDVNAA ILDESGKKFA MEPEEFDSDT LREFVTAFKK
510 520 530 540 550
GKLKPVIKSQ PVPKNNKGPV KVVVGKTFDA IVMDPKKDVL IEFYAPWCGH
560 570 580 590 600
CKQLEPIYTS LGKKYKGQKD LVIAKMDATA NDITNDQYKV EGFPTIYFAP
610 620 630
SGDKKNPIKF EGGNRDLEHL SKFIDEHATK RSRTKEEL
Length:638
Mass (Da):71,982
Last modified:November 30, 2010 - v3
Checksum:i2DDE2CE80BD77F6E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0Z1A0A0R4J0Z1_MOUSE
Protein disulfide-isomerase A4
Pdia4
641Annotation score:

Sequence cautioni

The sequence CAA68777 differs from that shown. Reason: Frameshift at position 544.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28H → Q in AAA39907 (PubMed:2295602).Curated1
Sequence conflicti28H → Q in BAE27045 (PubMed:16141072).Curated1
Sequence conflicti28H → Q in AAI41079 (PubMed:15489334).Curated1
Sequence conflicti39E → EEEE in BAC25863 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE41134 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE36446 (PubMed:16141072).Curated1
Sequence conflicti39E → EEEE in BAE29664 (PubMed:16141072).Curated1
Sequence conflicti468N → S in BAC25863 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05186 mRNA Translation: AAA39907.1
AK028292 mRNA Translation: BAC25863.1
AK146288 mRNA Translation: BAE27045.1
AK150566 mRNA Translation: BAE29664.1
AK161534 mRNA Translation: BAE36446.1
AK169387 mRNA Translation: BAE41134.1
BC066857 mRNA Translation: AAH66857.1
BC141078 mRNA Translation: AAI41079.1
Y00884 mRNA Translation: CAA68777.1 Frameshift.
PIRiB34930 ISMSER
S06318
RefSeqiNP_033917.2, NM_009787.2
UniGeneiMm.2442

Genome annotation databases

GeneIDi12304
KEGGimmu:12304
UCSCiuc009btf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05186 mRNA Translation: AAA39907.1
AK028292 mRNA Translation: BAC25863.1
AK146288 mRNA Translation: BAE27045.1
AK150566 mRNA Translation: BAE29664.1
AK161534 mRNA Translation: BAE36446.1
AK169387 mRNA Translation: BAE41134.1
BC066857 mRNA Translation: AAH66857.1
BC141078 mRNA Translation: AAI41079.1
Y00884 mRNA Translation: CAA68777.1 Frameshift.
PIRiB34930 ISMSER
S06318
RefSeqiNP_033917.2, NM_009787.2
UniGeneiMm.2442

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DJ1NMR-A46-172[»]
2DJ2NMR-A171-277[»]
2DJ3NMR-A519-638[»]
ProteinModelPortaliP08003
SMRiP08003
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198446, 1 interactor
CORUMiP08003
IntActiP08003, 5 interactors
MINTiP08003
STRINGi10090.ENSMUSP00000076521

PTM databases

iPTMnetiP08003
PhosphoSitePlusiP08003
SwissPalmiP08003

Proteomic databases

EPDiP08003
MaxQBiP08003
PaxDbiP08003
PeptideAtlasiP08003
PRIDEiP08003

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12304
KEGGimmu:12304
UCSCiuc009btf.1 mouse

Organism-specific databases

CTDi9601
MGIiMGI:104864 Pdia4

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP08003
KOiK09582
PhylomeDBiP08003
TreeFamiTF106382

Miscellaneous databases

ChiTaRSiPdia4 mouse
EvolutionaryTraceiP08003
PROiPR:P08003
SOURCEiSearch...

Gene expression databases

CleanExiMM_PDIA4

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR017068 Protein_diS-isomerase_A4
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 3 hits
PIRSFiPIRSF036862 Disulphide_isom_A4, 1 hit
SUPFAMiSSF52833 SSF52833, 5 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 3 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 3 hits
PS51352 THIOREDOXIN_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA4_MOUSE
AccessioniPrimary (citable) accession number: P08003
Secondary accession number(s): P15841
, Q3TT79, Q3UJW0, Q6NXW4, Q8BMT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 30, 2010
Last modified: June 20, 2018
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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