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UniProtKB - P07984 (GUNA_CELFI)
Protein
Endoglucanase A
Gene
cenA
Organism
Cellulomonas fimi
Status
Functioni
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 247 | PROSITE-ProRule annotation | 1 | |
Active sitei | 283 | Proton donorPROSITE-ProRule annotation | 1 | |
Active sitei | 423 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
- polysaccharide binding Source: InterPro
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Enzyme and pathway databases
SABIO-RKi | P07984 |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 GH6, Glycoside Hydrolase Family 6 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase A (EC:3.2.1.4)Alternative name(s): Cellulase A Endo-1,4-beta-glucanase A |
Gene namesi | Name:cenA |
Organismi | Cellulomonas fimi |
Taxonomic identifieri | 1708 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Micrococcales › Cellulomonadaceae › Cellulomonas |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Tat-type signalPROSITE-ProRule annotationAdd BLAST | 31 | |
ChainiPRO_0000007903 | 32 – 449 | Endoglucanase AAdd BLAST | 418 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 35 ↔ 134 | 1 Publication | ||
Disulfide bondi | 248 ↔ 291 | 1 Publication | ||
Disulfide bondi | 390 ↔ 426 | 1 Publication |
Post-translational modificationi
The linker region (also termed 'hinge') may be a potential site for proteolysis.
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | P07984 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 137 | CBM2PROSITE-ProRule annotationAdd BLAST | 106 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 127 – 170 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 139 – 168 | Linker ('hinge') (Pro-Thr box)Add BLAST | 30 | |
Regioni | 438 – 449 | CatalyticAdd BLAST | 12 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 127 – 141 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 142 – 167 | Pro residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 6 (cellulase B) family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 2.60.40.290, 1 hit 3.20.20.40, 1 hit |
InterProi | View protein in InterPro IPR016288, Beta_cellobiohydrolase IPR036434, Beta_cellobiohydrolase_sf IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001524, Glyco_hydro_6_CS IPR006311, TAT_signal |
PANTHERi | PTHR34876, PTHR34876, 1 hit |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF01341, Glyco_hydro_6, 1 hit |
PIRSFi | PIRSF001100, Beta_cellobiohydrolase, 1 hit |
PRINTSi | PR00733, GLHYDRLASE6 |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit |
SUPFAMi | SSF49384, SSF49384, 1 hit SSF51989, SSF51989, 1 hit |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS00655, GLYCOSYL_HYDROL_F6_1, 1 hit PS00656, GLYCOSYL_HYDROL_F6_2, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07984-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTRRTAAAL LAAAAVAVGG LTALTTTAAQ AAPGCRVDYA VTNQWPGGFG
60 70 80 90 100
ANVTITNLGD PVSSWKLDWT YTAGQRIQQL WNGTASTNGG QVSVTSLPWN
110 120 130 140 150
GSIPTGGTAS FGFNGSWAGS NPTPASFSLN GTTCTGTVPT TSPTPTPTPT
160 170 180 190 200
TPTPTPTPTP TPTPTVTPQP TSGFYVDPTT QGYRAWQAAS GTDKALLEKI
210 220 230 240 250
ALTPQAYWVG NWADASHAQA EVADYTGRAV AAGKTPMLVV YAIPGRDCGS
260 270 280 290 300
HSGGGVSESE YARWVDTVAQ GIKGNPIVIL EPDALAQLGD CSGQGDRVGF
310 320 330 340 350
LKYAAKSLTL KGARVYIDAG HAKWLSVDTP VNRLNQVGFE YAVGFALNTS
360 370 380 390 400
NYQTTADSKA YGQQISQRLG GKKFVIDTSR NGNGSNGEWC NPRGRALGER
410 420 430 440
PVAVNDGSGL DALLWVKLPG ESDGACNGGP AAGQWWQEIA LEMARNARW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15823 Genomic DNA Translation: AAA23084.1 |
PIRi | A24993 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15823 Genomic DNA Translation: AAA23084.1 |
PIRi | A24993 |
3D structure databases
AlphaFoldDBi | P07984 |
SMRi | P07984 |
ModBasei | Search... |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 GH6, Glycoside Hydrolase Family 6 |
Proteomic databases
PRIDEi | P07984 |
Enzyme and pathway databases
SABIO-RKi | P07984 |
Family and domain databases
Gene3Di | 2.60.40.290, 1 hit 3.20.20.40, 1 hit |
InterProi | View protein in InterPro IPR016288, Beta_cellobiohydrolase IPR036434, Beta_cellobiohydrolase_sf IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001524, Glyco_hydro_6_CS IPR006311, TAT_signal |
PANTHERi | PTHR34876, PTHR34876, 1 hit |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF01341, Glyco_hydro_6, 1 hit |
PIRSFi | PIRSF001100, Beta_cellobiohydrolase, 1 hit |
PRINTSi | PR00733, GLHYDRLASE6 |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit |
SUPFAMi | SSF49384, SSF49384, 1 hit SSF51989, SSF51989, 1 hit |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS00655, GLYCOSYL_HYDROL_F6_1, 1 hit PS00656, GLYCOSYL_HYDROL_F6_2, 1 hit PS51318, TAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNA_CELFI | |
Accessioni | P07984Primary (citable) accession number: P07984 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | May 25, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families