UniProtKB - P07943 (ALDR_RAT)
Protein
Aldo-keto reductase family 1 member B1
Gene
Akr1b1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).By similarity
Catalytic activityi
- EC:1.1.1.21By similarity
- EC:1.1.1.300By similarity
- EC:1.1.1.372By similarity
- EC:1.1.1.372By similarity
- EC:1.1.1.54By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | Proton donorBy similarity | 1 | |
Sitei | 78 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 111 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 19 | NADPSequence analysis | 10 | |
Nucleotide bindingi | 211 – 273 | NADPBy similarityAdd BLAST | 63 |
GO - Molecular functioni
- alditol:NADP+ 1-oxidoreductase activity Source: RGD
- allyl-alcohol dehydrogenase activity Source: UniProtKB-EC
- D-threo-aldose 1-dehydrogenase activity Source: InterPro
- glyceraldehyde oxidoreductase activity Source: RGD
- glycerol dehydrogenase [NADP+] activity Source: RHEA
- NADP-retinol dehydrogenase activity Source: UniProtKB-EC
- prostaglandin H2 endoperoxidase reductase activity Source: RHEA
- retinal dehydrogenase activity Source: UniProtKB
GO - Biological processi
- cellular hyperosmotic salinity response Source: RGD
- cellular response to methylglyoxal Source: RGD
- cellular response to peptide Source: RGD
- daunorubicin metabolic process Source: RGD
- doxorubicin metabolic process Source: RGD
- epithelial cell maturation Source: RGD
- inner medullary collecting duct development Source: RGD
- maternal process involved in female pregnancy Source: RGD
- metanephric collecting duct development Source: RGD
- monosaccharide metabolic process Source: RGD
- naphthalene metabolic process Source: RGD
- negative regulation of apoptotic process Source: RGD
- norepinephrine metabolic process Source: RGD
- positive regulation of receptor signaling pathway via JAK-STAT Source: RGD
- positive regulation of smooth muscle cell proliferation Source: RGD
- regulation of urine volume Source: RGD
- renal water homeostasis Source: RGD
- response to organic substance Source: RGD
- response to thyroid hormone Source: RGD
- response to water deprivation Source: RGD
- retinoid metabolic process Source: UniProtKB
- sorbitol biosynthetic process Source: RGD
- stress-activated protein kinase signaling cascade Source: RGD
- tissue homeostasis Source: RGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.21, 5301 |
Reactomei | R-RNO-196108, Pregnenolone biosynthesis R-RNO-5652227, Fructose biosynthesis |
SABIO-RKi | P07943 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Akr1b1 Synonyms:Akr1b4, Aldr1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2092, Akr1b1 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: RGD
Extracellular region or secreted
- extracellular space Source: RGD
Lysosome
- mast cell granule Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
Other locations
- paranodal junction Source: RGD
- perinuclear region of cytoplasm Source: RGD
- plasma membrane bounded cell projection cytoplasm Source: RGD
- Schmidt-Lanterman incisure Source: RGD
- Schwann cell microvillus Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000124627 | 2 – 316 | Aldo-keto reductase family 1 member B1Add BLAST | 315 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 3 | PhosphoserineCombined sources | 1 | |
Modified residuei | 95 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 222 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 263 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P07943 |
PaxDbi | P07943 |
PRIDEi | P07943 |
PTM databases
iPTMneti | P07943 |
PhosphoSitePlusi | P07943 |
SwissPalmi | P07943 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000009513, Expressed in female gonad and 20 other tissues |
Genevisiblei | P07943, RN |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 246382, 2 interactors |
STRINGi | 10116.ENSRNOP00000012879 |
Chemistry databases
BindingDBi | P07943 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153272 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | P07943 |
OMAi | VWNHLHE |
OrthoDBi | 1016440at2759 |
PhylomeDBi | P07943 |
TreeFami | TF106492 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P07943-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASHLELNNG TKMPTLGLGT WKSPPGQVTE AVKVAIDMGY RHIDCAQVYQ
60 70 80 90 100
NEKEVGVALQ EKLKEQVVKR QDLFIVSKLW CTFHDQSMVK GACQKTLSDL
110 120 130 140 150
QLDYLDLYLI HWPTGFKPGP DYFPLDASGN VIPSDTDFVD TWTAMEQLVD
160 170 180 190 200
EGLVKAIGVS NFNPLQIERI LNKPGLKYKP AVNQIECHPY LTQEKLIEYC
210 220 230 240 250
HCKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKEIAAK YNKTTAQVLI
260 270 280 290 300
RFPIQRNLVV IPKSVTPARI AENFKVFDFE LSNEDMATLL SYNRNWRVCA
310
LMSCAKHKDY PFHAEV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05884 mRNA Translation: CAA29308.1 M60322 Genomic DNA Translation: AAA40721.1 BC062034 mRNA Translation: AAH62034.1 |
PIRi | A60603 |
RefSeqi | NP_036630.1, NM_012498.1 |
Genome annotation databases
Ensembli | ENSRNOT00000012879; ENSRNOP00000012879; ENSRNOG00000009513 |
GeneIDi | 24192 |
KEGGi | rno:24192 |
UCSCi | RGD:2092, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05884 mRNA Translation: CAA29308.1 M60322 Genomic DNA Translation: AAA40721.1 BC062034 mRNA Translation: AAH62034.1 |
PIRi | A60603 |
RefSeqi | NP_036630.1, NM_012498.1 |
3D structure databases
SMRi | P07943 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246382, 2 interactors |
STRINGi | 10116.ENSRNOP00000012879 |
Chemistry databases
BindingDBi | P07943 |
ChEMBLi | CHEMBL2622 |
DrugCentrali | P07943 |
PTM databases
iPTMneti | P07943 |
PhosphoSitePlusi | P07943 |
SwissPalmi | P07943 |
Proteomic databases
jPOSTi | P07943 |
PaxDbi | P07943 |
PRIDEi | P07943 |
Genome annotation databases
Ensembli | ENSRNOT00000012879; ENSRNOP00000012879; ENSRNOG00000009513 |
GeneIDi | 24192 |
KEGGi | rno:24192 |
UCSCi | RGD:2092, rat |
Organism-specific databases
CTDi | 231 |
RGDi | 2092, Akr1b1 |
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153272 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | P07943 |
OMAi | VWNHLHE |
OrthoDBi | 1016440at2759 |
PhylomeDBi | P07943 |
TreeFami | TF106492 |
Enzyme and pathway databases
BRENDAi | 1.1.1.21, 5301 |
Reactomei | R-RNO-196108, Pregnenolone biosynthesis R-RNO-5652227, Fructose biosynthesis |
SABIO-RKi | P07943 |
Miscellaneous databases
PROi | PR:P07943 |
Gene expression databases
Bgeei | ENSRNOG00000009513, Expressed in female gonad and 20 other tissues |
Genevisiblei | P07943, RN |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALDR_RAT | |
Accessioni | P07943Primary (citable) accession number: P07943 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families