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Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).By similarity

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
ATP + [tau protein] = ADP + [tau protein] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei150Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19 3474
ReactomeiR-MMU-70221 Glycogen breakdown (glycogenolysis)

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Gene namesi
Name:Phkg1
Synonyms:Phkg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97579 Phkg1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865091 – 388Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformAdd BLAST388

Proteomic databases

MaxQBiP07934
PaxDbiP07934
PeptideAtlasiP07934
PRIDEiP07934

PTM databases

PhosphoSitePlusiP07934

Expressioni

Gene expression databases

BgeeiENSMUSG00000025537 Expressed in 122 organ(s), highest expression level in triceps surae
CleanExiMM_PHKG1
ExpressionAtlasiP07934 baseline and differential
GenevisibleiP07934 MM

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026617

Structurei

3D structure databases

ProteinModelPortaliP07934
SMRiP07934
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni303 – 327Calmodulin-binding (domain-N)Add BLAST25
Regioni343 – 367Calmodulin-binding (domain-C)Add BLAST25

Domaini

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0599 Eukaryota
ENOG410Y7VX LUCA
GeneTreeiENSGT00840000129744
HOGENOMiHOG000233016
HOVERGENiHBG106193
InParanoidiP07934
KOiK00871
OMAiQGFYENY
OrthoDBiEOG091G07XZ
PhylomeDBiP07934
TreeFamiTF320349

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR002291 Phosph_kin_gamma
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01049 PHOSPHBKNASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

P07934-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRDDALPDS HSAQTFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI
60 70 80 90 100
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF
110 120 130 140 150
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVICT LHKLNIVHRD
160 170 180 190 200
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM
210 220 230 240 250
DDGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG
260 270 280 290 300
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS
310 320 330 340 350
PRGKFKVICL TVLASVKIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY
360 370 380
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF
Length:388
Mass (Da):44,960
Last modified:January 23, 2007 - v3
Checksum:i96E1256891E43718
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z1B5D3Z1B5_MOUSE
Phosphorylase b kinase gamma cataly...
Phkg1
95Annotation score:
D3Z472D3Z472_MOUSE
Phosphorylase b kinase gamma cataly...
Phkg1
143Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313L → V in AAA39926 (PubMed:3472241).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08059, L08057, L08058 Genomic DNA Translation: AAB59721.1
M16216 mRNA Translation: AAA39926.1
J03293 mRNA Translation: AAA39925.1
S60494 Genomic DNA No translation available.
S60492 Genomic DNA No translation available.
S60493 Genomic DNA No translation available.
BC055102 mRNA Translation: AAH55102.1
CCDSiCCDS19701.1
PIRiA29872
RefSeqiNP_035209.1, NM_011079.3
UniGeneiMm.3159

Genome annotation databases

EnsembliENSMUST00000026617; ENSMUSP00000026617; ENSMUSG00000025537
GeneIDi18682
KEGGimmu:18682
UCSCiuc008ztm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPHKG1_MOUSE
AccessioniPrimary (citable) accession number: P07934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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