UniProtKB - P07913 (TDH_ECOLI)
Protein
L-threonine 3-dehydrogenase
Gene
tdh
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate. To a lesser extent, also catalyzes the oxidation of D-allo-threonine and L-threonine amide, but not that of D-threonine and L-allothreonine. Cannot utilize NADP+ instead of NAD+.1 Publication
Catalytic activityi
- EC:1.1.1.103UniRule annotation1 Publication
Cofactori
Zn2+UniRule annotation1 Publication1 Publication, Co2+1 Publication, Cd2+1 PublicationNote: Binds 2 Zn2+ ions per subunit. Co2+ and Cd2+ can exchange for Zn2+ (PubMed:2007567). Probably contains one structural ion and one catalytic ion that seems to be less tightly bound at the site (PubMed:9784233).UniRule annotation1 Publication1 Publication
Activity regulationi
Is totally inhibited by EDTA in vitro.1 Publication
Kineticsi
- KM=1.43 mM for L-threonine (at 37 degrees Celsius and pH 8.4)1 Publication
- KM=0.19 mM for NAD+ (at 37 degrees Celsius and pH 8.4)1 Publication
- Vmax=57 µmol/min/mg enzyme for the NAD+ oxidation of L-threonine1 Publication
pH dependencei
Optimum pH is 10.3. At pH 9.0 and 11.0, the activity is 35% and only 6%, respectively, of the optimal level.1 Publication
: L-threonine degradation via oxydo-reductase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycine from L-threonine.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- L-threonine 3-dehydrogenase (tdh), L-threonine 3-dehydrogenase (tdh)
- 2-amino-3-ketobutyrate coenzyme A ligase (kbl), 2-amino-3-ketobutyrate coenzyme A ligase (kbl)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 38 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Active sitei | 40 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 43 | Charge relay systemUniRule annotation | 1 | |
Metal bindingi | 63 | Zinc 1; via tele nitrogen; catalyticUniRule annotation | 1 | |
Metal bindingi | 64 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 93 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 96 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 99 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 107 | Zinc 2UniRule annotation | 1 | |
Sitei | 148 | Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atomUniRule annotation | 1 | |
Binding sitei | 175 | NAD; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 195 | NADUniRule annotation | 1 | |
Binding sitei | 200 | NADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 262 – 264 | NADUniRule annotation | 3 | |
Nucleotide bindingi | 286 – 287 | NADUniRule annotation | 2 |
GO - Molecular functioni
- cadmium ion binding Source: EcoliWiki
- ferrous iron binding Source: EcoCyc
- L-threonine 3-dehydrogenase activity Source: EcoCyc
- manganese ion binding Source: EcoliWiki
- oxidoreductase activity Source: EcoliWiki
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- L-serine biosynthetic process Source: EcoliWiki
- L-threonine catabolic process to glycine Source: UniProtKB-UniPathway
- threonine catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Cobalt, Metal-binding, NAD, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:THREODEHYD-MONOMER MetaCyc:THREODEHYD-MONOMER |
SABIO-RKi | P07913 |
UniPathwayi | UPA00046;UER00505 |
Names & Taxonomyi
Protein namesi | Recommended name: L-threonine 3-dehydrogenaseUniRule annotation (EC:1.1.1.103UniRule annotation1 Publication)Short name: TDH1 PublicationUniRule annotation Alternative name(s): L-threonine dehydrogenase2 Publications |
Gene namesi | Name:tdh1 Publication Ordered Locus Names:b3616, JW3591 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 38 | C → D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+). 1 Publication | 1 | |
Mutagenesisi | 38 | C → S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+). 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160837 | 1 – 341 | L-threonine 3-dehydrogenaseAdd BLAST | 341 |
Proteomic databases
jPOSTi | P07913 |
PaxDbi | P07913 |
PRIDEi | P07913 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263307, 26 interactors 852446, 1 interactor |
DIPi | DIP-6855N |
IntActi | P07913, 6 interactors |
STRINGi | 511145.b3616 |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1063, Bacteria |
HOGENOMi | CLU_026673_11_0_6 |
InParanoidi | P07913 |
PhylomeDBi | P07913 |
Family and domain databases
HAMAPi | MF_00627, Thr_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR004627, L-Threonine_3-DHase IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00692, tdh, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
P07913-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKALSKLKAE EGIWMTDVPV PELGHNDLLI KIRKTAICGT DVHIYNWDEW
60 70 80 90 100
SQKTIPVPMV VGHEYVGEVV GIGQEVKGFK IGDRVSGEGH ITCGHCRNCR
110 120 130 140 150
GGRTHLCRNT IGVGVNRPGC FAEYLVIPAF NAFKIPDNIS DDLAAIFDPF
160 170 180 190 200
GNAVHTALSF DLVGEDVLVS GAGPIGIMAA AVAKHVGARN VVITDVNEYR
210 220 230 240 250
LELARKMGIT RAVNVAKENL NDVMAELGMT EGFDVGLEMS GAPPAFRTML
260 270 280 290 300
DTMNHGGRIA MLGIPPSDMS IDWTKVIFKG LFIKGIYGRE MFETWYKMAA
310 320 330 340
LIQSGLDLSP IITHRFSIDD FQKGFDAMRS GQSGKVILSW D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06690 Genomic DNA Translation: CAA29884.1 U00039 Genomic DNA Translation: AAB18593.1 U00096 Genomic DNA Translation: AAC76640.1 AP009048 Genomic DNA Translation: BAE77676.1 |
PIRi | A33276, DEECTH |
RefSeqi | NP_418073.1, NC_000913.3 WP_000646007.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76640; AAC76640; b3616 BAE77676; BAE77676; BAE77676 |
GeneIDi | 948139 |
KEGGi | ecj:JW3591 eco:b3616 |
PATRICi | fig|1411691.4.peg.3090 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06690 Genomic DNA Translation: CAA29884.1 U00039 Genomic DNA Translation: AAB18593.1 U00096 Genomic DNA Translation: AAC76640.1 AP009048 Genomic DNA Translation: BAE77676.1 |
PIRi | A33276, DEECTH |
RefSeqi | NP_418073.1, NC_000913.3 WP_000646007.1, NZ_LN832404.1 |
3D structure databases
SMRi | P07913 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263307, 26 interactors 852446, 1 interactor |
DIPi | DIP-6855N |
IntActi | P07913, 6 interactors |
STRINGi | 511145.b3616 |
Proteomic databases
jPOSTi | P07913 |
PaxDbi | P07913 |
PRIDEi | P07913 |
Genome annotation databases
EnsemblBacteriai | AAC76640; AAC76640; b3616 BAE77676; BAE77676; BAE77676 |
GeneIDi | 948139 |
KEGGi | ecj:JW3591 eco:b3616 |
PATRICi | fig|1411691.4.peg.3090 |
Organism-specific databases
EchoBASEi | EB0986 |
Phylogenomic databases
eggNOGi | COG1063, Bacteria |
HOGENOMi | CLU_026673_11_0_6 |
InParanoidi | P07913 |
PhylomeDBi | P07913 |
Enzyme and pathway databases
UniPathwayi | UPA00046;UER00505 |
BioCyci | EcoCyc:THREODEHYD-MONOMER MetaCyc:THREODEHYD-MONOMER |
SABIO-RKi | P07913 |
Miscellaneous databases
PROi | PR:P07913 |
Family and domain databases
HAMAPi | MF_00627, Thr_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR004627, L-Threonine_3-DHase IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00692, tdh, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDH_ECOLI | |
Accessioni | P07913Primary (citable) accession number: P07913 Secondary accession number(s): Q2M7T0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families