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Protein

Heterogeneous nuclear ribonucleoproteins C1/C2

Gene

HNRNPC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).5 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • mRNA 3'-UTR binding Source: UniProtKB
  • N6-methyladenosine-containing RNA binding Source: UniProtKB
  • nucleosomal DNA binding Source: UniProtKB
  • poly(U) RNA binding Source: UniProtKB
  • RNA binding Source: BHF-UCL
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: UniProtKB
  • telomerase RNA binding Source: BHF-UCL

GO - Biological processi

  • 3'-UTR-mediated mRNA stabilization Source: UniProtKB
  • ATP-dependent chromatin remodeling Source: UniProtKB
  • mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of telomere maintenance via telomerase Source: BHF-UCL
  • osteoblast differentiation Source: UniProtKB
  • RNA metabolic process Source: Reactome
  • RNA splicing Source: ProtInc

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
SIGNORiP07910

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoproteins C1/C2
Short name:
hnRNP C1/C2
Gene namesi
Name:HNRNPC
Synonyms:HNRPC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000092199.17
HGNCiHGNC:5035 HNRNPC
MIMi164020 gene
neXtProtiNX_P07910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197K → R: No effect on sumoylation. 1 Publication1
Mutagenesisi250K → R: Loss of sumoylation. 1 Publication1

Organism-specific databases

DisGeNETi3183
OpenTargetsiENSG00000092199
PharmGKBiPA162391217

Chemistry databases

ChEMBLiCHEMBL2216742

Polymorphism and mutation databases

BioMutaiHNRNPC
DMDMi108935845

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000818442 – 306Heterogeneous nuclear ribonucleoproteins C1/C2Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki8Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki94Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei109PhosphothreonineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei115PhosphoserineCombined sources1
Modified residuei121PhosphoserineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei166PhosphoserineCombined sources1
Modified residuei176N6-acetyllysine; alternateBy similarity1
Cross-linki176Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki223Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki229Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki232Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki232Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei233PhosphoserineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei239PhosphoserineCombined sources1
Modified residuei241PhosphoserineCombined sources1
Cross-linki243Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki250Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki250Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei253PhosphoserineCombined sources1 Publication1
Modified residuei260PhosphoserineCombined sources1 Publication1
Modified residuei299PhosphoserineCombined sources1 Publication1
Modified residuei306PhosphoserineCombined sources1
Isoform C1 (identifier: P07910-2)
Modified residuei107PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Isoform 4 (identifier: P07910-4)
Modified residuei107PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.1 Publication
Sumoylated. Sumoylation reduces affinity for mRNA.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP07910
MaxQBiP07910
PaxDbiP07910
PeptideAtlasiP07910
PRIDEiP07910
ProteomicsDBi52034
52035 [P07910-2]
52036 [P07910-3]
52037 [P07910-4]
TopDownProteomicsiP07910-1 [P07910-1]
P07910-2 [P07910-2]

2D gel databases

SWISS-2DPAGEiP07910

PTM databases

iPTMnetiP07910
PhosphoSitePlusiP07910
SwissPalmiP07910

Expressioni

Gene expression databases

BgeeiENSG00000092199
ExpressionAtlasiP07910 baseline and differential
GenevisibleiP07910 HS

Organism-specific databases

HPAiCAB005223
CAB075755
CAB075756
HPA051075

Interactioni

Subunit structurei

Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with DHX9; this interaction is direct, enhanced probably by their concomitant binding to RNA and mediates the attachment to actin filaments (PubMed:11687588).6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi109424, 192 interactors
CORUMiP07910
DIPiDIP-29854N
IntActiP07910, 136 interactors
MINTiP07910
STRINGi9606.ENSP00000319690

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Beta strandi18 – 23Combined sources6
Turni25 – 27Combined sources3
Helixi30 – 35Combined sources6
Turni38 – 40Combined sources3
Beta strandi43 – 49Combined sources7
Beta strandi51 – 59Combined sources9
Helixi60 – 68Combined sources9
Turni69 – 72Combined sources4
Beta strandi81 – 84Combined sources4
Beta strandi94 – 96Combined sources3
Helixi194 – 216Combined sources23

3D structure databases

ProteinModelPortaliP07910
SMRiP07910
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07910

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 87RRMPROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili190 – 238Sequence analysisAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi155 – 161Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 303Asp/Glu-rich (acidic)Add BLAST123

Sequence similaritiesi

Belongs to the RRM HNRPC family. RALY subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00390000006718
HOVERGENiHBG002302
InParanoidiP07910
KOiK12884
OMAiHRSKTSK
OrthoDBiEOG091G0O1Q
PhylomeDBiP07910
TreeFamiTF330974

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR017347 hnRNP_C
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PIRSFiPIRSF037992 hnRNP-C_Raly, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C2 (identifier: P07910-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK
60 70 80 90 100
GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS
110 120 130 140 150
AAEMYGSVTE HPSPSPLLSS SFDLDYDFQR DYYDRMYSYP ARVPPPPPIA
160 170 180 190 200
RAVVPSKRQR VSGNTSRRGK SGFNSKSGQR GSSKSGKLKG DDLQAIKKEL
210 220 230 240 250
TQIKQKVDSL LENLEKIEKE QSKQAVEMKN DKSEEEQSSS SVKKDETNVK
260 270 280 290 300
MESEGGADDS AEEGDLLDDD DNEDRGDDQL ELIKDDEKEA EEGEDDRDSA

NGEDDS
Length:306
Mass (Da):33,670
Last modified:June 13, 2006 - v4
Checksum:i17BBC78690C69C5C
GO
Isoform C1 (identifier: P07910-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-120: Missing.

Show »
Length:293
Mass (Da):32,338
Checksum:iB90DDFD06A77FEE8
GO
Isoform 3 (identifier: P07910-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-119: KYGKIVGCSV...EHPSPSPLLS → N

Show »
Length:226
Mass (Da):25,230
Checksum:iBE6EC8C7A9FD7114
GO
Isoform 4 (identifier: P07910-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-120: Missing.
     153-195: Missing.

Show »
Length:250
Mass (Da):27,822
Checksum:i543388C3EC431836
GO

Sequence cautioni

The sequence BAD92764 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110E → G in AAH08423 (PubMed:15489334).Curated1
Sequence conflicti217I → M (PubMed:2557628).Curated1
Sequence conflicti217I → M (PubMed:3110598).Curated1
Sequence conflicti224Q → R in AAH08364 (PubMed:15489334).Curated1
Sequence conflicti244K → R in AAH08364 (PubMed:15489334).Curated1
Sequence conflicti254E → G in AAH07052 (PubMed:15489334).Curated1
Sequence conflicti303 – 306EDDS → G (PubMed:2557628).Curated4
Sequence conflicti303 – 306EDDS → G (PubMed:3110598).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01922539 – 119KYGKI…SPLLS → N in isoform 3. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_005831108 – 120Missing in isoform C1 and isoform 4. 3 PublicationsAdd BLAST13
Alternative sequenceiVSP_019226153 – 195Missing in isoform 4. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29063 mRNA Translation: AAA36576.1
M16342 mRNA Translation: AAA52680.1
BX161480 mRNA Translation: CAD61934.1
BX247961 mRNA Translation: CAD62300.1
BX247992 mRNA Translation: CAD62326.1
AB209527 mRNA Translation: BAD92764.1 Different initiation.
AK223517 mRNA Translation: BAD97237.1
CH471078 Genomic DNA Translation: EAW66392.1
CH471078 Genomic DNA Translation: EAW66393.1
CH471078 Genomic DNA Translation: EAW66394.1
CH471078 Genomic DNA Translation: EAW66395.1
CH471078 Genomic DNA Translation: EAW66396.1
CH471078 Genomic DNA Translation: EAW66399.1
CH471078 Genomic DNA Translation: EAW66400.1
BC003394 mRNA Translation: AAH03394.1
BC007052 mRNA Translation: AAH07052.1
BC008364 mRNA Translation: AAH08364.1
BC008423 mRNA Translation: AAH08423.1
BC066932 mRNA Translation: AAH66932.1
BC089438 mRNA Translation: AAH89438.1
BC108658 mRNA Translation: AAI08659.1
BC103758 mRNA Translation: AAI03759.1
CCDSiCCDS41915.1 [P07910-1]
CCDS45079.1 [P07910-2]
PIRiA26885
C34504
RefSeqiNP_001070910.1, NM_001077442.1 [P07910-1]
NP_001070911.1, NM_001077443.1 [P07910-2]
NP_004491.2, NM_004500.3 [P07910-2]
NP_112604.2, NM_031314.2 [P07910-1]
XP_006720188.1, XM_006720125.2 [P07910-2]
XP_011535010.1, XM_011536708.1 [P07910-1]
XP_011535011.1, XM_011536709.2 [P07910-1]
XP_011535012.1, XM_011536710.2 [P07910-1]
XP_011535013.1, XM_011536711.2 [P07910-1]
XP_011535014.1, XM_011536712.1 [P07910-2]
XP_016876741.1, XM_017021252.1 [P07910-2]
XP_016876742.1, XM_017021253.1 [P07910-2]
UniGeneiHs.508848
Hs.675546

Genome annotation databases

EnsembliENST00000420743; ENSP00000404848; ENSG00000092199 [P07910-1]
ENST00000430246; ENSP00000442816; ENSG00000092199 [P07910-2]
ENST00000553300; ENSP00000450544; ENSG00000092199 [P07910-2]
ENST00000554455; ENSP00000451291; ENSG00000092199 [P07910-1]
ENST00000554969; ENSP00000450725; ENSG00000092199 [P07910-2]
ENST00000555883; ENSP00000450629; ENSG00000092199 [P07910-4]
ENST00000556628; ENSP00000451652; ENSG00000092199 [P07910-3]
ENST00000556897; ENSP00000451176; ENSG00000092199 [P07910-2]
ENST00000557201; ENSP00000452276; ENSG00000092199 [P07910-1]
GeneIDi3183
KEGGihsa:3183
UCSCiuc001vzw.5 human [P07910-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHNRPC_HUMAN
AccessioniPrimary (citable) accession number: P07910
Secondary accession number(s): D3DS19
, D3DS22, P22628, Q53EX2, Q59FD3, Q5FWE8, Q86SF8, Q86U45, Q96HK7, Q96HM4, Q96IY5, Q9BTS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 13, 2006
Last modified: July 18, 2018
This is version 226 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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