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UniProtKB - P07908 (DNAB_BACSU)
Protein
Replication initiation and membrane attachment protein
Gene
dnaB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110.
Miscellaneous
The two mutants dna-1 (dnaBI) and dnaB-19 (dnaBII) show different characteristics for replication and membrane binding of plasmid pUB110. DnaBI is essential for both chromosome and pUB110 replication, whereas dnaBII is necessary only for chromosome replication.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 80 – 99 | CuratedAdd BLAST | 20 | |
Nucleotide bindingi | 122 – 143 | ATPCuratedAdd BLAST | 22 | |
Nucleotide bindingi | 398 – 419 | ATPCuratedAdd BLAST | 22 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA replication, synthesis of RNA primer Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU28990-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Replication initiation and membrane attachment protein |
Gene namesi | Name:dnaB Ordered Locus Names:BSU28990 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Other locations
- primosome complex Source: UniProtKB-KW
Keywords - Cellular componenti
PrimosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 113 | P → L in DnaBI; required for chromosome and plasmid replication. | 1 | |
Mutagenesisi | 379 | A → T in DnaBII; required only for chromosome replication. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079950 | 1 – 472 | Replication initiation and membrane attachment proteinAdd BLAST | 472 |
Proteomic databases
PaxDbi | P07908 |
Interactioni
Protein-protein interaction databases
IntActi | P07908, 4 interactors |
STRINGi | 224308.BSU28990 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 415 – 472 | DisorderedSequence analysisAdd BLAST | 58 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 415 – 465 | Basic and acidic residuesSequence analysisAdd BLAST | 51 |
Phylogenomic databases
eggNOGi | COG3611, Bacteria |
OMAi | KIASHWA |
PhylomeDBi | P07908 |
Family and domain databases
Gene3Di | 1.10.10.630, 1 hit |
InterProi | View protein in InterPro IPR034829, DnaD-like_sf IPR006343, DnaD_dom |
Pfami | View protein in Pfam PF07261, DnaB_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P07908-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADYWKDVLP VDPYVVKSRS MLQDIDRQII TQLYQPLIGP VAFSLYMTLW
60 70 80 90 100
GELEQNRLWG GESTHRQLMG MTQSNLKTIH QEQGKLEGIG LLKVYMKESE
110 120 130 140 150
RQERLFIYEL LPPLRPNEFF EDGMLNVFLY NRVGKTKYQQ LKQFFTHPAI
160 170 180 190 200
SEDAKDITRP FNHAFESLQP SEWKLTSDME ETVRLAEGSE YTSVGQSPSY
210 220 230 240 250
TITEDVFDFD LFLAGLSETM IPRKAMTQQV RDTIKKLSYL YGIDPLQMQN
260 270 280 290 300
VVMSAIDERD VITTEALRKA ASDWYQIERN GQLPDLVEKT QPVHLREGEQ
310 320 330 340 350
PAEEDSLDGK LIALLEAISP KKLLQDIADG TEPSKADLKI IEEIMFEQKL
360 370 380 390 400
EPGVTNVLIY YVMLKTDMKL SKNYIQKIAS HWARKKVKTV REAMKLAIEE
410 420 430 440 450
NRQYLEWAEG KTKSSKRNQK VIREEKLPDW MTEKETASDS ESGQQKLHPQ
460 470
DLEEQKKKMM EEMQKLKKYS AY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15183 Genomic DNA Translation: AAA22404.1 X04963 Genomic DNA No translation available. Z75208 Genomic DNA Translation: CAA99604.1 AF008220 Genomic DNA Translation: AAC00358.1 AL009126 Genomic DNA Translation: CAB14859.1 |
PIRi | B26580 |
RefSeqi | NP_390777.1, NC_000964.3 WP_003229464.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14859; CAB14859; BSU_28990 |
GeneIDi | 937396 |
KEGGi | bsu:BSU28990 |
PATRICi | fig|224308.179.peg.3148 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15183 Genomic DNA Translation: AAA22404.1 X04963 Genomic DNA No translation available. Z75208 Genomic DNA Translation: CAA99604.1 AF008220 Genomic DNA Translation: AAC00358.1 AL009126 Genomic DNA Translation: CAB14859.1 |
PIRi | B26580 |
RefSeqi | NP_390777.1, NC_000964.3 WP_003229464.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P07908 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P07908, 4 interactors |
STRINGi | 224308.BSU28990 |
Proteomic databases
PaxDbi | P07908 |
Genome annotation databases
EnsemblBacteriai | CAB14859; CAB14859; BSU_28990 |
GeneIDi | 937396 |
KEGGi | bsu:BSU28990 |
PATRICi | fig|224308.179.peg.3148 |
Phylogenomic databases
eggNOGi | COG3611, Bacteria |
OMAi | KIASHWA |
PhylomeDBi | P07908 |
Enzyme and pathway databases
BioCyci | BSUB:BSU28990-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.630, 1 hit |
InterProi | View protein in InterPro IPR034829, DnaD-like_sf IPR006343, DnaD_dom |
Pfami | View protein in Pfam PF07261, DnaB_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAB_BACSU | |
Accessioni | P07908Primary (citable) accession number: P07908 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | September 29, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |